Long-term Complete Remission of Decitabine-Primed Tandem CD19/CD22 CAR-T Therapy with PD-1 and BTK Inhibitors Maintenance in a Refractory PCNSL Patient

Rui Zou,Xiao Zhou,Hailing Liu,Peng Wang,Fan Xia,Liqing Kang,Lei Yu,Depei Wu,Zhengming Jin,Changju Qu 대한암학회 2023 Cancer Research and Treatment Vol.55 No.4

Primary central nervous system lymphoma (PCNSL) is a rare and aggressive non-Hodgkin’s lymphoma that affects the brain, eyes, cerebrospinal fluid, or spinal cord without systemic involvement. The outcome of patients with PCNSL is worse compared to patients with systemic diffuse large B-cell lymphoma. Given potential mortality associated with severe immune effector cell-associated neurotoxicity syndrome (ICANS), patients with PCNSL have been excluded from most clinical trials involving chimeric antigen receptor T-cell (CAR-T) therapy initially. Here, we report for the first time to apply decitabine-primed tandem CD19/CD22 dual-targeted CAR-T therapy with programmed cell death-1 (PD-1) and Bruton’s tyrosine kinase (BTK) inhibitors maintenance in one patient with multiline-resistant refractory PCNSL and the patient has maintained complete remission (CR) for a 35-month follow-up period. This case represents the first successful treatment of multiline resistant refractory PCNSL with long-term CR and without inducing ICANS under tandem CD19/CD22 bispecific CAR-T therapy followed by maintenance therapy with PD-1 and BTK inhibitors. This study shows tremendous potential in the treatment of PCNSL and offers a look toward ongoing clinical studies.

An Interference Restraint Algorithm in OFDM-Based Cognitive Radio

Ming Rui Zou,Cheng Shi Zhao,Bin Shen,Ho Hwan Park,Kyung Sup Kwak 한국통신학회 2008 한국통신학회 학술대회 및 강연회 Vol.2008 No.4

Prognostic Significance of Hes-1, a Downstream Target of Notch Signaling in Hepatocellular Carcinoma

Zou, Jing-Huai,Xue, Tong-Chun,Sun, Chun,Li, Yan,Liu, Bin-Bin,Sun, Rui-Xia,Chen, Jie,Ren, Zheng-Gang,Ye, Sheng-Long Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Background: Hairy and enhancer of split 1 (Hes-1) protein is a downstream target of Notch signaling and is a basic helix-loop-helix transcriptional repressor. However, definitive evidence for a role in hepatocellular carcinoma (HCC) cells has not been reported. Here, Hes-1 was revealed to an important component of the Notch signaling cascade in HCC cell lines possessing different potential for lung metastasis. Materials and Methods: RNAi mediated by plasmid constructs was used to analyze the role of Hes-1 in MHCC-97L HCC cells by assessing proliferation, apoptosis, cell migration and matrigel invasion following transfection. Hes-1 protein expression analysis in HCC tissue was also conducted by immunohistochemistry. Results: Our studies revealed that Hes-1 was decreased in HCC cell lines with higher lung metastasis potential at both the mRNA and protein levels. Down-regulation of the Hes-1 gene in MHCC-97L cells resulted in increased cell proliferation, reduced apoptosis and increased migration and invasion. Conclusions: Hes-1 has potential prognostic value in post-surgical HCC patients and may be an independent prognostic indicator for overall survival and tumor recurrence. These findings have important implications for understanding the mechanisms by which Hes-1 participates in tumor proliferation and invasion.

Beta-asarone Induces LoVo Colon Cancer Cell Apoptosis by Up-regulation of Caspases through a Mitochondrial Pathway in vitro and in vivo

Zou, Xi,Liu, Shen-Lin,Zhou, Jin-Yong,Wu, Jian,Ling, Bo-Fan,Wang, Rui-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Beta-asarone is one of the main bioactive constituents in traditional Chinese medicine Acorus calamu. Previous studies have shown that it has antifungal and anthelmintic activities. However, little is known about its anticancer effects. This study aimed to determine inhibitory effects on LoVo colon cancer cell proliferation and to clarify the underlying mechanisms in vitro and in vivo. Dose-response and time-course anti-proliferation effects were examined by MTT assay. Our results demonstrated that LoVo cell viability showed dose- and time-dependence on ${\beta}$-asarone. We further assessed anti-proliferation effects as ${\beta}$-asarone-induced apoptosis by annexin V-fluorescein isothiocyanate/propidium iodide assay usinga flow cytometer and observed characteristic nuclear fragmentation and chromatin condensation of apoptosis by microscopy. Moreover, we found the apoptosis to be induced through the mitochondrial/caspase pathway by decreasing mitochondrial membrane potential (MMP) and reducing the Bcl-2-to-Bax ratio, in addition to activating the caspase-9 and caspase-3 cascades. Additionally, the apoptosis could be inhibited by a pan-caspase inhibitor, carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone (Z-VAD-FMK). When nude mice bearing LoVo tumor xenografts were treated with ${\beta}$-asarone, tumor volumes were reduced and terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) assays of excised tissue also demonstrated apoptotic changes. Taken together, these findings for the first time provide evidence that ${\beta}$-asarone can suppress the growth of colon cancer and the induced apoptosis is possibly mediated through mitochondria/caspase pathways.

Template Synthesis of Nitrogen-Doped Short Tubular Carbons with Big Inner Diameter and their Application in Electrochemical Sensing

Rui Cheng,Qiong Zou,Xiaohua Zhang,Chunhui Xiao,Longfei Sun,Jinhua Chen 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.8

Nitrogen-doped short tubular carbons (N-STCs) with big inner diameter have been successfully synthesized via carbonization of polydopamine (PDA) wrapped halloysite nanotubes (HNTs). The obtained N-STCs have average length of 0.3 μm with big inner diameter (50 nm), thin wall (2-3 nm) and large surface area (776 m2 g−1), and show excellent electrochemical properties. As an example in electrochemical applications, N-STCs were used to electrochemically detect hydrogen peroxide (H2O2) and glucose. The results showed that the N-STCs modified glassy carbon (N-STCs/GC) electrode had much better analytical performance (lower detection limit and wider linear range) compared to the acid-treated carbon nanotubes (AO-CNTs) based GC electrode. The unique structure endows N-STCs the enhanced electrochemical performance and promising applications in electrochemical sensing.

Validation of a Strategy to Predict Secant Shear Modulus and Damping of Soils with an Elastoplastic Model

Rui Carrilho Gomes,Jaime A. Santos,Arézou Modaressi-Farahmand Razavi,Fernando Lopez-Caballero 대한토목학회 2016 KSCE JOURNAL OF CIVIL ENGINEERING Vol.20 No.2

The assessment of seismic site effects such as ground motion and permanent displacement requires the accurate description of the soil's stress-strain-strength relationship under irregular cyclic loading from small to medium and at large strains. The main objective of this paper is to enhance and validate the performance of an elastoplastic constitutive law in modelling non-linear soil behaviour, with particular attention to the stiffness and damping evolution with deformation. First, a simple and rational strategy is presented to derive model parameters related to shear hardening based on experimental data. Secondly, as the elastoplastic law tends to overestimate damping in the large strain range in comparison with experimental data (Ishihara, 1996; Puzrin, 2012), a new parameter is introduced in the model to overcome this issue. The modified model response exhibits lower stiffness than the standard one. For sands, an effective reduction of the damping factor is achieved and good agreement is obtained for hysteretic loop and straindependent stiffness and damping curves. For clays, the reduction in damping is also achieved for large strains, but it also has a significant effect on the soil stiffness. Finally, numerical simulations of one-dimensional ground seismic response show that for sands the new parameter has no visible effect on the seismic soil response due to maximum shear strain level achieved, while for clays the reduction in both damping and stiffness occurs.

Complete mitochondrial genome of a kind of snakehead fish Channa siamensis and its phylogenetic consideration

Rui Li,Gang Wang,Zheng‑Yong Wen,Yuan‑Chao Zou,Chuan‑Jie Qin,Yu Luo,Jun Wang,Gui‑Hong Chen 한국유전학회 2019 Genes & Genomics Vol.41 No.2

The snakehead fish, Channa siamensis, belongs to the genus of Channa (perciformes: Channidae) and was first reported by Günther in 1861. Despite it has been described approximately for 15 decades, the genetic information is limited and the taxon status of this kind of fish is still unclear. The primary objective of this study is to get more genomic data and calculate the taxon location of this kind of fish. The next generation sequencing method was used to obtain the whole mitochondrial DNA information, and bioinformatic analysis was performed to investigate the evolutionary status and taxon location of C. siamensis. The circular mitochondrial DNA was 16,570 bp in length, and which showed typical piscine structure and arrangement. The overall nucleotide composition was 29.28% A, 24.72% T, 30.71% C, 15.29% G, with 54.1% AT, respectively. Phylogenetic analyses using concatenated amino acid and nucleotide sequences of the 13 protein-coding genes with two different methods (Maximum likelihood and Bayesian analysis) both highly supported C. siamensis belongs to the genus Channa and shows a close relationship with C. micropeltes. These data will provide more useful information for a better understanding of the mitochondrial genomic diversities and evolution in fish as well as novel genetic markers for studying population genetics and species identification.

Characterization of the complete chloroplast genome sequence of <i>Hypericum petiolulatum</i> (Hypericaceae)

ZOU Le,BAI Rui-Zhu,XIANG Chun-Lei,LI Lu 한국식물분류학회 2024 식물 분류학회지 Vol.54 No.1

<i>Hypericum</i> L. (Hypericaceae) is one of the best-selling herbal medicines in the world comprising ca. 500 species of herbs, shrubs, and small trees. <i>Hypericum petiolulatum</i> Hook. f. & Thomson ex Dyer is widely distributed in China, Vietnam, Myanmar, Nepal, India, Malaysia, and Bhutan and is used as a traditional herb to treat hemoptysis and inflammation. In this study, we sequenced and assembled the complete chloroplast (cp) genome of <i>H. petiolulatum</i>. The complete plastome of <i>H. petiolulatum</i> was 136,105 bp in length, with a large single copy region (LSC) of 93,709 bp, a small single copy region (SSC) of 11088 bp and two identical inverted repeats (IRs) of 15,654 bp. The overall GC content of the plastome was 37.0%, while GC contents of the LSC, SSC, and each IR were 35.5%, 31.0%, and 43.8%, respectively. In addition, 116 genes consisting of 76 protein-coding genes, six ribosomal RNA genes, and 34 transfer RNA genes were identified. A phylogenetic analysis of 14 taxa inferred based on cp genome sequences revealed a close relationship between <i>H. petiolulatum</i> and <i>H. perforatum</i>. The complete cp genome sequence of <i>H. petiolulatum</i> reported in this paper will facilitate population and phylogenomics studies of this medicinal plant group.

Diffusion mechanism of deep shale gas and its carbon isotope fractionation: a combined simulated and mathematical analysis

Yu Zou,Guojian Wang,Cheng Tao,Rui Zhou,Shangqing Zhang,Dongna Liu,Xiaohui Lin,Xuying Zheng 한국지질과학협의회 2023 Geosciences Journal Vol.27 No.3

Unlike the release pressure process in shale gas exploitation, in this study, the concentration diffusion process is deeply discussed under constant temperature-pressure conditions, which aims to evaluate the preservation conditions of shale gas reservoirs in combination with isotopic fractionation characteristics. The isobaric diffusion experiment emphasized that decreasing pressure can lead to enhanced diffusion and isotope fractionation. The established mathematical model not only confirms the results of the simulating experiment, but also suggests that the contributions of Fick, Knudsen and surface diffusion to the migration of methane (including 12CH4 and 13CH4) vary in the geological evolution of shale reservoirs, which are mainly controlled by the pore system and gas pressure. Based on the analysis of specific samples of Longmaxi shale (r = 11.7 nm) and Niutitang shale (r = 1.3 nm), we propose that: 1) high pressure condition (> 20 MPa) can significantly limit Knudsen diffusion, which is the reason for reducing the total diffusion coefficient (DTotal) and isotope fractionation, while low pressure (< 10 MPa) and small pore radius (ca. 1 nm) can strengthen the fractionation; 2) pore connectivity and heterogeneity can lead to an order of magnitude change in DTotal, while the influence of temperature is relatively small. According to the mathematical model, it can be expected that enhanced dissipation intensity and isotope fractionation of shale gas may occur in the process of basin uplift and pressure relief, which can potentially be used for the evaluation of reservoir preservation.

Synthesis, and antioxidant, thrombin-inhibitory, and anticancer activities of hydroxybenzamide derivatives

Jin-Rui Wei,Kun Liu,Faquan Lin,Chunping He,Xuan Luo,Jiajia Zou,Wei He,Wenqian Nong,Cui-Wu Lin 한국응용생명화학회 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.2

Four hydroxybenzamide derivatives were synthesized from protocatechuic acid. The antioxidant activities of these derivatives were evaluated in vitro using DPPH radical scavenging and reducing power assays. In addition, these compounds were subjected to a chromogenic thrombin-inhibitory assay using Chromozym-TH as the substrate. The anticancer effects of these derivatives were also investigated using the MTT assay in three human cancer cell lines. Four derivatives exhibited excellent DPPH radical scavenging activity and redox potential. Their highly inhibitory effect against thrombin may allow them to be utilized as novel micromolecule thrombin inhibitors. Furthermore, four derivatives demonstrated different inhibitory activities in three human cancer cell lines but showed minimal harmful effects on normal HUVEC cells. Therefore, hydroxybenzamide derivatives indicate the superior antioxidant, thrombin-inhibitory, and anticancer activities along with the excellent biocompatibility, which have the potential application in therapeutic interventions and disease prevention of cancer, thrombotic diseases, and aging.