First Report on Bacterial Heart Rot of Garlic Caused by Pseudomonas fluorescens in China

Bin Li,Rong Rong Yu,Shan Hong Yu,Wen Qiu,Yuan Fang,Guan Lin Xie 한국식물병리학회 2009 Plant Pathology Journal Vol.25 No.1

An unreported disease of garlic was observed in commercial fields in Jiangsu province, China. The symptoms started as water soaked lesions at the base of the leaves. Later, water-soaked areas developed on stems and spread to the internal tissues, followed by yellowing and necrosis along leaf edges and soft rot of the stems. The causal organism isolated from symptomatic plants was identified as Pseudomonas fluorescens based on its biochemical and physiological characteristics and confirmed by the cellular fatty acid composition and Biolog data as well as 16S rRNA gene sequence analysis. The bacterial isolates caused similar symptoms when inoculated onto garlic plants. In addition, leek and shallot were susceptible to the P. fluorescens pathogen. However, the P. fluorescens pathogen failed to cause any symptoms when it was inoculated onto 15 other plants. This is the first report of a bacterial disease of garlic caused by P. fluorescens in China.

Ursolic Acid-induced Apoptosis in K562 Cells Involving Upregulation of PTEN Gene Expression and Inactivation of the PI3K/Akt Pathway

Bin Wu,Zhuo-gang Liu,Xu Wang,Zuo-fei Chi,Rong Hu,Rong Zhang,Wei Yang 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.3

Ursolic acid (UA), a pentacyclic triterpenoid derived from a variety of medicinal plants, exhibits potent anticancer activity against many types of cancer cells. However, the anticancer mechanism of UA is not clearly understood. Suppression of phosphatase and a tensin homolog deleted on chromosome 10 (PTEN) gene expression leading to activation of the phosphatidylinositol-3-OH kinase (PI3K)/Akt pathway has been observed in many cancers including leukemia, making the PTEN gene and PI3K/Akt pathway a central target for cancer therapy. Here, we demonstrated that UA was able to inhibit growth, induce apoptosis in a human chronic myelogenous leukemia cell line (K562 cells) via upregulation of PTEN gene expression, inhibit Akt kinase activity, change mitochondrial transmembrane potential and reduce the release of cytochrome c and the activity of caspases. These results suggest that UA may elicit its strong antitumor effects via upregulation of the PTEN gene and inhibition of the PI3K/Akt pathway.

Seismic performance of beam-to- SST column connection with external diaphragm

Bin Rong,Shuhao Yin,Ruoyu Zhang,Lei Wang,Ziheng Yang,Hongtao Li,Wenyu Wan 국제구조공학회 2020 Steel and Composite Structures, An International J Vol.37 No.6

This paper aims to study the seismic performance of external diaphragm connection between SST (square steel tube) column and H-shaped beam through experimental and analytical study involving finite element (FE) method and theoretical analysis. In the experimental study, three external diaphragm connection specimens with weak panel zone were tested under axial pressure on the top of the column and antisymmetric cyclic loads at the beam end to investigate the seismic performance of the panel zone. The hysteretic behavior, failure mode, stiffness and ductility of the specimens were discussed. Key point to be explored was the influence of the thickness of the steel tube flange on the shear capacity of the specimens. In the analytical study, three simplified FE models were developed to simulate the seismic behavior of the specimens for further analysis on the influence of steel tube flange. Finally, four existing calculation formulas for the shear capacity of the external diaphragm connection were evaluated through comparisons with the results of experiments and FE analysis, and application suggestions were put forward.

Computational Model of the Elastic Stiff ness of Panel Zone in Diaphragm-Through Connections

Bin Rong,Ziheng Yang,Ruoyu Zhang,Changxi Feng 한국강구조학회 2019 International Journal of Steel Structures Vol.19 No.5

The elastic stiff ness of the panel zone in diaphragm-through connection between square steel tubular column and H-shaped steel beam subjected to lateral loads is studied in this paper. Through analyzing the deformation mechanism of diaphragmthrough connection, the rotation of the panel zone is found to be the superposition of bending deformation of the column and shear deformation of the panel zone. Based on the mechanical model, the computational method of the elastic stiff ness of the panel zone in the diaphragm-though connection is developed. To verify the computational method, three cruciform specimens were tested subjected to a set of anti-symmetrical cyclic load at the beam tips. Based on the test results, the developed fi nite element model is verifi ed and used in the follow-up parametric analysis to check the applicability of the proposed computational method. Key variables involved are the width of column, thickness of column and the height-towidth ratio of the core tube.

Tensile Capacity of Concrete-filled Square Tubular Columns Connection with External Stiffening Ring

Bin Rong,Xinxin Quan,Ruoyu Zhang,Guangchao You 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.10

A static tensile experiment was carried out to investigate the flexural bearing capacity of external stiffening ring joints between concrete-filled square steel tubular column and steel beam. All of four specimens showed similar failure modes that external stiffening ring and tube flange were torn up. Nonlinear finite element model was developed with ABAQUS software to simulate the test and analyze the mechanism properties of specimens. Comparison of yield and ultimate capacity indicated that finite element analysis is valid. Moreover, on the basis of the failure modes, the distribution of stress, the deformation features and load transferring paths, which were achieved from experiments and numerical simulation, a theoretical method taking tensile membrane action of tube faces into consideration to evaluate the bearing capacity was proposed with help of yield line theory. The outcome of comparison between theoretical and experimental values clarify good applicability of this analytical method.

Experimental and theoretical studies on SHS column connection with external stiffening ring under static tension load

Bin Rong,Guangchao You,Ruoyu Zhang,Xu Ma,Xinxin Quan 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.28 No.2

In order to investigate mechanical properties in the core area of Square Hollow Section(SHS) column connection with external stiffening ring, four specimens were tested under the static tension load. The failure modes, load-displacement curves and strain distribution were analyzed to study the mechanical properties and the load transfer mechanism of the core area of connections. The connections behave good ductility and load-bearing capacity under the static tension load. Parametric analysis was also conducted, in which the thickness of steel tube, extended width and thickness of the stiffening ring were considered as the parameters to investigate the effects on mechanical properties of the connections. Based on the experimental results, an analytical method for the bearing capacity of connection with external stiffening ring under the static tension load was proposed. The theoretical results and the experimental results are in good agreement, which indicates that the theoretical calculation method of the bearing capacity is advisable.

Chromatographic Behavior of Proteins on Stationary Phase with Aminocarboxy Ligand

Rong Li,Ming-Yang Ju,Bin Chen,Qing-Yuan Sun,Guo-Liang Chen,Mei Shi,Xiao-Gang Wang,Jian-Bin Zheng 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.2

An aminocarboxy aspartic acid-bonded silica (Asp-Silica) stationary phase was synthesized using L-aspartic acid as ligand and silica gel as matrix. The standard protein mixtures were separated with prepared chromatographic column. The effects of solution pH, salt concentration and metal ion on the retention of proteins were examined, and also compared with traditional iminodiacetic acid-bonded silica (IDA-Silica) column. The results show that Asp-Silica column exhibited an excellent separation performance for proteins. The retention of proteins on Asp-Silica stationary phase was consistent with electrostatic characteristic of cation-exchange. The stationary phase displayed typical metal chelate property after fixing copper ion (II) on Asp-Silica. Under competitive eluting condition, protein mixtures were effectively isolated. Asp ligand showed better ion-exchange and metal chelating properties as compared with IDA ligand.

주물공정 악취·분진 동시 처리를 위한 여과 집진장치 개발연구

허영빈 ( Rong Bin Xu ),김태형 ( Tae Hyeung Kim ),하현철 ( Hyun Chul Ha ),박승욱 ( Cheng Xu Piao ) 한국환경과학회 2014 한국환경과학회지 Vol.23 No.8

Foundry has an important economic value in the industry. However, the generation of air pollutants like particulate and odor are serious. Due to the unavoidable usage of molding sand, particulate occurs in almost all the processes. That accounts for the majority of respirable dust in the size less than 10 ㎛. As well as particulate, over 22 species of odor-causing gases and VOCs including hydrogen sulfide and ammonia are occurred. Therefore, the development of equipment that can simultaneously remove TVOC and particulate is regarded as an essential research. In this study, the spraying absorbent system was connected with the shear bag filter for the purpose to remove TVOC and particulate simultaneously. Maximization of process efficiency for the affective factors like the powder combination and injection method is conducted. The experiment was performed at the de-molding process of one foundry plant. Through these devices, the removal efficiency of more than 95% for TVOC was achieved with the absorbent that composed by 800 mesh Activated carbon (80%) and 300 mesh zeolite (20%). Also, the durability and economic evaluation were assessed. In the result of Durability assessment, the available recovery to maintain the deodorizing effect at 90% was counted to 350 degree.

Chromatographic Behavior of Proteins on Stationary Phase with Aminocarboxy Ligand

Li, Rong,Ju, Ming-Yang,Chen, Bin,Sun, Qing-Yuan,Chen, Guo-Liang,Shi, Mei,Wang, Xiao-Gang,Zheng, Jian-Bin Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.2

An aminocarboxy aspartic acid-bonded silica (Asp-Silica) stationary phase was synthesized using L-aspartic acid as ligand and silica gel as matrix. The standard protein mixtures were separated with prepared chromatographic column. The effects of solution pH, salt concentration and metal ion on the retention of proteins were examined, and also compared with traditional iminodiacetic acid-bonded silica (IDA-Silica) column. The results show that Asp-Silica column exhibited an excellent separation performance for proteins. The retention of proteins on Asp-Silica stationary phase was consistent with electrostatic characteristic of cation-exchange. The stationary phase displayed typical metal chelate property after fixing copper ion (II) on Asp-Silica. Under competitive eluting condition, protein mixtures were effectively isolated. Asp ligand showed better ion-exchange and metal chelating properties as compared with IDA ligand.

First Report on Bacterial Heart Rot of Garlic Caused by Pseudomonas fluorescens in China

Li, Bin,Yu, Rong Rong,Yu, Shan Hong,Qiu, Wen,Fang, Yuan,Xie, Guan Lin The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.1

An unreported disease of garlic was observed in commercial fields in Jiangsu province, China. The symptoms started as water soaked lesions at the base of the leaves. Later, water-soaked areas developed on stems and spread to the internal tissues, followed by yellowing and necrosis along leaf edges and soft rot of the stems. The causal organism isolated from symptomatic plants was identified as Pseudomonas fluorescens based on its biochemical and physiological characteristics and confirmed by the cellular fatty acid composition and Biolog data as well as 168 rRNA gene sequence analysis. The bacterial isolates caused similar symptoms when inoculated onto garlic plants. In addition, leek and shallot were susceptible to the P. fluorescens pathogen. However, the P. fluorescens pathogen failed to cause any symptoms when it was inoculated onto 15 other plants. This is the first report of a bacterial disease of garlic caused by P. fluorescens in China.