Protective effects of l-theanine on rats with dextran sulfate sodium-induced infl ammatory bowel disease

Ling Chen,Wen-jun Xiao,Qiong-xian Yan,Zhi-hua Gong,Sheng Zhang,Li Zeng,Ming Yang,Yan-he Zhou 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.8

The aim of this study is to evaluate the antiinflammatory and protective eff ects of L -theanine in infl ammatorybowel disease (IBD) and to identify the underlyingmolecular mechanisms. Rats were pre-treated with L -theanineat 0, 50, 200, or 800 mg/kg/day. IBD was induced inrats using dextran sulfate sodium (DSS). Histopathologicalanalysis suggests that L -theanine can suppress DSS-inducedIBD with signifi cant inhibition of infl ammation in large andsmall intestinal tissues. Moreover, the 200 mg/kg/day L -theanine-treated DSS group had higher body and small intestineweights, a lower disease activity index and expression ofinfl ammatory factors than the DSS group without pre-treatment. In RNA sequencing and tandem mass tag labelinganalyses, large number of mRNAs and proteins expressionlevel diff ered when compared with the DSS-induced ratswith and without 200 mg/kg/day L -theanine pre-treatment. Moreover, Kyoto Encyclopedia of Genes and Genomes pathwayanalysis indicates the anti-infl ammatory activities ofL -theanine in DSS-induced IBD, with a high representationof genes in “Cholesterol metabolism” and “Retinol metabolism”pathways. Analysis of protein–protein interaction networksfurther indicates the involvement of these two pathways. These studies suggest that medium-dose L -theaninepre-treatment could ameliorate DSS-induced IBD throughmolecular mechanisms involving cholesterol and retinolmetabolism.

Interactions of xanthate with pyrite and galena surfaces in the presence and absence of oxygen

Jian-Hua Chen,Yu-Qiong Li,Li-Hong Lan,Jin Guo 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.1

In this study, first-principles calculations were performed to investigate the interactions of hydrogenxanthate (HOCS₂-) with pyrite (1 0 0) and galena (1 0 0) surfaces. The calculation results suggest thatstrong chemisorption of HOCS₂- occurs at Fe sites on the pyrite (1 0 0) surface, whereas its interaction atPb sites on the galena (1 0 0) surface is weak. The interaction of xanthate with oxidized pyrite surface isweakened while the interaction of xanthate with oxidized galena surface is enhanced. The hydration ofxanthate and mineral surfaces is discussed. Dixanthogen could be formed on the pyrite surface but noton the galena surface.

Matrix Metalloproteinase-9 as a Prognostic Factor in Gastric Cancer: A Meta-Analysis

Zhang, Qiong-Wen,Liu, Lei,Chen, Ru,Wei, Yu-Quan,Li, Ping,Shi, Hua-Shan,Zhao, Yu-Wei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Background: Matrix metalloproteinase-9 (MMP-9) is associated with disruption of basement membranes of blood vessels and promotion of metastasis through the lymphatics. However, its prognostic value for survival in patients with gastric cancer remains controversial. Method: We therefore conducted a meta-analysis of the published literature in order to clarify the impact of MMP-9. Clinical studies were selected for further analysis if they provided an independent assessment of MMP-9 in gastric cancer and reported analysis of survival data according to MMP-9 expression. Results: A total of 11 studies, covering 1700 patients, were included for meta-analysis. A summary hazard ratio (HR) of all studies and sub-group hazard ratios were calculated. The combined HR suggested that a positive MMP-9 expression had an impact on overall survival: 1.25 (95% confidence interval 1.11-1.40) in all eligible studies; 1.13 (1.06-1.20) in 8 studies detecting MMP-9 by immunohistochemistry; 1.36 (1.12-1.65) in 7 studies from Asia. Only one study for DFS showed a significant impact on disease free survival (HR 1.73, 95%CI 1.27-2.34). Conclusions: Our findings suggested that MMP-9 protein expression might be a factor for a poor prognosis in patients with gastric cancer. However, the association was rather weak, so that more prospective studies should further explore the prognostic impact of MMP-9 mRNA and correlations between MMP-9 and clinicopathological characteristics.

QTL mapping and identification of candidate genes for cold tolerance at the germination stage in wild rice

Pan Ying-Hua,Nong Bao-Xuan,Chen Lei,Yang Xing-Hai,Xia Xiu-Zhong,Zhang Zong-Qiong,Qing Dong-Jin,Gao Ju,Huang Cheng-Cui,Li Dan-Ting,Deng Guo-Fu 한국유전학회 2023 Genes & Genomics Vol.45 No.7

Background Cold damage stress significantly affects rice growth (germination and seedling) and causes serious losses in yield in temperate and high-altitude areas around the globe. Objective This study aimed to explore the cold tolerance (CT) locus of rice and create new cold-tolerant germplasm. We constructed a chromosome segment substitution line (CSSL) with strong CT and fine mapped quantitative trait loci (QTLs) associated with CT by performing the whole-genome resequencing of CSSL with phenotypes under cold treatment. Methods A chromosome CSSL, including 271 lines from a cross between the cold-tolerant wild rice Y11 (Oryza rufipogon Griff.) and the cold-sensitive rice variety GH998, was developed to map QTLs conferring CT at the germination stage. The whole-genome resequencing was performed on CSSL for mapping QTLs of associated with CT at the germination stage. Results A high-density linkage map of the CSSLs was developed using the whole-genome resequencing of 1484 bins. The QTL analysis using 615,466 single-nucleotide polymorphisms (SNPs) led to the identification of 2 QTLs related to germination rate at low-temperature on chromosome 8 (qCTG-8) and chromosome 11 (qCTG-11). The qCTG-8 and qCTG-11 explained 14.55% and 14.31% of the total phenotypic variation, respectively. We narrowed down qCTG-8 and qCTG-11 to 195.5 and 78.83-kb regions, respectively. The expression patterns of important candidate genes in different tissues, and of RNA-sequencing (RNA-seq) in CSSLs, were identified based on gene sequences in qCTG-8 and qCTG-11 cold-induced expression analysis. LOC_Os08g01120 and LOC_Os08g01390 were identified as candidate genes in qCTG-8, and LOC_Os11g32880 was identified as a candidate gene in qCTG-11. Conclusions This study demonstrated a general method that could be used to identify useful loci and genes in wild rice and aid in the future cloning of candidate genes of qCTG-8 and qCTG-11. The CSSLs with strong CT were supported for breeding cold-tolerant rice varieties.

Synthesis and DNA-binding Properties of Trehalose-tethered Monomeric and Dimeric Berberines

Yong-Min Wang,Chun-Qiong Zhou,Jin-Xiang Chen,Wen-Hua Chen 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.3

Trehalose-tethered monomeric and dimeric berberines were synthesized in 50% and 30% from the reaction of berberrubine with 6-tosyl-α,α'-trehalose and 6,6'-ditosyl-α,α'-trehalose, respectively, and fully characterized by MS (HR and ESI) and NMR (1H, 13C, COSY and HSQC). Spectrophotometric and spectrofluorimetric titrations indicated that compared with berberine, trehalose-tethered monomeric berberine had comparable DNA-binding affinity toward calf-thymus DNA, whereas trehalose-spaced dimeric berberine exhibited higher DNA-binding affinity. The potential application of these conjugates is also briefly discussed.

Organization process of the hierarchical structures in microbially synthesized polyhydroxyalkanoates

Jun Xu,Bao-Hua Guo,Qiong Wu,Jin-Chun Chen,Guo-Qiang Chen,Jian-Jun Zhou,Yong Jiang,Lin Li 한국물리학회 2007 Current Applied Physics Vol.7 No.s1

cess of the high-order structures in biomaterials. Real-time Fourier transform infrared spectroscopy and optical microscopy demon-strated that helical segments formed along with the spherulite growth. Atomic force microscopy revealed the details of growth,twisting and branching of lamellar crystals. Cooperative packing of these twisting lamellae led to regular banded spherulites observedunder polarized light microscopy. Real-time observation on the crystallization process provided richer information than the characterization of the final structures; consequently, it provides deeper insight into the organization mechanism of the hierarchical structures.

Mapping QTLs for Grain Quality Using an Introgression Line Population from a Cross between Oryza sativa and O. rufipogon

Yuan, Ping-Rong,Kim, Hyun-Jung,Chen, Qiong-Hua,Ju, Hong-Guang,Ji, Shi-Dong,Ahn, Sang-Nag 한국작물학회 2010 Journal of crop science and biotechnology Vol.13 No.4

Quantitative trait loci (QTL) associated with four milling recovery properties, two chemical properties, six paste properties of grain, and six textural parameters of cooked rice were identified using an introgression line (IL) population of rice developed from an interspecific cross over two years. The IL population consisted of 121 lines from a cross between wild rice (O. rufipogon Griff.) and a japonica cultivar. A total of 28 QTLs were identified for the 14 traits using inter mapping. Of these, 10 were significant over two years indicating that these QTLs are stable across years and environments. For eight (21 %) of the QTLs identified, the O. rufipogon-derived alleles contributed a desirable effect on amylose content, protein content, minimum viscosity, final viscosity, and consistency. Among these, pc3 for protein content and ac7 for amylose content were significant in both years and showed an $R^2$ value of 25.5 and 30.9%, respectively. The markers closely associated with these useful alleles can be used to trace the inheritance of specific chromosome segments in the IL population and also offer a starting point for map-based cloning of genes underlying these traits.

QTL Dissection of Agronomic and Domestication Traits Using Introgression Lines Carrying Wild Rice (Oryza rufipogon Griff.) Segments in Cultivated Rice (O. sativa L.) Background

Yuan, Ping-Rong,Kim, Hyun-Jung,Chen, Qiong-Hua,Ju, Hong-Guang,Lee, Seung-Joon,Ji, Shi-Dong,Ahn, Sang-Nag 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.4

The nearly isogenic nature of the introgression lines (ILs) provides a relative advantage over other segregating populations in the rapid implementation of pyramiding approach through crosses and marker analysis. A set of 126 ILs carrying various introgressed segments from a presumed wild progenitor, Oryza rufipogon Griff. Acc. W1944 in the background of an elite Korea japonica cultivar (O. sativa L.), Hwayeongbyeo, was constructed using the marker assisted selection (MAS) technique combined with repeated backcrosses. The 126 ILs have different W1944 segments on each chromosome, with 100% coverage of wild segments on chromosome 1, while for chromosome 10, the coverage was only 33.3%. The mean number of homozygous and heterozygous donor segments were 3 (ranging from 0 to 7) and 4.7 (ranging from 1 to 14.5), respectively, and 31.6% of introgressed segments had sizes of less than 10.5 cM. A total of 41 quantitative trait loci (QTLs) and two loci associated with pericarp coloration were identified. The number of QTLs per trait ranged from 2 to 6. Phenotypic variance associated with each QTL varied from 9.1 to 52.2%, with an average of 17.1%. For 11 (26.8%) of the QTLs detected in this study, the O. rufipogon-derived alleles contributed the desired agronomic effect despite the overall inferior characteristics of the wild phenotype. Favorable alleles from the O. rufipogon accession were identified for panicle number, panicle length, days to heading, secondary branches, spikelets per panicle, and 1,000-grain weight.

Synthesis and DNA-binding Properties of Trehalose-tethered Monomeric and Dimeric Berberines

Wang, Yong-Min,Zhou, Chun-Qiong,Chen, Jin-Xiang,Chen, Wen-Hua Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.3

Trehalose-tethered monomeric and dimeric berberines were synthesized in 50% and 30% from the reaction of berberrubine with 6-tosyl-${\alpha}$,${\alpha}^{\prime}$-trehalose and 6,6'-ditosyl-${\alpha}$,${\alpha}^{\prime}$-trehalose, respectively, and fully characterized by MS (HR and ESI) and NMR ($^1H$, $^{13}C$, COSY and HSQC). Spectrophotometric and spectrofluorimetric titrations indicated that compared with berberine, trehalose-tethered monomeric berberine had comparable DNA-binding affinity toward calf-thymus DNA, whereas trehalose-spaced dimeric berberine exhibited higher DNA-binding affinity. The potential application of these conjugates is also briefly discussed.

QTLDissection of Agronomic and Domestication Traits Using Introgression Lines Carrying Wild Rice (Oryza rufipogon Griff.) Segments in Cultivated Rice (O. sativa L.) Background

Ping-Rong Yuan,Hyun-Jung Kim,Qiong-Hua Chen,Hong-Guang Ju,Seung-Joon Lee,Shi-Dong Ji,안상낙 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.4

The nearly isogenic nature of the introgression lines (ILs) provides a relative advantage over other segregating populations in therapid implementation of pyramiding approach through crosses and marker analysis. A set of 126 ILs carrying various introgressedsegments from a presumed wild progenitor, Oryza rufipogon Griff. Acc. W1944 in the background of an elite Korea japonica cultivar(O. sativa L.), Hwayeongbyeo, was constructed using the marker assisted selection (MAS) technique combined with repeatedbackcrosses. The 126 ILs have different W1944 segments on each chromosome, with 100% coverage of wild segments on chromosome1, while for chromosome 10, the coverage was only 33.3%. The mean number of homozygous and heterozygous donor segmentswere 3 (ranging from 0 to 7) and 4.7 (ranging from 1 to 14.5), respectively, and 31.6% of introgressed segments had sizes ofless than 10.5 cM. A total of 41 quantitative trait loci (QTLs) and two loci associated with pericarp coloration were identified. Thenumber of QTLs per trait ranged from 2 to 6. Phenotypic variance associated with each QTL varied from 9.1 to 52.2%, with an averageof 17.1%. For 11 (26.8%) of the QTLs detected in this study, the O. rufipogon-derived alleles contributed the desired agronomiceffect despite the overall inferior characteristics of the wild phenotype. Favorable alleles from the O. rufipogon accession were identifiedfor panicle number, panicle length, days to heading, secondary branches, spikelets per panicle, and 1,000-grain weight.