A nonNMDA antagonist, GYKI 52466 improves microscopic O₂balance in the cortex during focal cerebral ischemia

Chi, Oak Z.,Chang, Qiang,Wang, Guolin,Liu, Xia,Harvey R. Weiss 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Oak Z.Chi,Qiang Chang, Guolin Wang*, Xia Liu, Harvey R. Weiss□.Deprtments of Anesthesai, Departments of Physiology and Biophysics, University of Medicne and Dentisrty of New Jersey,Robert Wood Johnson Medical School, New Brunswick, New Jersey, USA and*Department of Anesthesia, Medical University, Tianjing, People's Republic of China. A non-NMDA antagonist, GYKI 52466 improves microscopic O² balance in the cortex during focal cerebral ischemia. Proceedings of International Symposium on East-West Medicine, Seoul. 172-182, 1999.-This study was performed to test whether GYKI 52466, a non-NMDA receptor antagonist, would improve microregional oxygen supply and consumption balance in the focal cerebral ischemic area. Rats were anesthetized with 1.4% isoflurance. For the GYKI Group (n=8), 19 min before middle cerebral artery (MCA) occlusion, a bolus of 5mg/kg of GYKI 52466 iv was administered and was followed by an infusion of 5mg/kg/hr. For the control Group(n=8), the same volume of the vehicle was administered. One hour after MCA occlusion, regional cerebral blood flow (rCBF) was measured using the 14C-iodoantipyrine autoradiographic technique. Microscopic arterial and venous oxygen saturations were determined using microspectrophotometry. In the cortex contralateral to MCA occlusion, the average rCBF and the average O² consumption were lower in the GYKI Group than in the Control Group (rCBF:GYKI 65.5±24.1, Control 97.7 33.4ml/100g/min;O² consumption: GYKI3.9±1.2, Control 6.2±2.5ml O²/100g/min) without a significant difference in the number of veins with SvO²<50%. In the ischemic cortex, the number of veins with SvO²<50% was significantly smaller in the GYKI Group (21 veins out of 63)than in the Control Group(45 out of 59)without a significant difference in the average rCBF(GYKI44.9±17.7, Control 29.7±10.4) or regional O² consumption between these two groups (GYKI 3.3±1.4,Control 27.7±1.2). Our data demonstrated that GYKI 52466 was effective in improving microscopic O² balance in the focal ischemic cortical area of the brain and it decreased O² consumption in the non-ischemic cortex. [Neurological Research 1999;21:299-304]

Dlg5 Regulates Dendritic Spine Formation and Synaptogenesis by Controlling Subcellular <i>N</i>-Cadherin Localization

Wang, Shih-Hsiu J.,Celic, Ivana,Choi, Se-Young,Riccomagno, Martin,Wang, Qiang,Sun, Lu O.,Mitchell, Sarah P.,Vasioukhin, Valera,Huganir, Richard L.,Kolodkin, Alex L. Society for Neuroscience 2014 The Journal of neuroscience Vol.34 No.38

<P>Most excitatory synapses in the mammalian brain are formed on dendritic spines, and spine density has a profound impact on synaptic transmission, integration, and plasticity. Membrane-associated guanylate kinase (MAGUK) proteins are intracellular scaffolding proteins with well established roles in synapse function. However, whether MAGUK proteins are required for the formation of dendritic spines <I>in vivo</I> is unclear. We isolated a novel <I>disc large-5</I> (<I>Dlg5</I>) allele in mice, <I>Dlg5</I><SUP>LP</SUP>, which harbors a missense mutation in the DLG5 SH3 domain, greatly attenuating its ability to interact with the DLG5 GUK domain. We show here that DLG5 is a MAGUK protein that regulates spine formation, synaptogenesis, and synaptic transmission in cortical neurons. DLG5 regulates synaptogenesis by enhancing the cell surface localization of <I>N</I>-cadherin, revealing a key molecular mechanism for regulating the subcellular localization of this cell adhesion molecule during synaptogenesis.</P>

Analytical Elasto-plastic Solution for Frost Force of Cold-Region Tunnels considering Anisotropic Frost Heave in the Surrounding Rock

Qiang Feng,Shenggang Fu,Chengxiang Wang,Weiwei Liu,Ying Wang,Weiguo Qiao 대한토목학회 2019 KSCE Journal of Civil Engineering Vol.23 No.9

There is an increasing trend of tunnel infrastructure construction in cold regions due to the advance of a western development strategy. To reduce the frost damage sustained by a tunnel, it is important to consider the mechanical properties of frost heave in the surrounding rock by analyzing the anisotropic frost heave of a cold-region tunnel. This paper elaborates on the behavior of anisotropic frost heave in the rock surrounding a cold-region tunnel, and the coefficient of displacement relief is adopted to indirectly quantify the influence of the tunnel construction process. The analytical elasto-plastic solutions of the stress and deformation in the surrounding rock are derived with Drucker-Prager criteria (hereinafter referred to as the D-P criteria) by modifying the constitutive equation for the frozen surrounding rock. Finally, an example is given to obtain the influence law of the distribution of stress and deformation in the surrounding rock. The results indicate that the plastic radius and frost force calculated by considering isotropic frost heave are greater than those obtained by considering anisotropic frost heave. The influence of the related parameters also analyzed. Some useful insights are provided for future numerical simulations and the design and construction of cold-region tunnels.

Mechanical and Microscopic Properties of Copper-contaminated Soil Solidified with Calcium Carbide Residue, Metakaolin, and Desulfurization Gypsum under Freeze-thaw Cycles

Qiang Wang,Dandan Ge,Guojun Cai,Man Li,Liuyan Wu,Huangrui Xu 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.2

The solidification/stabilization effect of heavy metal contaminated soils in frozen soil regions may be weakened. A novel binder comprising calcium carbide residue, metakaolin, and desulfurization gypsum was used to solidify and stabilize copper-contaminated soil subjected to freeze-thaw (F-T) cycles. The unconfined compressive strength (UCS), F-T cycles, scanning electron microscope (SEM), X-ray diffraction (XRD), and pH tests were conducted to investigate the characteristics of UCS, deformation, pH value, and microscopic mechanism of contaminated soils. The results demonstrated that the UCS of contaminated soils decreased with the increasing F-T cycles and Cu2+ concentration; the strength loss rate increased first and then decreased as the F-T cycles increased. The failure strain rose first and then reduced with the increase of F-T cycles, and increased with the rise of Cu2+ concentration. There was a considerable correlation between UCS, failure strain, and deformation modulus E50. The pH value decreased with the increasing F-T cycles and Cu2+ concentration. The microscopic characteristics indicated that the formation of hydrated calcium silicate and ettringite was the fundamental reason for the enhanced UCS. Besides, heavy metals could be solidified and stabilized by chemical precipitation, physical encapsulation, and ion exchange. The effect of F-T cycles could significantly damage the internal structure of contaminated soils, and the frost heaving force could loosen the soil skeleton structure, leading to the deterioration of the mechanical properties.

Adaptive Steering Control Scheme for Combination Vehicles to Track Target Lane

Qiang Wang,Masahiro Oya,Toshihiro Kobayashi 제어로봇시스템학회 2006 제어로봇시스템학회 국제학술대회 논문집 Vol.2006 No.10

Fault-tolerant Topology Control Algorithm for Mobile Robotic Networks

Qiang Wang,Jie Chen,Hao Fang 제어·로봇·시스템학회 2014 International Journal of Control, Automation, and Vol.12 No.3

In multi-agent systems, it is crucial to maintain a robust and fault-tolerant network topology while minimizes power consumption, especially for the multiple unmanned combat platforms based on mobile robotic networks. This work studies the problem of fault-tolerant topology control in mobile robotic networks. With the aim of constructing self-healing networks, a K-connected topology control algorithm that can cope with faults such as node failures and link disruptions is proposed. The robotic team stays connected in the dynamic interaction topology, even in the face of K−1 nodes departure. Our approach combines power transmission and motion control for constructing a K-connected net-work topology with approximately minimum power to prolong their working life. Extensive numerical simulations demonstrate the effectiveness of the proposed solution are presented.

Numerical Investigation on Electromagnetism and Heat Transfer in Electroslag Remelting Process with Triple- Electrode

QIANG WANG,Fengsheng Qi,Fang Wang,Baokuan Li 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.

A transient three-dimensional (3D) model is developed for understanding the electromagnetism, heat transfer and phase change in electroslag remelting (ESR) furnace with triple-electrode. The electromagnetic fields are solved by Maxwell’s equations using the finite element method. The temperature field and phase change are modeled by the enthalpy approach. A reasonable agreement is obtained between experiment and simulation. The electric current flows from one electrode into the slag and comes out from other two electrodes. Most electric current travels through the slag near the slag/ingot interface. A great amount of the Joule heating is generated by the slag and a higher value is found in the slag around the three electrodes. The highest temperature is located at the center of the slag layer. Increase in the current causes the increase in the Joule heating. A hotter slag and a deeper metal pool therefore are formed. The total Joule heating in the slag increases with more slag, while the average Joule heating density reduces. The metal pool depth increases and then decreases with the increasing slag thickness, and the deepest metal pool is obtained at the slag thickness of 200 mm. The power efficiency and the slag temperature reduce with the increasing electrode immersion depth. A shallower electrode immersion depth is a better choice for the ESR process with triple-electrode.

Preliminary Proteomic Analysis of Indomethacin's Effect on Tumor Transplanted with Colorectal Cancer Cell in Nude Mice

Wang, Yu-Jie,Zhang, Gui-Ying,Xiao, Zhi-Qiang,Wang, Hong-Mei,Chen, Zhu-Chu Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.2

Nonsteroidal anti-inflammatory drugs such as indomethacin (IN) can exert anti-colorectal cancer (CRC) activity through cyclooxygenase independent mechanism, but the exactly biological mechanism is not completely known. Here we use proteomic tools to investigate the molecular mechanism of this action. First, nude mice bearing tumors derived from subcutaneous injection with human CRC cell line HCT116 were randomly allocated to groups treated with or without indomethacin. Later, tumor lumps were incised and then total proteins extracted. After separated with two-dimensional electrophoresis, thirty-one differently expressed spots were found between IN-treated and non-IN-treated groups, of which 25 spots decreased and 6 spots increased in abundance in IN-treated group. Through matrix-assisted laser desorption ionization time of flight mass spectrometry and then NCBInr and SWISS-PROT databases searching, 12 protein spots were finally identified including galectin-1, annexin A1, annexin IV, trancription factor BTF3A, calreticulin. Most of the identified proteins are correlated with tumor's biological prosperities of proliferation, invasion, apoptosis and immunity, or take part in cell's signal transduction. From above we thought that indomethacin can exert its effect on colorectal cancer through regulating several proteins' expression directly or indirectly. Further study of these proteins may be helpful in founding new targets of drugs for cancer chemotherapy.

Dilution of Precision in Three Dimensional Angle‑of‑Arrival Positioning Systems

Qiang Wang,Binghao Li,Chris Rizos 대한전기학회 2019 Journal of Electrical Engineering & Technology Vol.14 No.6

Dilution of precision (DOP) for time-of-arrival (TOA) or time-diference-of-arrival (TDOA) positioning systems has been thoroughly investigated. This is not the case for angle-of-arrival (AOA) positioning systems, especially three dimensional AOA systems. However, the recent increase in deployment of antenna arrays and multipath mitigation techniques make AOA systems more attractive. In this paper, the DOPs for position systems using range measurements are reviewed and an expression for 3D AOA DOP is derived. The average values of DOPs for diferent deployments of base station geometries are examined. It is found that for a 3D AOA system, unlike TOA or TDOA systems, vertical DOP (VDOP) is generally smaller than horizontal DOP (HDOP), and the best VDOP can be achieved when the base stations are at the same height as the user. Moving the base stations away from the plane of the user may decrease the HDOP and Geometric DOP (GDOP) slightly, but it also increases the VDOP. For applications that the height of the user changes frequently, the base stations should be deployed with height close to half the height of the area of interest.

Effects of ultrasonic treatment on the gel properties of microbial transglutaminase crosslinked soy, whey and soy–whey proteins

Qiang Cui,Xibo Wang,Guorong Wang,Rui Li,Xiaodan Wang,Shuang Chen,Jingnan Liu,Lianzhou Jiang 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.5

This paper studied the influences of diverse ultrasonic power treatments on the physico-chemical properties of soy–whey mixed protein induced by microbial transglutaminase. Two groups of 15% (m/v) of protein solution-sole protein (as control group) and mixed protein were prepared and processed under different ultrasonic powers for 30 min. After ultrasonic power treatments, gel properties were significantly increased: under 300 W, the gel hardness of mixed protein reached a maximum of 998.9 g, with its water binding capacity scoring a maximum of 87%. According to the analysis of fluorescence emission spectrum, the fluorescence intensity and maximum absorption peak had changed, for different ultrasonic power treatments had exposed more groups. The Fourier Transform Infrared Spectroscopy also suggested that ultrasonic power treatments could change the secondary structure of gel samples. The scanning electron microscope demonstrated that the network structure of mixed protein gel displayed more regular and uniform after ultrasonic treatments.