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Qian, Zhong‐,Ji,Jung, Won‐,Kyo,Kang, Kyong‐,Hwa,Ryu, BoMi,Kim, Se‐,Kwon,Je, Jae‐,Young,Heo, Soo‐,Jin,Oh, Chulhong,Kang, Do‐,Hyung,Park, Won Sun,Choi, Ilȁ Blackwell Publishing Ltd 2012 Journal of phycology Vol.48 No.2
<P>Microalgae are major primary producers of organic matter in aquatic environments through their photosynthetic activities. Fermented microalga (<I>Pavlova lutheri</I> Butcher) preparation (FMP) is the product of yeast fermentation by <I>Hansenula polymorpha</I>. It was tested for the antioxidant activities including lipid peroxidation inhibitory activity, free‐radical‐scavenging activity, inhibition of reactive oxygen species (ROS) on mouse macrophages (RAW264.7 cell), and inhibited myeloperoxidase (MPO) activity in human myeloid cells (HL60). FMP exhibited the highest antioxidant activity on free‐radical scavenging, inhibitory intracellular ROS, and inhibited MPO activity. MTT [3‐(4,5‐dimethyl‐2‐yl)‐2,5‐diphenyltetrazolium bromide] assay showed no cytotoxicity in mouse macrophages (RAW264.7 cell), human myeloid cells (HL60), and human fetal lung fibroblast cell line (MRC‐5). Furthermore, the antioxidative mechanism of FMP was evaluated by protein expression levels of antioxidant enzyme (superoxide dismutase [SOD] and glutathione [GSH]) using Western blot. The results obtained in the present study indicated that FMP is a potential source of natural antioxidant.</P>
A novel sintered metal fiber microfiltration of bio-ethanol fermentation broth
Qian Kang,Jan Baeyens,Tianwei Tan,Raf Dewil 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.8
In bio-ethanol fermentation, the broth consists of mainly water and ethanol, together with particulate residues of unreacted feedstock and additives (mostly yeast). Prior to further processing (distillation), and to avoid fouling of heat exchangers and distillation columns, the solids residues of the broth need to be removed to as low a concentration as possible. The current mechanical separation (belt filter or centrifuge) can only remove +10 μm particles representing about 90% of the total solids content. The remaining 10% is usually recovered in the bottom stream of the first distillation column, and forms the stillage that is further treated. To avoid fouling and even eliminate the first distillation column where the ethanol fraction is only increased from 12% (feed) to 16% (top), a better particulate removal is required. Novel sintered metal fiber (SFM) fleeces are highly efficient for microfiltration, and the removal of suspended solids largely exceeds 99%. The paper (i) positions microfiltration in the overall bio ethanol process; (ii) describes the novel sintered metal fiber microfiltration application; (iii) experimentally determines the major operating characteristics of SFM and (iv) predicts the up-scaled operation by using a simplified filtration model. At an ambient feed temperature, the flux of permeate exceeds 5m3/m2h for a TMP of 1.5 bar and a yeast concentration of 15 g/l, as commonly encountered in the fermenter broth.
Qian, Yongteng,Du, Jimin,Kang, Dae Joon Elsevier 2019 Microporous and mesoporous materials Vol.273 No.-
<P><B>Abstract</B></P> <P>Highly porous nanostructures having large specific surface areas are very desirable for electrochemical supercapacitors to achieve a large energy storage capacity. We synthesized porous Co-doped TiO<SUB>2</SUB> nanostructures with an average diameter of 450 nm through a simple solvothermal method by using tetrabutyl titanate and cobalt acetate as precursors and polystyrene beads as templates. The optimized 7% Co-doped TiO<SUB>2</SUB> nanostructures-based supercapacitor electrodes exhibited a specific capacitance of 352 F g<SUP>-1</SUP> at a current density of 0.5 A g<SUP>−1</SUP> while retaining a capacity of 97.2% after 3000 cycles. This excellent electrochemical performance may be ascribed to the synergistic effects originating from conductivity enhancement of TiO<SUB>2</SUB> through optimized Co-doping and larger specific surface areas rendered by structural porosity, when compared to the undoped TiO<SUB>2</SUB> samples. Our results suggest that porous Co-doped TiO<SUB>2</SUB> nanostructures can be explored for potential electrochemical applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Synthesis of porous Co-doped TiO<SUB>2</SUB> nanostructures by a simple sol–gel method. </LI> <LI> Conductivity enhancement of TiO<SUB>2</SUB> through optimized Co-doping. </LI> <LI> More charge transfer pathways and reaction active sites owing to porosity. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Kang, Kyong-Hwa,Qian, Zhong-Ji,Ryu, BoMi,Karadeniz, Fatih,Kim, Daekyung,Kim, Se-Kwon The Korean Society of Food Science and Nutrition 2013 Preventive Nutrition and Food Science Vol.18 No.2
In this study, novel peptides (NIPP-1, NIPP-2) derived from Navicula incerta (microalgae) protein hydrolysate were explored for their inhibitory effects on collagen release in hepatic fibrosis with the investigation of its underlying mechanism of action. TGF-${\beta}1$ activated fibrosis in LX-2 cells was examined in the presence or absence of purified peptides NIPP-1 and NIPP-2. Besides the mechanisms of liver cell injury, protective effects of NIPP-1 and NIPP-2 were studied to show the protective mechanism against TGF-${\beta}1$ stimulated fibrogenesis. Our results showed that the core protein of NIPP-1 peptide prevented fibril formation of type I collagen, elevated the MMP level and inhibited TIMP production in a dose-dependent manner. The treatment of NIPP-1 and NIPP-2 on TGF-${\beta}1$ induced LX-2 cells alleviated hepatic fibrosis. Moreover, ${\alpha}$-SMA, TIMPs, collagen and PDGF in the NIPP-1 treated groups were significantly decreased. Therefore, it could be suggested that NIPP-1 has potential to be used in anti-fibrosis treatment.
Qian, Yongteng,Yang, Mengke,Zhang, Fangfang,Du, Jimin,Li, Kaidi,Lin, Xialing,Zhu, Xinrui,Lu, Yayun,Wang, Weimin,Kang, Dae Joon Elsevier 2018 Materials characterization Vol.142 No.-
<P><B>Abstract</B></P> <P>It is well known that both catalytic efficiency and stability are the two important parameters of photocatalysts for visible-light-driven hydrogen production reactions. However, light-driven hydrogen evolution based applications still suffer from sluggish reaction kinetics due to the lack of high-performance photocatalysts. In this paper, we successfully synthesized a ternary porous CdS/WO<SUB>3</SUB>/TiO<SUB>2</SUB> photocatalyst with high efficiency and stability via two-stage approach. The as-prepared samples are characterized by XRD, FESEM, EDS, TEM, XPS, and UV–Vis, respectively, which illustrated that the CdS and WO<SUB>3</SUB> moieties are in-situ formed inside the porous TiO<SUB>2</SUB>. Particularly, the photocatalytic hydrogen (H<SUB>2</SUB>) evolution rate of such ternary 8% CdS/WO<SUB>3</SUB>/TiO<SUB>2</SUB> (molar ration of CdS:WO<SUB>3</SUB>:TiO<SUB>2</SUB> = 8:8:100) photocatalyst ranges up to 2106 μmol h<SUP>−1</SUP> g<SUP>−1</SUP> under visible-light irradiation, which is higher than that of pure TiO<SUB>2</SUB> and other binary (CdS/TiO<SUB>2</SUB> and WO<SUB>3</SUB>/TiO<SUB>2</SUB>) porous photocatalysts. The superior H<SUB>2</SUB> evolution efficiency can be attributed to the coexistence of CdS and WO<SUB>3</SUB> in porous TiO<SUB>2</SUB> which can promote the interfacial charge transfer and separation as well as extend the light absorption up to the visible range.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Highly efficient and stable photocatalytic activity of CdS/WO<SUB>3</SUB>/TiO<SUB>2</SUB> photocatalysts were synthesized by a two-step method. </LI> <LI> CdS and WO<SUB>3</SUB> inlaid on porous TiO<SUB>2</SUB> can extend the light absorption and enhance photogenerated electron-hole pairs separation. </LI> <LI> The porous structure can provide more reaction active sites and improve photoproduced-electrons and holes transport speed. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>