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남혜원,현영희,변진원 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.6
This study was performed to develop a standardized recipe for yuza pyun made of citron juice that is by-product of citron tea, to increase the utilization of citron. The effects of mungbean starch(15%, 18%) and dilution ratio of yuza extract(100%, 75%, 50%) on the quality characteristics were evaluated. The quality characteristics of the samples were estimated in terms of sweetness, color difference, texture profile analysis and sensory evaluation. Sweetness of the 18% mungbean groups were higher than 15% groups(p<0.001). The values of Hunter color system indicated that higher content of yuza extract tended to increase yellowness significantly(p<0.001). Texture profiles of hardness, adhesiveness, springiness, cohesiveness, gumminess of yuzapyun showed no significant differences among samples. For sensory evaluation, color and sourness were decreased as yuza extract was diluted, whereas elasticity and sweetness were increased. Yuza pyun containing 18% of mungbean starch with 50% diluted extract was the most preferred in overall acceptability. According to multiple regression analysis, color, sweetness, elasticity and sourness(in order) were important control factors in overall acceptability.
Danielle Ooyoung Pyun,Miri K. Chung,Ho Jung Choi 고려대학교 언어정보연구소 2013 언어정보 Vol.17 No.-
Pyun, Danielle O., Chung, Miri K. & Choi, Ho Jung. 2013. KFL learners’ acquisition of Korean locative particles: A corpus-based contrastive interlanguage analysis. Language Information. Volume 17. 23-46. Adopting the contrastive interlanguage analysis methodology, this study investigates KFL (Korean as a Foreign Language) learners’ acquisition of Korean locative case particles. A learner corpus compiled from eighty-seven intermediate learners of Korean was compared with a native speaker corpus on the frequency distribution of the five semantic categories of the Korean locative case (i.e, static location, dynamic location, time, goal, and source). In addition, learner errors on the use of locative case particles were quantified and examined according to the five semantic categories. The results of this study showed that KFL learners’ use of locative case particles substantially deviated from native speakers’ usage in frequency. Learners’ inaccurate and limited use of locative case particles were attributed to such factors as L1 transfer, overgeneralization, prior input, and interlanguage development.
Pyun, W-B,Hahn, W,Kim, D-S,Yoo, W-S,Lee, S-D,Won, J-H,Rho, B-S,Park, Z-Y,Kim, J-M,Kim, S Macmillan Publishers Limited 2010 Gene Therapy Vol.17 No.12
Hepatocyte growth factor (HGF) has been shown to induce angiogenesis in vivo and has potential as a candidate gene for ‘therapeutic angiogenesis’. In vivo, two isoforms of HGF, HGF<SUB>723</SUB> and HGF<SUB>728</SUB>, consisting of 723 and 728 amino acids, are generated through alternative splicing between exons 4 and 5, but the biological effects of their coexpression have not yet been elucidated. In this study, we generated a series of genomic–complementary DNA (cDNA) hybrids of the HGF gene by inserting various truncated intron 4 into the junction of exons 4 and 5 of HGF cDNA and analyzed the biological activities of these hybrid constructs. We showed that: (1) the hybrid called HGF-X7, which contained 1502 base pairs of intron 4, could drive a higher level of HGF expression than other hybrid constructs and cDNAs of each isoform alone; (2) the pCK vector was most efficient for the gene expression of HGF-X7; (3) coexpression of both isoforms of HGF could more efficiently induce the migration of human umbilical vein endothelial cell (HUVEC) and of the mouse myoblast cell line C<SUB>2</SUB>C<SUB>12</SUB> myoblasts than a single isoform of HGF and human vascular endothelial growth factor (VEGF)<SUB>165</SUB> at a given concentration; (4) intramuscular administration of pCK-HGF-X7 resulted in transient and localized HGF expression in the injected muscle without an increase in the HGF protein levels in other tissues including serum; and (5) intramuscular injection of pCK-HGF-X7 could more efficiently increase the number of angiographically recognizable collateral vessels, as well as improve an intra-arterial Doppler wire-measured blood flow in the rabbit model of hindlimb ischemia when compared with the identical vector encoding VEGF<SUB>165</SUB> gene. These results showed that transfer of the genomic–cDNA hybrid of the HGF gene could be used as a potential therapeutic approach to human vascular diseases.
Pyun Sang Yong,Paik Kyu Cheol,Han Man So,Cho Bong Rae 대한화학회 2021 Bulletin of the Korean Chemical Society Vol.42 No.7
Reactions of 4-nitrophenyl 2-furoates (1a–e) with R2NH/R2NH2 + in 20 mol% DMSO(aq) have been studied. The reactions produced aminolysis products and exhibited second-order kinetics. The Brönsted plots were linear with βnuc values of 0.75–0.89, which remained nearly the same for all 5-furyl substituents. The rate data showed excellent correlations on the Yukawa-Tsuno plots with ρ(x) = 0.72–1.1, and r = 0.55–0.95, respectively. The ρ value increased and r value decreased with a stronger nucleophile, indicating an increase in the electron density at the C O bond and a decrease in the resonance contribution. The results have been interpreted with the addition–elimination mechanism in which the second step is the rds. By comparing with the data for ArC(O)OC6H4-4-NO2 (Ar = Ph, thienyl), the effect of the aryl group on the acyl transfer reaction was assessed.
Pyun, Sang-Yong,Byun, Woong-Sub,Cho, Bong-Rae Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.6
Nitrile-forming eliminations from $(E)-2,4,6-(NO_2)_3C_6H_2CH=NOC_6H_4-2-X-4-NO_2$ (1) promoted by $R_3NH/R_3NH^+$ in 70 mol % MeCN(aq) have been studied kinetically. When X = $NO_2$, the reactions exhibited second-order kinetics as well as Br$\"{o}$nsted ${\beta}$ = 0.63 and ${\mid}{\beta}_{lg}{\mid}$ = 0.34-0.46, and an E2 mechanism is evident. As the leaving group was made poorer (X = H, Cl, and $CF_3$), Br$\"{o}$nsted ${\beta}$ value increased from 0.63 to 0.85-0.89 without much change in the ${\mid}{\beta}_{lg}{\mid}$ value E2, indicating that structure of the transition state changed to an E1cb-like with extensive $C_{\beta}-H$ bond cleavage, significant negative charge development at the ${\beta}$-carbon, and limited $C_{\alpha}$-OAr bond cleavage.
Pyun, Sang-Yong,Cho, Eun-Ju,Seok, Hyoun-Jung,Kim, Ju-Chang,Lee, Seok-Hee,Cho, Bong-Rae Korean Chemical Society 2007 Bulletin of the Korean Chemical Society Vol.28 No.6
Ketene-forming eliminations from C4H3(S)CH2C(O)O-C6H3-2-X-4-NO2 (1) promoted by R2NH in MeCN have been studied kinetically. The reactions are second-order and exhibit Bronsted β =0.51-0.62 and |βlg|= 0.47-0.53. Hence, an E2 mechanism is evident. The Bronsted β increased from 0.33 to 0.53 and |βlg| remained nearly the same by the change of the base-solvent from Bz(i-Pr)NH/Bz(i-Pr)NH2+ in 70 mol% MeCN(aq) to Bz(i-Pr)NH-MeCN, indicating a change to a more symmetrical transition state with similar extents of Cβ -H and Cα -OAr bond cleavage. When the β-aryl group was changed from thienyl to phenyl in MeCN, the β value increased from 0.53 to 0.73 and |βlg| decreased from 0.53 to 0.43. This indicates that the transition state became skewed toward more Cβ -H bond breaking with less Cα-OAr bond cleavage. Noteworthy is the greater double bond stabilizing ability of the thienyl group in the ketene-forming transition state.