DNA Methylation Pattern of Gene Promoters of MB-COMT, DRD2, and NR3C1 in Turkish Patients Diagnosed with Schizophrenia

Hasan Mervan Aytac(Hasan Mervan Aytac ),Yasemin Oyaci(Yasemin Oyaci ),Mustafa Pehlivan(Mustafa Pehlivan ),Sacide Pehlivan(Sacide Pehlivan ) 대한정신약물학회 2022 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.20 No.4

Objective: We aim to evaluate the methylation status of membrane-bound catechol-O-methyltransferase (MB-COMT ) promotor, dopamine receptor D2 (DRD2 ), and nuclear receptor subfamily 3 group C member 1 (NR3C1 ) gene in patients with SCZ by comparing healthy controls. Methods: A sample of 110 patients with SCZ and 100 age- and sex-matched healthy volunteers was included in the study. The interview was started by filling out data forms that included sociodemographic and clinical information. The Structured Clinical Interview for DSM-IV Axis I Disorders was used to confirming the diagnosis according to DSM-IV-TR criteria. Then the patients were evaluated with the Positive and Negative Symptoms Scale in terms of symptom severity. Methylation-specific polymerase chain reaction was used to determine the methylation status of MB-COMT promotor, DRD2 , and NR3C1 gene from DNA material. Results: When we compared the percentages of MB-COMT promotor, DRD2 , and NR3C1 gene methylation status in SCZ patients with the healthy control group, the percentages of MB-COMT promotor (OR: 0.466; 95% CI: 0.268− 0.809; p = 0.006), DRD2 (OR: 0.439; 95% CI: 0.375−0.514; p < 0.001), and NR3C1 (OR: 0.003; 95% CI: 0.001− 0.011; p < 0.001) gene methylation status of SCZ was found to be significantly different from the control group. Whereas unmethylation of MB-COMT promotor and NR3C1 genes were associated with SCZ, the partial methylation of the DRD2 gene was related to the SCZ. Conclusion: The MB-COMT promotor, DRD2 , and NR3C1 gene methylation status may be associated with the SCZ in the Turkish population.

Frequency analysis of GPS data for structural health monitoring observations

Pehlivan, Huseyin Techno-Press 2018 Structural Engineering and Mechanics, An Int'l Jou Vol.66 No.2

In this study, low- and high-frequency structure behaviors were identified and a systematic analysis procedure was proposed using noisy GPS data from a 165-m-high tower in ${\dot{I}}stanbul$, Turkey. The raw GPS data contained long- and short-periodic position changes and noisy signals at different frequencies. To extract the significant results from this complex dataset, the general structure and components of the GPS signal were modeled and analyzed in the time and frequency domains. Uncontrolled jumps and deviations involving the signal in the time domain were pre-filtered. Then, the signal was converted to the frequency domain after applying low- and high-pass filters, and the frequency and periodic component values were calculated. The spectrum of the tower motion obtained from the filtered GPS data had dominant peaks at a low frequency of $1.15572{\times}10-4Hz$ and a high frequency of 0.16624 Hz, consistent with two equivalent GPS datasets. Then, the signal was reconstructed using inverse Fourier transform with the dominant low frequency values to obtain filtered and interpretable clean signals. With the proposed sequence, processing of noisy data collected from the GPS receivers mounted very close to the structure is effective in revealing the basic behaviors and features of buildings.

Effect of shearing on some physiological and hormonal parameters in Akkaraman sheep

Pehlivan, Erkan,Kaliber, Mahmut,Konca, Yusuf,Dellal, Gursel Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.5

Objective: Shearing is one of the practices that is applied periodically to fiber producing animals, which can also alter resistance of animals to high temperatures in especially summer months. This study aimed to investigate effects of shearing on some physiological and hormonal parameters in Akkaraman sheep during summer season. Methods: This study was carried out on 39 non-pregnant Akkaraman ewes (aged 1.5 years at the beginning of experiment). The 39 ewes were chosen randomly from the flock belonging to the Erciyes University and they were assigned to two groups as follows: i) group A (n = 20) designed as the control group, they were shorn and group B (n = 19) designed as the experimental group, they were unshorn. Prior to the shearing (-1 day) and on days 1, 7, 15, 30, 45, 60, 75, and 90 following the shearing, blood samples were taken from the vena jugularis of each sheep. Cortisol, β-endorphin, growth hormone (GH), thyroxine (T₄), triiodothyronine (T₃), and heat shock protein 70 (HSP-70) concentrations were determined using the enzyme immunoassay method. Body weight (BW), rectal temperature (RT), pulse rate (PR), and respiratory rate (RR) of each sheep were recorded at the same time. The data obtained were analyzed using two-way repeated measures analysis of variance. Results: Statistical analysis showed a significant effect of shearing×period interaction (p<0.01) and a significant effect of period (p<0.01) on BW, HSP-70, cortisol, T₄ and RT, PR, GH, β-endorphin, T₃, respectively. Also these analysis showed no significant effect of shearing×period interaction or period on RR. Conclusion: The results showed that the thermoregulation abilities of sheep were affected by shearing treatment and the shorn ewes were less affected by heat stress. In conclusion, based on the data of this study, shearing can be considered as a necessary management practice that requires protection for sheep from the effect of heat stress.

Usability of inclinometers as a complementary measurement tool in structural monitoring

Hüseyin Pehlivan,Halim Ferit Bayata 국제구조공학회 2016 Structural Engineering and Mechanics, An Int'l Jou Vol.58 No.6

In the last few years, many structural monitoring studies have been performed using different techniques to measure structures of different scales such as buildings, dams or bridges. One of the mostly used tools are GPS instruments, which have been utilized in various combinations with accelerometers and some other conventional sensors. In the current study, observation series were recorded for 8 hours with GPS receivers (NovAtel) and Inclination Measurement Sensors mounted on a television tower in Istanbul, Turkey. Each series of observations collected from two different sensors were transformed into a single coordinate system (Local Topocentric Coordinates System). The positional changes of the tower were calculated from the GPS and the inclination data. These changes were plotted in two dimensions (2D) on the same graphic. Thus, the possibility of comparison and analysis were found using the data from both the GPS and the Inclinometer complement each other, in the real test area. The positional changes of the tower were modeled for further examination. As a result, the movement of the tower within an area of 1×1 cm2 was observed. Based on the results, it can be concluded that inclinometers can be used for monitoring the structural behavior of the tower.

Frequency analysis of GPS data for structural health monitoring observations

Hüseyin Pehlivan 국제구조공학회 2018 Structural Engineering and Mechanics, An Int'l Jou Vol.66 No.2

In this study, low- and high-frequency structure behaviors were identified and a systematic analysis procedure was proposed using noisy GPS data from a 165-m-high tower in İstanbul, Turkey. The raw GPS data contained long- and short-periodic position changes and noisy signals at different frequencies. To extract the significant results from this complex dataset, the general structure and components of the GPS signal were modeled and analyzed in the time and frequency domains. Uncontrolled jumps and deviations involving the signal in the time domain were pre-filtered. Then, the signal was converted to the frequency domain after applying low- and high-pass filters, and the frequency and periodic component values were calculated. The spectrum of the tower motion obtained from the filtered GPS data had dominant peaks at a low frequency of 1.15572×10-4 Hz and a high frequency of 0.16624 Hz, consistent with two equivalent GPS datasets. Then, the signal was reconstructed using inverse Fourier transform with the dominant low frequency values to obtain filtered and interpretable clean signals. With the proposed sequence, processing of noisy data collected from the GPS receivers mounted very close to the structure is effective in revealing the basic behaviors and features of buildings.

Identification of structural displacements utilizing concurrent robotic total station and GNSS measurements

Hüseyin Pehlivan 국제구조공학회 2022 Smart Structures and Systems, An International Jou Vol.30 No.4

Monitoring large structures is a significant issue involving public health on which new studies are constantly carried out. Although the Global Navigation Satellite System (GNSS) is the most preferable method for measuring structural displacements, total stations, one of the classical geodetic instruments, are the first devices that come to mind in cases that require complementary usage and auxiliary measurement methods. In this study, the relative displacements of the structural movements of a tower were determined using robotic total stations (RTS) and GNSS. Two GNSS receivers and two RTS observations were carried out simultaneously for 10 hours under normal weather conditions. The spectral analysis of the GNSS data was performed using fast Fourier transform (FFT), and while the dominant modal frequencies were determined, the total station data were balanced with the least-squares technique, and the position and position errors were calculated for each measurement epoch. It has been observed that low-frequency structural movements can be determined by both methods. This result shows that total station measurements are a helpful alternative method for monitoring large structures in situations where measurements are not possible due to the basic handicaps of GNSS or where it is necessary to determine displacements with short observations.

Macular Choroidal Thickness and Volume Measured by Swept-source Optical Coherence Tomography in Healthy Korean Children

Emre Pehlivan,Salih Uzun 대한안과학회 2016 Korean Journal of Ophthalmology Vol.30 No.4

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Effects of TNFalpha, NOS3, MDR1 Gene Polymorphisms on Clinical Parameters, Prognosis and Survival of Multiple Myeloma Cases

Basmaci, C,Pehlivan, M,Tomatir, AG,Sever, T,Okan, V,Yilmaz, M,Oguzkan-Balci, S,Pehlivan, S Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3

It is not clear how gene polymorphisms affecting drugs can contributes totheir efficacy in multiple myeloma (MM). We here aimed to explore associations among gene polymorphisms of tumor necrosis factor alpha (TNFalpha), nitric oxide synthesis 3 (NOS3) and multi-drug resistance 1 (MDR1), clinical parameters, prognosis and survival in MM patients treated with VAD (vincristine-adriamycine-dexamethasone), MP (mephalane-prednisolone), autolougus stem cell transplantation (ASCT), BODEC (bortezomib-dexamethasone-cyclophosphamide) and TD (thalidomide-dexamethasone). We analyzed TNFalpha, NOS 3 and MDR1 in 77 patients with MM and 77 healthy controls. The genotyping was performed with PCR and/or PCR-RFLP. There was no clinically significant difference between MM and control groups when TNFalpha (-238) and (-857) and MDR1 gene polymorphisms were studied. However, the TNFalpha gene polymorphism (-308) GG genotype (p=0.012) and NOS3 (+894) TT genotype (p=0.008) were more common in the MM group compared to healthy controls. NOS3 (VNTR) AA (p=0.007) and NOS3 (+894) GG genotypes (p=0.004) were decreased in the MM group in contrast. In conclusion, the NOS3 (+894) TT and TNFalpha (-308) GG genotypes may have roles in myeloma pathogenesis.

Association of TAP1 and TAP2 Gene Polymorphisms with Hematological Malignancies

Ozbas-Gerceker, Filiz,Bozman, Nazli,Gezici, Sevgi,Pehlivan, Mustafa,Yilmaz, Mehmet,Pehlivan, Sacide,Oguzkan-Balci, Sibel Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Transporter associated with antigen presenting (TAP) 1 and TAP2 genes are localized in the major histocompatability complex (MHC) class II region and form a heterodimer playing a key role in endogenous pathways for antigen presentation. Defects of these genes have been reported to be common in different types of cancer. Polymorphisms identified in these loci have also been investigated and reported to be associated with several autoimmune disorders, viral infections and neoplasms. In the present study, for the first time, the allele and genotype frequencies of TAP1-333, TAP2-565, TAP2-651 and TAP2-665 were determined in patients with hematological malignancies (HM) using a PCR-RFLP method and compared with the frequencies in the control group. Our results suggested an association of TAP1-333 polymorphism with multiple myeloma-MM and TAP2-565 polymorphism with chronic lymphoid leukemia-CLL. In addition, it could be concluded that the TAP2-665 GG genotype might be a risk factor for all types of hematological malignancies included in this study.

Association of an LMP2 Polymorphism with Acute Myeloid Leukemia and Multiple Myeloma

Ozbas-Gerceker, Filiz,Bozman, Nazli,Kok, Selcuk,Pehlivan, Mustafa,Yilmaz, Mehmet,Pehlivan, Sacide,Oguzkan-Balci, Sibel Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Hematological malignancies (HM) are a group of neoplasms derived from the cells of the bone marrow and lymphatic system. Genetic factors leading to susceptibility to HM have been investigated for years but little is known yet. Low molecular weight polypeptide (LMP) 2 and LMP7 genes are important subunits of the immunoproteasome and play significant role in antigen presentation. The polymorphisms of LMP genes have been reported to be risk factors for various types of diseases. The aim of this study was to investigate the association of LMP2 and LMP7 polymorphisms with the occurrence of particular types of HM. A total of 132 patients with HM and 130 control subjects were investigated. No significant difference was obtained in the distribution of genotype and allele frequencies of LMP7 gene in HM patients and the control group. On the other hand, the prevalence of LMP2-AA genotype was found to be higher in acute myeolid leukemia (AML) patients while it was significantly lower in multiple myeloma (MM) cases than in the control subjects. Our results suggested that LMP7 could not be a risk factor for susceptibility to HM, whereas LMP2 polymorphisms could play a role in the development of AML and MM.