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Park, Jong-Lyul,Kim, Seon-Kyu,Kim, Jeong-Hwan,Yun, Seok Joong,Kim, Wun-Jae,Kim, Won Tae,Jeong, Pildu,Kang, Ho Won,Kim, Seon-Young Korea Genome Organization 2018 Genomics & informatics Vol.16 No.3
Because castration-resistant prostate cancer (CRPC) does not respond to androgen deprivation therapy and has a very poor prognosis, it is critical to identify a prognostic indicator for predicting high-risk patients who will develop CRPC. Here, we report a dataset of whole genomes from four pairs of primary prostate cancer (PC) and CRPC samples. The analysis of the paired PC and CRPC samples in the whole-genome data showed that the average number of somatic mutations per patients was 7,927 in CRPC tissues compared with primary PC tissues (range, 1,691 to 21,705). Our whole-genome sequencing data of primary PC and CRPC may be useful for understanding the genomic changes and molecular mechanisms that occur during the progression from PC to CRPC.
Forensic Body Fluid Identification by Analysis of Multiple RNA Markers Using NanoString Technology
Park, Jong-Lyul,Park, Seong-Min,Kim, Jeong-Hwan,Lee, Han-Chul,Lee, Seung-Hwan,Woo, Kwang-Man,Kim, Seon-Young Korea Genome Organization 2013 Genomics & informatics Vol.11 No.4
RNA analysis has become a reliable method of body fluid identification for forensic use. Previously, we developed a combination of four multiplex quantitative PCR (qRT-PCR) probes to discriminate four different body fluids (blood, semen, saliva, and vaginal secretion). While those makers successfully identified most body fluid samples, there were some cases of false positive and negative identification. To improve the accuracy of the identification further, we tried to use multiple markers per body fluid and adopted the NanoString nCounter system instead of a multiplex qRT-PCR system. After measuring tens of RNA markers, we evaluated the accuracy of each marker for body fluid identification. For body fluids, such as blood and semen, each body fluid-specific marker was accurate enough for perfect identification. However, for saliva and vaginal secretion, no single marker was perfect. Thus, we designed a logistic regression model with multiple markers for saliva and vaginal secretion and achieved almost perfect identification. In conclusion, the NanoString nCounter is an efficient platform for measuring multiple RNA markers per body fluid and will be useful for forensic RNA analysis.
Biological safety and B cells activation effects of Zanthoxylum schinifolium
Park, Dae-Hun,Park, Yong-Il,Li, Yong-Chun,Xu, Hong De,Lee, Jong-Hwa,Kim, Young-Jin,Jang, Soon-Ae,Park, Sung-Jin,Lee, Yun-Lyul,Ahn, Jae-Bum,Cho, Soon-Chang,Lee, Min-Jae 대한독성유전단백체학회 2011 Molecular & cellular toxicology Vol.7 No.2
Zanthoxylum schinifolium has been used as culinary applications in Asia and some of its elements have been elucidated to have various pharmaceutical effects such as anti-platelet aggregation, inhibitory activities against monoamine oxidase, antioxidant and anticancer activities, and anti-inflammatory activity. In this study 21-day repeated Zanthoxylum schinifolium oral administration (250 mg/kg/day, once a day) modulated B cell-related immunity and biologically safe. After 21-day repeated oral administration, the expression of CD19 bound cells was significantly increased compared to those of the other surface molecules and the percentages of Alamar $Blue^{(R)}$ reduction were in LPS-treated splenocytes higher than in no treated but it was not different with in Con A-treated splenocytes and in no treated. Twenty-one-day repeated Z. schinifolium administration could not change the index of biological safety such as CBC, hematological enzymes, and especially histopathological morphologies. From the results Z. schinifolium-repeated administration enhances B cell immunity and is biological safe. Conclusively Z. schinifolium is one of the safe candidates to modulate immunity.
Cell-Free miR-27a, a Potential Diagnostic and Prognostic Biomarker for Gastric Cancer
Park, Jong-Lyul,Kim, Mirang,Song, Kyu-Sang,Kim, Seon-Young,Kim, Yong Sung Korea Genome Organization 2015 Genomics & informatics Vol.13 No.3
MicroRNAs (miRNAs) have been demonstrated to play an important role in carcinogenesis. Previous studies revealed that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. In this study, we measured the plasma expression levels of three miRNAs (miR-21, miR-27a, and miR-155) to investigate the usefulness of miRNAs for gastric cancer detection. We initially examined plasma miRNA expression levels in a screening cohort consisting of 15 patients with gastric cancer and 15 healthy controls from Korean population, using TaqMan quantitative real-time polymerase chain reaction. We observed that the expression level of miR-27a was significantly higher in patients with gastric cancer than in healthy controls, whereas the miR-21 and miR-155a expression levels were not significantly higher in the patients with gastric cancer. Therefore, we further validated the miR-27a expression level in 73 paired gastric cancer tissues and in a validation plasma cohort from 35 patients with gastric cancer and 35 healthy controls. In both the gastric cancer tissues and the validation plasma cohort, the miR-27a expression levels were significantly higher in patients with gastric cancer. Receiver-operator characteristic (ROC) analysis of the validation cohort, revealed an area under the ROC curve value of 0.70 with 75% sensitivity and 56% specificity in discriminating gastric cancer. Thus, the miR-27a expression level in plasma could be a useful biomarker for the diagnosis and/or prognosis of gastric cancer.
박종률(Jong Lyul Park) 한국스포츠교육학회 2003 한국스포츠교육학회지 Vol.10 No.1
이 연구의 목적은 경쟁 스포츠 참여시 스포츠맨십 발현 실태에 대해 교육적 문화 기술 방법을 통해 심층적으로 분석하는 것이다. 이러한 연구 목적을 달성하기 위해 충청북도 청원군 소재의 실업계 고등학교 1학년 4명을 참여 관찰 대상자로 삼았으며,2001년 1학기 수업 기간을 집중 자료 수집 기간으로 선정하였다. 체육 수업 중 경쟁성을 극대화하기 위해 축구와 농구 종목을 대상으로 개작된 스포츠 교육 모형을 적용하였으며 참여관찰과 심층면담, 기록물 검토, VCR 촬영 등을 통해 자료를 수집하였다. 수집된 자료는 질적 연구의 자료 분석 방법인 사례기록과 귀납적 범주 분석을 통해 분석하였으며 삼각검증법을 통해 자료의 신뢰성을 추구하였다. 수집된 자료를 분석 및 해석한 결과 경쟁 스포츠 참여시 고등학생의 스포츠맨십 발현 실태는 언어습관과 스포츠맨십 행동 양식으로 구분되어졌다. 참여 관찰 대상자들은 경쟁 스포츠 참여시 승리 지상주의에 지나치게 매몰되어 부정적 언어 습관을 나타내고 있었으며, 스포츠맨십 행동에 대한 의미와 가치,필요성에 대한 인식이 부재하였으며,자신의 이익과 관심의 추구에 매진하는 등 이기성에 기초한 자기 중심적 행동 특성을 나타냈다. The purpose of this study is to analyse how one recognizes sportsmanship while participating in highly competitive sports games through the method of educational enthnography. For this study, I chose four highschool first-year students from a vocational highschool located in Chungbuk as subjects. I decided to collect needed data intensively during the first semester in 2001. To intensify the competition, the applied sports teaching model for soccer and basketball was used. The data were collected by intensive interviews, observation, analysis for hand writings and VCR tapes. The collected data were analyzed by case recording and Inductive Category Analysis Method, and the credibility for the data was guaranteed by the Triangulation Along with the results of the analysis and interpretation of the collected data, I could discover that highschool students` Aspect of sportsmanship in competitive sport games can be divided into two aspects: their habits of language use and behavioral patterns of sportsmanship. The subjects showed very habits of negative language use due to their devotion for victory in a very competitive game. As a result, they did not seem to be aware of the significance, value, and meaning of genuine sportsmanship. Besides, they also showed certain egocentric behavioral characteristics, in which they just pursued their own benefits and interests based on egocentricity.