<i>In vitro</i> inhibitory effects of Wen‐pi‐tang‐Hab‐Wu‐ling‐san on human cytochrome P450 isoforms

Lee, H. W.,Kim, D. W.,Phapale, P. B.,Lim, M. ‐,S.,Park, J.,Seo, J. J.,Park, K. M.,Park, Y. ‐,K.,Yoon, Y. ‐,R. Blackwell Publishing Ltd 2011 Journal of clinical pharmacy and therapeutics Vol.36 No.4

<P><B>Summary</B></P><P><B>What is known and Objective: </B> Although Wen‐pi‐tang‐Hab‐Wu‐ling‐san (WHW), an oriental herbal medicine, has been prescribed for the treatment of chronic renal failure (CRF) in Korean clinics, no studies regarding WHW–drug interactions had been reported. The purpose of this study was to evaluate the possibility that WHW inhibits the catalytic activities of major cytochrome P450 (CYP) isoforms.</P><P><B>Methods: </B> The abilities of various WHW extracts to inhibit phenacetin O‐de‐ethylation (CYP1A2), tolbutamide 4‐methylhydroxylation (CYP2C9), omeprazole 4′‐hydroxylation (CYP2C19), dextromethorphan O‐demethylation (CYP2D6), chlorzoxazone 6‐hydroxylation (CYP2E1) and midazolam 1‐hydroxylation (CYP3A4) were assessed using human liver microsomes.</P><P><B>Results and Discussion: </B> WHW extract at concentrations up to 100 μ<SMALL>m</SMALL> showed negligible inhibition of the six CYP isoforms tested (CYP1A2, CYP2C9, CYP2C19, CYP2D6, CYP2E1 and CYP3A4), with apparent IC<SUB>50</SUB> values (concentration of the inhibitor causing 50% inhibition of the original enzyme activity) of 817.5, 601.6, 521.7, 310.2, 342.8 and 487.0 μg/mL, respectively.</P><P><B>What is new and Conclusion: </B> Our <I>in vitro</I> findings suggest that WHW extract at concentrations corresponding to a clinically recommended dosage range has no notable inhibitory effects on CYP isoforms. Therefore, we believe that WHW extract may be free of drug–herb interactions when co‐administered with other medicines. However, <I>in vivo</I> human studies are needed to confirm these results.</P>

Variable number of tandem repeats of 9 Plasmodium vivax genes among Southeast Asian isolates

Wang, B.,Nyunt, M.H.,Yun, S.G.,Lu, F.,Cheng, Y.,Han, J.H.,Ha, K.S.,Park, W.S.,Hong, S.H.,Lim, C.S.,Cao, J.,Sattabongkot, J.,Kyaw, M.P.,Cui, L.,Han, E.T. Verlag fur Recht und Gesellschaft 2017 Acta tropica Vol.170 No.-

<P>The variable number of tandem repeats (VNTRs) provides valuable information about both the functional and evolutionary aspects of genetic diversity. Comparative analysis of 3 Plasmodium falciparum genomes has shown that more than 9% of its open reading frames (ORFs) harbor VNTRs. Although microsatellites and VNTR genes of P. vivax were reported, the VNTR polymorphism of genes has not been examined widely. In this study, 230 P. vivax genes were analyzed for VNTRs by SERV, and 33 kinds of TR deletions or insertions from 29P. vivax genes (12.6%) were found. Of these, 9 VNTR fragments from 8 P. vivax genes were used for PCR amplification and sequence analysis to examine the genetic diversity among 134 isolates from four Southeast Asian countries (China, Republic of Korea, Thailand, and Myanmar) with different malaria endemicity. We confirmed the existence of extensive polymorphism of VNTR fragments in field isolates. This detection provides several suitable markers for analysis of the molecular epidemiology of P. vivax field isolates. (C) 2017 Elsevier B.V. All rights reserved.</P>

국내이용 말사료의 영양적 가치와 YEAST CULTURE 첨가시 소화율 , 광물질 이용율 및 혈액성상에 미치는 영향

김성민(S . M . Kim),김종민(C . M . Kim),이현기(H . K . Lee),박원표(W . P . Park),임영재(Y . J . Lim),김병진(B . J . Kim),정태영(T . Y . Chung) 한국영양사료학회 1991 韓國營養飼料學會誌 Vol.15 No.5

Effect of polymorphisms in the GBP1, Mx1 and CD163 genes on host responses to PRRSV infection in pigs

Niu, P.,Shabir, N.,Khatun, A.,Seo, B.J.,Gu, S.,Lee, S.M.,Lim, S.K.,Kim, K.S.,Kim, W.I. Elsevier Scientific Pub. Co 2016 Veterinary microbiology Vol.182 No.-

<P>Porcine reproductive and respiratory syndrome (PRRS) is the most economically important disease to the swine industry, and effective prevention strategy for this disease is still required. Guanylate-binding protein 1 (GBP1) and myxovirus resistance protein 1 (Mx1) are two important proteins belonging to the GTPase superfamily that have been previously described to show antiviral effects. CD163 is considered the most important receptor for PRRSV attachment and internalization. Therefore, the aim of the present study was to evaluate the effects of these genes on host resistance against PRRSV infection in conjunction with the host immune response following PRRSV challenge. The results showed that pigs with AG genotype for the GBPI exon2 exhibited a significantly higher average daily weight gain (ADWG) and lower average viremia than AA or GG genotype. Furthermore, pigs harbouring the AG genotype for the GBP1 gene presented greater CD4(+)CD25(+) and CD8(+)CD25(+) T cell populations at 4 and 18 days post challenge (dpc), respectively, as compared with other genotypes whereas pigs with CC genotype for the CD163 gene displayed significantly higher nucleocapsid-specific antibody titers at 11 dpc. However, pigs with a single 11-bp deletion or insertion in the Mx1 gene did not show significant differences in either weight gain or viremia. Based on these results, we concluded that GBPI is most significantly associated with resistance against PRRSV infection and efficient T cell activation in pigs. (C) 2015 Elsevier B.V. All rights reserved.</P>

Portable, non-destructive tester integrating VIS/NIR reflectance spectroscopy for the detection of sugar content in Asian pears

Choi, J.H.,Chen, P.A.,Lee, B.,Yim, S.H.,Kim, M.S.,Bae, Y.S.,Lim, D.C.,Seo, H.J. International Society for Horticultural Science ; 2017 Scientia horticulturae Vol.220 No.-

<P>In this study, a portable, non-destructive tester, integrating near-infrared (NIR) spectroscopy, was developed to determine the maturity of Asian pears. In addition, regression analysis was conducted for mature Manpungbae pears using the partial least squares regression model with the Brix value of Asian pears in a wavelength range of 710-930 nm, and the standard error of calibration or standard error of prediction (SEC/SEP) of 0.41-0.43 degrees Brix and relative standard error of calibration or relative standard error of prediction (rSEC/rSEP) = 0.91-0.92 were observed, indicating excellent prediction performance with no dependence on pretreatment conditions. Furthermore, the reliability of the developed Brix determination model was confirmed using Manpungbae, Wonhwang, Chuhwangbae, and Niitaka pears, and SEP, rSEP, and bias values of 0.29-0.33 degrees Brix, 0.90-0.96, and 1.67E-04, respectively, were observed, indicating high reliability. Hence, the construction of a Brix determination model by the accumulation of absorbance for several varieties of pears in each harvest period is expected to determine optimum harvest times via the measurement of the Brix values at full maturity regardless, of the variety. (C) 2017 Elsevier B.V. All rights reserved.</P>

Analysis of Si wafer coated by spin-on-glass for laser ablation ICP-MS

Ryu, W. K.,Kim, J. S.,Lee, J. S.,Lim, H. B.,Jun, P. K. Royal Society of Chemistry 2007 Journal of analytical atomic spectrometry Vol.22 No.6

<P>A method involving laser ablation sector type inductively coupled plasma mass spectrometry (LA-ICP-MS) was developed for the direct determination of metal impurities in the spin-on-glass (SOG) layer on a silicon wafer surface. For a reference wafer, the preparation procedure for the SOG layer was re-designed and optimized in a sequence of spiking of known amounts of metal contaminants in SOG chemical (Model: P-MSQ, 5.5% silicon dioxide in polypylene and glycol–dimethyl ether), spin-coating, and baking. Optimization of the procedure affected the contamination levels of metals which were determined by a solution scanning sampling followed by a sector type ICP-MS analysis. For homogeneity study, 74 sectors of the SOG layer were individually scanned using a laboratory-made automatic scanner with a scanning solution. The average contamination levels of each sector were obtained in the range of 10<SUP>10</SUP>–10<SUP>12</SUP> atoms cm<SUP>−2</SUP> under optimized conditions. SEM images of craters for LA, each produced by one laser shot (266 nm, 9 mJ per pulse), showed that the depths of the craters were larger than the thickness of the SOG layer, 2350 Å, and the diameter increased with the power and decreased with de-focused beam diameter. Limits of detection of LA-ICP-MS were estimated at ∼10<SUP>9</SUP> atoms cm<SUP>−2</SUP> on the Si wafer surface, depending on the element.</P> <P>Graphic Abstract</P><P>A laser ablation-ICP-MS method was developed for direct determination of metal impurities in the SOG layer on a silicon wafer surface and preparation of a reference wafer. The detection limit was ∼10<SUP>9</SUP> atoms cm<SUP>−2</SUP>. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b618184h'> </P>

Reproduction and dispersal pattern of egg parasitoids of Riptortus pedestris (Hemiptera: Alydidae) after installing pheromone traps plus non-viable host eggs

Mainali, B.P.,Lim, U.T. 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol. No.

<P>The aggregation pheromone of Riptortus pedestris (Fabricius) (Hemiptera: Alydidae) has a kairomonal effect on its egg parasitoids. The addition of non-viable R. pedestris eggs to aggregation pheromone traps was suggested as a means to enhance reproduction of parasitoids attracted to pheromone traps and was found to enhance field parasitism. In this study, we tested if the eggs added to traps are indeed used by parasitoids for their reproduction. Spatial distributions of egg parasitism were assessed in a barley and a sesame field where non-viable host eggs were installed at four distances (8,16, 24, and 32 m) in two directions from the aggregation pheromone traps placed in the center of each field. While field parasitism increased significantly over time, it decreased with distance from traps. Higher egg parasitism was found up to 16 m from the point of release in both barley and sesame fields. To compare temporal changes in parasitism, traps were also installed in another barley and another sesame field without adding host eggs. From regression slope comparisons, significant differences were found only in the sesame fields, where the parasitism rate on the last sampling date in the egg-released field was higher than the comparison field by 80%. A higher male proportion was found near the trap installation point only for Gryon japonicum (Ashmead) (Hymenoptera: Platygastridae) in the sesame field. These findings suggest that supplemental host eggs in traps enhance field parasitism by increasing the reproduction and dispersion of field populations of parasitoids. (C) 2015 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.</P>

Benserazide, the first allosteric inhibitor of Coxsackievirus B3 3C protease

Kim, B.K.,Cho, J.H.,Jeong, P.,Lee, Y.,Lim, J.J.,Park, K.R.,Eom, S.H.,Kim, Y.C. North-Holland Pub ; Elsevier Science Ltd 2015 FEBS letters Vol.589 No.15

Coxsackievirus B3 is the main cause of human viral myocarditis and cardiomyopathy. Virally encoded Coxsackievirus 3C protease (3C<SUP>pro</SUP>) plays an essential role in viral proliferation. Here, benserazide was discovered as a novel inhibitor from a drug library screen targeting Coxsackievirus 3C<SUP>pro</SUP> using a FRET-based enzyme assay. Benserazide, whose chemical structure has no electrophilic functional groups, was characterized as a non-competitive inhibitor by enzyme kinetic studies. A molecular docking study with benserazide and its analogs indicated that a novel putative allosteric binding site was involved. Specifically, a 2,3,4-trihydroxybenzyl moiety was determined to be a key pharmacophore for the enzyme's inhibitory activity. We suggest that the putative allosteric binding site may be a novel target for future therapeutic strategies.

Characterization of Nutritional Value for Twenty-one Pork Muscles

Kim, J.H.,Seong, P.N.,Cho, S.H.,Park, B.Y.,Hah, K.H.,Yu, L. H.,Lim, D.G.,Hwang, I.H.,Kim, D.H.,Lee, J.M.,Ahn, C.N. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.1

A study was conducted to evaluate nutritional value for twenty-one pork muscles. Ten market-weight crossbred pigs (five gilts and five barrows) were used for evaluating proximate chemical composition, cholesterol, total iron, calorie and fatty acid contents. As preliminary analysis revealed no noticeable sex effect, pooled data from both sexes were used for the final analysis. M. rectus femoris had the highest moisture content, while m. latissimus dorsi was lowest in moisture content (p<0.05). Protein content was highest for m. longissimus dorsi and lowest for m. supraspinatus (p<0.05). The tensor fasciae and latissimus dorsi muscles contained the highest intramuscular fat (p<0.05), while rectus femoris, adductor and vastus lateralis were lowest in intramuscular fat content. When simple correlations between chemical values were computed for the pooled dataset from all muscles, intramuscular fat had significant (p<0.05) negative linear relationships with moisture (r = -0.85) and protein (r = -0.51) contents. Calorie levels were not significantly affected by fat content, while rectus femoris and latissimus dorsi muscles showed lowest and highest calorie contents, respectively (p<0.05). Polyunsaturated fatty acid content was highest (p<0.05) for both m. adductor and m. rectus femoris, while it was lowest for m. longissimus dorsi. Collectively, the current study identified a large amount of variation in nutritional characteristics between pork muscles, and the data can be used for the development of muscle-specific strategies to improve eating quality of meats and meat products.

차세대 리소그래피 빛샘 발생을 위한 플라스마 집속 장치의 제작과 아르곤 아크 플라스마의 발생에 따른 회로 분석 및 전기 광학적 특성 연구

이수범,문민욱,오필용,송기백,임정은,홍영준,이원주,최은하,Lee S.B.,Moon M.W.,Oh P.Y.,Song K.B.,Lim J.E.,Hong Y.J.,Yi W.J.,Choi E.H. 한국진공학회 2006 Applied Science and Convergence Technology Vol.15 No.4

본 연구에서는 극자외선 (Extreme Ultra Violet) 리소그래피의 빛샘원 발생을 위한 플라스 마 집속장치 (Plasma Focus Device)를 설계, 제작하였으며, 이를 이용하여 단펄스 집속 플라스마의 전류, 전압 방전 특성 및 장비의 저항, 인덕턴스의 중요 기초 연구를 수행하였다. 전압, 전류는 C-dot probe 와 B-dot probe를 이용하여 측정하였다. Anode 전극에 1.5, 2, 2.5, 3 kV의 전압을 인가하고 Diode chamber 내의 Ar 기체압력을 1 mTorr-100 Torr 로 변화시켰을 때 발생되는 전압, 전류는 300 mTorr 에서 가장 큰 값을 보였으며, 이때 측정된 LC 공진에 의한 전류 파형으로부터 계산된 시스템 내의 인덕턴스와 임피던스값은 각각 73 nH, $35 m{\Omega}$ 였다. 300 mTorr, 2.5 kV 일 때 Emission spectroscopy를 이용하여 계산한 단펄스 집속 Ar 플라스마내의 전자온도는 Local Thermodynamic Equilibrium(LTE) 가정으로부터 T=13600 K 이었고 이온밀도 및 이온화율은 각각 $N_i = 8.25{\times}10^{15}/ cc,\;{\delta}= 77.8%$ 이었다. In this study, we had designed and fabricated the plasma focus device which can generate the light source for EUV(Extreme Ultra Violet) lithography. And we also have investigated the basic electrical characteristics of currents, voltages, resistance and inductance of this system. Voltage and current signals were measured by C-dot and B-dot probe, respectively. We applied various voltages of 1.5, 2, 2.5 and 3 kV to the anode electrode and observed voltages and current signals in accordance with various Ar pressures of 1 mTorr to 100 Torr in diode chamber. It is observed that the peak values of voltage and current signals were measured at 300 mTorr, where the inductance and impedance were also estimated to be 73 nH and $35 m{\Omega}$ respectively. The electron temperature has been shown to be 13000 K at the diode voltage of 2.5 kV and this gas pressure of 300 mTorr. It is also found that the ion density Ni and ionization rate 0 have been shown to be $N_i = 8.25{\times}10^{15}/cc$ and ${\delta}$= 77.8%, respectively by optical emission spectroscopy from assumption of local thermodynamic equilibrium(LTE) plasma.