In vivo evidence on the functional variation within fatty acid synthase gene associated with lipid metabolism in bovine longissimus dorsi muscle tissue

Oh, Dong-yep,Nam, Insik,Hwang, Sehwan,Kong, Hongsik,Lee, Honggu,Ha, Jaejung,Baik, Myunggi,Oh, Man Hwan,Kim, Songmi,Han, Kyudong,Lee, Yoonseok 한국유전학회 2018 Genes & Genomics Vol.40 No.3

<P>In Korean cattle, intramuscular fat (IMF), or marbling, of the longissimus dorsi muscle (LM) cross section is one of the most important indicators of beef quality and are influenced by environmental and genetic factors. This study was to evaluate the effect of SNPs on the beef quality in Korean cattle for functional studies, such as site-directed mutagenesis based on bovine adipocytes. The fatty acid synthase (FASN) gene plays an important role in lipogenesis. FASN is an essential metabolic and multifunctional enzyme in fatty acid synthesis. Several studies have reported that SNPs g.841G, g.16024A, g.16039T, and g.17924G have a significant impact on marbling scores in Korean cattle and Japanese Black cattle population. These SNPs are located in transcription factor binding sites, the beta-ketoacyl reductase, and thioesterase domains. Our results revealed that the g.17924 A > G SNP is located in the thioesterase domain of the FASN protein, and changes from polar, neutral, and hydrophilic to nonpolar, aliphatic, and hydrophobic, respectively. In in vivo LM tissue of Korean cattle, the g.17924A > G SNP has an effect on increasing fat deposition. Therefore, g.17924A > G SNP could be a causal mutation for increasing fat deposition in Korean cattle LM tissue.</P>

The DNA replication-related element (DRE)-DRE-binding Factor (DREF) system may be involved in the expression of the Drosophila melanogaster TBP gene

Choi, Tae-Yeong,Cho, Nam-Young,Oh, Younsang,Yoo, Mi-Ae,Matsukage, Akio,Ryu, Yoonseok,Han, Kyuhyung,Yoon, Jaeseung,Baek, Kwanghee 경희대학교 생명자원과학연구원 2000 硏究論文集 Vol.21 No.-

The TATA box binding protein (TBP) is a general transcription factor required for initiation by all three eukaryotic RNA polymerases. Previously, we found that the promoter region of the Drosophila melanogaster TBP gene contains three sequences similar to the DNA replication-related element (DRE) (5-TATCGATA). In the present study, we found that the DRE-like sequences are also present in the promoter of the Drosophila virilis TBP gene, suggesting a role for these sequences in TBP expression. Band mobihty shift assays revealed that oligonucleotides containing sequences similar to the DRE of D. melanogaster TBP gene promoter form specific complexes with a factor in a Kc cell nuclear extract and with recombinant DRE-binding factor (DREF). Furthermore, these complexes were either supershifted or diminished by monoclonal antibodies to DREF. Transient luciferase assays demonstrated that induction of mutations in two DRE-related sequences at positions -223 and -63 resulted in an extensile reduction of promoter activity. Thus, the DRE-DREF system appears to be involved in the expression of the D. melanogaster TBP gene. ⓒ 2000 Federation of European Biochemcal Societies. Published by Elsevier Science B.V. All rights reserved.

호모그래피기반의 카메라 추적기술을 이용한 텔레프레즌스 시스템

김태협(Taehyub Kim),최윤석(Yoonseok Choi),남보담(Bodam Nam),홍현기(Hyunki Hong) 大韓電子工學會 2012 電子工學會論文誌-CI (Computer and Information) Vol.49 No.3

텔레프레즌스(tele-presence)와 원격조정(tele-operation) 기술은 멀리 떨어진 사용자에게 몰입감이 높은(immersive) 장면이나 모바일 로봇 등의 제어 환경을 제공한다. 본 논문에서는 호모그래피(homography) 정보 기반의 카메라 추적기술을 이용한 텔레프레즌스 시스템이 제안된다. 먼저 카메라가 탑재된 HMD(head mounted display)를 착용한 사용자의 머리 움직임을 카메라 추적기술로 분석한다. 그리고 전방향(omni-directional) 카메라를 장착한 로봇으로부터 입력되는 파노라마 영상에서 사용자의 시야(field of view)에 해당하는 장면을 생성하여 HMD를 통해 디스플레이한다. 사용자의 움직임을 추정하는 과정에서 3차원 평면으로 구성된 공간의 호모그래피 정보를 이용하며, 실제로 측정된 3차원 데이터를 기준으로 마커기반의 ARToolkit을 이용하는 방법과 호모그래피 기반 방법의 정확도를 각각 비교하였다. Tele-presence and tele-operation techniques are used to build up an immersive scene and control environment for the distant user. This paper presents a novel tele-presence system using the camera tracking based on planar homography. In the first step, the user wears the HMD(head mounted display) with the camera and his/her head motion is estimated. From the panoramic image by the omni-directional camera mounted on the mobile robot, a viewing image by the user is generated and displayed through HMD. The homography of 3D plane with markers is used to obtain the head motion of the user. For the performance evaluation, the camera tracking results by ARToolkit and the homography based method are compared with the really measured positions of the camera.

Liquid crystal modulator를 이용한 엑스선 검출기에 관한 연구

허승욱(Seunguk Heo),조성호(Sungho Cho),김윤석(Yoonseok Kim),김영빈(Youngbin Kim),남상희(Sanghee Nam),박지군(Jigoon Park) 한국방사선학회 2009 한국방사선학회 논문지 Vol.3 No.1

디지털 방사선 검출기의 주된 연구동향은 영상의 해상도 향상, 대면적화, 동영상 구현 등… 이라 할 수 있다. 이런 연구는 방사선 변환과정에 따라 크게 직접변환 방식과 간접 변환 방식으로 나눌 수 있으며 각각 고유한 장단점을 내포하고 있다. 간접변환 방식의 경우 형광체의 사용으로 인한 Light scattering 문제로 해상력의 저하 그리고 직접방식의 경우는 낮은 system 안정성과 동영상 구현의 어려움 등이 대표적이라 할 수 있다. 이런 문제를 해결하기 위한 연구로써 액정을 이용한 엑스선 검출기 연구가 진행되었으나 그 구조의 특성상 균일한 액정의 주입이 어려워 제작에 있어 많은 어려움이 있다. 따라서 본 논문에서는 기존의 문제점을 해결할 수 있는 구조를 제안하고 이를 Simulation 통해 제안된 구조의 가능성을 검토해 보았다. The trend of Digital x-ray Detector research is to improve resolution of image and to embody large area imaging as well as dynamic moving imaging, etc. This research is divided with indirect conversion method and direct conversion method by radiation conversion process. Each conversion method has problems such as decrease of resolution from light scattering in indirect method case and not only low system stability but also difficult in dynamic moving imaging in direct method case. X-ray detector using liquid crystal has been researching to solve these problems, but it is difficulty in uniform injection of liquid crystal because of its structural properties. Therefore, this study suggests the structure which solves present problem. Also the possibility of suggested structure was investigated using simulation.

FR-ATM 망 연동에서의 FR 호 수락 제어 연구

오형근(HyungGeun Oh),이성창(Sungchang Lee),남윤석(Yoonseok Nam),김정식(Jungsik Kim) 대한전자공학회 1998 대한전자공학회 학술대회 Vol.1998 No.4

Prediction of canine ovulation using serum estradiol concentration

Zhao Minghui,Lee Seunghoon,No Jingu,Nam Yoonseok,Jeong Hae-yun,Ock Sun A,Yun JeongHee,Kim Dong-Hoon,Tai-Young 한국수정란이식학회 2017 한국수정란이식학회 학술대회 Vol.2017 No.05

Canine cloning have been succeeded for a decade. To obtain in vivo matured dog oocytes, Serum progesterone (P4) level were employed for ovulate determination. However, accuracy of P4 methods is not satisfied. The aim of this study was to compare both methods of serum estradiol (E2) and P4 on the accuracy of canine ovulation determination. Canine serum P4 and E2 concentration during both proestrus and estrus were detected. Correlation between accuracy of each method and environment temperature were analyzed. Following ovulation, oocytes were collected by surgery. As a result, higher percentage of mature oocytes was obtained when using E2 (56.43%) as compared to P4 (39.60%). Accuracy of P4 increased from spring (30.76%) to summer (47.92%) and decreased in autumn (37.50%) and winter (29.16%) gradually. Especially, E2 maintained about 50% to 65% whatever the season and temperature. Correlation analyze showed that dynamic of P4 accuracy highly correlated with environment temperate (Rp4=0.862) but E2 could not be affected by the temperature (RE2=0.199). To determine whether obtained oocytes by E2 method could be used for canine cloning, twenty canines were selected as oocyte donors, and two puppies were produced after somatic cell nuclear transfer(SCNT) and embryo transfer(ET) with the oocytes by E2 method. In conclusion, comparing to the P4 method, the E2 is an accuracy and reliable method for canine cloning.

Decision Making Method Based on Importance-Dangerousness Analysis for the Potential Risk Behavior of Construction Laborers

Han, In-Hye,Yang, Gi-Nam,Kim, Sangyong,Kim, Gwang-Hee,Shin, Yoonseok Hindawi Limited 2015 Mathematical problems in engineering Vol.2015 No.-

<P>Unsafe behavior contributes to 90% of the causes of construction accidents. To prevent construction accidents, studies on existing unsafe behaviors have been regularly conducted. However, existing studies generally tend to average the survey results and conduct analyses thereon, and such a method cannot consider the potential risk as regards people’s anxiety about a certain unsafe behavior. Thus, this research suggests an Importance-Dangerousness Analysis (IDA) technique so that potential risks due to unsafe behaviors of laborers working in the construction sector could be evaluated. In order to verify the applicability of the suggested technique, an actual survey was conducted, and the results of Importance-Performance Analysis (IPA) and IDA were compared with each other. It was found that, unlike IPA, unsafe behaviors that could pose potential risks were confirmed by IDA. Further, unsafe behaviors in the construction sector that should be urgently addressed were also studied. Finally, the IDA suggested in this research could contribute to effective construction safety management on-site by supporting the decisions of the safety manager based on the unsafe behavior analysis of construction laborers.</P>

Dog cloning with in vivo matured oocytes obtained using electric chemilumiescence immunoassay-predicted ovulation method

Lee Seunghoon,Zhao Minghui,No Jingu,Nam Yoonseok,Jeong Hae-yun,Ock Sun A,Yun JeongHee,Kim Dong-Hoon,Hur Tai-Young 한국수정란이식학회 2017 한국수정란이식학회 학술대회 Vol.2017 No.05

To obtain in vivo matured oocytes for dog cloning, serum progesterone (P4) level were employed for ovulate determination. Radioactive immunoassay (RIA) is a traditional serum hormone assay method with highly radioactivity. The aim of this study was to evaluate the reliability of RIA and to compare its canine serum P4 concentration determination accuracy to that of the electric chemiluminescence immunoassay (ECLI). To obtain in vivo matured oocytes for canine somatic cell nuclear transfer, serum P4 levels were accurately measured with both methods of RIA and ECLI. Although both methods detected similar P4 level before ovulation, the mean P4 concentration using ECLI was significantly higher than that using RIA from 3days before ovulation. Following ovulation, oocytes were collected by surgery, and a lower percentage of mature oocytes were observed using ECLI (39%) as compared to RIA (67%) if 4-8ng/ml of P4 were criteria for determination of ovulation. On other hand, high percentage of mature oocytes was observed using ECLI when 6–15 ng/mL of progesterone was criteria for ovulation determination. To determine whether in vivo oocytes obtained by ECLI method could be used for canine cloning, six canines were selected as oocyte donors and two puppies were produced after SCNT and embryo transfer. In conclusion, compared to the traditional RIA method, the ECLI method is a safe and reliable method for canine cloning.

IBB 기반 적응적 컴패니언 스크린 컨텐츠 제공 플랫폼 설계 및 구현

문재원(JaeWon Moon),임태범(Tae-Beom Lim),금승우(SeungWoo Kum),남윤석(YoonSeok Nam),이상원(Sangwon Lee) 한국방송·미디어공학회 2017 방송공학회논문지 Vol.22 No.1

Dog cloning with <i>in vivo</i> matured oocytes obtaining using serum estradiol levels for predicting time of ovulation

Zhao, Minghui,Lee, Seunghoon,Kim, Dong-Hoon,No, Jingu,Nam, Yoonseok,Ock, Sun A.,Ko, Yeoung-Gyu,Hur, Tai-Young Elsevier 2018 Theriogenology Vol.107 No.-

<P><B>Abstract</B></P> <P>Dog cloning using <I>in vivo</I>-matured oocytes has been carried out for a decade. To obtain mature oocytes, serum progesterone (P<SUB>4</SUB>) levels are used to evaluate ovulation. However, the accuracy of these methods is not sufficient. Thus, the aim of the present study was to verify the feasibility of serum estradiol (E<SUB>2</SUB>) on canine ovulation determination as assessed by the percentage of dogs yielding mature oocytes. <I>In vivo</I>-matured oocytes were utilized for canine somatic cell nuclear transfer (SCNT), and serum P<SUB>4</SUB> and E<SUB>2</SUB> levels were assessed to determine ovulation and oocyte maturation. Canine serum P<SUB>4</SUB> and E<SUB>2</SUB> concentrations during both pro-estrus and estrus were analyzed by electrochemiluminescence immunoassay. The percentage of dogs yielding mature oocytes using each of the two ovulation prediction methods were compared, and correlations between the percentage of each method and temperature were analyzed. Following evaluation, oocytes were collected surgically, and a significantly higher percentage (<I>P</I> < 0.05) of dogs yielding mature oocytes was observed using E<SUB>2</SUB> (56.43%) for ovulation detection as compared with that using P<SUB>4</SUB> (39.60%). The percentage of dogs yielding mature oocytes using P<SUB>4</SUB> significantly lower (<I>P</I> < 0.05) than E<SUB>2</SUB> in autumn (P<SUB>4</SUB>, 37.50% vs. E<SUB>2</SUB>, 52.00%) and winter (P<SUB>4</SUB>, 29.17% vs. E<SUB>2</SUB>, 59.09%). Using E<SUB>2</SUB>, the percentage was maintained at about 52.00–66.67% regardless of the season and temperature. Correlation analysis showed that the dynamic of percentage of dogs yielding mature oocyte using P<SUB>4</SUB> was highly correlated with environmental temperature (R<SUB>P4</SUB> = 0.862), whereas E<SUB>2</SUB> was not affected by temperature (R<SUB>E2</SUB> = 0.199). To determine whether serum E<SUB>2</SUB> could be used for ovulation prediction for canine cloning, ovulation of 25 and 19 dogs (<I>P</I> < 0.05) were predicted using P<SUB>4</SUB> or E<SUB>2</SUB> methods, respectively and two puppies, one from each ovulation prediction method, were obtained after SCNT and embryo transfer. Thus, compared with the P<SUB>4</SUB> method, E<SUB>2</SUB> was an accurate and reliable method for canine cloning.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The percentage of dogs yielding mature oocytes following prediction of ovulation using serum P<SUB>4</SUB> was affected by temperature. </LI> <LI> The percentage of dogs yielding mature oocytes following estimation of ovulation with serum E<SUB>2</SUB> was higher than that of dogs with oocytes recovered after the use of P<SUB>4</SUB> for ovulation determination. </LI> <LI> Cloned pups were produced when serum E<SUB>2</SUB> was used for predicting ovulation time. </LI> </UL> </P>