Ethephon 수상처리가 참다래 과실의 숙기 및 품질에 미치는 영향

나양기,조혜성,임경호,김월수 전남대학교 농업과학기술연구소 2000 農業科學技術硏究 Vol.35 No.-

This experiment was carried out to investigate the effects of overhead irrigation of Ethrel on after-ripening of kiwi fruit, and to improve marketability and decrease operating cost as to ripen kiwi fruit at the stage of consumption. In 1994, the eight-year-old Hayward which were planted in farm field at Haenam Okchen were treated with 100ppm, 150ppm and 200ppm overhead irrigation of ethephon on September 25, September 30 and October 5 respectively. In 1995, the nine-year-old Hayward which were planted in windbreak net house at Moonlae Haenam were treated with 40, 70 and 100ppm overhead irrigation of Ethephon on October 5, October 10 and October 15 respectively. As the result of at 10 and 20 days after treatment, in 1994, the firmness was much low as to show 0.8 to 0.95kg at 10 days after treatment, 0.2 to 0.33kg at 20 days after treatment. And sduble solid content was 10.6 to 11.7˚ Bx and abscission rate was 10.3 to 35.9%. Therefore, the marketability were tended to be showed low. In 1995, as that firmness was 3.2 to 3.7kg, last soluble solid content after harvesting was 13.8 to 14.0˚ Bx and abscission rate was 0%, there was no problems in marketability and marketing. Therefore, this results were demonstrated that the treatment after October 10 in 70 to 100ppm ethephon can be improved marketability and marketing of kiwifruit.

진주시 수돗물 불소농도조정사업의 11년간 비용편익 분석

김만경 ( Man Kyong Kim ),정지인 ( Ji In Jung ),김민지 ( Min Ji Kim ),전은주 ( Eun Joo Jun ),김한나 ( Han Na Kim ),김세연 ( Se Yeon Kim ),한동헌 ( Dong Hun Han ),정승화 ( Seung Hwa Jeong ),김진범 ( Jin Bom Kim ) 대한예방치과·구강보건학회 2014 大韓口腔保健學會誌 Vol.38 No.2

Objectives: The aim of this study was to estimate the economic costs and benefits of a water fluoridation program in the city of Jinju, Korea. Methods: In 2009, dental surveys were conducted on 2,315 children aged 6-12 years in Jinju, which had been fluoridated for 11 years. The decayed/missing/filled teeth (DMFT) scores of children in Jinju were compared to the DMFT scores of same-aged children in non-fluoridated small- and mediumsized cities in the fourth Korean National Health and Nutrition Examination Survey conducted from 2007 to 2009 by the Korea Centers for Disease Control and Prevention. The reduced number of decayed permanent teeth by the water fluoridation program was estimated as the difference between the DMFT scores of children in Jinju and those in non-fluoridated small- and medium-sized cities. The economic benefits were estimated by the savings from reduced dental treatment costs by using the reduced number of decayed permanent teeth from the water fluoridation program. All annual costs and benefits were calculated from 1998 to 2009. The social rate and untreated rate of decayed teeth were applied as 3% and 20%, respectively. The annual benefit-cost ratio was estimated by using the annual benefits and cumulative program costs. Results: The economic benefit in 2009 from a water fluoridation program was estimated as 57,496,000,000 Korean Won (KRW), and the cumulative cost in 2009 was estimated as 1,387,000,000 KRW. The net present value in 2009 from a water fluoridation program was 56,109,000,000 KRW. The benefit-cost ratio in 2009 was estimated as 41.4. Conclusions: The economic benefit of a water fluoridation program in Jinju city was evaluated as excellent.

대퇴 골두 골괴사에서 괴사 범위 측정 방법의 비교 분석

강준순 ( Joon Soon Kang ),문경호 ( Kyong Ho Moon ),김려섭 ( Ryuh Sup Kim ),나석인 ( Suk In Na ) 대한고관절학회 2011 Hip and Pelvis Vol.23 No.4

목적: 대퇴 골두 괴사의 예후는 괴사의 크기가 가장 중요한 예후 인자이지만, 괴사의 크기나 범위를 측정하는 방법이 저자마다 차이가 있다. 본 연구는 대퇴 골두 괴사 범위를 측정하는 3가지 방법에 대해 정확도와 상호 연관성을 비교 분석하였다. 대상 및 방법: 대퇴 골두 괴사로 진단된 60 고관절을 대상으로 하였다. 자기 공명 영상 사진에서 괴사 크기를 Steinberg, Kim, 수정된 Kerboul 방법으로 두 명의 측정자가 3회 반복 측정하였다. Steinberg 방법은 자기 공명 영상에서 전산 프로그램을 이용하여 괴사의 실제 면적을 측정하여 15% 미만을 1군(소형 크기 괴사), 15~30%는 2군(중형 크기 괴사), 30% 이상을 3군(대형 크기 괴사)로 분류한 한 후 각 군에 해당하는 다른 두 측정 방법을 대입하여 통계적으로 유의한 값을 산출하였다. 결과: Steinberg 방법에 의한 1군(16예)의 평균 괴사크기는 10.92%, 2군(33예)은 평균 괴사 크기는 21.68%, 3군(11예)의 평균 괴사크기는 36.80% 이었다. Steinberg 방법에 의한 분류를 통계적으로 가장 유의한 수준으로 분석한 결과 Kim 방법은 18% 미만은 1군, 33% 2군, 33% 이상은 3군에 분류되었다. 또한 수정된 Kerboul 방법의 경우는 200.6°미만은 1군, 262.3°2군, 262.4°이상은 3군으로 분류되었다. 결론: Kim 방법과 수정된 Kerboul 방법의 새로운 분류 기준은 대퇴 골두 괴사 치료 방법의 결정 및 예후 판단에 있어 임상적인 연구가 추가로 시행된다면 유용한 지표로 사용될 수 있을 것으로 사료된다. Purpose: The size of the necrotic lesion is known to be the most important prognostic factor in osteonecrosis of the femoral head (ONFH). We evaluated the accuracy and relationship of three different measuring methods of necrotic lesions for ONFH. Materials and Methods: Sixty hips that had ONFH were measured on an MRI by two orthopaedic surgeons using Steinberg, Kim, and modified Kerboul methods. Based on the lesion size of the necrosis as measured with the computerized Steinberg method, the hips were divided into Group I (small lesion: less than 15%), Group II (medium lesion: 15~30%), and Group III (large lesion: more than 30%). Data of the Kim and modified Kerboul methods were reclassified by statistical analysis according to the groups classified by the Steinberg method. Results: Average lesion size of Group I (16 hips) was 10.92%, the average size of Group II (33 hips) was 21.68%, and the average size of Group III (11 hips) was 36.80%. We established a new criteria of the Kim and modified Kerboul methods based on Steinberg. The Kim method was reclassified into Groups I (less than 18%), II (18~33%), and III (33% or more). And the modified Kerboul method was divided into Groups I (less than 200.6°), II (200.6~262.4°), and III (more than 262.4°) as well. Conclusion: New criteria for the Kim and modified Kerboul method would be a useful indicator for the prognosis and treatment plan in ONFH.

Incidence, Morbidity, and Mortality of Achalasia: A Nationwide, Population-Based Cohort Study in South Korea

Kim Ga Hee,Park Hyungchul,Jung Kee Wook,Kim Min-Ju,Kim Ye-Jee,Lee Ji Min,Lee Bong Eun,Min Yang Won,Kim Jeong Hwan,Na Hee Kyong,Ahn Ji Yong,Lee Jeong Hoon,Kim Do Hoon,Choi Kee Don,Song Ho June,Lee Gin 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.6

Background/Aims: Although an association between achalasia and esophageal cancer has been reported, whether achalasia confers a substantial increase in mortality is unknown. Moreover, the causes of death related to achalasia have not been investigated. We performed this nationwide, population-based cohort study on achalasia because no such study has been performed since the introduction of high-resolution manometry in 2008. Methods: This study was performed using data extracted from the Korean National Health Insurance Service database, covering a 9-year period from 2009 to 2017. Control participants without a diagnostic code for achalasia were randomly selected and matched by sex and birth year at a case-to-control ratio of 1:4. Data on the cause of death from Statistics Korea were also analyzed. Results: The overall incidence of achalasia was 0.68 per 100,000 person-years, and the prevalence was 6.46 per 100,000 population. Patients with achalasia (n=3,063) had significantly higher adjusted hazard ratio (aHR) for esophageal cancer (aHR, 3.40; 95% confidence interval [CI], 1.25 to 9.22; p=0.017), pneumonia (aHR, 2.30; 95% CI, 1.89 to 2.81; p<0.001), aspiration pneumonia (aHR, 3.92; 95% CI, 2.38 to 6.48; p<0.001), and mortality (aHR, 1.68; 95% CI, 1.44 to 1.94; p<0.001). Esophageal cancer carried the highest mortality risk (aHR, 8.82; 95% CI, 2.35 to 33.16; p=0.001), while pneumonia had the highest non-cancer mortality risk (aHR, 2.28; 95% CI, 1.31 to 3.96; p=0.004). Conclusions: In this nationwide study, achalasia was associated with increased risk of mortality. Esophageal cancer and pneumonia were the most common comorbidities and the major causes of death in patients with achalasia.

Biomimetic fog harvesting surface by photo-induced micro-patterning of zinc-oxide silver hierarchical nanostructures

Kyong Kim, Na,Hee Kang, Dong,Eom, Hyeonjin,Wook Kang, Hyun Elsevier 2019 APPLIED SURFACE SCIENCE - Vol.470 No.-

<P><B>Abstract</B></P> <P>As water scarcity has become a major global problem, fog-harvesting technologies are considered an effective sustainable solution for water resources. Here, we report a novel approach to the fog-harvesting technology using zinc oxide-silver hierarchical nanostructures to mimic the <I>Stenocara</I> beetle’s back. Vertically aligned zinc oxide nanowires are first fabricated by a cost-effective and scalable hydrothermal method to produce a superhydrophilic surface. Silver nanoparticles are then selectively synthesized by an additional photo-induced synthetic process on the zinc oxide nanowire surfaces to form a hydrophobic surface using the hierarchical nanostructures. The fog-harvesting performance was investigated using an artificial fog flow and by measuring the amount of harvested water for efficient fog harvesting. On the superhydrophilic surface, although the water droplets immediately were captured, they formed a puddle at the bottom of the surface due to the high adhesion between water and the surface. In contrast, on the hydrophobic surface, the capturing rate was very low even though the water droplets easily rolled off the surface. Compared to the non-patterned surface, the captured water film on the patterned hydrophilic region grew rapidly into a spherical shape and separated from the surface due to the surrounding hydrophobic regions. As a result, the patterned surface with 0.5 mm pattern size afforded a higher fog collection rate of 1233 mg/h than those of the superhydrophilic and hydrophobic surfaces of 1105 mg/h and 879 mg/h respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Facile, low-cost, and easy to operate method for the fabrication of mimetic <I>Stenocara</I> beetle’s back. </LI> <LI> Controlling a Surface wettability by the silver nanoparticles on the zinc oxide nanowires. </LI> <LI> Selectively synthesizing the silver nanoparticles and zinc oxide nanowires hierarchical nanostructure. </LI> <LI> Efficient water collection by hydrophobic surface with patterned hydrophilic regions. </LI> <LI> Application to multifunctional surface for water collection and desalination. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

3D cell printing of islet-laden pancreatic tissue-derived extracellular matrix bioink constructs for enhancing pancreatic functions

Kim, Jaewook,Shim, In Kyong,Hwang, Dong Gyu,Lee, Yu Na,Kim, Myungji,Kim, Hyeonji,Kim, Seok-Won,Lee, Song,Kim, Song Cheol,Cho, Dong-Woo,Jang, Jinah The Royal Society of Chemistry 2019 Journal of materials chemistry. B, Materials for b Vol.7 No.10

<P>Type 1 diabetes mellitus (T1DM) is a form of diabetes that inhibits or halts insulin production in the pancreas. Although various therapeutic options are applied in clinical settings, not all patients are treatable with such methods due to the instability of the T1DM or the unawareness of hypoglycemia. Islet transplantation using a tissue engineering-based approach may mark a clinical significance, but finding ways to increase the function of islets in 3D constructs is a major challenge. In this study, we suggest pancreatic tissue-derived extracellular matrix as a potential candidate to recapitulate the native microenvironment in transplantable 3D pancreatic tissues. Notably, insulin secretion and the maturation of insulin-producing cells derived from human pluripotent stem cells were highly up-regulated when cultured in pdECM bioink. In addition, co-culture with human umbilical vein-derived endothelial cells decreased the central necrosis of islets under 3D culture conditions. Through the convergence of 3D cell printing technology, we validated the possibility of fabricating 3D constructs of a therapeutically applicable transplant size that can potentially be an allogeneic source of islets, such as patient-induced pluripotent stem cell-derived insulin-producing cells.</P>

ATP Measurement Predicts Porcine Islet Transplantation Outcome in Nude Mice

Kim, Jae Hyeon,Park, Sang Gyu,Lee, Han Na,Lee, Yeon Yi,Park, Ho Sun,Kim, Hyong-Il,Yu, Jae Eun,Kim, Sun Hee,Park, Chung-Gyu,Ha, Jongwon,Kim, Sang Jun,Park, Kyong Soo Lippincott Williams Wilkins, Inc. 2009 Transplantation Vol.87 No.2

Current nude mice islet transplantation studies cannot be used prospectively. Therefore, to predict transplantation outcomes, reliable and rapid assays for islet quality assessment are warranted. This study evaluated the predictive power of the porcine islet ATP content on the outcomes of islet transplantation in nude mice. Here, we report that the ATP measurement using a small number of handpicked islets with a diameter of 100 to 150 μm is a good predictor of islet graft efficacy in nude mice. Using receiver-operator characteristic analysis, the area under the curve of the ATP content using a small number of handpicked islets was 0.867 (95% confidence interval 0.744–0.989, P<0.001). The sensitivity and the specificity measured were 83.3% and 73.3%, respectively. In conclusion, a simple and a rapid measurement of intraislet ATP content could be a promising substitute for current nude mice islet transplantation studies.

Attenuation of PERK enhances glucose-stimulated insulin secretion in islets

Kim, Min Joo,Min, Se Hee,Shin, Seon Young,Kim, Mi Na,Lee, Hakmo,Jang, Jin Young,Kim, Sun-Whe,Park, Kyong Soo,Jung, Hye Seung Journal of Endocrinology, Ltd. [etc.] 2018 The Journal of endocrinology Vol. No.

<P>PERK is a pancreatic endoplasmic reticulum (ER) kinase. Its complete deletion in pancreatic beta cells induces insulin deficiency; however, the effects of partial Perk suppression are unclear. We investigated the effect of partial PERK suppression using the specific PERK inhibitors GSK2606414 and GSK2656157. Low-dose GSK2606414 treatment for 24 h enhanced glucose-stimulated insulin secretion (GSIS), islet insulin content and calcium transit in mouse (at 40 nM) and human (at 50-100 nM) pancreatic islets. GSK2606414 also induced the expression of the ER chaperone BiP and the release of calcium from the ER. When Bip expression was inhibited using a Bip siRNA, the GSK2606414-induced augmentation of the ER calcium level, islet insulin contents, glucose-stimulated cytosolic calcium transit and GSIS were abrogated. In both wild-type and insulin-deficient Atg7-knockout mice, 8 weeks of GSK2656157 treatment enhanced GSIS and improved hyperglycemia without affecting body weight. In conclusion, partial PERK inhibition induced BiP expression in islets, increased glucose-stimulated calcium transit and islet insulin contents and enhanced GSIS, suggesting that low-dose PERK inhibitors could potentially be used to treat insulin deficiency.</P>

Brazilin Isolated from Caesalpinia sappan suppresses nuclear envelope reassembly by inhibiting barrier-to-autointegration factor phosphorylation.

Kim, Seong-Hoon,Lyu, Ha-Na,Kim, Ye Seul,Jeon, Yong Hyun,Kim, Wanil,Kim, Sangjune,Lim, Jong-Kwan,Lee, Ho Won,Baek, Nam-In,Choi, Kwan-Yong,Lee, Jaetae,Kim, Kyong-Tai American Society for Pharmacology and Experimental 2015 The Journal of Pharmacology and Experimental Thera Vol.352 No.1

<P>To date, many anticancer drugs have been developed by directly or indirectly targeting microtubules, which are involved in cell division. Although this approach has yielded many anticancer drugs, these drugs produce undesirable side effects. An alternative strategy is needed, and targeting mitotic exit may be one alternative approach. Localization of phosphorylated barrier-to-autointegration factor (BAF) to the chromosomal core region is essential for nuclear envelope compartment relocalization. In this study, we isolated brazilin from Caesalpinia sappan Leguminosae and demonstrated that it inhibited BAF phosphorylation in vitro and in vivo. Moreover, we demonstrated direct binding between brazilin and BAF. The inhibition of BAF phosphorylation induced abnormal nuclear envelope reassembly and cell death, indicating that perturbation of nuclear envelope reassembly could be a novel approach to anticancer therapy. We propose that brazilin isolated from C. sappan may be a new anticancer drug candidate that induces cell death by inhibiting vaccinia-related kinase 1-mediated BAF phosphorylation.</P>

Obtusilactone B from Machilus Thunbergii targets barrier-to-autointegration factor to treat cancer.

Kim, Wanil,Lyu, Ha-Na,Kwon, Hyun-Sook,Kim, Ye Seul,Lee, Kyung-Ha,Kim, Do-Yeon,Chakraborty, Goutam,Choi, Kwan Yong,Yoon, Ho Sup,Kim, Kyong-Tai American Society for Pharmacology and Experimental 2013 Molecular pharmacology Vol.83 No.2

<P>Targeting specific molecules is a promising cancer treatment because certain types of cancer cells are dependent on specific oncogenes. This strategy led to the development of therapeutics that use monoclonal antibodies or small-molecule inhibitors. However, the continued development of novel molecular targeting inhibitors is required to target the various oncogenes associated with the diverse types and stages of cancer. Obtusilactone B is a butanolide derivative purified from Machilus thunbergii. In this study, we show that obtusilactone B functions as a small-molecule inhibitor that causes abnormal nuclear envelope dynamics and inhibits growth by suppressing vaccinia-related kinase 1 (VRK1)-mediated phosphorylation of barrier-to-autointegration factor (BAF). BAF is important in maintaining lamin integrity, which is closely associated with diseases that include cancer. Specific binding of obtusilactone B to BAF suppressed VRK1-mediated BAF phosphorylation and the subsequent dissociation of the nuclear envelope from DNA that allows cells to progress through the cell cycle. Obtusilactone B potently induced tumor cell death in vitro, indicating that specific targeting of BAF to block cell cycle progression can be an effective anticancer strategy. Our results demonstrate that targeting a major constituent of the nuclear envelope may be a novel and promising alternative approach to cancer treatment.</P>