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송순기,금동호,오재근,이명종 동국대학교 한의학연구소 1998 東國韓醫學硏究所論文集 Vol.7 No.1
스포츠 과학 분야에서는 운동 선수들의 경기력 향상과 운동 피로의 신속한 회복을 도모하기 위하여 한약을 복용하고 그 효과와 유의성에 대하여 연구가 활발히 이루어지고 있다. 격심한 운동을 수행할 때 나타나는 Glucose 등 에너지원의 고갈, Lactate 등 대사 산물의 축적, 전해질의 불균형 등의 여러 제반 증상이 氣虛로 인하여 나타나는 전신의 증후와 유사하다고 생각하여 補中益氣湯을 투여한 후 인체의 대사 변화를 관찰함으로서 운동수행능력에 미치는 영향과 유의성을 평가하고자 하였다. 본 연구는 장거리 달리기 선수를 대상으로 2주간의 補中益氣湯의 투여가 에너지 대사 및 전해질대사에 미치는 영향을 규명하기 위하여 최대심박수의 70% 강도의 운동을 실시한 후 운동전 안정시, 운동 직후, 휴식 후 10분, 휴식 후 30분, 휴식 후 1시간으로 나누어 혈액검사를 하였을 때 나타나는 각종 변인의 반응 양상을 비교 분석하여 유의한 결과를 얻었다. 혈중 에너지 대사에서는 free fatty acid와 Lactate 반응에서 유의성 있는 차이가 나타났고, 전해질대사에서도 Na^(+), Cl-와 K^(+) 반응에서 모누 유의성 있는 차이를 나타냈다. 그러므로 補中益氣湯은 에너지 및 전해질 대사에서 유의성있는 변화를 나타내었으며 운동수행능력의 향상에 유효하였다. Objective This experimental study was designed to investigate effects of Bo-joong-ik-gi-tang administration among long distance runners on changes of the energy and electrolyte metabolism. Materials and Methods All subjects were divided randomly with two groups, Bo-joong-ik-gi-tang Group (N=4) and control group (N=4) and performed to run the 400m track with 70% of HR max about 1 hour. The blood samples were collected from antecubital vein by 5㎖ syringes at before exercise, immediateIy after exercise, recovering- 10 min, recovering-30 min, recovering-1 hour. These sampie were used to analyze for the factors of the changes on metabolic responses. Firest, the primary factors on the changes of the energy metabolism were checked ; Glucose, Free fatty acid, Lactate, LDH. Second, the primary factors on the changes of the electroIyte metabolisrn were checked ; Na^(+), Cl^(-), K^(+). Results 1. The change of the energy metabolism 1) Glucose response was not shown significant difference between two groups. 2) Free fatty acid response in Bo-joong-ik-gi-tang group was significantly increased at recover-10 min. 3) Lactate response in Bo-joong-ik-gi-tang group was significantly decreased at immediately after exercise, recover-10 min, recover-30 min. 4) LDH response was not shown significant difference between two groups. 2. The change of the electrolyte metabolism 1) Na^(+) response in Bo-joong-ik-gi-tang group was shown significant difference between two groups at before exercise. 2) C^(-) response in Bo-joong-ik-gi-tang group was significantly increased at before exercise, immediately after exercise. 3) K^(+) response in Bo-joong-ik-gi-tang group was significantly increased at recover-10 min. Conclusion According to the above results, it was shown that Bo-joong-ik-gi-tang had the positive effects on changes of the energy and electrolyte metabolism for the long distance runners.
Myeong-Geun Oh,Yong-Hee Kim,Polash Chandra Karmakar,Sang-Eun Jung,Seok-Man Kim,Ju-Hee Jin,Buom-Yong Ryu 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
Spermatogonial stem cells (SSCs) are the basis of spermatogenesis in male. Among the testicular germ cells, SSCs are considered as an important population because of their capability to multiply in numbers which is also known as self-renewal, and to differentiate into spermatocytes by subsequent meiotic processes finalized with the production of spermatozoa. As SSCs are present in a very small proportion among the total testis cells, in vitro proliferation of SSCs in undifferentiated state is very important in the field of experiments related to germ cell culture. The objective of the study was to establish an effective feeder cell for murine germ cell development in vitro. We used three types of feeder cells to grow murine germ cells, such as STO, tissue specific feeder layer, and C166 feeder cells and green fluorescence protein (GFP) rat SSCs were used to grow on them. A continuous culture was performed for each feeder groups, but C166 feeder cells found unable to proliferate germ cells even after several weeks. We observed a comparatively better proliferation rate of rat germ cells grown on tissue specific feeder layer where cultured germ cell colony and cell count were higher than STO feeder. Immunocytochemistry of undifferentiated SSC marker showed significantly higher expression for germ cell cultured on tissue specific feeder layer. Similarly, mRNA level of undifferentiated spermatogonia were found higher in case of tissue specific feeder layer and STO, although the expression level of differentiated spermatogonia was similar. Finally, the functional characteristics of SSCs were studied by cultured germ cell transplantation into recipient mice. We used donor rat germ cells cultured on both feeder groups for 4 months, where SSCs from tissue specific feeder layer showed higher functional and stemness activity. Transplantation of SSCs cultured for 8 months from tissue specific feeder layer group were performed to recipient rat and we could produce GFP offspring from those recipients. Thus the experiment conclude that tissue specific cells could be an effective feeder layer for murine SSCs culture based on their survivability and proliferation along with retaining the perfect functional characteristics and stemness properties.
방어구조물 형상에 따른 토석류의 유입특성과 위험도 평가
오승명 ( Seung Myeong Oh ),송창근 ( Chang Geun Song ),이승오 ( Seung Oh Lee ) 한국안전학회(구 한국산업안전학회) 2016 한국안전학회지 Vol.31 No.6
This study analyzed the inflow characteristics of debris flow according to shape of defensive structure and computed risk index. In order to simulate debris flow, two shapes of defensive structure were considered. Initial mass distribution was set with a rectangular shape and defensive structures were set semi-circular shape and rectangular shape, respectively. It was found that a defensive structure with semicircular shape was more vulnerable to debris impact compared with rectangular shape because the flow mass became concentrated in quadrant part of the inner circle. If the velocity of the debris flow was less than 1 m/s, the risk assessment by FII (Flood Intensity Index) was much appropriate. However, when the movement of debris runout was faster than 1 m/s, the risk index of FHR (Flood Hazard Rating) provided improved classification due to its subdivided hazardous range.
Oh, So-Young,Park, Myeong-Soo,Lee, Yeong-Geun,Thi, Nhan Nguyen,Baek, Nam-In,Ji, Geun-Eog CHAPMAN AND HALL 2019 GLYCOCONJUGATE JOURNAL Vol.36 No.3
<P> Breast-fed infants have <I>Bifidobacterium</I>-rich gut microbiota compared to infants fed formula. Fucosylated oligosaccharides are the major components of human milk oligosaccharide (HMO) which confer various beneficial effects including prebiotic effect and protection from pathogenic infection on the host. A novel prebiotics was developed using bifidobacterial β-galactosidase and fucose and lactose as substrates. Structure analysis revealed it as β-D-galactopyranosyl-(1 → 3)-O-L-fucopyranose named as β-galactosyl fucose (gal-fuc), which is different from common fucosylated HMOs with α1-2, α1-3, and α1-4 linkages. Among the four <I>Lactobacillus</I> strains examined, all but <I>L. delbrueckii</I> subsp. bilgaricus KCTC 3635 grew better on gal-fuc than on β-GOS. Among the 11 bifidobacterial species examined, all except for <I>B. bifium</I> used gal-fuc as much as GOS. Moreover, the gal-fuc was noticeably better used by <I>Bifidobacterium infantis,</I> the major intestinal bacteria of breast fed infant. Among 15 non-probiotic bacteria, only 4 strains used gal-fuc better than β-GOS. In conclusion, a novel gal-fuc is expected to contribute to beneficial changes of gut microbiota. </P>
Oh, So Young,Youn, So Youn,Park, Myeong Soo,Baek, Nam In,Ji, Geun Eog American Chemical Society 2018 Journal of agricultural and food chemistry Vol.66 No.5
<P>The prebiotic effects of GOS (galactooligosaccharides) are known to depend on the glycosidic linkages, degree of polymerization (DP), and the monosaccharide composition. In this study, a novel form of α-GOS with a potentially improved prebiotic effect was synthesized using bifidobacterial α-galactosidase (<I>α-</I>Gal) purified from recombinant <I>Escherichia coli</I>. The carbohydrate produced was identified as α-<SMALL>d</SMALL>-galactopyranosyl-(1→6)-O-α-<SMALL>d</SMALL>-glucopyranosyl-(1→2)-[α-<SMALL>d</SMALL>-galactopyranosyl-(1→6)-O-β-<SMALL>d</SMALL>-fructofuranoside] and was termed stachyobifiose. Among 17 nonprobiotics, 16 nonprobiotics showed lower growth on stachyobifiose than β-GOS. In contrast, among the 16 probiotics, 6 probiotics showed higher growth on stachyobifiose than β-GOS. When compared with raffinose, stachyobifiose was used less by nonprobiotics than raffinose. Moreover, compared with stachyose, stachyobifiose was used less by <I>Escherichia coli</I>, <I>Enterobacter cloacae</I>, and <I>Clostridium butyricum</I>. The average amounts of total short-chain fatty acids (SCFA) produced were in the order of stachyobifiose > stachyose > raffinose > β-GOS. Taken together, stachyobifiose is expected to contribute to beneficial changes of gut microbiota.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2018/jafcau.2018.66.issue-5/acs.jafc.7b04703/production/images/medium/jf-2017-047039_0005.gif'></P>
Geun-Hyoung Choi,Dal-Soon Choi,Su-Myeong Hong,Oh-Kyoung Kwon,Hee Soo Eun,김정한,김진효 한국응용생명화학회 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.3
Contamination levels of 17 polychlorinated dibenzop-dioxins (PCDDs)/polychlorinated dibenzofurans (PCDFs) and 12 dioxin-like polychlorinated biphenyls (DL-PCBs) were investigated on six major fruits consumed in Korea. Dioxin level was monitored on whole fruits. Average concentrations of PCDD/Fs were 0.042 pg/g fresh weight (f.w.) (apple), 0.0094 pg/g f.w. (grape), 0.1843 pg/g f.w. (mandarin orange), 0.2282 pg/g f.w. (peach), 0.1491 pg/g f.w. (pear), and 0.1330 pg/g f.w. (persimmon). For DL-PCBs, the average concentrations were 0.0932 pg/g f.w. (apple), 0.2649 pg/g f.w. (grape), 1.5437 pg/g f.w. (mandarin orange), 0.1942 pg/g f.w. (peach), 0.4591 pg/g f.w. (pear), and 0.4396 pg/g f.w. (persimmon). The major toxic equivalency (TEQ) contributors were identified as 1,2,3,7,8-pentachlorodibenzop-dioxin in PCDD and 2,3,4,7,8-pentachlorodibenzofuran in PCDFs. Most residual DL-PCBs showed no contribution to total TEQ values. The lowest TEQ level was recorded in peach (0.0161pg-TEQWHO/g f.w.), and the highest TEQ level was found in mandarin orange (0.0448 pg-TEQWHO/g f.w.). Total estimated daily intake (EDI) of dioxins from the six fruits was calculated at 3.8159 pg-TEQWHO/day, which is 1.59% of Korean tolerable daily intake based on 60 kg body weight.