도립 진자 시스템의 안정화를 위한 퍼지 제어기의 실현에 관한 연구

최우진,이준탁,소명옥 동아대학교 공과대학부설 생산기술연구소 1996 生産技術硏究所硏究論文集 Vol.1 No.-

In this paper, a hierarchical fuzzy controller for stabilization of the inverted pendulum system is proposed. The facility of this hierarchical fuzzy controller which has a swing-up control mode and a stabilization one, moves a pendulum in an initial natural stable equilibrium point and a cart in an arbitrary position, to an unstable equilibrium point and to a center of rail. Specially, the virtual equilibrium point(Φ_VEq) which describes functionally considers the interactive dynamics between a position of cart and a angle of inverted pendulum is introduced. And comparing with the conventional optimal controller, the proposed hierarchical fuzzy inference structure made substainally the inverted pendulum system robust and stable.

미주신경과 연결된 중추신경로내 Catecholamine성 신경세포에 관한 연구

황광민,이봉희,김은아,김명옥,이봉희,김은아,김명옥,백상호,이부영 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.1

Gentisate 1,2-dioxygenase, in the third naphthalene catabolic gene cluster of Polaromonas naphthalenivorans CJ2, has a role in naphthalene degradation

Lee, Hyo Jung,Kim, Jeong Myeong,Lee, Se Hee,Park, Minjeong,Lee, Kangseok,Madsen, Eugene L.,Jeon, Che Ok Microbiology Society 2011 Microbiology Vol.157 No.10

<P>Polaromonas naphthalenivorans strain CJ2 metabolizes naphthalene via the gentisate pathway and has recently been shown to carry a third copy of gentisate 1,2-dioxygenase (GDO), encoded by nagI3, within a previously uncharacterized naphthalene catabolic gene cluster. The role of this cluster (especially nagI3) in naphthalene metabolism of strain CJ2 was investigated by documenting patterns in regulation, transcription and enzyme activity. Transcriptional analysis of wild-type cells showed the third cluster to be polycistronic and that nagI3 was expressed at a relatively high level. Individual knockout mutants of all three nagI genes were constructed and their influence on both GDO activity and cell growth was evaluated. Of the three knockout strains, CJ2δnagI3 showed severely diminished GDO activity and grew slowest on aromatic substrates. These observations are consistent with the hypothesis that nagI3 may prevent toxic intracellular levels of gentisate from accumulating in CJ2 cells. All three nagI genes from strain CJ2 were cloned into Escherichia coli: the nagI2 and nagI3 genes were successfully overexpressed. The subunit mass of the GDOs were ~36-39 kDa, and their structures were deduced to be dimeric. The K(m) values of NagI2 and NagI3 were 31 and 10 ?M, respectively, indicating that the higher affinity of NagI3 for gentisate may protect the wild-type cells from gentisate toxicity. These results provide clues for explaining why the third gene cluster, particularly the nagI3 gene, is important in strain CJ2. The organization of genes in the third gene cluster matched that of clusters in Polaromonas sp. JS666 and Leptothrix cholodnii SP-6. While horizontal gene transfer (HGT) is one hypothesis for explaining this genetic motif, gene duplication within the ancestral lineage is equally valid. The HGT hypothesis was discounted by noting that the nagI3 allele of strain CJ2 did not share high sequence identity with its homologues in Polaromonas sp. JS666 and L. cholodnii SP-6.</P>

Effects of daily quercetin-rich supplementation on cardiometabolic risks in male smokers

Lee, Kyung-Hea,Park, Eun-Ju,Lee, Hye-Jin,Kim, Myeong-Ok,Cha, Yong-Jun,Kim, Jung-Mi,Lee, Hye-Ran,Shin, Min-Jeong The Korean Nutrition Society 2011 Nutrition Research and Practice Vol. No.

Limited information from human studies indicates that dietary quercetin supplementation influences blood lipid profiles, glycemic response, and inflammatory status, collectively termed cardiometabolic risks. We tested the hypothesis that quercetin-rich supplementation, derived from onion peel extract, improves cardiometabolic risk components in healthy male smokers in a randomized, double blinded, placebo-controlled parallel design. Randomly assigned subjects were instructed to take either the placebo (n=43) or 100 mg quercetin capsules each day (n=49) for 10 weeks. Anthropometric parameters and blood pressure were measured, and blood lipids, glucose, interleukin-6, and soluble vascular cell adhesion molecule-1 (sVCAM-1) were determined at baseline and after 10 weeks of quercetin supplementation. Quercetin-rich supplementation significantly reduced serum concentrations of total cholesterol (P<0.05) and LDL-cholesterol (P<0.01), whereas these effects were not shown in the placebo group. Furthermore, significant increases were observed in serum concentrations of HDL-cholesterol both in the placebo (P<0.005) and quercetin-rich supplementation group (P<0.001); however, changes in HDL-cholesterol were significantly greater in subjects receiving quercetin-rich supplementation than the placebo. Both systolic (P<0.05) and diastolic blood pressure (P<0.01) decreased significantly in the quercetin-rich supplementation group. Glucose concentrations decreased significantly after 10 weeks of quercetin-rich supplementation (P<0.05). In contrast, no effects of quercetin-rich supplementation were observed for the inflammatory markers-IL-6 and sVCAM-1. Daily quercetin-rich supplementation from onion peel extract improved blood lipid profiles, glucose, and blood pressure, suggesting a beneficial role for quercetin as a preventive measure against cardiovascular risk.

Modulation by the GABA _B receptor siRNA of ethanol-mediated PKA-α, CaMKII, and p-CREB intracellular signaling in prenatal rat hippocampal neurons

Lee, Hae Young,Yang, Byoung-Chul,Lee, Eun-Shil,Chung, Jong Ii,Koh, Phil Ok,Park, Moon Seok,Kim, Myeong Ok Korean Association of Anatomists 2011 Anatomy & Cell Biology Vol.44 No.3

<P>Fetal alcohol syndrome (FAS) is a developmental neuropathology resulting from <I>in utero</I> exposure to ethanol; many of ethanol's effects are likely to be mediated by the neurotransmitter γ-aminobutyric acid (GABA). We studied modulation of the neurotransmitter receptor GABA<SUB>B</SUB>R and its capacity for intracellular signal transduction under conditions of ethanol treatment (ET) and RNA interference to investigate a potential role for GABA signaling in FAS. ET increased GABA<SUB>B1</SUB>R protein levels, but decreased protein kinase A-α (PKA-α), calcium/calmodulin-dependent protein kinase II (CaMKII) and phosphorylation of cAMP-response element binding protein (p-CREB), in cultured hippocampal neurons harvested at gestation day 17.5. To elucidate GABA<SUB>B1</SUB>R response to ethanol, we observed the effects of a GABA<SUB>B</SUB>R agonist and antagonist in pharmacotherapy for ethanol abuse. Baclofen increased GABA<SUB>B</SUB>R, CaMKII and p-CREB levels, whereas phaclofen decreased GABA<SUB>B</SUB>R, CaMKII and p-CREB levels except PKA-α. Furthermore, when GABA<SUB>B1</SUB>R was knocked down by siRNA treatment, CaMKII and p-CREB levels were reduced upon ET. We speculate that stimulation of GABA<SUB>B1</SUB>R activity by ET can modulate CaMKII and p-CREB signaling to detrimental effect on fetal brain development.</P>

Varicella-Zoster Virus ORF39 Transmembrane Protein Suppresses Interferon-Beta Promoter Activation by Interacting with STING

Lee Gwang Myeong,Gong Shuang,Seo Seong-Wook,Ko Hyemin,Chung Woo-Chang,Lee Jihyun,Shin Ok Sarah,Ahn Jin-Hyun 한국미생물학회 2023 The journal of microbiology Vol.61 No.2

Varicella-Zoster virus (VZV) causes varicella in primary infection of children and zoster during reactivation in adults. Type I interferon (IFN) signaling suppresses VZV growth, and stimulator of interferon genes (STING) plays an important role in anti-VZV responses by regulating type I IFN signaling. VZV-encoded proteins are shown to inhibit STING-mediated activation of the IFN-β promoter. However, the mechanisms by which VZV regulates STING-mediated signaling pathways are largely unknown. In this study, we demonstrate that the transmembrane protein encoded by VZV open reading frame (ORF) 39 suppresses STING-mediated IFN-β production by interacting with STING. In IFN-β promoter reporter assays, ORF39 protein (ORF39p) inhibited STING-mediated activation of the IFN-β promoter. ORF39p interacted with STING in co-transfection assays, and this interaction was comparable to that of STING dimerization. The cytoplasmic N-terminal 73 amino acids region of ORF39P was not necessary for ORF39 binding and suppression of STING-mediated IFN-β activation. ORF39p also formed a complex containing both STING and TBK1. A recombinant VZV expressing HA-tagged ORF39 was produced using bacmid mutagenesis and showed similar growth to its parent virus. During HA-ORF39 virus infection, the expression level of STING was markedly reduced, and HA-ORF39 interacted with STING. Moreover, HA-ORF39 also colocalized with glycoprotein K (encoded by ORF5) and STING at the Golgi during virus infection. Our results demonstrate that the transmembrane protein ORF39p of VZV plays a role in evading the type I IFN responses by suppressing STINGmediated activation of the IFN-β promoter.

Pulmonary hypertension in patients with Philadelphia-negative myeloproliferative neoplasms: a single-center retrospective analysis of 225 patients

Myeong-Won Lee,류혜원,Yoon-Seok Choi,Ik-Chan Song,Hyo-Jin Lee,Hwan-Jung Yun,Byung Joo Sun,Jin-Ok Jeong,Deog-Yeon Jo 대한혈액학회 2020 Blood Research Vol.55 No.2

BackgroundThe prevalence of pulmonary hypertension (PH) in myeloproliferative neoplasms (MPNs) varies among studies. We analyzed the prevalence of PH in Korean patients with Philadelphia-negative (Ph-) MPNs.MethodsMedical records of patients with Ph- MPNs [essential thrombocythemia (ET), poly-cythemia vera (PV), or primary myelofibrosis (PMF)] visiting a single hospital between 1993 and 2019 were reviewed retrospectively. Transthoracic echocardiographic exami-nation (TTE) results were reviewed and PH was diagnosed according to established guidelines.ResultsOf the 320 MPN (179 ET, 107 PV, and 34 PMF) patients, 225 (121 ET, 83 PV, and 21 PMF) underwent TTE. Of these 225 MPN patients, 19 of 121 (15.7%) ET, 9 of 83 (10.8%) PV, and 6 of 21 (28.6%) PMF patients had PH. PV patients with PH were older [71 (42‒85) vs. 61.5 (26‒91) yr, respectively; P=0.049], predominantly female (male:female ratio, 0.29 vs. 1.96, respectively; P=0.010), had lower hemoglobin levels (15.9±2.6 g/dL vs. 18.4±2.6 g/dL, respectively; P=0.010), and higher platelet counts (616.6±284.2×109/L vs. 437.7±191.7×109/L, respectively; P=0.020) than PV patients without PH. PMF pa-tients with PH had higher monocyte counts (1.3±0.5×109/L vs. 0.8±0.4×109/L, re-spectively; P=0.031) than those without PH. PH was a risk factor for poor survival in PV (HR, 12.4; 95% CI, 1.8‒86.6).ConclusionPH is common in patients with Ph- MPNs and hence, careful screening for PH is warranted.

Relationships between Intermittent Locking History and Self-Reported Bruxism in Temporomandibular Joint

Lee, Myeong-Ok,Lee, Yeon-Hee,Kang, Soo-Kyung,Chun, Yang-Hyun,Hong, Jung-Pyo,Auh, Q-Schick Korean Academy of Orofacial Pain and Oral Medicine 2017 Journal of Oral Medicine and Pain Vol.42 No.2

Purpose: To evaluate aggravating factors of intermittent locking among temporomandibular joint using magnetic resonance imaging (MRI) and diagnostic criteria for temporomandibular disorder (DC/TMD) diagnosis. Methods: A retrospective analysis was conducted of 35 patients with intermittent locking history but normal intra-articular findings between September 2012 and June 2015 in Kyung Hee University Dental Hospital. A standardized DC/TMD assessment was performed on subjects with MRI findings. Clinical findings were assessed on the basis of maximum mouth opening (active & passive), self-reported habits, patients' age, gender, systemic diseases at the initial visit. First, chi square test was used to examine differences with variables and then risk factors for intermittent locking were assessed using multivariate logistic regression. Results: Self-reported bruxism was strongly associated with intermittent locking history. Conclusions: The new DC/TMD protocol is intended for use within any clinical setting and supports the full range of diagnostic activities from screening to definitive evaluation and diagnosis. Self-reported sleep bruxism has been associated with a higher likelihood of intermittent locking. Comorbidity is therefore a factor that must be assessed. It is necessary to consider the amount of contact of the teeth and the duration.

사람 태반에서 GnRH와 GnRH-receptor mRNAs의 발현

김명옥(Myeong Ok Kim),고필옥(Phil Ok Koh),조경제(Gyeong Jae Cho),김해석(Hae Suk Kim),이종학(Jong Hak Lee),김종화(Jong Hwa Kim),백원영(Won Young Paik),최완성(Wan Sung Choi) 대한체질인류학회 1998 해부·생물인류학 (Anat Biol Anthropol) Vol.11 No.1

본 연구는 사람 태반에서 GnRH 와 CnRH -receptor mRNAs 가 어느 세포에서 양현되는지 조사하고, 발현세포의 분포가 임신시기에 따라 어떻게 변화하는지를 조사하기 위하여 수행되었다. 임신중의 각 시기별 태반조직을 대상으로 GnRH 와 CnRH -receptor cDNA 에 대한 cRNA probe 흘 이용하여 tn Stf hybndlzation 을 시행 한 결과에서 다음과 갇은 소견을 얻었다. GnRH mRNA 는 모든 임신시기의 태반 조직내의 cytotrophoblasts, syncytiotrophoblasts, villous stromal cells 에서 동정되었다. GnRH mRNA signals 은 맡기로 갈수록 villous stromas 에서 강한 양성 반응이 관찰되었다. GnRH- receptor mRN A signals는 9-10주의 cytotrophoblasts 와 syncyhotrophoblasts 에서 강한 발현을 나타내었고 말기로 갈수록 감소되었다. GnRH 도 사람의 태반내에서 trophoblasts 에서 극소적 으로 생성되고, CnRH -receptor 는 GnRH 와 유사하게 태반융모의 trophoblast 에서 발현 된다. 또한, CnRB -receptor 가 임신유지에 필요한 융모악 성샘자극호르몬의 분비양상과 유사하게 생성되는 것으로 보아 GnRH 가 융오악 성생자극호르몬의 망성 벚 분비에 있어 조정인자로 작용할 가능성이 있있며, 그 작용에 있어 paracnne 또는 autocnne 역할을 항 것으로 생각된다.

일부 흡연 남성의 식사섭취와 대사증후군 위험도

김명옥(Myeong Ok Kim),이혜진(Hye Jin Lee),박은주(Eun Ju Park),이경혜(Kyung Hea Lee) 대한지역사회영양학회 2010 대한지역사회영양학회지 Vol.15 No.6

This study was intended to investigate the risk of metabolic syndrome (MS) in healthy 92 male smokers (mean age: 44.4 ± 7.8 yrs). We investigated the anthropometric assessment and dietary intake survey for 2 days by 24-recall method, also blood pressure and serum lipids were measured. The average numbers of cigarettes smoking a day were 21.3/d, smoking duration were 21.5 years. The proportion of fat energy was 24.9% and intakes of vitamin B2, folate, calcium, potassum and fiber were lower than KDRI. The body mass index (BMI) and body fat % were 24.8, 23.9% respectively. The systolic (134.1 ± 1.4 mmHg) and the diastolic blood (87.9 ± 1.1 mmHg) pressure were in borderline hypertension. Among biochemical parameters, TG (173.6 ± 9.4 mg/dL) and fasting blood glucose (109.0 ± 2.4 mg/dL) levels were out of normal values. The most occurred problem among the risk factors related to MS was the borderline hypertension (63%) in subjects. Regarding the correlations of anthropometric data with biochemical factors, TG was significantly correlated with the BMI, body fat % and waist circumference. Smoking years showed positive correlation with AI. These results suggest that the smoking habit has significant relations with the risk factors of MS. Therefore, quitting is necessary to prevent MS, and nutrition education and dietary management program are required to prevent the degenerative disease. (Korean J Community Nutr 15(6): 783~795, 2010)