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The role of Sohlh2 during the meiotic process
Miree Park,Min Kim,Sijeong Bae,Eunhye Kim,Miseon Park,Eunjin Lim,Youngsok Choi 한국발생생물학회 2013 한국발생생물학회 학술발표대회 Vol.2013 No.8
The spermatogenesis and oogenesis-specific helix-loop-helix transcription factor 2 (Sohlh2) is exclusively expressed in germ cells of male and female gonad. Sohlh2 acts as a transcriptional factor via its specific DNA binding site, E-box to regulate target genes such as Lhx8, Zp genes, Ngn3. Sohlh2 localize in the female oocyte and in the male spermatogonia. In recent studies, Sohlh2 knockout (KO) mice occurs abnormal spermagoenesis resulting in sperm defect. Sohlh2 KO male mice were infertility due to disruption of numerous gene expression. However, the gene profiles of Sohlh2 KO testes were not characterized and the regulatory mechanism of Sohlh2 was poorly understood. In this study, we analyzed the gene profiles and examined the possible mechanism of Sohlh2 in the spermatogenesis. First, we performed histological analysis such as Hematoxylin and eosin stain, Tunel assay, and Immunohistochemistry to show the onset of disruption of Sohlh2 KO testes. These results showed that Sohlh2 KO testes have atrophic seminiferous tubule due to increased apoptosis at 2 weeks old. And then we analyzed the whole gene profiles in the Sohlh2 KO testes at 2 weeks old. We found that 91 genes were regulated at least 5-fold in knockout testes. Among these, several genes are involved in meiotic process. Quantitative-PCR results are shown that several meiotic factors are significantly down-regulated in 2-weeks-old Sohlh2 KO testes compared with that of wild type mice. Through chromosome spreading assay, we observed that the formation of synaptonemal complex of homologous chromosome during the meiosis in Sohlh2 KO testes was not completed. These suggest that Sohlh2 is critical for regulation of numerous factors including meiotic factors either directly or indirectly. Therefore, mis-regulation of meiotic factors at prophase I of meiosis during spermatogenesis leads to disruption of spermatogenesis in Sohlh2 KO testes. Further studies are needed to look at the mechanism of Sohlh2 for regulation of target genes in detail.
UV,PV 상승을 위한 정보구조분석 가이드라인 개발 및 적용연구 : Joins.com을 중심으로
김미례(Kim mire) 한국HCI학회 2008 한국HCI학회 학술대회 Vol.2008 No.2
PV, UV 하락에 따른 사용성 점검이 대두됨에 따라 전문가를 중심으로 경쟁사이트 비교분석을 통해 정보구조를 유형화하고 시각요소 콘텐트, 사용성 문제 분석을 통해 조인스의 개선방향을 제시한다.
The role of Tdrd12 in the mouse testis
Min Kim,Miree Park,Miseon Park,Ok-Hee Lee,Dong Ryul Lee,Youngsok Choi,Tae Ki Yoon 한국발생생물학회 2013 한국발생생물학회 학술발표대회 Vol.2013 No.8
Tdrd family members contain Tudor domain repeat which is found in polar granules in Drophila. Tdrd12 is one of Tdrd family members. Tdrd12 contains a DEAD-box and a Tudor domain. However, the molecular mechanism and physiological function of Tdrd12 has not been described. To examine the expression pattern of Tdrd12, RT-PCR and Northern blot analysis were performed using total RNAs extracted from tissues; liver, intestine, heart, brain, kidney, lung, brain, uterus, ovary, and testis. The full-length of Tdrd12 was amplified from total RNA from mouse testis and cloning into the cloning vector. Cloned PCR products were purified,sequenced and analyzed using the ABI Prism Sequencer 3130XL. To look into Tdrd12 protein location, rabbit antibodies against mouse Tdrd12 were made using two epitopes: 1st epitope: (318~334)- SQRPNEKPLRLTEKKDC and 2nd epitope: (737~750)- LEAKEDKKARRPLC, and its specificity was tested using tissue extracts including the gonad. Here, we identified that Tdrd12 mRNA is detected in the ovary and testis, but not in other tissues. The size of its transcript is about 4.5kb on the northern blot. Antibody against Tdrd12 detects about 150 kDa protein on the western blot analysis. Immunostaining assay shows that Tdrd12 is localized at the spermatid in the seminiferous tubules. The current study is the first to investigate Tdrd12 expression is limited in the gonad. Thissuggest that Tdrd12 plays a role in the gonad like other known Tdrd family members, Tdrd1, Tdrd6, Tdrd7, and Tdrd9.
변미리(Miree Byun),김묵한(Mookwhan Kim) 서울연구원 2012 서울연구원 정책과제연구보고서 Vol.- No.-
Making Future Cities calls for understanding and evaluating the contemporary urban social system, its recent history, its present dynamics, and its possibilities. Recently, Crisis and uncertainty are increasing surrounding the Korean and global society, and policy forecasting based on future research should be needed and becomes more and more important. The socioeconomic polarization and social exclusion has been the most important social challenges in contemporary cities. Cities are called upon to appropriately respond to this economic, social and political restructuring. Seoul Metropolitan City has faced same challenges. In the past decades, Seoul has experienced dramatic changes derived from unprecedented technological innovation and unfettered economic globalization. These changes undoubtedly have delivered substantial improvements in the lives of peoples in Seoul. Seoul has developed 10 times in economic growth of GRDP during the past 40 years. In the same period, the social and economic polarization also has increased rapidly. Social exclusion, generational unemployment, material poverty, wealth inequalities and ageing population has continued to deteriorate. Fast development brought economic wealth to Seoul, but it gradually disintegrated the kinship neighborhood, shared local identities, and communal traditions as well. What are the policies currently applied and how can there be a more comprehensive, resource-minded urban development? The main aim of this study was to highlight the main challenges for Seoul over the following two decades and to identify the main objectives, instruments and methods Seoul can deploy when tackling them. These questions have been discussed within three methods with the participatory citizen survey(townhall meeting survey), expert survey and empirical data analysis on Seoul’s future. We cover the 20 years of Seoul’s social and economic features, so the temporal scope of this study is by 2030 and its metropolitan area is the spacial extent. In chapter 2, we review the methodology of future study and history of future research. In chapter 3, we analyse future study of cities including Tokyo and United State and recent future trends in Korea. In chapter 4, we propose four mega-trends affecting Seoul, such as population reconfiguration, generation conflict, the mega city region and the climate changes. In chapter 5, we analyse the Mayor’s inaugural address with the text analysis method and issued the enhancing of social quality as the aim of Seoul’s future. In chapter 6, we conduct the citizen participatory survey using the town hall meeting and conduct the expert survey based on the frame of social quality. Finally, we propose the scenarios of social quality in Seoul at the year of 2030. These are the Social Tribe, Me Gray Senior, Nomadingles and Passive Silver.