http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
신평·장림 산단 인근 주민의 PAHs 및 VOCs 노출
윤미라(Mi-Ra Yoon),조혜정(HyeJeong Jo),김근배(GeunBae Kim),장준영(JunYoung Chang),이철우(Chul-Woo Lee),이보은(Bo-Eun Lee) 한국환경보건학회 2021 한국환경보건학회지 Vol.47 No.2
Objectives: This study aims to investigate the atmospheric concentration of polycyclic aromatic hydrocarbons (PAHs) and volatile organic compounds (VOCs) and the urinary concentration of biomarkers in residents near the Shinpyeong·Jangrim Industrial Complex to compare them with those of residents in a control area. Methods: Hazardous air pollutants (PAHs and VOCs) were measured in an exposure area (two sites) and a control area (one site). Urine samples were collected from residents near the industrial complex (184 persons) and residents in the control area (181 persons). Multiple linear regression analysis was used to identify which factors affected the concentration of PAHs and VOCs metabolites. Results: The average atmospheric concentration of PAHs in Shinpyeong-dong and Jangrim-dong was 0.45 and 0.59 ppb for pyrene, 0.15 and 0.16 ppb for benzo[a]pyrene, and 0.29 and 0.35 ppb for dibenz[a,h]anthracene. The average atmospheric concentration of VOCs was 1.10 and 0.99 ppb for benzene, 8.22 and 11.30 ppb for toluene, and 1.91 and 3.05 ppb for ethylbenzene, respectively. The concentrations of PAHs and VOCs in residents near the Shinpyeong·Jangrim Industrial Complex were higher than those of residents in the control area. Geometric means of urinary 2-hydroxyfluorene, 1-hydroxypyrene, methylhippuric acid, and mandelic acid concentrations were 0.45, 0.22, 391.51, and 201.36 μg/g creatinine, respectively. Those levels were all significantly higher than those in the control area (p<0.05). In addition, as a result of multiple regression analysis, even after adjusting for potential confounding factors such as gender and smoking, the concentration of metabolites in urine was high in residents near the Shinpyeong·Jangrim Industrial Complex. Conclusion: The results of this study show the possibility of human exposure to VOCs in residents near the Shinpyeong·Jangrim Industrial Complex. Therefore, continuous monitoring of the local community is required for the management of environmental pollutant emissions.
Juyoung Kim,Youngae Kim,Hyejeong Yun,Hyemin Park,Sun Yeou Kim,Kwang-Gill Le,Sang-Mi Han,Yunhi Cho 한국영양학회 2010 Nutrition Research and Practice Vol.4 No.5
Oral administration of royal jelly (RJ) promotes wound healing in diabetic mice. Concerns have arisen regarding the efficacy of RJ on the wound healing process of normal skin cells. In this study, a wound was created by scratching normal human dermal fibroblasts, one of the major cells involved in the wound healing process. The area was promptly treated with RJ at varying concentrations of 0.1, 1.0, or 5 ㎎/㎖ for up to 48 hrs and migration was analyzed by evaluating closure of the wound margins. Furthermore, altered levels of lipids, which were recently reported to participate in the wound healing process, were analyzed by HPTLC and HPLC. Migration of fibroblasts peaked at 24 hrs after wounding. RJ treatment significantly accelerated the migration of fibroblasts in a dose-dependent manner at 8 hrs. Although RJ also accelerated the migration of fibroblasts at both 20 hrs and 24 hrs after wounding, the efficacy was less potent than at 8 hrs. Among various lipid classes within fibroblasts, the level of cholesterol was significantly decreased at 8 hrs following administration of both 0.1 ㎍/㎖ and 5 ㎎/㎖ RJ. Despite a dose-dependent increase in sphinganines, the levels of sphingosines, ceramides, and glucosylceramides were not altered with any concentration of RJ. We demonstrated that RJ enhances the migration of fibroblasts and alters the levels of various lipids involved in the wound healing process.
Development of Serum-free Medium for Chinese Hamster Ovary Cell Line Producing Recombinant Protein
Na young HONG,Seungjib JEON,Mi Rae KIM,Hyejeong HEO,Sang Yeop LEE,Yong Jin KIM,Tae-Joon JEON,Joon-Chul LEE 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Fetal bovine serum (FBS) has been used as an essential supplement in mammalian cell cultures. However, serumcontaining media have some disadvantages such as unclear composition, poor reproducibility and high risk of microbial contaminations. Recently, serum-free media has gained attention due to its uniform ingredients, excellent stability, and reproducibility. In this study, we developed serum-free media for recombinant Chinese hamster ovary (CHO) cells using design of experiments (DoE). Significant factors were selected using plackett-burman design, and the fractional factorial design was used to examine interactive effects of adopted components. In the optimal condition, the rCHO cells successfully showed higher maximum viable cell density up to 29x105 cells/mL on fourth day. Also, the optimization of components concentration was carried out by the response surface methodology for enhancing growth of rCHO cells.
Improving Culture Performance and Antibody Titer in CHO Cell Fed-batch Culture by Controlling pH
Sang Yeop LEE,Seungjib JEON,Mi Rae KIM,Hyejeong HEO,Byung Cheon LEE,Joon-Chul LEE 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Mammalian cell culture is most common industrial process for the production of recombinant proteins. However, higher cell density and titer are still limited due to factors such as excessive metabolites production, nutrients limitation, and high osmolality. Lactate is the major product from glucose metabolism, and lactate accumulation has been demonstrated to inhibit growth rate and negatively affect antibody production in mammalian cells. To partly overcome this factor, we investigated a simple method using pH control in Chinese hamster ovary (CHO) cells. We performed CHO-DG44 fed-batch cultures under pH conditions (6.9 and 7.0) using rocking bioreactor. As a result, cells grown under low pH condition produced lactate approximately 70% lower than cells grown under high pH condition. We also found that peak viable cell density (PVCD) and final antibody titer were increased in fed-batch culture under low pH condition. Taken together, our results demonstrate that fed-batch culture under pH of 6.9 improves the PVCD and titer in CHO cell culture.Mammalian cell culture is most common industrial process for the production of recombinant proteins. However, higher cell density and titer are still limited due to factors such as excessive metabolites production, nutrients limitation, and high osmolality. Lactate is the major product from glucose metabolism, and lactate accumulation has been demonstrated to inhibit growth rate and negatively affect antibody production in mammalian cells. To partly overcome this factor, we investigated a simple method using pH control in Chinese hamster ovary (CHO) cells. We performed CHO-DG44 fed-batch cultures under pH conditions (6.9 and 7.0) using rocking bioreactor. As a result, cells grown under low pH condition produced lactate approximately 70% lower than cells grown under high pH condition. We also found that peak viable cell density (PVCD) and final antibody titer were increased in fed-batch culture under low pH condition. Taken together, our results demonstrate that fed-batch culture under pH of 6.9 improves the PVCD and titer in CHO cell culture.
Seungjib JEON,Hyun Gi KOH,Mi Rae KIM,Young Jin KIM,Hyejeong HEO,Joon-Chul LEE,Yong Keun CHANG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Microalgae are promising resources for producing value-added products due to their rapid growth rate and high lipid content. Among products obtained from microalgae, the eicosapentaenoic acid (EPA) which is health supplement has gained attention because of the limitations on fish oil production. In this study, we optimized cultivation conditions of Nannochloropsis salina for enhancement of EPA production. The optimization process was carried out by response surface methodology (RSM) for studying the interactive effect of temperature and N concentration (NaNO₃) using central composite design. The RSM results showed an optimal level of 1.28 g/L for NaNO₃ and 21 ℃ for temperature, resulting in maximum EPA productivity of 14.4 ㎎/L/day. To understand the effect of optimal conditions on EPA production, we analyzed the lipid compositions and pigment contents. The glycolipids and chlorophyll a were found to be increased under optimal conditions. We also investigated mRNA expression levels of genes involved in EPA synthesis by quantitative real-time PCR and confirmed their positive contribution to EPA production.
Yong Jin KIM,Seungjib JEON,Na Young HONG,Hyejeong HEO,Sang Yeop LEE,Mi Rae KIM,Joon-Chul LEE 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Transforming Growth Factor-β1 (TGF-β1) is a multifunctional cytokine involved in various biological activities, including differentiation, proliferation, apoptosis and matrix formation in a cell. The demand of TGF-β1 continuously increases in the biomedical field due to various functions of TGF-β1. Vector engineering is a fundamental approach to meet the demand by enhancing the production yield of recombinant proteins. In an effort to produce high amounts of the TGF-β1 for industrial application, we have constructed a mammalian expression system based on a modified pIRES vector with a murine dihydrofolate reductase (mDHFR) gene inserted for MTX-mediated gene amplification system. The signal sequence of TGF-β1 was replaced with that of human azurocidin, and a histidine tag was inserted immediately after the signal sequence to facilitate protein purification. In addition, woodchuck hepatitis virus post-transcriptional regulation element (wPRE) sequence was incorporated at the 3’ end of the TGF-β1 expression cassette for enhanced protein expression. As expected, the expression level of TGF-β1 was increased using engineered vector with wPRE sequences compared to that standard vector. This simple and efficient approach can be used in biomedical industry for the mass production of recombinant protein.