Clinical predictors of chest radiographic abnormalities in young children hospitalized with bronchiolitis: a single center study

Ga Ram Kim,Min Sun Na,Kyung Suk Baek,Seung Jin Lee,Kyung Suk Lee,Young Ho Jung,Hye Mi Jee,Tae Hee Kwon,Man Yong Han,Youn Ho Sheen 대한소아청소년과학회 2016 Clinical and Experimental Pediatrics (CEP) Vol.59 No.12

Purpose: Chest radiography is often performed on patients hospitalized with typical clinical manifestations of bronchiolitis. We aimed to determine the proportion of subjects with pathologic chest radiographic findings and the clinical predictors associated with pathologic chest radiographic findings in young children admitted with the typical presentation of bronchiolitis. Methods: We obtained the following data at admission: sex, age, neonatal history, past history of hospitalization for respiratory illnesses, heart rate, respiratory rate, the presence of fever, total duration of fever, oxygen saturation, laboratory parameters (i.e., complete blood cell count, high-sensitivity C-reactive protein [hs-CRP], etc.), and chest radiography. Results: The study comprised 279 young children. Of these, 26 had a chest radiograph revealing opacity (n=24) or atelectasis (n=2). Multivariate logistic regression analysis showed that after adjustment for confounding factors, the clinical predictors associated with pathologic chest radiographic findings in young children admitted with bronchiolitis were elevated hs-CRP level (>0.3 mg/dL) and past history of hospitalization for respiratory illnesses (all P<0.05). Conclusion: The current study suggests that chest radiographs in young children with typical clinical manifestations of bronchiolitis have limited value. Nonetheless, young children with clinical factors such as high hs-CRP levels at admission or past history of hospitalization for respiratory illnesses may be more likely to have pathologic chest radiographic findings.

표면 근전도 센서 기반 Ptosis 진단 시스템 및 알고리즘 개발

권미성(Mi Sung Kwon),이종하(Jong-Ha Lee),손대구(Dae Gu Son),김아람(A Ram Kim) 대한전자공학회 2015 대한전자공학회 학술대회 Vol.2015 No.6

If the lifting power of eyelid from the levator palpebrae muscle becomes weak, eyelid is drooped and the edge of eyelid cannot be lifted on normal level. This phenomenon is called the ‘Ptosis’. If the person has the ptosis, they use the forehead muscle when the eyes open. In this research, the frontalis EMG was measured for the gaze angle. People with the ptosis was confirmed for the use frontalis muscle more than normal people. Therefore, we propose a system to quantify the presence the ptosis as EMG levels of frontalis muscle.

Novel Antidepressant-Like Activity of Caffeic Acid Phenethyl Ester Is Mediated by Enhanced Glucocorticoid Receptor Function in the Hippocampus

Lee, Mi-Sook,Kim, Young Han,Lee, Bo-ram,Kwon, Seung-Hae,Moon, Won-Jin,Hong, Kwan-Su,Song, Yun Seon,Morita, Kyoji,Hahm, Dae Hyun,Shim, Insop,Her, Song Hindawi Publishing Corporation 2014 Evidence-based Complementary and Alternative Medic Vol.2014 No.-

<P>Caffeic acid phenethyl ester (CAPE) is an active component of propolis that has a variety of potential pharmacological effects. Although we previously demonstrated that propolis has antidepressant-like activity, the effect of CAPE on this activity remains unknown. The present study assessed whether treatment with CAPE (5, 10, and 20 <I>µ</I>mol/kg for 21 days) has an antidepressant-like effect in mice subjected to chronic unpredictable stress via tail suspension (TST) and forced swim (FST) tests. CAPE administration induced behaviors consistent with an antidepressant effect, evidenced by decreased immobility in the TST and FST independent of any effect on serum corticosterone secretion. Western blots, conducted subsequent to behavioral assessment, revealed that CAPE significantly decreased glucocorticoid receptor phosphorylation at S234 (pGR(S234)), resulting in an increased pGR(S220/S234) ratio. We also observed negative correlations between pGR(S220)/(S234) and p38 mitogen-activated protein kinase (p38MAPK) phosphorylation, which was decreased by CAPE treatment. These findings suggest that CAPE treatment exerts an antidepressant-like effect via downregulation of p38MAPK phosphorylation, thereby contributing to enhanced GR function.</P>

황색포도알균의 감염에 따른 세포 내에서의 균의 증식과 Matrix Metalloproteinase (MMP)의 역할

민보람 ( Bo Ram Min ),이영미 ( Young Mi Lee ),박재석 ( Jae Seok Park ),최원일 ( Won Il Choi ),권건영 ( Kun Young Kwon ) 대한결핵 및 호흡기학회 2008 Tuberculosis and Respiratory Diseases Vol.64 No.1

연구배경: 본 연구에서는 황색포도알균이 숙주세포 내로 침입하여 증식하는 정도를 관찰하고자 한다. 이때 세포 외 바탕 단백질의 변화가 수반될 것으로 가설을 설정하고, 이러한 변화에 영향을 미칠 것으로 생각되는 matrix metalloproteinase (MMP)의 발현과 역할에 대해 연구하고자 하였다. 방 법: 황색포도알균은 106∼107 CFU/ml을 105개의 기관지상피세포인 BEAS-2B 세포에 2시간 동안 침입시킨다. 이후 세척으로 세포 밖에 있는 황색포도알균을 제거한 후, BEAS-2B 세포를 다양한 시간 동안(4, 6, 8, 12 시간) 배양한 후 황색포도알균의 집락수(CFU/ml)를 측정하였고, 단백질을 분리하여 세포 외 바탕단백질의 발현 정도와 MMP의 활성도를 측정하였다. 또한 MMP 억제제인 GM6001을 전처치한 후 황색포도알균을 세포에 침입시킨 후 세포 내 에서의 균의 집락수 및 세포 외 바탕단백질의 변화를 관찰 하였다. 결 과: 황색포도알균의 집락을 측정한 결과 4시간과 12시간을 비교해 볼 때 MOI가 증가할수록, 감염시킨 시간이 길수록 숙주세포 내로 침입이 유의하게 증가하였다. BEAS-2B 세포에서 황색포도알균을 침입시킨 시간이 길수록, MOI가 증가할수록 MMP 2 및 MMP 9의 활성도와 dysadherin의 발현은 증가하였고, 이와는 대조적으로 E-cadherin의 발현은 감소하였다. MMP억제제인 GM6001을 전 처치 한 결과 황색포도알균의 세포 내 침입을 유의하게 감소시켰다. 결 론: 황색포도알균이 기관지 상피세포 내로 침입할 때 dysadherin 및 E-cadherin 같은 세포 외 바탕 단백질의 변화를 동반하며, MMP 활성도가 균의 세포 내 침입에 관여하는 것으로 보인다. Background: Staphylococcus aureus frequently colonizes and infects hospitalized patients. Respiratory infections with Staphylococcus aureus are common in patients with compromised airway defenses. However the mechanisms of S. aureus invasion from colonization to the epithelium are unclear. Cell invasion by S. aureus would require destruction of the extracellular matrix, which is believed to be the result of increased matrix metalloproteinases (MMP) activity. Methods: In this study, respiratory epithelial cells were infected with S. aureus. After removing the extracellular bacteria by washing, the internalized bacteria in the cells were assessed by counting the colonized forming units (CFUs). The cell adhesion proteins, dysadherin and E-cadherin, were evaluated by Western blotting. The MMPs in the bacterial invasion were evaluated by pretreating the cells with GM6001, a MMP inhibitor. Results: The internalization of S. aureus was found to be both time and dose dependent, and the increase in MMP 2 and 9 activity was also dependent on the incubation time and the initial amount of bacterial inoculation. The invasion of S. aureus was attenuated by GM6001 after 12 hours incubation with a multiply of infection (MOI)=50. The expression of dysadherin, a membrane protein, was increased in a time and dose dependent manner, while the expression of E-cadherin was decreased. Conclusion: MMPs may mediate the invasion of S. aureus into epithelial cells. (Tuberc Respir Dis 2008;64:22-27)

Nucleation and Growth of the HfO₂ Dielectric Layer for Graphene-Based Devices

Oh, Il-Kwon,Tanskanen, Jukka,Jung, Hanearl,Kim, Kangsik,Lee, Mi Jin,Lee, Zonghoon,Lee, Seoung-Ki,Ahn, Jong-Hyun,Lee, Chang Wan,Kim, Kwanpyo,Kim, Hyungjun,Lee, Han-Bo-Ram American Chemical Society 2015 Chemistry of materials Vol.27 No.17

<P>We investigated nucleation and growth characteristics of atomic layer deposition (ALD) HfO<SUB>2</SUB> on exfoliated and chemical vapor deposition (CVD) graphene by using two Hf precursors, tetrakis(dimethylamino)hafnium (TDMAH) and hafnium tetrachloride (HfCl<SUB>4</SUB>). Experimental results and theoretical calculations indicate that HfO<SUB>2</SUB> nucleation is more favorable on CVD graphene than on exfoliated graphene due to the existence of defect sites. Also, the TDMAH precursor showed much more unfavorable nucleation and growth than HfCl<SUB>4</SUB> due to different initial adsorption mechanisms, affecting lower leakage currents and breakdown electric field. ALD growth characteristics of HfO<SUB>2</SUB> will be fundamentally and practically significant for realizing the fabrication of graphene-based electronic devices.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/cmatex/2015/cmatex.2015.27.issue-17/acs.chemmater.5b01226/production/images/medium/cm-2015-01226v_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/cm5b01226'>ACS Electronic Supporting Info</A></P>

한국 의류제조산업의 효율성에 관한 연구

박우람(Woo Ram Park),김미진(Mi Jin Kim),권오경(Oh Kyoung Kwon),김문영(Mun Young Kim),조우현(Woo Hyun Cho) 한국복식학회 2007 服飾 Vol.57 No.2

Clinical predictors of chest radiographic abnormalities in young children hospitalized with bronchiolitis: a single center study

Kim, Ga Ram,Na, Min Sun,Baek, Kyung Suk,Lee, Seung Jin,Lee, Kyung Suk,Jung, Young Ho,Jee, Hye Mi,Kwon, Tae Hee,Han, Man Yong,Sheen, Youn Ho The Korean Pediatric Society 2016 Clinical and Experimental Pediatrics (CEP) Vol.59 No.12

Purpose: Chest radiography is often performed on patients hospitalized with typical clinical manifestations of bronchiolitis. We aimed to determine the proportion of subjects with pathologic chest radiographic findings and the clinical predictors associated with pathologic chest radiographic findings in young children admitted with the typical presentation of bronchiolitis. Methods: We obtained the following data at admission: sex, age, neonatal history, past history of hospitalization for respiratory illnesses, heart rate, respiratory rate, the presence of fever, total duration of fever, oxygen saturation, laboratory parameters (i.e., complete blood cell count, high-sensitivity C-reactive protein [hs-CRP], etc.), and chest radiography. Results: The study comprised 279 young children. Of these, 26 had a chest radiograph revealing opacity (n=24) or atelectasis (n=2). Multivariate logistic regression analysis showed that after adjustment for confounding factors, the clinical predictors associated with pathologic chest radiographic findings in young children admitted with bronchiolitis were elevated hs-CRP level (>0.3 mg/dL) and past history of hospitalization for respiratory illnesses (all P<0.05). Conclusion: The current study suggests that chest radiographs in young children with typical clinical manifestations of bronchiolitis have limited value. Nonetheless, young children with clinical factors such as high hs-CRP levels at admission or past history of hospitalization for respiratory illnesses may be more likely to have pathologic chest radiographic findings.

MARCKSL1 exhibits anti-angiogenic effects through suppression of VEGFR-2-dependent Akt/PDK-1/mTOR phosphorylation

KIM, BOH-RAM,LEE, SEUNG-HOON,PARK, MI-SUN,SEO, SEUNG-HEE,PARK, YOUNG-MIN,KWON, YOUNG-JOO,RHO, SEUNG-BAE Spandidos Publications 2016 ONCOLOGY REPORTS Vol.35 No.2

<P>Myristoylated alanine-rich C kinase substrate-like 1 (MARCKSL1) plays a pivotal role in the regulation of apoptosis and has been shown to maintain antitumor and metastasis-suppressive properties. In the present study, we examined the effects of MARCKSL1 as a novel anti-angiogenic agent on the inhibition of angiogenesis-mediated cell migration. MARCKSL1 also reduced vascular endothelial growth factor (VEGF)-induced human umbilical vein endothelial cell (HUVEC) proliferation, as well as capillary-like tubular structure formation in vitro. MARCKSL1 disrupted phosphorylation of vascular endothelial growth factor receptor-2 (VEGFR-2) in ovarian tumorigenesis. In addition, MARCKSL1 showed potent anti-angiogenic activity and reduced the levels of VEGF and hypoxia-inducible factor la (HIF-1 alpha) expression, an essential regulator of angiogenesis. Consistently, MARCKSL1 decreased VEGF-induced phosphorylation of the PI3K/Akt signaling pathway components, including phosphoinositide-dependent protein kinase 1 (PDK-1), mammalian target of rapamycin (mTOR), tuberous sclerosis complex 2 (TSC-2), p70 ribosomal protein S6 kinase (p7056K), and glycogen synthase kinase 3 beta (GSK-3 beta) protein. Collectively, our results provide evidence for the physiological/biological function of an endothelial cell system involved in angiogenesis through suppression of Akt/PDK-1/mTOR phosphorylation by interaction with VEGFR-2.</P>

Grape seed proanthocyanidin extract attenuates allergic inflammation in murine models of asthma.

Lee, Taehoon,Kwon, Hyouk-Soo,Bang, Bo-Ram,Lee, Yoon Su,Park, Mi-Young,Moon, Keun-Ai,Kim, Tae-Bum,Lee, Ki-Young,Moon, Hee-Bom,Cho, You Sook Springer 2012 Journal of clinical immunology Vol.32 No.6

<P>Antioxidants have been suggested to alleviate the pathophysiological features of asthma, and grape seed proanthocyanidin extract (GSPE) has been reported to have powerful antioxidant activity.</P>

Glycemic index of dietary formula may not be predictive of postprandial endothelial inflammation

Eun Ju Lee,Ji Yeon Kim,Do Ram Kim,Kyoung Soo Kim,Mi Kyung Kim,Oran Kwon 한국영양학회 2013 Nutrition Research and Practice Vol.7 No.4

The emerging role of endothelial inflammation in diabetes has stimulated research interest in the effects of nutrition on related indices. In the current study we investigated whether the nutrient composition of dietary formula as reflected in glycemic index (GI) may be predictive of postprandial endothelial inflammation in non-diabetic subjects. A double-blinded, randomized, crossover study was conducted in non-diabetic subjects (n = 8/group). Each subject consumed three types of diabetes-specific dietary formulas (high-fiber formula [FF], high-monounsaturated fatty acid (MUFA) formula [MF] and control formula [CF]) standardized to 50 g of available carbohydrates with a 1-week interval between each. The mean glycemic index (GI) was calculated and 3-hour postprandial responses of insulin, soluble intercellular adhesion molecule-1 (sICAM-1), nitrotyrosine (NT) and free fatty acids (FFA) were measured. The MF showed the lowest mean GI and significantly low area under the curve (AUC) for insulin (P = 0.038), but significantly high AUCs for sICAM-1 (P<0.001) and FFA (P < 0.001) as compared to the CF and FF. The FF showed intermediate mean GI, but significantly low AUC for NT (P<0.001) as compared to the CF and MF. The mean GI was not positively correlated to any of the inflammatory markers evaluated, and in fact negatively correlated to changes in FFA (r = -0.473, P = 0.006). While the MF with the lowest GI showed the highest values in most of the inflammatory markers measured, the FF with intermediate GI had a modest beneficial effect on endothelial inflammation. These results suggest that nutrient composition of dietary formula as reflected in the GI may differently influence acute postprandial inflammation in non-diabetic subjects.