생활환경 및 직업환경 노출 유해인자의 면역독성 평가 대체시험법 개발

Acharya Manju(Acharya Manju ),Maharjan Anju(Maharjan Anju ),Lee DaEun(DaEun Lee),K C Pramod(K C Pramod ),Kusma Sarina(Kusma Sarina ),Adhikari Manisha(Adhikari Manisha ),Kim ChangYul(ChangYul Kim),Kim 한국환경보건학회 2022 한국환경보건학회 학술대회 자료집 Vol.2022 No.5

Clinical aspects and management of osteoporosis and fragility fractures in patients with diabetes

Manju Chandran 대한골다공증학회 2017 Osteoporosis and Sarcopenia Vol.3 No.3

Both diabetes and osteoporosis are assuming epidemic proportions throughout the world. Accumulating data suggest that both types 1 and 2 diabetes are associated with an increased risk of fragility fractures. This increased risk appears to be largely independent of bone mineral density (BMD) which is most often noted to be low in type 1 diabetes and normal or increased in type 2 diabetes. This review explores the clinical characteristics of bone fragility in patients with diabetes and highlights studies that have evaluated BMD and fracture prediction tools in these patients. It also briefly reviews the current management principles of osteoporosis in diabetes, with special emphasis on the impact of diabetes medications on bone health as well as explores the efficacy of currently available antiosteoporosis pharmacotherapy in the diabetic population.

Topological studies of molecular interactions of formamide with propanol and butanol at 298.15 K

Manju Rani,Sanjeev Maken 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.5

Molar excess volumes have been measured at 298.15 K for formamide + 1-propanol, 2-propanol, 1-butanol, 2-methyl-1-propanol or 2-methyl-2-propanol mixtures. For an equimolar mixture, molar excess volumes follow the sequence: 1-butanol > 1-propanol > 2-methyl-1-propanol > 2-methyl-2-propanol > 2-propanol. The excess molar volume (VEm ) values have been fitted to Redlich–Kister polynomial equation and other volumetric properties like apparent molar volume, partial molar volume,excess partial molar volume were calculated. The excess volume data have also been rationalized by graph-theoretical arguments. It has been observed that VEm calculated by this approach agree well with the corresponding experimental values. This analysis has further yielded information about the state of aggregation of pure components that is consistent with the existing views on their nature of association. The infrared spectral studies lend further credence to the graph theoretical arguments.

Development of Commercial Trace Moisture Sensor: A Detailed Comparative Study on Microstructural and Impedance Measurements of Two Phases of Alumina

Manju Pandey,Prabhash Mishra,Debdulal Saha,K. Sengupta,S. S. Islam 대한금속·재료학회 2014 ELECTRONIC MATERIALS LETTERS Vol.10 No.2

Porous alumina thin film has been investigated in order to find its sensitivity in the trace moisture level. Alumina, with two different phases were obtained using sol-gel process and fired between 400°C to 1000°C. FESEM, BET, AFM and XRD techniques were employed for microstructural characterization of the sintered thin film in two phases. A detailed analysis is done to assess the superiority of gamma (γ) - over alpha (α) phase using impedance spectroscopy. It was observed that gamma phase is more sensitive towards trace level moisture sensing.

Synthesis and characterization of low-cost ceramic membranes from fly ash and kaolin for humic acid separation

Manju Rawat,Vijaya Kumar Bulasara 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.3

Ceramic microfiltration membranes were prepared using five different compositions formulated with different amounts of fly ash and kaolin and sintered at 900 oC. The SEM analysis evidenced a large number of small pores on the surface of kaolin-rich membranes. The M4 membrane prepared using 25% fly ash and 50% kaolin was found to be optimum as it had a good combination of pore size (0.885 μm), porosity (42.7%), mechanical strength (43.6MPa), and chemical stability (<3% weight loss in acid and 0.02% in base), and this membrane was successfully applied in separation of humic acid from water. The permeate flux data fitted very closely with cake-filtration model, indicating the formation of a cake layer on membrane surface. Membrane fouling was found to be reversible and easily negated by cleaning and backflushing. The regenerated membrane showed better rejection of humic acid than fresh membrane with a flux recovery of above 80%.

Measurement and modeling of viscosity for binary mixtures of diisopropyl ether with n-alkanes (C7-C10)

Manju Rani,Sanjeev Maken,박소진 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.9

The kinematic viscosity (ν) of binary mixture of diisopropyl ether+n-heptane or n-octane or n-nonane or n-decane was measured at 298.15 K, 308.15 and 318.15 K. Using experimental data, the deviation in kinematic viscosity (Δν) was calculated. The Δν follows the sequence: n-heptane<n-octane<n-nonane<n-decane. The kinematic viscosity data were correlated by using various empirical equations suggested by Heric-Brewer, Krishnan-Ladda, Lulian et al. Predicted data using these empirical equations agree well with the experimental data. Kinematic viscosity data was also analyzed by applying the McAllister equation. Dynamic viscosity (η) for binary mixture of diisopropyl ether (DIPE)+n-heptane or n-octane was calculated using density data reported in earlier papers. Deviation in dynamic viscosity (Δη) was also calculated. Bloomfield and Dewan model was used to predict dynamic viscosity, and the predicted values agree well with experimental data for the present binary systems. The Δν and Δη values were fitted to Redlich-Kister polynomial equation. The effect of temperature on kinematic viscosity was also studied.

Comparison of the Osteoporosis Self-Assessment Tool for Asians and the fracture risk assessment tool - FRAX to identify densitometric defined osteoporosis: A discriminatory value analysis in a multi-ethnic female population in Southeast Asia

Manju Chandran,Yun Ann Chin,Kuan Swen Choo,Wan Chen Ang,Xiao Feng Huang,Xiao Ming Liu,Donovan Tay,Tin Kyaw Kyaw Aung,Amin Ali,Win Pa Pa Thu,Susan Logan,Sean Xuexian Yan,Sarath Lekamwasam,Ying Hao 대한골다공증학회 2020 Osteoporosis and Sarcopenia Vol.6 No.2

Objectives: The accuracy of FRAX® as a screening tool to identify osteoporosis and how it compares with tools such as Osteoporosis Self-Assessment Tool for Asians (OSTA), in Southeast Asian women has so far been unexplored. We aimed to determine the FRAX® thresholds that accurately identify densitometric osteoporosis and to compare its performance with that of OSTA for this purpose. Methods: Singaporean postmenopausal women (n ¼ 1056) were evaluated. FRAX® Major Osteoporotic Fracture Probability (MOFP), Hip Fracture Probability (HFP) scores, and OSTA indices were calculated. Receiver operating characteristic (ROC) curves were constructed and via the Youden index, the optimal cut-off points of balanced sensitivity and specificity for dual energy X-ray absorptiometry (DXA)-defined osteoporosis were identified and the performance characteristics were compared. Results: A FRAX® MOFP threshold of ≥3.7% had sensitivity, specificity, positive predictive value and negative predictive value of 0.78 (0.73-0.83), 0.63 (0.59-0.66), 0.4 (0.36-0.44), and 0.9 (0.87-0.92), respectively in identifying osteoporosis. The corresponding values for a HFP threshold of 0.6% were 0.85 (0.80-0.89), 0.58 (0.55-0.62), 0.39 (0.35-0.43), and 0.92 (0.9-0.94) and that for an OSTA index cut-off of ≥ -1.2 were 0.76 (0.70-0.81), 0.74 (0.71-0.77), 0.48 (0.43-0.54), and 0.91 (0.88-0.93). The area under the ROC curves were 82.8% (79.9%-85.6%), 77.6% (74.2%-81%), and 79.6% (76.5%-82.8%) for OSTA, MOFP, and HFP thresholds respectively. Conclusions: FRAX® and OSTA perform comparably in identifying osteoporosis in our population. OSTA has only 2 parameters and may be simpler to use. However, FRAX® may also have a role in primary screening to identify the postmenopausal woman to be referred for DXA scanning and may help facilitate fracture risk reduction discussions with the patient.

화학물질들의 면역독성 모니터링을 위한 THP-1 수지상세포주 활용

( Manju Acharya ),( Ravi Gautam ),( Anju Maharjan ),조지훈 ( Ji-hun Jo ),이다은 ( Daeun Lee ),박현지 ( Hyunji Park ),김유빈 ( Yubin Kim ),박성민 ( Sungmin Park ),허용 ( Yong Heo ) 대구가톨릭대학교 자연과학연구소 2020 자연과학연구논문집 Vol.18 No.1

Alternative test methods have been developed for various areas of toxicologic evaluation including skin sensitization or irritation, eye irritation, genotoxicity, or carcinogenicity. Whereas, alternative test method, especially based on in vitro approach, for screening immunotoxic substances was not actively challenged and therein, no test guideline has not been officialized internationally. THP-1 dendritic cell line was chosen for developing in vitro alternative test method for screening immunotoxic chemical or medicinal substances. Regarding major pathway for immune suppression or non-specific immune enhancement, antigen presenting cell is considered as most important target immune cells. Two test substances representing immune-enhancement(ribavirin) or immune-suppression (cyclophosphamide) were tested whether production of cytokines or chemokines is differentially modulated through supplementation of 0.01x, 0.1x, and 0.5x 75% cell viability(CV75) concentration of those compounds. Cell culture supernatants were collected at 24 h after the activation with 1 ㎍/㎖ lipopolysaccharide. Relative cytokine production levels (% versus each vehicle control, RCPL) were calculated. Production of all 27 cytokines evaluated was confirmed, which included IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7,IL-8, IL-9,IL-10, IL-12, IL-13, IL-15, IL-17, Eatoxin, FGF basic, G-CSF, GM-CSF, IFNγ, IP-10, MCP-1, PDGF-BB, MIP-1α, MIP-1β, RANTES, TNFα, VEGF. Treatment of ribavirin demonstrated all the RCPL values over 100% at 0.01x and 0.1x CV75 concentration. Treatment of cyclosphosphamide demonstrated the RCPL values below 100%except IL-12(0.01x), IL-13(0.1x), MCP-1(0.01x), TNFα (0.01x, 0.1x, and 0.5x), and VEGF(0.01x, 0.1x, and 0.5x). This findings suggest that THP-1 cell line is a valuable cell line to develop an in vitro alternative test method for screening immunotoxic chemical substances.

Application of polyaniline as enzyme based biosensor

Manju Gerard,말호트라 한국물리학회 2005 Current Applied Physics Vol.5 No.2

The PANI lms have been synthesized electrochemically and are used as matrix for immobilization of glucose oxidase (GOD)and lactate dehydrogenase (LDH) enzymes. The temporal aspects of anion self-exchange in PANI lms have been investigated. Theexchange of bulkier tosylateferricyanide ion with Cl. ion has been monitored by photometry and electrochemical techniques. Therelative changes in porosity brought about by self-exchange have been experimentally determined to be 323 and 2125/k in tosylate-exchanged and ferricyanide-exchanged polyaniline lms, respectively. It is seen that the polyaniline lms exhibit enhanced loading ofglucose oxidase after a self-ion exchange, and, hence they can be used for the fabrication of a third generation glucose biosensor.Lactate is determined by the photometric detection of NADH formed in the reaction catalysed by LDH. Studies have been car-ried out with PANI as a matrix for the immobilization of LDH and its feasibility as a biosensor. The results of the photometric andamperometric measurements conducted on such LDH/PANI electrodes show a response to pyruvate concentration upto 0.45 mM, aresponse time of 90 s and a shelf life of about two weeks..

Protective effect of silymarin in streptozotocin-induced diabetic dyslipidaemia in rats

Manju Sharma,K. K. Pillai,Tarique Anwer,Abul Kalam Najmi,Syed Ehtaishamul Haque,Yasmin Sultana 경희대학교 융합한의과학연구소 2010 Oriental Pharmacy and Experimental Medicine Vol.10 No.3

The present study investigated the effect of silymarin, a flavonoid, on streptozotocin (STZ) - induced diabetic dyslipidaemia in rats. Experimental diabetes was induced by a single intraperitoneal injection of STZ (60 mg/kg). Silymarin (25 mg/kg and 50 mg/kg) was orally administered to diabetic rats for a period of 15 days. Blood glucose levels, serum lipid profile and liver glycogen levels were estimated following the established procedures. Biochemical observations were supplemented with histological examination of liver sections. Oral administration of silymarin to diabetic rats significantly (P < 0.001) decreased the blood glucose levels (259.99 ± 23.64 vs. 99.90 ±2.62 [25 mg] & 89.17 ± 3.32 [50 mg]). The most interesting finding was the significant (p < 0.001)increase in HDL-cholesterol levels (26.99 ± 0.61 vs. 40.55 ± 0.52 [25 mg] & 41.12 ± 0.37 [50 mg])whereas, there was a significant decrease in serum total cholesterol (TCh), triglycerides (TG), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) cholesterol levels observed in silymarin treated diabetic rats. STZ treatment caused significant degeneration of liver parenchyma,which was normalized to near normal morphology by administration of silymarin. The findings indicate that silymarin effectively improved the overall lipid profile and restored the glycogen stores in the liver of STZ-induced diabetic rats, in a dose dependent manner. The results indicate existence of abnormalities in lipid metabolism in STZ-induced diabetic rats and suggest a protective effect of silymarin in this animal model.