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Ma Xingyuan,Zheng Wenyun,Wang Tianwen,Wei Dongzhi,Ma Yushu The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.3
The Escherichia coli heat-labile enterotoxin B subunit (HLT-B) is one of the most powerful mucosal immunogens and known mucosal adjuvants. However, the induction of high levels of HLT-B expression in E. coli has proven a difficult proposition. Therefore, in this study, the HLT-B gene was cloned from pathogenic E. coli and expressed as a fusion protein with GST (glutathion S-transferase) in E. coli BL2l (DE3), in an attempt to harvest a large quantity of soluble HLT-B. The culture conditions, including the culture media used, temperature, pH and the presence of lactose as an inducer, were all optimized in order to obtain an increase in the expression of soluble GST-rHLT-B. The biological activity of the purified rHLT-B was assayed in a series of GMI-ELISA experiments. The findings of these trials indicated that the yield of soluble recombinant GST-rHLT-B could be increased by up to 3-fold, as compared with that seen prior to the optimization, and that lactose was a more efficient alternative inducer than IPTG. The production of rHLT-B, at 92 % purity, reached an optimal level of 96 mg/l in a 3.7 L fermentor. The specific GM1 binding ability of the purified rHLT-B was determined to be almost identical to that of standard CTB.
cDNA Cloning and Expression Analysis of a Novel Human F-Box Only Protein
Haipeng Cheng,Yushu Ma,Xiaohua Ni,Min Jiang,Lingchen Guo,Wei Jin,Weiwen Xu,Gentao Cao,Chaoneng Ji,Kang Ying,Shaohua Gu,Yuhong Ma,Yi Xie,Yumun Mao 한국분자세포생물학회 2002 Molecules and cells Vol.14 No.1
F-box proteins are an expanding family of eukaryotic proteins that are characterized by an approximately 40 amino acid motif. Some F-box proteins are critical for the controlled degradation of cellular regulatory proteins. During a large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a cDNA clone that encodes a novel F-box protein. It showed a 90.0% identity with the previously isolated mouse F-box protein16 at the amino acid level. Northern blot analysis showed no detectable expression, while re-verse transcription-polymerase chain reaction analysis indicated that FBXO16 was expressed in the heart, spleen, and colon. By mapping, we localized the FBXO16 gene to the human chromosome 8p12. The FBXO16 gene consisted of 9 exons that spanned 67,816 bp of human genomic DNA.
Chao Chen,Yumei Wang,Chun Su,Xinqing Zhao,Ming Li,Xiaowei Meng,김영우,양승환,Yushu Ma,Dong-Zhi Wei,서주원 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.1
Passalora fulva (or Fulvia fulva) is the causalmicroorganism of tomato leaf mold, the outbreak of whichoccurs worldwide in greenhouse especially when humidityis high. However, studies on antifungal agents of P. fulvaare still very limited. In this study, a marine-derivedStreptomyces albidoflavus strain L131 showing potentinhibitory activities against P. fulva was identified andcharacterized. The active antifungal components wereobtained, and studies on the antifungal mechanisms of thecrude extract showed that the antifungal metabolites ofL131 caused damage of hyphae and spore development, aswell as plasma membrane of P. fulva. In addition, accumulationof endogenous reactive oxygen species of the leafpathogen was also observed after treatment by cultureextracts of L131. To our knowledge, this is the first reporton the studies of the antifungal mechanisms againstP. fulva, which benefit further development of biocontrolagent against tomato leaf mold disease.
Chen, Chao,Wang, Yumei,Su, Chun,Zhao, Xinqing,Li, Ming,Meng, Xiaowei,Jin, Yingyu,Yang, Seung-Hwan,Ma, Yushu,Wei, Wei,Joo-Won, Suh 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.1
Passalora fulva (or Fulvia fulva) is the causal microorganism of tomato leaf mold, the outbreak of which occurs worldwide in greenhouse especially when humidity is high. However, studies on antifungal agents of P. fulva are still very limited. In this study, a marine-derived Streptomyces albidoflavus strain L131 showing potent inhibitory activities against P. fulva was identified and characterized. The active antifungal components were obtained, and studies on the antifungal mechanisms of the crude extract showed that the antifungal metabolites of L131 caused damage of hyphae and spore development, as well as plasma membrane of P. fulva. In addition, accumulation of endogenous reactive oxygen species of the leaf pathogen was also observed after treatment by culture extracts of L131. To our knowledge, this is the first report on the studies of the antifungal mechanisms against P. fulva, which benefit further development of biocontrol agent against tomato leaf mold disease.