Fabrication of composite nanofiltration membranes with enhanced structural stability for concentrating oligomeric proanthocyanidins in ethanol aqueous solution

Yanyan Ma,Yanlei Su,Yafei Li,Zhongyi Jiang 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.9

Composite nanofiltration (NF) membranes with enhanced structural stability were fabricated and used to concentrate oligomeric proanthocyanidins (OPC) in ethanol solution. The composite NF membranes were prepared by interfacial polymerization of piperazine (PIP) with trimesoyl chloride (TMC), upon the porous supports of polyethersulfone (PES) and polyvinyl formal (PVF) blend membranes. The active layers of composite NF membranes were covalently linked to porous supports owing to hydroxyl groups of PVF upon support surface, which could participate in the interfacial polymerization reaction. The pure water fluxes of the composite NF membranes reached 34.9 L/m2h, while the rejections of Na2SO4 and orange GII were 92.7% and 98.4%, respectively. The enhanced structural stability of the composite NF membranes were confirmed by long-term immersion experiment in ethanol. NF concentration process was considered as a potential alternative to conventional evaporation concentration process for OPC concentration in ethanol solution.

1-methylcyclopropene treatment improves postharvest quality and antioxidant activity of Prunus domestica L. cv. Ximei fruit

Ma Yanyan,Zhang Weida,CHENGSHAOBO,Yang Wanting,Liu Yuxing,Yang Shengyu,Zhang Xinling,Guo Minrui,Chen Guogang 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.6

Prunus domestica L. cv. Ximei fruit perishes quickly due to intense metabolic activity after being harvested. To prolong shelf life and maintain fruit quality, the eff ects of 1-methylcyclopropene (1-MCP) treatment on P. domestica fruit during storage at 4 ± 1 °C were investigated. The results showed that the soluble solid content (SSC), respiratory rate (29.8%), ethylene production (27.2%), anthocyanin content, malonaldehyde content (MDA), hydrogen peroxide content (H 2O2), and superoxide anion activity (O 2·−) of P. domestica fruit were all signifi cantly reduced by 1-MCP treatment (1.0 μL L −1), while the content of ascorbic acid and total phenol, and the activity of SUPEROXIDE DISMUTASE (SOD, 61.3%), CATALASE (CAT, 39.0%), ASCORBATE PEROXIDASE (APX, 23.7%), and PEROXIDASE (POD, 38.0%) increased compared to untreated fruit after 35 days of cold storage. Overall, 1-MCP treatment could maintain high postharvest quality and anti- oxidant activity in P. domestica fruit

Preparation and Antioxidant Activities In Vitro of a Designed Antioxidant Peptide from Pinctada fucata by Recombinant Escherichia coli

( Yanyan Wu ),( Yongkai Ma ),( Laihao Li ),( Xianqing Yang ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.1

An antioxidant peptide derived from Pinctada fucata meat using an Alcalase2.4L enzymatic hydrolysis method (named AOP) and identified by LC-TOF-MS has promising clinical potential for generating cosmetic products that protect skin from sunshine. To date, there have been few published studies investigating the structure-activity relationship in these peptides. To prepare antioxidant peptides better and improve their stability, the design and expression of an antioxidant peptide from Pinctada fucata (named DSAOP) was studied. The peptide contains a common precursor of an expression vector containing an α-helix tandemly linked according to the BamHI restriction sites. The DNA fragments encoding DSAOP were synthesized and subcloned into the expression vector pET-30a (+), and the peptide was expressed mostly as soluble protein in recombinant Escherichia coli. Meanwhile, the DPPH radical scavenging activity, superoxide radical scavenging activity, and hydroxyl radical scavenging activity of DSAOP IC50 values were 0.136 ± 0.006, 0.625 ± 0.025, and 0.306 ± 0.015 mg/ml, respectively, with 2-fold higher DPPH radical scavenging activity compared with chemosynthesized AOP (p < 0.05), as well as higher superoxide radical scavenging activity compared with natural AOP (p < 0.05). This preparation method was at the international advanced level. Furthermore, pilot-scale production results showed that DSAOP was expressed successfully in fermenter cultures, which indicated that the design strategy and expression methods would be useful for obtaining substantial amounts of stable peptides at low costs. These results showed that DSAOP produced with recombinant Escherichia coli could be useful in cosmetic skin care products, health foods, and pharmaceuticals.

In Vivo/In Vitro Properties of Novel Antioxidant Peptide from Pinctada fucata

( Yongkai Ma ),( Kehui Huang ),( Yanyan Wu ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.1

Due to the potential of antioxidants to scavenge free radicals in human body, it is important to be able to prepare antioxidant peptides that meet the industrial requirements for cosmetics and food. Here, we determined in vivo/in vitro activities of antioxidant peptide from P. fucata (PFAOP) prepared by bio-fermentation method. The antioxidant property test results showed the DPPH, hydroxyl, superoxide radical-scavenging, and cellular antioxidant activity. EC50 values of PFAOPs were 0.018 ± 0.005, 0.126 ± 0.008, 0.168 ± 0.005, and 0.105 ± 0.005 mg/ml, respectively, exhibiting higher antioxidant activities than glutathione (p < 0.05). Moreover, anti-proliferation and cytotoxicity activity results illustrated PFAOP has a potent anti-proliferative activity against HepG2, Caco-2, and MCF-7 carcinoma cells with no cytotoxicity. Moreover, the protocols we developed in this work demonstrated several excellent advantages in PFAOP preparation compared to enzymatic hydrolysis or chemical synthesis methods and provide a theoretical foundation for higher-value application of marine-derived functional peptides.

Nitric oxide synthase inhibitors: a review of patents from 2011 to the present

Yang, Yanyan,Yu, Tao,Lian, Yu-ji,Ma, Rujun,Yang, Sungjae,Cho, Jae Youl Informa UK, Ltd. 2015 Expert opinion on therapeutic patents Vol.25 No.1

<P><B><I>Introduction:</I></B> Nitric oxide synthases (NOSs) are a family of enzymes that play an essential role in synthesizing nitric oxide (NO) by oxidizing <SMALL>L</SMALL>-arginine. As previously reported, NO is a significant mediator in cellular signaling pathways. It serves as a crucial regulator in insulin secretion, vascular tone, peristalsis, angiogenesis, neural development and inflammation. Due to its important role, the inhibition of these vital enzymes provides, as tools, the opportunity to gain an insight into potential therapeutic applications targeting NOSs.</P><P><B><I>Areas covered:</I></B> This paper reviews the patent literature between 2011 and mid-2014 that specified inhibitors of NOS family members as the significant targets. Google and Baidu search engines were used to find relevant patents and clinical information using NOSs or NOS inhibitor as search terms.</P><P><B><I>Expert opinion:</I></B> Considerable recent progress has been made in the development of NOS inhibitors with pharmacodynamic and pharmacokinetic properties, and such development is likely to continue. The patented compounds attenuated mostly embodying evidence from <I>in vitro</I> and <I>in vivo</I> trials that demonstrate good potential for future clinical human trials and industrial applications. Furthermore, new techniques such as X-ray ligand crystallographic study and structure-activity relationship were popularly utilized, which give new insights for developing novel, safe, efficient and selective NOS inhibitors.</P>

3D Bioprinted GelMA/PEGDA Hybrid Scaffold for Establishing an In Vitro Model of Melanoma

( Jiahui Duan ),( Yanyan Cao ),( Zhizhong Shen ),( Yongqiang Cheng ),( Zhuwei Ma ),( Lijing Wang ),( Yating Zhang ),( Yuchuan An ),( Shengbo Sang ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.4

Due to the high incidence of malignant melanoma, the establishment of in vitro models that recapitulate the tumor microenvironment is of great biological and clinical importance for tumor treatment and drug research. In this study, 3D printing technology was used to prepare GelMA/PEGDA composite scaffolds that mimic the microenvironment of human malignant melanoma cell (A375) growth and construct in vitro melanoma micro-models. The GelMA/PEGDA hybrid scaffold was tested by the mechanical property, cell live/dead assay, cell proliferation assay, cytoskeleton staining and drug loading assay. The growth of tumor cells in two- and three-dimensional culture systems and the anti-cancer effect of luteolin were evaluated using the live/dead staining method and the Cell Counting Kit-8 (CCK-8) method. The results showed a high aggregation of tumor cells on the 3D scaffold, which was suitable for long-term culture. Cytoskeleton staining and immunofluorescent protein staining were used to evaluate the degree of differentiation of tumor cells under 2D and 3D culture systems. The results indicated that 3D bioprinted scaffolds were more suitable for tumor cell expansion and differentiation, and the tumor cells were more aggressive. In addition, luteolin was time- and dose-dependent on tumor cells, and tumor cells in the 3D culture system were more resistant to the drug.

The Emergence of the 16S rRNA Methyltransferase RmtB in a Multidrug-Resistant Serratia marcescens Isolate in China

Xue-Jiao Ma,Jia-Bin Li,Jun Cheng,Haifei Yang,Yanyan Liu,Qing Mei,Ying Ye,Hong-Ru Li 대한진단검사의학회 2015 Annals of Laboratory Medicine Vol.35 No.1

A review of the preparation and applications of MnO2 composites in formaldehyde oxidation

Shengnan Guan,Wenzhi Li,Jianru Ma,Yanyan Lei,Yuanshuai Zhu,Qifu Huang,Xiaomeng Dou 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.66 No.-

This paper reviews several important preparation methods, and several kinds of MnO2 composites, which is efficient in formaldehyde catalytic oxidation. The preparation of MnO2 composites discussed in this article refer to sol–gel method, rheological phase reaction method, micro-emulsion method, chemical coprecipitation method, solid-phase synthesis and template method. After a presentation of methods for the preparation of MnO2, a review of the performance on formaldehyde catalytic oxidation over (I) manganese oxides; (II) metal–MnO2 oxide composites; (III) graphene–MnO2 composites is provided. The mechanisms for catalytic oxidation of HCHO over MnO2 composites, future directions and potential hotspots are also discussed to facilitate understanding.

Enhanced corrosion resistance by polypyrrole and Ti3C2Tx-acrylic epoxy double-layer coating for 304SS bipolar plates of PEMFC

Haifeng Bian,Guoli Zhang,Qingxi Zhai,Yanyan Du,Yujie Ma,Beibei Yang,Shaochun Tang,Duan Bin,Xiangkang Meng,Hongbin Lu 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.122 No.-

Stainless steel (SS) is a promising material for designing bipolar plates (BPs), but their further applicationis limited by serious corrosion problems in the acidic environment. Ti3C2Tx is expected to be used for SSBPs coatings, while galvanic corrosion will occur after the damage of Ti3C2Tx coating. Herein, a PPY/Ti3C2Tx-AE double-layer coating (DC) is well-designed and prepared on 304SS BP, which is composedof an inner electropolymerized PPY layer and an outer Ti3C2Tx-acrylic epoxy layer. When tested in0.2 M HCl solution, the corrosion potential and corrosion current density of the DC are 38 mV and0.00927 lA cm2 respectively, which are superior to those of the PPY coating and the Ti3C2Tx coating. Moreover, the DC presents the best long-term stability among the three coatings. The excellent corrosionresistance is attributed to the barrier and anodic protection effects as well as the solution of galvanic corrosion. The new coating system provides a new insight into the design of DC coatings of SS BPs.

Characterization of clutch traits and egg production in six chicken breeds

Shi Lei,Li Yunlei,Isa Adam Mani,Ma Hui,Yuan Jingwei,Wang Panlin,Ge Pingzhuang,Gong Yanzhang,Chen Jilan,Sun Yanyan 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.6

Objective: The better understanding of laying pattern of birds is crucial for developing breed-specific proper breeding scheme and management. Methods: Daily egg production until 50 wk of age of six chicken breeds including one layer (White Leghorn, WL), three dual-purpose (Rhode Island Red, RIR; Columbian Plymouth Rock, CR; and Barred Plymouth Rock, BR), one synthetic dwarf (DY), and one indigenous (Beijing-You Chicken, BYC) were used to characterize their clutch traits and egg production. The age at first egg, egg number, average and maximum clutch length, pause length, and number of clutches and pauses were calculated accordingly. Results: The egg number and average clutch length in WL, RIR, CR, and BR were higher than those in DY and BYC (p<0.01). The numbers of clutches and pauses, and pause length in WL, RIR, CR, and BR were lower than those in DY and BYC (p<0.01). The coefficient variations of clutch length in WL, RIR, CR, and BR (57.66%, 66.49%, 64.22%, and 55.35%, respectively) were higher than DY (41.84%) and BYC (36.29%), while the coefficient variations of egg number in WL, RIR, CR, and BR (9.10%, 9.97%, 10.82%, and 9.92%) were lower than DY (15.84%) and BYC (16.85%). The clutch length was positively correlated with egg number (r = 0.51 to 0.66; p<0.01), but not correlated with age at first egg in all breeds. Conclusion: The six breeds showed significant different clutch and egg production traits. Due to the selection history, the high and median productive layer breeds had higher clutch length than those of the less productive indigenous BYC. The clutch length is a proper selection criterion for further progress in egg production. The age at first egg, which is independent of clutch traits, is especially encouraged to be improved by selection in the BYC breed.