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Liu, Mingfei,Wang, Junli,Tian, Birui,Huang, Jingjing,Zhang, Rongrong,Lin, Yuxing,Xiao, Zefeng Korean Society of Horticultural Science 2017 원예과학기술지 Vol.35 No.2
Tea is one of the most consumed beverages worldwide and the relatively high levels polyphenols is benefit for health. In this study, we developed an efficient system for proliferation of callus from 'Anji Baicha', a cultivar of tea (Camellia sinensis). Callus tissue was initially induced by culturing leaf explants on medium containing different plant growth regulators. For callus induction, thidiazuron (TDZ) was more effective than 2,4-dichlorophenoxyacetic acid (2,4-D), ${\alpha}-naphthalene$ acetic acid (NAA), and $N^6-benzyladenine$ (BA). The frequency of callus induction from leaf explants reached 90.21% on $1.0mg{\cdot}L^{-1}$ TDZ and the developed callus was reddish and friable. We also tested the effect of different concentrations of NAA, 2,4-D, indole 3-acetic acid (IAA), BA, and TDZ, alone and in combinations, on callus proliferation. Medium supplemented with TDZ in combination with IAA was suitable for callus proliferation and accumulation of tea polyphenols. The growth index value and tea polyphenol content of callus cultured on MS medium containing $0.5mg{\cdot}L^{-1}$ TDZ and $1.0mg{\cdot}L^{-1}$ IAA was maximally 1,351% and 23.24%, respectively, and the relative abundance of epicatechin was as high as 17.449%. We also measured the antioxidant activity of all samples and the callus with the highest tea polyphenol content also exhibited high potential radical scavenging activity.
Liu Jianbai,Wu Han,Yi Jiequn,Li Jihu,Cheng Yinjie,Cheng Yinjie,Sun Donglei,An Yuxing 한국곤충학회 2022 Entomological Research Vol.52 No.2
Chilo sacchariphagus is one of the most harmful pests of sugarcane, sorghum, corn, and other crops, in China and other countries and regions. In order to elucidate the molecular mechanisms involved in the sexually dimorphic development of C. sacchariphagus, transcriptome data of female and male adults were obtained. A total of 127,812,174 and 126,593,664 clean reads of males and females were arranged into 45,693 and 37,034 unigenes for males and females, respectively, 26,949 of which were annotated. Candidate genes involved in sexual development were identified and analysed. Statistical analysis revealed that 18,642 genes were differentially expressed in females and males, 9,307 of which were up-regulated in males and 9,335 of which were up-regulated in females. As indicated by GO classification, DEGs were mainly involved in cell part, cellular process and binding. KEGG enrichment analysis showed that 6,037 DEGs were assigned to 295 metabolic pathways. Based on annotation and transcriptome data, we identified twenty-two sex determining genes, of which Csactra2, Csacemc, Csacfru, Csacix, Csacovo, CsacdsxM,andCsacsxl showed a higher expression in males than that in females, while CsacgroX4, Csacfem, CsacgroX9, Csacmsl1, Csacmsl2, Csacmsl3, Csacotu, Csacvir, Csacrunt,andCsacdsxF were more highly expressed in female. In addition, Csactra2 and CsacgroX9 were enriched in Spliceosome, Notch signaling, and Wnt signaling pathways, respectively. And the pathways are crucial in regulating insect growth, differentiation and development. This transcriptome study provides rich and significant information regarding the genes involved in sex differentiation and determination, which would improve our understanding of the molecular mechanisms related to sex determination and be helpful for providing the basis for a wide spectrum of strategies to benefit pest control and prevention, and agriculture and public health
Liu Jianbai,Yi Jiequn,Wu Han,Lu Yinglin,Mao Yongkai,Lin Mingjiang,Li Jihu,An Yuxing 한국곤충학회 2022 Entomological Research Vol.52 No.9
Chilo sacchariphagus Bojeris is one of the most dangerous pests of sugarcane. The larvae damage the seedlings and stems of sugarcane and also harm sorghum, corn and other crops, which causes great economic losses to the sugar industry every year. Transcriptome sequencing and expression profile analysis of cytochrome P450 monooxygenase (CYP), carboxylesterase and glutathione S-transferase (GST) were carried out, which could provide a basis for drug resistance monitoring and drug resistance management of the pest. Unigenes of C. sacchariphagus were obtained by using the Illumina HiSeqTM 4,000 platform as 150 bp paired-end reads. A total of 173,013 unigenes were obtained after data assembly and redundancy removal. 28,330 unigenes were annotated based on multiple public databases, and the number of unigenes annotated by NR database was the largest. According to the transcriptome analysis, 214 candidate detoxification enzyme genes were identified, including 44 GSTs,138CYPs, and 32 CarEs. Phylogenetic analysis showed that the CYPs were mainly clustered in CYP4, CYP6, CYP9 and CYP12 subfamilies; CarEs mainly include antennal CarEs, venom CarEs and CarEs NOTUM; while the GSTs cluster mainly contains subfamilies such as delta, omega, epsilon, theta, zeta and sigma. In this study, transcriptome information of C. sacchariphagus was obtained, and genes related to detoxification were identified, which could provide data and a basis for the further study of detoxification and host plant adaptation mechanism of C. sacchariphagus.
Mingfei Liu,Junli Wang,Birui Tian,Jingjing Huang,Rongrong Zhang,Yuxing Lin,Zefeng Xiao 한국원예학회 2017 원예과학기술지 Vol.35 No.2
Tea is one of the most consumed beverages worldwide and the relatively high levels polyphenols is benefit for health. In this study, we developed an efficient system for proliferation of callus from ‘Anji Baicha’, a cultivar of tea (Camellia sinensis ). Callus tissue was initially induced by culturing leaf explants on medium containing different plant growth regulators. For callus induction, thidiazuron (TDZ) was more effective than 2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthalene acetic acid (NAA), and N6-benzyladenine (BA). The frequency of callus induction from leaf explants reached 90.21% on 1.0 mg·L<SUP>-1</SUP> TDZ and the developed callus was reddish and friable. We also tested the effect of different concentrations of NAA, 2,4-D, indole 3-acetic acid (IAA), BA, and TDZ, alone and in combinations, on callus proliferation. Medium supplemented with TDZ in combination with IAA was suitable for callus proliferation and accumulation of tea polyphenols. The growth index value and tea polyphenol content of callus cultured on MS medium containing 0.5 mg·L<SUP>-1</SUP> TDZ and 1.0 mg·L<SUP>-1</SUP> IAA was maximally 1,351% and 23.24%, respectively, and the relative abundance of epicatechin was as high as 17.449%. We also measured the antioxidant activity of all samples and the callus with the highest tea polyphenol content also exhibited high potential radical scavenging activity.
Microwave Sintering of Graphene-Nanoplatelet-Reinforced Al<sub>2</sub>O<sub>3</sub>-based Composites
Ai, Yunlong,Liu, Ying,Zhang, Qiuyu,Gong, Yuxing,He, Wen,Zhang, Jianjun The Korean Ceramic Society 2018 한국세라믹학회지 Vol.55 No.6
In this study, we performed a microwave sintering (MWS) of $Al_2O_3$ ceramic and $Al_2O_3$-based composites with nominal contents of graphene nanoplatelets (GPLs) of 0.2, 0.4, 0.6, and 0.8 vol%. The GPL dispersion in N-methyl pyrroleketone was optimized to deagglomerate the GPLs without damaging their structure. Dense composites were then obtained by MWS at $1500^{\circ}C$ for 30 min. The effects of different GPL contents on the phase compositions, microstructures, and mechanical properties of the composites were investigated. The microstructures of the composites became finer with the incorporation of the GPLs. The well-dispersed GPL fillers led to higher sintered densities in the composites. The optimal mechanical properties were achieved with 0.4 vol% GPLs. For this sample, the hardness, fracture toughness, and bending strength were $2000kgf/mm^2$, $6.19MPa{\cdot}m^{1/2}$, and 365.10 MPa, respectively. The addition of GPL could improve the microstructure of the $Al_2O_3$ ceramic and has potential to improve the fracture toughness of the ceramics.
Ma Yanyan,Zhang Weida,CHENGSHAOBO,Yang Wanting,Liu Yuxing,Yang Shengyu,Zhang Xinling,Guo Minrui,Chen Guogang 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.6
Prunus domestica L. cv. Ximei fruit perishes quickly due to intense metabolic activity after being harvested. To prolong shelf life and maintain fruit quality, the eff ects of 1-methylcyclopropene (1-MCP) treatment on P. domestica fruit during storage at 4 ± 1 °C were investigated. The results showed that the soluble solid content (SSC), respiratory rate (29.8%), ethylene production (27.2%), anthocyanin content, malonaldehyde content (MDA), hydrogen peroxide content (H 2O2), and superoxide anion activity (O 2·−) of P. domestica fruit were all signifi cantly reduced by 1-MCP treatment (1.0 μL L −1), while the content of ascorbic acid and total phenol, and the activity of SUPEROXIDE DISMUTASE (SOD, 61.3%), CATALASE (CAT, 39.0%), ASCORBATE PEROXIDASE (APX, 23.7%), and PEROXIDASE (POD, 38.0%) increased compared to untreated fruit after 35 days of cold storage. Overall, 1-MCP treatment could maintain high postharvest quality and anti- oxidant activity in P. domestica fruit