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Lu, M.,Chang, Z.,Bae, M.J.,Oh, S.M.,Chung, K.H.,Park, J.S. Academic Press 2013 Fish & shellfish immunology Vol.35 No.2
In bony fish or other aquatic vertebrates, the aryl hydrocarbon receptor (AhR) signaling pathway is initiated by exposure to polycyclic (or/and halogenated) aromatic hydrocarbons (PAHs, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin, TCDD), which subsequently induces the up-regulated expression of a series of related genes (such as cytochrome P4501A (CYP1A)). However, a lack of applicable protein reagents hinders our further understanding of the AhR signaling pathway, which focuses only on gene-based investigations. The goldfish (Carassius auratus) is an ideal model for a study of environmental pollution in whole-Asian fresh water. Here, three sensitive and specific polyclonal antisera against goldfish AhR1, AhR2, and CYP1A proteins were developed. These antisera not only bound the in-vitro synthesized target proteins, but recognized the real proteins expressed in goldfish tissues, with minimal cross-reactivity to non-specific proteins. Together with the analysis of semi-quantitative RT-PCR and polyclonal-antibody-based sandwich ELISA, we confirmed that goldfish AhRs differed in the expression (mRNA and protein levels) patterns among test tissues. Importantly, the relative abundance of each AhR mRNA levels from the different tissues showed no obvious consistency with their protein levels. After exposure to TCDD, goldfish AhR2 showed a more sensitivity than AhR1, and stimulated CYP1A expression directly, similar with the other reported fish models. Overall, development of these antibodies in this study will allow valuable and versatile investigations to further understand the AhR signaling pathway, and different expression (mRNA and protein) patterns represent the first step in determining the regulatory mechanisms underlying the TCDD-exposed aquatic environment.
Chinese speakers perceptibility of the Korean voiceless obstruents
Ah-Lum Kwon 한국언어연구학회 2014 언어학연구 Vol.19 No.3
Kwon, Ah-Lum. 2014. Chinese speakers perceptibility of the Korean voiceless obstruents. Journal of Linguistic Studies 19(3), 233-253. One characteristic of Korean consonants is that there are three distinctive voiceless sounds in the stop category. Since Chinese has two-way contrast stop consonants while Korean has three-way contrast stop consonants, it was anticipated that the Chinese group selected for the experiment would demonstrate particular difficulties in learning a foreign language with a different system of contrast consonants. This paper suggests factors in two aspects as major factors in distinguishing the three-way contrast in the Korean voiceless obstruents. The first aspect is speech production, including VOT (Voice Onset Time) and F0 (fundamental frequency). The second aspect is speech perception, including the factor of the pronunciation of Korean learners. Generalizing from the results of Perception Test 1 and 2, adult learners of a foreign language seem to exhibit a correlation between articulation and auditory perception. Perception Test 3 shows how both Korean and Chinese participants can use F0 and VOT values as the major factors in distinguishing three-way contrast stop consonants in Korean. (Kyungpook National University)
Hydrogen Treatment Protects against Cell Death and Senescence Induced by Oxidative Damage
( A Lum Han ),( Seong-hoon Park ),( Mi Sung Park ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.2
Hydrogen has potential for preventive and therapeutic applications as an antioxidant. However, micro- and macroparticles of hydrogen in water disappear easily over time. In order to eliminate reactive oxygen species (ROS) related with the aging process, we used functional water containing nanoparticle hydrogen. Nanoparticle hydrogen does not disappear easily and collapse under water after long periods of time. We used murine embryonic fibroblasts that were isolated from 12.5-day embryos of C57BL/6 mice. We investigated the ability of nanoparticle hydrogen in water to suppress hydroxyurea-induced ROS production, cytotoxicity, and the accumulation of β-galactosidase (an indicator of aging), and promote cell proliferation. The accumulation of β-galactosidase in the cytoplasm and the appearance of abnormal nuclei were inhibited by daily treatment of cells with hydrogen water. When the aging process was accelerated by hydroxyurea-induced oxidative stress, the effect of hydrogen water was even more remarkable. Thus, this study showed the antioxidant and antisenescence effects of hydrogen water. Nanoparticle hydrogen water is potentially a potent anti-aging agent.
영화 속 여배우 캐릭터 이미지 특징에 따른 미용요소 연구 -여배우 전도연이 출연한 작품을 중심으로-
한아름 ( Ah-lum Han ),이정민 ( Jeoung-min Lee ) 한국미용예술경영학회 2012 미용예술경영연구 Vol.6 No.1
This research has examined Jeon do-yeon' s characters and characteristic changes on her roles in each film by analyzing image making factors dividing sectors such as makeup, hair etc. screened from 1997 to 2011 referredto preceding paper, professional books, journals, books, news, internet, etc. As a result, Actress’ s make-up of beauty elements is determined by the occupation or personality of the character, whilst beauty elements in the movie provides a clue to predict the plot and even main actor/actress' s profession, psychological state, and characteristic of the age. As the fact of the limitation that by analyzing a person’ s work cannot be assigned to all the film, more substantive and analytical studies may be needed.