Expression of Arabidopsis HOMEODOMAIN GLABROUS 11 Enhances Tolerance to Drought Stress in Transgenic Sweet Potato Plants

Long Ruan,Lijuan Chen,Yihong Chen,Jinling He,Wei Zhang,Zhengliang Gao,Yunhua Zhang 한국식물학회 2012 Journal of Plant Biology Vol.55 No.2

Sweet potato (Ipomoea batatas L. cv. Lizixiang)is a nutritious arable crop with a low drought tolerance during growth and maturation. The Arabidopsis HOMEODOMAIN GLABROUS 11 (HDG11) gene can increase drought tolerance in tobacco and tall fescue plants. To determine the effect of HDG11 in the sweet potato, transgenic plants that expressed the HDG11 gene were generated by Agrobacterium-mediated transformation. Expression of the transgene was confirmed using Southern blotting, reverse transcription-polymerase chain reaction (RT-PCR) and DNA sequencing. Two independent HDG11 transgenic lines were evaluated and increased drought stress tolerance was observed in both lines, compared to wild-type (WT) plants. Under drought stress conditions, net photosynthesis rate (Pn), the efficiency of excitation energy captured by open PSII reaction centers (Fv/Fm) and water use efficiency (WUE) increased, and transpiration rate (Tr) decreased in HDG11 transgenic plants compared to WT. HDG11 transgenic plants also had decreased lipid membrane oxidative damage, reduced H2O2 accumulation and increased ROSscavenging enzyme activity during drought stress treatment. This study indicates that overexpression of the Arabidopsis HDG11 gene improved drought tolerance in the sweet potato.

A new cable force identification method considering cable flexural rigidity

Long Wang,Bo Wu,Junyue Gao,Kairong Shi,Wenzhi Pan,Zhuoyi He,Zhijian Ruan,Quanpan Lin 국제구조공학회 2018 Structural Engineering and Mechanics, An Int'l Jou Vol.68 No.2

Cables are the main load-bearing members of prestressed structure and other tensegrity structures. Based on the static equilibrium principle, a new cable force identification method considering cable flexural rigidity is proposed. Its computational formula is derived and the strategy to solve its implicit formula is introduced as well. In order to improve the reliability and practicality of this method, the influence of the cable flexural rigidity on cable force identification accuracy is also investigated. Through cable force identification experiments, the relationships among certain parameters including jacking force, jacking displacement, initial cable force, and sectional area (flexural rigidity) are studied. The results show that the cable force calculated by the proposed method considering flexural rigidity is in good agreement with the finite element results and experimental results. The proposed method with high computational accuracy and resolution efficiency can avoid the influences of the boundary condition and the length of the cable on calculation accuracy and is proven to be conveniently applied to cable force identification in practice.

A new cable force identification method considering cable flexural rigidity

Wang, Long,Wu, Bo,Gao, Junyue,Shi, Kairong,Pan, Wenzhi,He, Zhuoyi,Ruan, Zhijian,Lin, Quanpan 국제구조공학회 2018 Structural Engineering and Mechanics, An Int'l Jou Vol.68 No.2

Cables are the main load-bearing members of prestressed structure and other tensegrity structures. Based on the static equilibrium principle, a new cable force identification method considering cable flexural rigidity is proposed. Its computational formula is derived and the strategy to solve its implicit formula is introduced as well. In order to improve the reliability and practicality of this method, the influence of the cable flexural rigidity on cable force identification accuracy is also investigated. Through cable force identification experiments, the relationships among certain parameters including jacking force, jacking displacement, initial cable force, and sectional area (flexural rigidity) are studied. The results show that the cable force calculated by the proposed method considering flexural rigidity is in good agreement with the finite element results and experimental results. The proposed method with high computational accuracy and resolution efficiency can avoid the influences of the boundary condition and the length of the cable on calculation accuracy and is proven to be conveniently applied to cable force identification in practice.

Heavy concrete shielding properties for carbon therapy

Jin-Long Wang,Jiade J Lu,Da-Jun Ding,Wen-Hua Jiang,Ya-Dong Li,Rui Qiu,Hui Zhang,Xiao-Zhong Wang,Huo-Sheng Ruan,Yan-Bing Teng,Xiao-Guang Wu,Yun Zheng,Zi-Hao Zhao,Kai-Zhong Liao,Huan-Cheng Mai,Xiao-Dong Korean Nuclear Society 2023 Nuclear Engineering and Technology Vol.55 No.6

As medical facilities are usually built at urban areas, special concrete aggregates and evaluation methods are needed to optimize the design of concrete walls by balancing density, thickness, material composition, cost, and other factors. Carbon treatment rooms require a high radiation shielding requirement, as the neutron yield from carbon therapy is much higher than the neutron yield of protons. In this case study, the maximum carbon energy is 430 MeV/u and the maximum current is 0.27 nA from a hybrid particle therapy system. Hospital or facility construction should consider this requirement to design a special heavy concrete. In this work, magnetite is adopted as the major aggregate. Density is determined mainly by the major aggregate content of magnetite, and a heavy concrete test block was constructed for structural tests. The compressive strength is 35.7 MPa. The density ranges from 3.65 g/cm³ to 4.14 g/cm³, and the iron mass content ranges from 53.78% to 60.38% from the 12 cored sample measurements. It was found that there is a linear relationship between density and iron content, and mixing impurities should be the major reason leading to the nonuniform element and density distribution. The effect of this nonuniformity on radiation shielding properties for a carbon treatment room is investigated by three groups of Monte Carlo simulations. Higher density dominates to reduce shielding thickness. However, a higher content of high-Z elements will weaken the shielding strength, especially at a lower dose rate threshold and vice versa. The weakened side effect of a high iron content on the shielding property is obvious at 2.5 µSv=h. Therefore, we should not blindly pursue high Z content in engineering. If the thickness is constrained to 2 m, then the density can be reduced to 3.3 g/cm³, which will save cost by reducing the magnetite composition with 50.44% iron content. If a higher density of 3.9 g/cm³ with 57.65% iron content is selected for construction, then the thickness of the wall can be reduced to 174.2 cm, which will save space for equipment installation.

Development of TREND dynamics code for molten salt reactors

Yu, Wen,Ruan, Jian,He, Long,Kendrick, James,Zou, Yang,Xu, Hongjie Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.2

The Molten Salt Reactor (MSR), one of the six advanced reactor types of the 4th generation nuclear energy systems, has many impressive features including economic advantages, inherent safety and nuclear non-proliferation. This paper introduces a system analysis code named TREND, which is developed and used for the steady and transient simulation of MSRs. The TREND code calculates the distributions of pressure, velocity and temperature of single-phase flows by solving the conservation equations of mass, momentum and energy, along with a fluid state equation. Heat structures coupled with the fluid dynamics model is sufficient to meet the demands of modeling MSR system-level thermal-hydraulics. The core power is based on the point reactor neutron kinetics model calculated by the typical Runge-Kutta method. An incremental PID controller is inserted to adjust the operation behaviors. The verification and validation of the TREND code have been carried out in two aspects: detailed code-to-code comparison with established thermal-hydraulic system codes such as RELAP5, and validation with the experimental data from MSRE and the CIET facility (the University of California, Berkeley's Compact Integral Effects Test facility).The results indicate that TREND can be used in analyzing the transient behaviors of MSRs and will be improved by validating with more experimental results with the support of SINAP.

Lycorine: A Potential Broad-Spectrum Agent Against Crop Pathogenic Fungi

( Jin Wen Shen ),( Yuan Ruan ),( Wei Ren ),( Bing Ji Ma ),( Xiao Long Wang ),( Chun Feng Zheng ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.3

A screening test showed that lycorine exhibited significant antifungal activity against 24 pathogenic crop fungi at concentrations of 500μg/ml and 100μg/ml, respectively. Fusarium graminearum was selected for antifungal mechanism studies by observing its mycelial morphology and investigating the variations in its conductivity. In addition, the substance absorption and metabolism of F. graminearum were explored. The mechanism was revealed as being one by which lycorine destroyed the cellular membrane and further influenced substance absorption and cell metabolism.

Horizon supertranslation and degenerate black hole solutions

Cai, Rong-Gen,Ruan, Shan-Ming,Zhang, Yun-Long Institute of Physics Pub 2016 The journal of high energy physics Vol.2016 No.9

Study on the Joining of 2D Microstructure during the Fabrication of 3D Micro-Mold

Bin Xu,Xiao-yu Wu,Jian-guo Lei,Feng Luo,Chen-lin Du,Shuang-chen Ruan,Zhen-long Wang 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.

The 3D micro-mold which is fabricated by the femtosecond laser cutting and micro electric resistance slip welding is formed throughthe lamination of multilayer 2D microstructures. By using this technology, the 3D micro-mold with a high depth-width ratio can bemanufactured. In order to improve the laminated precision and joining strength of each layer of 2D microstructure, this researchapplied layer-by-layer micro electric resistance slip welding to weld each layer of 2D microstructure. Firstly, the proper technicalparameters were obtained through the experiments of layer-by-layer micro electric resistance slip welding. Secondly, through thelayer-by-layer micro electric resistance slip welding, multilayer 2D microstructures were weld together and a 3D micro-mold wasformed. Moreover, the anti-shear stress test of 3D micro-mold was done. In the layer-by-layer slip welding process, electrode couldproduce some losses and the losses could deposit on the surface of micro-mold. This research also studied the deposition effect ofthe electrode. Finally, based on the above studies, the micro square-hole array and the micro-gear cavity were fabricated by joiningmultilayer 2D microstructure.