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Chun-Yeung Lo,Sze-Ting Choi,Olive Tin-Wai Li,Jacky Chi-Ki Ngo,David Chi-Cheong Wan,Leo Lit-Man Poon,Pang-Chui Shaw1 한국구조생물학회 2015 Biodesign Vol.3 No.3
Currently, many strains of influenza A virus have developed resistance against anti-influenza drugs, and it is essential to find new chemicals to combat this virus. The viral nucleoprotein (NP) is a major component of the ribonucleoprotein (RNP) complex for the transcription and replication of the virus. In this study, we have employed surface plasmon resonance direct binding screening on the influenza A NP and found a hit compound 16 that can subdue influenza RNP activities. Subsequently, two analogs (compounds 55 & 58) from compound 16 were identified which inhibit RNP activities of various influenza A subtypes and viral growth at micromolar levels. These analogs were also shown to directly interact with NP, with KD 12.0±1.25 and 41.6±1.93 μM respectively by surface plasmon resonance assay.
Sensitive detection of human fucosidase by an activity-based probe
Manjula Nandakumar,Lo-Chun,Lee-Chiang Lo,Chun Hung Lin 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
Alpha-L-fucosidase catalyzes the removal of L-Fucose residues from the non-reducing end of glycoconjugates. There are two fucosidases in human, including α-L-fucosidase-1 (FUCA1) and α-L-fucosidase-2 (FUCA2). Abnormal fucosidase activity has been associated with many diseases, such as fucosidosis (one of the lysosomal storage disorders), hepatocellular cancer and breast cancer. We previously demonstrated that gastric epithelial cells secrete FUCA2 extracellularly upon the infection of Helicobacter pylori, a strategy tomodify the cell surface glycoconjugates to prevent the bacterial adhesion. However the role and physiological function of FUCA2 still remains ambiguous, not mentioning the difficulty that the enzyme is highly unstable and the activity is often too small for detection. We developed an activity-based probe for sensitive detection of the enzyme activity in vitro and in vivo. First of all, the probe was utilized for dot-blot assay to measure the H. pylori-induced fucosidase activity in a quantitative manner. Secondly, the probe provided a real-time detection of the increased fucosidase activity by using confocal microscopy. The result also supported the idea that the bacterial infection not only leads to an increase in the extracellular activity of FUCA2, but also an intracellular increase of fucosidase activity. Western blotting analysis by FUCA1- and FUCA2-specific antibodies further corroborated the aforementioned increase levels of FUCA1 and FUCA2 activities, respectively. Our current emphasis is placed to search for the factors of H. pylori in association with the secretion of fucosidase during infection.
Wei Chun Wang,Bodil Rasmussen,Anthony Lin Zhang,Li-Wei Lin,Trisha Dunning,강승완,Byung-Joo Park,Sing Kai Lo 사단법인약침학회 2009 Journal of Acupuncture & Meridian Studies Vol.2 No.3
Objective: This systematic review aimed to critically appraise published clinical trials designed to assess the effect of Tai Chi on psychosocial well-being. Data Sources: Databases searched included MEDLINE, CINAHL, EMBASE, HEALT, PsycINFO, CISCOM, the Cochrane Central Register of Controlled Trials of the Cochrane Library, and dissertations and conference proceedings from inception to August 2008. Review Methods: Methodological quality was assessed using a modified Jadad scale. A total of 15 studies met the inclusion criteria (i.e. English publications of randomized controlled trials with Tai Chi as an intervention and psychological well-being as an outcome measure), of which eight were high quality trials. The psychosocial outcomes measured included anxiety (eight studies), depression (eight studies), mood (four studies), stress (two studies), general mental health three studies), anger, positive and negative effect, self-esteem, life satisfaction, social interaction and self-rated health (one study each). Results: Tai Chi intervention was found to have a significant effect in 13 studies, especially in the management of depression and anxiety. Although the results seemed to suggest Tai Chi is effective, they should be interpreted cautiously as the quality of the trials varied substantially. Furthermore, significant findings were shown in only six high quality studies. Moreover, significant between group differences after Tai Chi intervention was demonstrated in only one high quality study (the other three significant results were observed in non-high quality studies). Two high quality studies in fact found no significant Tai Chi effects. Conclusion: It is still premature to make any conclusive remarks on the effect of Tai Chi on psychosocial well-being. Objective: This systematic review aimed to critically appraise published clinical trials designed to assess the effect of Tai Chi on psychosocial well-being. Data Sources: Databases searched included MEDLINE, CINAHL, EMBASE, HEALT, PsycINFO, CISCOM, the Cochrane Central Register of Controlled Trials of the Cochrane Library, and dissertations and conference proceedings from inception to August 2008. Review Methods: Methodological quality was assessed using a modified Jadad scale. A total of 15 studies met the inclusion criteria (i.e. English publications of randomized controlled trials with Tai Chi as an intervention and psychological well-being as an outcome measure), of which eight were high quality trials. The psychosocial outcomes measured included anxiety (eight studies), depression (eight studies), mood (four studies), stress (two studies), general mental health three studies), anger, positive and negative effect, self-esteem, life satisfaction, social interaction and self-rated health (one study each). Results: Tai Chi intervention was found to have a significant effect in 13 studies, especially in the management of depression and anxiety. Although the results seemed to suggest Tai Chi is effective, they should be interpreted cautiously as the quality of the trials varied substantially. Furthermore, significant findings were shown in only six high quality studies. Moreover, significant between group differences after Tai Chi intervention was demonstrated in only one high quality study (the other three significant results were observed in non-high quality studies). Two high quality studies in fact found no significant Tai Chi effects. Conclusion: It is still premature to make any conclusive remarks on the effect of Tai Chi on psychosocial well-being.