일본잎갈나무 낙엽의 추출성분 및 항산화활성

사전령,권동주,김진규,황병호,배영수 江原大學校 森林科學硏究所 2005 Journal of Forest Science Vol.21 No.-

일본잎갈나무 낙엽 (8.5kg)을 채취하여 9.5% EtOH 용액으로 추출하고 농축된 추출용액은 분획깔때기로 헥산, 메틸렌클로라이드, 에틸아세테이트 및 수용성으로 순차 추출하여 동결건조하였다. 에틸아세테이트용성과 수용성 분획에 대하여 칼럼크로마트크래피를 실시하였고, 충진물질로는 Sephadex LH-20을, 용리용매로는 메탄올 수용액 및 에탈올-핵산 혼합용액을 사용하였다. 단리된 화합물들은 TLC로 확인한 후 NMR 스펙트럼을 사용하여 정확한 구조규명을 하였고 FAB-MS와 EI-MS 스펙트럼으로 분자량을 측정하였다. 일본잎갈나무 낙엽으로부터 5개의 화합물을 단리하였으며, 각 분획물과 단리된 화합물들은 DPPH 라디칼 소거법을 이용하여 항산화 시험을 실시하였다. Fallen neeldes (8.5kg) of Larix kaempferi were separately collected. extracted with 95% EtOH. EtOH extract was evaporated under reduced pressure, concentrated then successively fractionated with a series of hexane. methylene chloride, ethylaectate and water on a separatory funnel. Then, each fraction was freeze dried. A portion of ethylacetate and water soluble powder were packed on a column chromatography (Sephadex LH-20) eluting with aqueous MeOH and EtOH-hexane mixture. Spectrometric analyses such as NMR and FAB-MS including TLC were performed to characterize the structures of isolated compounds.

오동나무 잎의 페놀성 화합물

사전령,김진규,권동주,배영수 江原大學校 森林科學硏究所 2005 Journal of Forest Science Vol.21 No.-

강원대학교 구내림에서 오동나무 잎을 채취하여 아세톤-물(7:3, v/v)로 추출하고 n-hexane, methylene chloride, ethylacetate 및 H_(2)O 등 네 개의 분획으로 분리한 후 에틸아세테트용성 분획물을 Sephadex LH-20으로 칼럼 크로마토그래피를 수행하였으며 용리용매는 메탄올 수용액과 에탄올-핵산 혼합액을 사용하였다. 그 결과 flavonoid류 화합물인( +)-catechin, ( - )-epicatechin, apigenin, luteolin, taxifolin과 phenolic acid류 화합물인 p-hydroxybenzoic acid, vanillic acid 및 p-coumaric acid를 단리 하였다. 단리된 화합물은 NMR 및 MS 스펙트럼을 이용하여 구조를 결정하였다. The leaves of Paulownia Coreana Uyeki were collected, extracted with acetone-H_(2)O(7:3, v/v), fractionated with n-hexane, methylene chloride and ethylacetate, and freeze dried to give some dark brown powder. The ethylacetate soluble mixture was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol and ethanol-hexane mixture as eluents. Spectrometric analysis such as NMR and MS From the ethylacetate fraction, five flavonides and three phenolic acids were isolated and determined.

Pathogenesis, Early Diagnosis, and Therapeutic Management of Alcoholic Liver Disease

Kong, Ling-Zu,Chandimali, Nisansala,Han, Ying-Hao,Lee, Dong-Ho,Kim, Ji-Su,Kim, Sun-Uk,Kim, Tae-Don,Jeong, Dong Kee,Sun, Hu-Nan,Lee, Dong Sun,Kwon, Taeho MDPI AG 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.11

<P>Alcoholic liver disease (ALD) refers to the damages to the liver and its functions due to alcohol overconsumption. It consists of fatty liver/steatosis, alcoholic hepatitis, steatohepatitis, chronic hepatitis with liver fibrosis or cirrhosis, and hepatocellular carcinoma. However, the mechanisms behind the pathogenesis of alcoholic liver disease are extremely complicated due to the involvement of immune cells, adipose tissues, and genetic diversity. Clinically, the diagnosis of ALD is not yet well developed. Therefore, the number of patients in advanced stages has increased due to the failure of proper early detection and treatment. At present, abstinence and nutritional therapy remain the conventional therapeutic interventions for ALD. Moreover, the therapies which target the TNF receptor superfamily, hormones, antioxidant signals, and MicroRNAs are used as treatments for ALD. In particular, mesenchymal stem cells (MSCs) are gaining attention as a potential therapeutic target of ALD. Therefore, in this review, we have summarized the current understandings of the pathogenesis and diagnosis of ALD. Moreover, we also discuss the various existing treatment strategies while focusing on promising therapeutic approaches for ALD.</P>

Influence of Atmospheric Relative Humidity on Ultraviolet Flux and Aerosol Direct Radiative Forcing: Observation and Simulation

Dong Xia,Ling Chen,Huizhong Chen,Xuyu Luo,Tao Deng 한국기상학회 2016 Asia-Pacific Journal of Atmospheric Sciences Vol.52 No.4

The atmospheric aerosols can absorb moisture from the environment due to their hydrophilicity and thus affect atmospheric radiation fluxes. In this article, the ultraviolet radiation and relative humidity (RH) data from ground observations and a radiative transfer model were used to examine the influence of RH on ultraviolet radiation flux and aerosol direct radiative forcing under the clear-sky conditions. The results show that RH has a significant influence on ultraviolet radiation because of aerosol hygroscopicity. The relationship between attenuation rate and RH can be fitted logarithmically and all of the R2 of the 4 sets of samples are high, i.e. 0.87, 0.96, 0.9, and 0.9, respectively. When the RH is 60%, 70%, 80% and 90%, the mean aerosol direct radiative forcing in ultraviolet is −4.22W m−2, −4.5W m−2, −4.82W m−2 and −5.4W m−2, respectively. For the selected polluted air samples the growth factor for computing aerosol direct radiative forcing in the ultraviolet for the RH of 80% varies from 1.19 to 1.53, with an average of 1.31.

Bioavailability of Salvianolic Acid B and Effect on Blood Viscosities after Oral Administration of Salvianolic Acids in Beagle Dogs

Dong-Yan Gao,Li-Mei Han,Li-Hong Zhang,Xiao-Ling Fang,Jian-Xin Wang 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.5

Salvianolic acid B (SalB) is an active component isolated from Chinese herbal medicine Salvia miltiorrhiza. The aim of this study was to investigate the extent of absolute oral bioavailability (F) of SalB in beagle dogs and the effect on blood viscosity after intravenous and oral administration of Salvianolic acids (SAs). A gradient elution HPLC method was developed and validated to determine the concentration of SalB and its three possible metabolites in plasma. After SAs (180 mg/kg, p.o.; 9 mg/kg, i.v.) were given, the AUCs of SalB were 1680 ± 670 and 7840 ± 1140 ng/mL·h, respectively. The F of SalB in dogs was calculated to be only 1.07 ± 0.43%. The blood viscosity was remarkably decreased after a single intravenous injection of SAs (9 mg/kg). However, no significant change of blood viscosity was observed after a single oral administration of SAs (180 mg/kg). The results suggested that the F of SalB was extremely low and single oral administrated SAs had no effect on ameliorating blood viscosity in beagle dogs.

Cr(VI) Resistance and Removal by Indigenous Bacteria Isolated from Chromium-Contaminated Soil

( Dong Yan Long ),( Xian Jin Tang ),( Kuan Cai ),( Guang Cun Chen ),( Chao Feng Shen ),( Ji Yan Shi ),( Ling Gui Chen ),( Ying Xu Chen ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8

The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing ability, with 100 mg/l Cr(VI) being completely removed within 144 h. It could effectively remove Cr(VI) over a wide pH range from 5.5 to 9.5, with the optimal pH of 8.5. The amount of Cr(VI) removed increased with initial Cr(VI) concentration. Data from the time-course analysis of Cr(VI) removal by strain LY6 followed first-order kinetics. Based on the 16S rRNA gene sequence, strain LY6 was identified as Pseudochrobactrum asaccharolyticum, a species that had never been reported for Cr(VI) removal before. Transmission electron microscopy and energy dispersive X-ray spectroscopy analysis further confirmed that strain LY6 could accumulate chromium within the cell while conducting Cr(VI) removal. The results suggested that the indigenous bacterial strain LY6 would be a new candidate for potential application in Cr(VI) pollution bioremediation.

Effect of Waste Tire Rubber Particles on Concrete Abrasion Resistance Under High-Speed Water Flow

Ling-Yun Feng,Ai-Jiu Chen,Han-Dong Liu 한국콘크리트학회 2021 International Journal of Concrete Structures and M Vol.15 No.5

Rubberized concrete is an environmentally friendly building material that mixes rubber particles from old automobile tires into normal concrete in place of fine aggregate. The addition of rubber particles can improve the abrasion resistance of normal concrete observably. It has a good application prospect in hydraulic engineering, especially in the concrete building parts with high abrasion resistance. However, there are few experimental studies on the abrasion resistance of rubberized concrete, and the influence law and mechanism of rubber particles on the abrasion resistance of concrete are not understood. In this paper, the abrasion resistance of rubberized concrete is studied using the underwater-steel-ball method. The results show that rubber particles increase the slump of concrete mixtures. The abrasion resistance of rubberized concrete increases significantly with increasing rubber particle content, whereas the compressive strength decreases linearly. For the same rubber particle size and content, the abrasion resistance of rubberized concrete positively correlates with compressive strength and larger rubber particles significantly improve the abrasion resistance. Rubber particle content is the factor that most strongly affects abrasion resistance of rubberized concrete, followed by the compressive strength. Rubber particle pretreatment methods of NaOH + KH570 can significantly improve the abrasion resistance of rubberized concrete.

Case Reports : Sturge-Weber Syndrome

( Ling Chen ),( Jin Jin Wu ),( Min Hui Xu ),( Nian Chen ),( Ya Dong Yang ) 대한피부과학회 2011 Annals of Dermatology Vol.23 No.4

Sturge-Weber syndrome (SWS) is a neurocutaneous syndrome, characterized by the association of facial port-wine hemangiomas in the trigeminal nerve distribution area, with vascular malformation(s) of the brain (leptomeningeal angioma) with or without glaucoma. Herein, we reported Sturge-Weber syndrome in a 50-year-old man, who presented port-wine hemangiomas and epilepsy. In this case, the patient`s epilepsy episodes from his first year of life had been ignored and separated from the entity of SWS by his physicians, which led to delayed treatment. This case illustrates the importance of careful examination of patients of any age with hemangiomas in the trigeminal nerve with concomitant episodes of epilepsy. In such cases, there should be yearly neuroimaging screenings to guaranteed early interdisciplinary interventions from the time of definite diagnosis. (Ann Dermatol 23(4) 551~553, 2011)

Part 1. Trade And Industrial Development : 2. Analysis and Evaluation on Institutional Factors of Trade in Northeast Asia: Viewpoints from Chinese Import and Export Specialists

( Ling Xiao Dong ) 연세대학교 동서문제연구원 1996 East and West Studies Series Vol.40 No.-

Rheological Phase Synthesis of Er-Doped LiV3O8 as Electroactive Material for a Cathode of Secondary Lithium Storage

Ling-Ling Xie,Yuan-Dong Xu,Jie-Jie Zhang,Cheng-Peng Zhang,Xiao-Yu Cao,Ling-Bo Qu 대한금속·재료학회 2013 ELECTRONIC MATERIALS LETTERS Vol.9 No.4

Er-doped LiV3O8 as cathode material for secondary lithium batteries was prepared through a rheological phase reaction method. The as-prepared materials were investigated by x-ray diffraction (XRD), scanning electron microscopy (SEM), and galvanostatic discharge/charge measurements. The results indicate that Er doped phase preserves the layered structure of the pristine LiV3O8 and has an enlarged interlayer spacing. Compared to LiV3O8 sample, Er-doped LiV3O8 sample displays more uniform particles and large surface area. The electrochemical test shows that Er doping does not change the process of Li+ insertion/deinsertion. Er-doped LiV3O8 electrode exhibits an initial discharge capacity of 294.2 mAh g−1 and maintains a stable capacity of 220.7 mAh g−1 after 50 cycles, indicating a greatly improved good cycleability comparing with the undoped one.