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Pathogenesis, Early Diagnosis, and Therapeutic Management of Alcoholic Liver Disease
Kong, Ling-Zu,Chandimali, Nisansala,Han, Ying-Hao,Lee, Dong-Ho,Kim, Ji-Su,Kim, Sun-Uk,Kim, Tae-Don,Jeong, Dong Kee,Sun, Hu-Nan,Lee, Dong Sun,Kwon, Taeho MDPI AG 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.11
<P>Alcoholic liver disease (ALD) refers to the damages to the liver and its functions due to alcohol overconsumption. It consists of fatty liver/steatosis, alcoholic hepatitis, steatohepatitis, chronic hepatitis with liver fibrosis or cirrhosis, and hepatocellular carcinoma. However, the mechanisms behind the pathogenesis of alcoholic liver disease are extremely complicated due to the involvement of immune cells, adipose tissues, and genetic diversity. Clinically, the diagnosis of ALD is not yet well developed. Therefore, the number of patients in advanced stages has increased due to the failure of proper early detection and treatment. At present, abstinence and nutritional therapy remain the conventional therapeutic interventions for ALD. Moreover, the therapies which target the TNF receptor superfamily, hormones, antioxidant signals, and MicroRNAs are used as treatments for ALD. In particular, mesenchymal stem cells (MSCs) are gaining attention as a potential therapeutic target of ALD. Therefore, in this review, we have summarized the current understandings of the pathogenesis and diagnosis of ALD. Moreover, we also discuss the various existing treatment strategies while focusing on promising therapeutic approaches for ALD.</P>
Huynh, Do Luong,Zhang, Jiao Jiao,Chandimali, Nisansala,Ghosh, Mrinmoy,Gera, Meeta,Kim, Nameun,Park, Yang Ho,Kwon, Taeho,Jeong, Dong Kee Elsevier 2018 Biochemical and biophysical research communication Vol.503 No.4
<P><B>Abstract</B></P> <P>Pancreatic ductal adenocarcinoma (PDAC) is a major malignant phenotype in pancreatic cancer, which is one of the most death causes by cancer in the world. PDAC developed from pancreatic intra-epithelial neoplasms (PanINs) and poorly diagnosed at early stages. Beside of high drug resistance, metastasis is the great concern during pancreatic cancer treatment. SALL4 expression is inherent in the upregulations of endothelial mesenchymal transition (EMT) genes and therefore promoting cancer metastasis. Furthermore, some of evidences indicated reactive oxygen species (ROS) is also influent to metastasis and self-antioxidant capacity seems a gold standard for successful metastasis rate. In this study, we have found the role Spalt like protein 4 (SALL4) to PDAC proliferation, mobility and its regulation to mitochondrial ROS via FoxM1/Prx III axis. It is possible that SALL4 mainly induces endothelial-mesenchymal transition (EMT) phenotype and favors ROS loss to facilitate metastasis efficiency in PDAC cells. Therefore, SALL4 might be a promising marker for PDAC treatment and targeting SALL4 would benefit anti-proliferative and anti-metastasis therapies.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SALL4 promotes stemness traits and metastatic phenotype <I>in vitro</I>. </LI> <LI> SALL4 expression positively impacts PDAC-derived tumor growth. </LI> <LI> SALL4 regulates intracellular ROS via FoxM1/Prx III by activation of ERK1/2. </LI> </UL> </P>
Zhang, Jiao Jiao,Kang, Tae Yoon,Kwon, Taeho,Koh, Hyebin,Chandimali, Nisansala,Huynh, Do Luong,Wang, Xian Zhong,Kim, Nameun,Jeong, Dong Kee American Society for Microbiology 2019 Infection and immunity Vol.87 No.3
<P><I>Gallibacterium anatis</I> is a pathogen associated with peritonitis and salpingitis in chickens and other avian species. Novel safety prevention strategies are urgently needed because of widespread multidrug resistance and antigenic diversity.</P><P><I>Gallibacterium anatis</I> is a pathogen associated with peritonitis and salpingitis in chickens and other avian species. Novel safety prevention strategies are urgently needed because of widespread multidrug resistance and antigenic diversity. The objective of this study was to produce a specific chicken egg yolk antibody and evaluate its protective response against a <I>G. anatis</I> infection model in 4-week-old chicks. Enzyme-linked immunosorbent assays showed that hens immunized with the recombinant N terminus of <I>Gallibacterium</I> toxin A (GtxA-N) had significantly increased antibody titers against GtxA-N in serum and egg yolk IgY. Western blotting showed that IgY antibody had specificity against GtxA-N in the egg yolks of immunized hens. The growth of <I>G. anatis</I> in brain heart infusion (BHI) broth and agar was significantly inhibited by the GtxA-N-specific IgY antibody. The protective effects of the specific IgY antibody were evaluated in <I>G. anatis</I>-infected chicks after intramuscular injection (10 mg/ml). The anti-GtxA-N antibody titers in the sera of <I>G. anatis</I>-challenged chicks following an injection of specific IgY antibody were significantly higher than those of the control and the nonspecific IgY groups, but lower lesion scores for the peritoneum, liver, and duodenum were found after specific IgY antibody treatment. The results from this study suggest that the GtxA-N-specific IgY antibody could potentially improve the protective response against <I>G. anatis</I> infection in chicks.</P>
Innovative Approach of Non-Thermal Plasma Application for Improving the Growth Rate in Chickens
Zhang, Jiao Jiao,Wang, Xian Zhong,Kwon, Taeho,Huynh, Do Luong,Chandimali, Nisansala,Kim, Nameun,Kang, Tae Yoon,Ghosh, Mrinmoy,Gera, Meeta,Lee, Sang Baek,Lee, Sung Jin,Lee, Wang Shik,Kim, Seong Bong,Mo MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.8
<P>As an innovative technology in biological applications—non-thermal plasma technique—has recently been applied to living cells and tissues. However, it is unclear whether non-thermal plasma treatment can directly regulate the growth and development of livestock. In this study, we exposed four-day-incubated fertilized eggs to plasma at 11.7 kV for 2 min, which was found to be the optimal condition in respect of highest growth rate in chickens. Interestingly, plasma-treated male chickens conspicuously grew faster than females. Plasma treatment regulated the reactive oxygen species homeostasis by controlling the mitochondrial respiratory complex activity and up-regulating the antioxidant defense system. At the same time, growth metabolism was improved due to the increase of growth hormone and insulin-like growth factor 1 and their receptors expression, and the rise of thyroid hormones and adenosine triphosphate levels through the regulation of demethylation levels of growth and hormone biosynthesis-related genes in the skeletal muscles and thyroid glands. To our knowledge, this study was the first to evaluate the effects of a non-thermal plasma treatment on the growth rate of chickens. This safe strategy might be beneficial to the livestock industry.</P>
Do Huynh Luong,Neelesh Sharma,Nameun Kim,Jiao Jiao Zhang,Nisansala Chandimali,Mrinmoy Ghosh,Meeta Gera,Taeho Kwon,Dong-Kee Jeong 한국발생생물학회 2017 한국발생생물학회 학술발표대회 Vol.2017 No.8
Bovine mammalian gland has biopotential in therapeutic protein production and could be used as a model in further lactation researches. In this study, we have isolated bovine mammary gland-derived epithelial cells (BMECs) from Korean Holstein dairy cattle and themselves show differential dynamic ability in in-vitro culture. BMECs enables to form lobulo-alveolar structure, express milk production related gene. Functional studies indicated that BMECs secret exogeneous antibacterial fragment-Bovine Lactoferricin B (bLfcin-B), which is inserted in PiggyBac system, and this bioactive fragment inhibits the growths of Escherichia coli and Staphylococcus aureus. These data demonstrated that BMECs open new scope in either bioactive fragment, heading to prevent the spread of mastitis or post-mastitis damage in dairy graze and could be an ideal bioreactor for antibacterial proteins.
Yeong-Seon Won,Seung-Jae Lee,Hyung-Jin Lim,Jaehoon Bae,Seon Gyeong Bak,Nisansala Chandimali,Eun Hyun Park,Sang-Ik Park,Hirofumi Tachibana 한국식품영양과학회 2023 Journal of medicinal food Vol.26 No.10
The discovery of limocitrin in Sedum sarmentosum Bunge, a compound known for its potent antitumor activity, hassparked interest in understanding its molecular mechanisms and bioactive effects. Breast cancer, particularly triple-negative breastcancer (TNBC), presents a challenging prognosis with a higher likelihood of recurrence, metastasis, and lower survival ratescompared tomost other cancer types. This study aimed to explore the anticancer potential of limocitrin on two different human breastcancer cell lines. The results of the study revealed that limocitrin effectively reduced the viability of breast cancer cells, with IC50values of 29.33 – 0.010 and 28.70 – 0.030 lM for MDA-MB-231 and MCF-7 cells, respectively. Further investigations demonstratedthat limocitrin induced apoptotic cell death, characterized by an increase in the population of apoptotic cells and the formationof apoptotic bodies. Limocitrin induced the upregulation of apoptosis-related protein expressions such as apoptosis-inducing factor,Bax, endonuclease G, and cleaved-poly ADP-ribose polymerase, while downregulating the expression of proteins associated withcell survival, including Akt, Bcl-2, Bid,mTOR, PI3K, procaspases, and p70 S6 kinase.Notably, the response to limocitrin treatmentvaried between the two types of breast cancer cells, indicating a differential effect of limocitrin on the intracellular signalingpathways related to cell survival in breast cancer.These findings open up avenues for further research and exploration of limocitrin asa potential therapeutic agent for breast cancer treatment, especially for challenging subtypes like TNBC.