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Improved Micropropagation of Root Chicory, Cichorium intybus L. var. sativus.
Lim,Jung-Dae,Yang,Deok-Chun,Lee,Hyeon-Yong,Kim,Jong-Dai,Lee,Jin-Ha,Sung,Eun-Soo,Yu Chang-Yeon 한국자원식물학회 2004 Plant Resources Vol.7 No.1
The establishment of an efficient protocol for plant regeneration and micropropagation from leaf explant cultures of Chicory, Cichorium intybus L. var. sativus. is reported. Callus formation rate appeared 100% from explant in all growth regulators, but calli formed in the prensence of naphthaleneacetic acid (NAA) were appeared very compact and non-embryogenic state. The regenerated shoots were obtained from leaf explant cultures on solid MS medium containing different concentrations of cytokinins and auxin. The highest number of shoots (5.7) per explant and shoot growth (2.8cm) was obtained on MS medium containing 0.1 mg BAP L^-1 and 0.1 mg NAA L^-1. Indole acetic acid was the most suitable auxin for root formation among three auxins tested. 2,4-D had no effect on shoot and root formation.
Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9
Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.
Dae-Weon Lee,Il Sung Moon,강재순,Hyun Jung Choe,최재영,제연호,이시혁,Hye Rim Han,Kook-Jin Lim6,Young Ho Koh 한국응용곤충학회 2007 Journal of Asia-Pacific Entomology Vol.10 No.2
Bursaphelenchus xylophilus has been recognized as a causal pathogen of pine wilt disease (PWD). In order to identify relevant molecular biomarkers, we selected a variable region of endo-β-1,4-glucanase (β14-gcn) which was cloned from the cDNA of B. xylophilus and B. mucronatus. We then identified three novel β14-gcn isoforms: Bm-β14-gcn and Bm-β14-gcni from B. mucronatus, and Bx-β14-gcn from B. xylophilus. Bm-β14-gcn and Bx-β14-gcn were found to be identical, whereas Bm-β14-gcni was unique to B. mucronatus. These isoforms are Bursaphelenchus-specific and may be employed as molecular markers for the diagnosis of PWD. Our phylogenetic analysis showed that these β14-gcns from Bursaphelenchus species were associated most closely with fungal β14-gcns.
Park, Jinbong,Jeon, Yong-Deok,Kim, Hye-Lin,Lim, Hara,Jung, Yunu,Youn, Dong-Hyun,Jeong, Mi-Young,Kim, Hyun-Ju,Kim, Sung-Hoon,Kim, Su-Jin,Hong, Seung-Heon,Um, Jae-Young Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-
<P>Obesity has become a major health threat in developed countries. However, current medications for obesity are limited because of their adverse effects. Interest in natural products for the treatment of obesity is thus rapidly growing. Korean Medicine (KM) is characterized by the wide use of herbal formulas. However, the combination rule of herbal formulas in KM lacks experimental evidence. According to <I>Shennong's Classic of Materia Medica</I>, the earliest book of herbal medicine, <I>Veratrum nigrum</I> (VN) has antagonistic features against <I>Panax ginseng</I> (PG), and the PG-VN pair is strictly forbidden. In this study, we have shown the effects of PG, VN, and their combination on obesity in high-fat (HF) diet-induced obese mice and in 3T3-L1 cells. PG, VN, and PG-VN combination significantly reduced weight gain and the fat pad weight in HF diet-induced obese mice. They also significantly decreased lipid accumulation and the expressions of two major adipogenesis factors, PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I>, in 3T3-L1 cells. In addition, the PG-VN combination had synergistic effects compared with the mixture of extracts of PG and VN on inhibition of PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I> expressions at lower doses. These results indicate a new potential anti-obese pharmacotherapy and also provide scientific evidence supporting the usage of herbal combinations instead of mixtures in KM.</P>
Jin-Seok Kim,Sung-Hun Lim,Jae-Chul Kim,Jong-Fil Moon IEEE 2013 IEEE transactions on applied superconductivity Vol.23 No.3
<P>This paper analyzes the bus voltage sags in a power distribution system with a superconducting fault current limiter (SFCL). Among various SFCLs, the composite type with a superconducting element in parallel with a current limiting reactor (CLR) is more effective than resistor-type SFCLs because it reduces the burden on the superconducting element, ac loss, and cost. First, a composite-type SFCL is modeled using PSCAD/EMTDC. Next, a power distribution system with a reclosing protective scheme is modeled. The bus voltage sags depend on the CLR magnitude in the SFCL and the fault period in the power distribution system; the magnitude of voltage sags depends on the magnitude of the fault current, and the durations of the voltage sags depend on the fault period, which is determined by the reclosing time. Therefore, the effects on voltage sags are assessed when an SFCL with various CLR magnitudes is installed in a power distribution system, and the fault duration is changed according to the reclosing time of the protective device.</P>
The Response of Diabetic Rabbit Femoral Artery to Vasoactive Drugs
Lim, Jin-soo,Hong, Jeung-Keun,Yi, Sok Ki,Kwon, Ho,Chung, Sang Hun,Lim, Poong 가톨릭대학교 2000 Bulletin of The Catholic Research Institutes of Me Vol.28 No.-
Patients with diabetes mellitus are known to develop various vascular complications such as cardiovascular disease, thrombosis, and atherosclerosis. Ti has been suggested that these complications are associated with an alteration in the reactivity of blood vessels to neurotransmitters and hormones. The effects of vasoactive agents on the vessels in diabetes mellitus have been investigated extensively, but there is not unified agreement upon the effects due to the use of different diabetogenic agents, duration of diabetes, species of animal, and type of vessels. Moreover studies using diabetic rabbit femoral artery are lacking in the literature. This experiment was aimed to investigate the contractile responses of the femoral artery to phenylephrine and the vasodilatory effects of verapamil and papaverine on the precontracted femoral artery in the control(n=20) and the diabetic rabbit(n=16). Twenty diabetic rabbits were made by the administration of alloxan(100mg/kg) intravenously and sacrified 8 weeks later. Femoral arterial rings 3mm in length were taken and mounted on the force-displacement transducer for the measurements of isometric tension. All experiments were done in the aerated(95% O₂ with 5% CO₂)biological chamber filled with Kreb's solution and the initial tension of 1.5 g was applied to the rings. After 1hour of equilibrium of the rings, contractile responses were obtained by increasing the concentration of the phenylephrine in the chamber(10□ M~10□ M). Vasodilatory responses were also obtained by cumulatively increasing the total concentration of the verapamil(10□ M~10□ M) and papaverine(10□ M ~ 10□) after precontraction with phenylephrine(10□ M in control, 10□ M in diabetic). The results are as follows. 1. The contractile responses of the femoral arterial rings to phenylephrine(10□ ~10□ M) were decreased significantly in the diabetic rabbits compared with the control rabbits(P<0.01). 2. Significant impairment of verapamil- and papaverine- induced relaxation was observed in the precontracted femoral arterial rings of the diabetic rabbits compared with that of the control rabbits(verapamil ; 10□ M ~ 10□ M : P<0.01, papaverine ; 10□ & 10□ M : P<0.05, 10□ M : P<0.01) By this study, we found that the contractile responses of the femoral arterial rings to phenylephrine were decreased in the rabbits, and the vasodilator effects of verapamil and papaverine on th precontracted femoral arterial rings were also lowered in diabetic rabbits compared with the control rabbits. (The Journal of the Korean Society of Reconstructive Hand Surgery 4(1): 7-15, 1999)