Vulnerability of DNA hybridization in soils is due to Mg²⁺ ion induced DNA aggregation

Wang, Xiaofang,Kweon, Hyojin,Lee, Seokho,Shin, Hyejin,Chua, Beelee,Liles, Mark R.,Lee, Ming-kuo,Son, Ahjeong Elsevier 2018 Soil biology & biochemistry Vol.125 No.-

<P><B>Abstract</B></P> <P>The NanoGene assay is an inhibitor-resistant gene quantification assay based on magnetic bead and quantum dot nanoparticles. It employs a set of probe and signaling probe DNAs to capture target DNA via hybridization. Using simple DNA preparation that bypasses conventional DNA extraction, it was able to detect and quantify specific bacterial genes in environmental sample. In this study, the vulnerability of the NanoGene assay to the presence of various environmental factors was investigated. A total of 43 soil samples were inoculated with 10<SUP>9</SUP> CFU/mL of <I>Pseudomonas putida</I> prior to DNA isolation without purification. Subsequently, the NanoGene assay was performed for quantitative detection of <I>P. putida</I> with respect to 12 soil properties including pH, moisture, humic acids, organic matter, sand, silt, clay, cation exchange capability, sodium, potassium, magnesium, and calcium. Using multiple linear regression, the NanoGene assay was found to be particularly vulnerable to the presence of Mg<SUP>2+</SUP>, which was selected as a major variable (<I>P</I> = 0.001). The vulnerability of the NanoGene assay to Mg<SUP>2+</SUP> was further explored by atomic force microscopy, which indicated significant Mg<SUP>2+</SUP>-mediated DNA aggregation. The inhibition of the NanoGene assay from some soil samples as a consequence of DNA aggregation could therefore be prevented by the use of Mg<SUP>2+</SUP> chelators such as EDTA, enabling application of this method across diverse soil types.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The NanoGene assay was able to detect and quantify specific bacterial genes in environmental soils with minimum inhibition. </LI> <LI> The effect of various soil properties to the bacterial detection by NanoGene assay was investigated over diverse soil types. </LI> <LI> Multiple linear regression indicated the major soil property that affects the NanoGene assay is Mg<SUP>2+</SUP> ion in soils. </LI> <LI> Further investigation by atomic force microscopy indicated it was due to the Mg<SUP>2+</SUP>-mediated DNA aggregation. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Electroless Route to Prepare Cu Film Composed of Cu Nanosheets as a High Performance Catalyst for the Reduction of p-Nitrophenol

Liling Li,Jinyun Liao,Haitao Wang,Bruno G. Pollet,Hao Li 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.12

A Secure and Efficient Multi-Factor Mutual Certificateless Authentication with Key Agreement Protocol for Mobile Client-Server Environment on ECC without the third-party

Liling Cao,Wancheng Ge 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.10

Authentication with key agreement (AKA) protocols are implemented to provide identity authentication and session keys for communication entities. In order to reduce the heavy trust reliance on key generator center (KGC) in identity based AKA protocols, a certificateless based AKA (CLAKA) protocol for client-server environment without the third-party (i.e., KGC) is introduced in this paper. The proposed protocol is constructed based on elliptic curve cryptosystem (ECC) and multi-factor protections (such as password, biometrics, and smart card). Moreover, security proof based on BAN-logic is carried out and shows that our protocol can provide mutual authentication, user anonymity, dynamic identity and perfect forward security, and resist to user impersonation attack, server spoofing attack and privileged insider attack. Meanwhile, security and efficiency analysis shows that our proposed protocol outperforms the previous related ones.

Human Rights and Police’s Role in China’s Criminal Investigation

Liling Yue 아시아경찰학회 2007 아시아경찰학회 국제학술회의 Vol.2007 No.-

Analysis of Certificateless Signcryption Schemes and Construction of a Secure and Efficient Pairing-free one based on ECC

( Liling Cao ),( Andwancheng Ge ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.9

Signcryption is a cryptographic primitive that provides authentication (signing) and confidentiality (encrypting) simultaneously at a lower computational cost and communication overhead. With the proposition of certificateless public key cryptography (CLPKC), certificateless signcryption (CLSC) scheme has gradually become a research hotspot and attracted extensive attentions. However, many of previous CLSC schemes are constructed based on time-consuming pairing operation, which is impractical for mobile devices with limited computation ability and battery capacity. Although researchers have proposed pairing-free CLSC schemes to solve the issue of efficiency, many of them are in fact still insecure. Therefore, the challenging problem is to keep the balance between efficiency and security in CLSC schemes. In this paper, several existing CLSC schemes are cryptanalyzed and a new CLSC scheme without pairing based on elliptic curve cryptosystem (ECC) is presented. The proposed CLSC scheme is provably secure against indistinguishability under adaptive chosen-ciphertext attack (IND-CCA2) and existential unforgeability under adaptive chosen-message attack (EUF-CMA) resting on Gap Diffie-Hellman (GDH) assumption and discrete logarithm problem in the random oracle model. Furthermore, the proposed scheme resists the ephemeral secret leakage (ESL) attack, public key replacement (PKR) attack, malicious but passive KGC (MPK) attack, and presents efficient computational overhead compared with the existing related CLSC schemes.

A New Method Improving Image Matching Accuracy Based On Cumulative Histogram

Zhao Liling,Zheng Yuhui,Sun Quansen,Xia Deshen 보안공학연구지원센터(IJSIP) 2013 International Journal of Signal Processing, Image Vol.6 No.2

중국 패션잡지「상하이스타일(上海服飾)」에 나타난 패션스타일

황려령 ( Liling Huang ),하지수 ( Ji Soo Ha ) 한국의류산업학회 2016 한국의류산업학회지 Vol.18 No.2

Research on changes of Chinese women’s fashion styles was conducted through analysis of fashion images and articles in Chinese fashion magazines since 1980s. Through the fashion magazine research centered on Shanghai, the most developed commercial city having a great influence on the entire fashion industry in China, it examined Chinese fashion styles by period so identified a developmental process of Chinese fashion styles. Based on general understanding of Chinese fashion magazine industry, case study was conducted on 「Shanghai Style(上海服飾)」. After analyzing 1100 fashion images by period derived from 245 volumes from 1985 to 2013, this study compared fashion styles in 4 periods and analyzed the developments of Chinese fashion styles. Findings from the case analysis based on the classification of fashion styles are as follows. In the early editions, images to give a visual stimulus and express styles more intuitively were frequently used with reported articles. As for the general change of the style in 「Shanghai Style(上海服飾)」, expression was done centered on casual wear with gradual disappearance of formal wear. There was a tendency entire atmosphere of the style became romantic. Through a comparative analysis between Chinese fashion styles having such developmental characteristic and the global fashion trends, this study identified special characteristics of Chinese fashion style changes. As for common characteristics, firstly, both have a tendency to change into the coexistence of various styles rather than popularity of certain style. Secondly, style was expressed gradually centered on casual wear.

Fault-Tolerant Control Based on Modified eXogenous Kalman Filter for PMSM

Ma Liling,Wang Fuxiang,Shen Wei,Wang Junzheng 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.2

Aiming at the encoder fault of the three-phase permanent magnet synchronous motor control system, a modified eXogenous Kalman Filter (MXKF) is proposed. When there are errors in the initial state of the estimator, MXKF performs well while extended Kalman Filter leads to an unstable state of the system. For the system under the Three-phase Four-switch drive, MXKF is shown to have better fault-tolerant stability. In addition, to avoid the unexpected equilibrium points determined by the q-axis voltage equation, proper compensation is made in the filter. Finally, simulations have been done to verify the effectiveness of the proposed method in the following two cases: start-up with initial error and topology change of the drive. The results show the satisfying superiority of MXKF.

ノンネイティブ日本語教師研修の史的変遷とその課題 ― 国際交流基金による教師研修の目的に注目して ―

成利楽 ( Cheng Lile ) 한국일어교육학회 2022 일본어교육연구 Vol.- No.61

本研究は,日本国外で日本語教育を担っている主力であるノンネイティブ日本語教師を対象とする教師研修の史的変遷とその課題を探ることを目的とする。そのため,主な担当機関である国際交流基金が実施した研修を報告した論考を分析材料とし,その研修目的に着眼しながら,これまでのノンネイティブ日本語教師研修の史的変遷を辿った。KH Coderで共起ネットワークを構築したところ,全体的に日本語力,文化関連知識·能力と教授能力の開発が中心的な目的であったことがわかった。また,年代ごとのネットワークを確認した結果,1990年代は日本語運用能力の向上,日本文化,異文化理解の促進や日本語教授法の体験,2000年代は協働と内省による教師の意識開発,2010年代は省察的実践力の育成,2020年代は教師の深い学びの実現といった研修目的が中心であった。この結果を母語話者日本語教師教育の言説と比較することで,非母語話者教師を対象とする研修が日本国内における母語話者日本語教師研究からの影響を受けた可能性があるという課題の発見に至った。それに対して,今後非母語話者教師の養成体制や成長課題を考慮した上での教師研修の必要性について提言した。 The purpose of present study is to explore the historical trends and issues in teacher training for non-native Japanese language teachers(NNT) outside of Japan. As for the method, research of teacher training about NNTs from the 1990s were collected and analyzed from the perspective of training purposes. A keywords network for each age group was constructed by KH Coder. As a result, Japanese language proficiency, culture-related knowledge and skills, and teaching skills were the central objectives of these training sessions. The main objectives of the training 1) in the 1990s were to improve Japanese language skills, promote understanding of cultures, and experience teaching methods; 2) in the 2000s, to develop teachers' awareness through collaboration and reflection; 3) in the 2010s, to develop reflective practice skills; and 4) in the 2020s, to realize deep learning for teachers. A comparison of these results with the discourse about Japanese teacher education led to the inference that training activities for NNTs may have been influenced by the discourse of native speaker teacher education in Japan. In response, we propose the need for future teacher training that considers training system of NNT and their professional development challenges.

Prefabrication of Axially Vascularized Bone by Combining β-Tricalciumphosphate, Arteriovenous Loop, and Cell Sheet Technique

Dongyang Ma,Liling Ren,Zhen Cao,Jianxue Li,Jian Cao,Wenyan Tian,Hong Yao 한국조직공학과 재생의학회 2016 조직공학과 재생의학 Vol.13 No.5

The repair of bone defects poses a great challenge for reconstructive surgeons. Although the development of tissue engineering has exhibited promise in replacing damaged bone, the fabrication of large constructs with functional blood vessels remains an obstacle. From the orthopedic surgeon’s point of view, the generation of axially vascularized bone, which can anastomose with the recipient vessel, might be a solution to this medical problem. In this study, we aimed to prefabricate an axially vascularized bone by combining a β-TCP scaffold, arteriovenous loop (AVL), and cell sheet in a bioreactor in vivo. Twelve rabbits were randomly allocated into two groups: the experimental group (presence of AVL) and the control group (absence of AVL). The constructs were explanted at 8 weeks postoperatively. The histomorphometric results showed 42.8±5.9% of the bone area in the AVL group and 26.6±3.5% in the control group. Similarly, vessel analysis revealed the average vessel density in the AVL group (12.5±3.3) was significantly more than that in the control group (6.1±1.5, p<0.05). Our research indicated that the combination of a β-TCP scaffold, AVL and cell sheet might engineer vascularized bone. This prefabrication strategy might facilitate clinical translation of bone tissue engineering in reconstructing large bone defects.