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      • Contrastive Analysis on Network Performance of Information System

        Lifeng Jiang,Fengming Zhang,Rennong Yang,Kun Xu 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.7

        In order to explore how to design an information system with ideal network property under the background of information era, contrastive analysis is conducted in this paper on the performances of “tree” network and fully connected network. The mathematical models of “tree” information system and fully connected network are established, of which the network performance indicators are calculated by complex network theory and analyzed by comparison. As only partial indicators of both networks are favorable, the plan is proposed that information system is established through optimization based on both of the two network structures. The new algorithm to build information system network model proposed in this paper and the plan that information system is established are of significant reference value for relevant fields.

      • KCI등재

        Study of the departure of pine wood nematode, Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) from Monochamus alternatus (Coleoptera: Cerambycidae)

        Wang Yang,Chen Fengmao,Wang Lichao,Zhou Lifeng,Song Juan 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4

        In order to study the causes of pine wood nematode (PWN) departure from Monochamus alternatus, the effects of the feeding behavior of M. alternatus on the start date of the departure of PWN were studied. The start date of the departure of PWN carried by the directly fed M. alternatus was 5–13 d after beetle emergence, mainly concentrated within 6–10 d, with a mean ( ± SD) of 8.02 ± 1.96 d. The start date of the departure of PWN carried by the M. alternatus fed after starvation was 5–14 d after beetle emergence, mainly concentrated within 6–9 d, with a mean of 7.76 ± 2.28 d. The results show that there was no significant difference in the start departure date of PWN between the two treatments. This shows that the feeding behavior of M. alternatus is not the trigger for PWN departure. At the same time, it was found that the motility of the PWN carried by M. alternatus at 8 d after emergence was significantly greater than that of the PWN carried by the newly emerged M. alternatus. And the PWN carried by M. alternatus at 8 d after emergence was extracted more easily than the PWN carried by newly emerged beetles. These results show that greater motility was associated with easier departure of PWN from M. alternatus. In addition, transcriptome sequencing found that the level of oxidative phosphorylation metabolism of PWN carried by beetles at 8 d after emergence was significantly higher than that in the PWN carried by newly emerged beetle. High oxidative phosphorylation was associated with increased energy production and motility by the PWN and were the internal cause of the start of nematode departure.

      • KCI등재

        Quantitative Detection of Corroded Reinforced Concrete of Different Sizes Based on SMFL

        Mao Yang,Jianting Zhou,Qingyuan Zhao,Qianwen Xia,Hong Zhang,Lifeng Wang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.1

        In order to solve the problem of quantitative detection of corroded reinforced concrete of different sizes, the quantitative detection experiment based on spontaneous magnetic flux leakage (SMFL) was carried out in batches. Electrochemical corrosion of 27 reinforced concrete specimens was carried out, and the SMFL signals of reinforcement were obtained by magnetic detection equipment. Four-dimensional magnetic indicators M1-M4 that can characterize the corrosion degree of the specimens were defined. The influence of different sizes on the magnetic indicators were analyzed. It is concluded that the thicknesses of the concrete covers affect the magnetic indicators by affecting the lift-off heights z. The influence of diameter of the rebars on the quantitative detection can be eliminated by describing the corrosion degree with the average cross-section loss rate α. The influence of length of the rebars on quantitative detection is not clear yet. Finally, Support Vector Machine (SVM) was introduced to establish a classification model of corrosion classes and magnetic indicators. Using the model to predict the corrosion classes of the specimens can achieve a high classification accuracy. The research provides a new method for the quantitative detection of steel corrosion.

      • KCI등재

        Engineering of a Bacillus subtilis Strain Deficient in Cellulase: Application in Degumming of Ramie

        Qi Yang,Shengwen Duan,Lifeng Cheng,Xiangyuan Feng,Ke Zheng,Chunliang Xie,Zhiyuan Liu,Yuande Peng 한국섬유공학회 2019 Fibers and polymers Vol.20 No.1

        Degumming is the most important step before ramie fibers are used in textile industry. Conventional chemical degumming technology with alkaline treatment at high temperature causes critical problems in environment. In addition to multiple degumming enzymes (e.g. pectinases and xylanases), Bacillus subtilis strain 168 can also produce cellulase which brings irreversible damage to ramie fibers. In this study, a strain deficient in cellulase was constructed by insertional inactivation of gene eglS to make it suitable for microbial degumming. Results showed that the cellulase activity was not detected in strain ΔeglS, after 25 h of incubation with strain ΔeglS, the weight loss of the ramie fibers was 20.20 %. Though the residual gum content of ramie fibers obtained in microbial degumming process was 19.67 %, microbial intervention followed by diluted alkaline solution treatment showed lower residual gum content (2.73 %) than chemical degumming (2.96 %). The single fiber breaking strength value of ramie fibers from bio-chemical degumming process reached 27.19 cN, which was the highest of three samples. Moreover, bio-chemical degumming process resulted in a 75 % decrease of alkali dosage as compared with chemical degumming process. This study provides a suggestion to genetically modify wild-type strain in order to protect ramie fibers from cellulase damage, and indicates a sustainable alternative for the traditional chemical degumming in the textile industry.

      • KCI등재

        Alkaline responsive self-healing nanocontainer composite reverse osmosis membrane by layer self-assembly: Enhanced permeable and chlorine resistance properties

        Xiao Xie,Qian Yang,Qiong Sun,Na Song,Liyan Yu,Lifeng Dong 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.113 No.-

        Low water flux and poor chlorine resistance have become barriers to the applications of polyamidereverse osmosis (RO) membranes. Here, we design and develop a novel RO membrane with high permeabilityand self-healing chlorine resistance capability by doping alkaline-responsive polymer nanocontainersinto the polyamide layer. The nanocontainer is prepared through chemical adsorption andelectrostatic self-assembly with titanium dioxide (TiO2) as the core, calcium alginate (CA) and chitosan(CS) as the repair materials, and polyaspartic acid (PASP) as the responsive shell. In addition to increasingwater transport through the channels, the PASP shell of the nanocontainer reacts with alkali during conventionalalkaline cleaning and thereby the CA and CS are released to precisely repair the chlorinatedpolyamide and restore the NaCl rejection of the RO membrane. Upon release of the nanocontainer,TiO2 is also exposed to make the membrane antibacterial. The nanocontainer doping significantlyenhances surface roughness of the RO membrane, and the water permeability of the thin-film nanocompositemembrane doped with 0.005 wt% nanocontainers is increased by 43.71% to 5.03 L/m2 h bar comparedwith the blank membrane, while performing an excellent NaCl rejection of 98.02% and maintaining95.95% after 8000 ppm h active chlorine treatment and alkaline cleaning process.

      • KCI등재

        miR156b from Soybean CMS Line Modulates Floral Organ Development

        Xianlong Ding,Hui Ruan,Lifeng Yu,Qiang Li,Qijian Song,Shouping Yang,Junyi Gai 한국식물학회 2020 Journal of Plant Biology Vol.63 No.2

        The miR156 and plant specifc transcription factor SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) are known for their function regulating plant growth and development. In this study, we identifed 20 GmSPLs which are targeted by gma-miR156b via plant small RNA target and degradome analysis. And we found high transcript levels of gma-miR156b and its targeted GmSPLs in the fower of soybean cytoplasmic male sterility (CMS) line and its maintainer. The gma-miR156b direct cleavage of GmSPL2b and GmSPL9b, and have opposite expression levels during early fower buds development. We observed a high expression level of GUS protein in the anthers of the line with pgma-MIR156b::GUS reporter. Over-expression of the gma-miR156b precursor in Arabidopsis inhibited foral organ development, including reduced anther size and the amount of pollen grains per anther etc. Like miR156-targeted SPL genes, non-targeted GmSPL8s were also down-regulated in early fower bud development of soybean CMS line compared with its maintainer line, which might act in concert with miR156-targeted SPL genes to participate in the foral organ development. Quantitative real time PCR (qRT-PCR) suggested that miR156/SPL modulates foral organ development by regulating the expression of LATERAL ORGAN BOUNDARIES DOMAIN22 (LBD22), LBD36, AGAMOUS-LIKE30 (AGL30) and AGL104. Our fndings will facilitate understanding of the biological functions of miR156/SPL in foral organ development of soybean CMS.

      • KCI등재

        Oxygen vacancies assisted LaFeO3 derived from metal organic frameworks endows a practical HCHO sensor with excellent sensing characteristics

        Lulu Guo,Shushu Zhao,Guimao Yang,Lifeng Gao,Yanhong Wu,Xuguang Zhang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.126 No.-

        Perovskite oxide semiconductors have attracted tremendous interest in gas sensing due to their promisingproperties of tunable active sites, excellent catalytic ability and good structural stability. Nevertheless, the rapid synthesis of perovskite oxides and controlled regulation of their surface oxygenvacancies remains a great challenge. Herein, we report a novel metal–organic frameworks (MOFs) selftemplatestrategy for the rapid and large-scale preparation of LaFeO3 nanoparticles (MLaFeO3) withabundant oxygen vacancies. Benefit from the introduction of oxygen vacancies, the resultantMLaFeO3 gas sensor exhibit excellent formaldehyde (HCHO) sensing performance at a low operatingtemperature of 160 C, including high sensitivity (Rg/Ra = 8.9 @ 100 ppm), fast response/recovery rate(53 s/32 s), low detection limit (1 ppm) and excellent selectivity. Comprehensive density functional theory(DFT) calculation and spectral characterizations reveal that oxygen vacancies play a vital role in promotingthe adsorption and activation of O2 and HCHO molecules, and accelerate the chemical reaction onthe sensing materials surface. Most importantly, it proves the promising application of MLaFeO3 sensorin food safety assessment. This work not only provides a simple strategy for constructing oxygen vacanciesenriched LaFeO3, but also demonstrates the application potential of LaFeO3-based gas sensors in thefield of formaldehyde detection.

      • Super-Twisting Sliding Mode Observer Based Speed Sensorless Torque Control for PMSG Used in Wind Turbines

        Leilei Guo,Xing Zhang,Shuying Yang,Zhen Xie,Lifeng Qi,Lingxiang Wang 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

        A speed sensorless torque control method for permanent magnet synchronous generator (PMSG) based on super-twisting sliding mode observer (STSMO) is proposed in this paper. Active back electromotive force (BEMF) can be obtained based on the STSMO. With the observed active BEMF, the rotor position angle and the speed are estimated by a phase-locked loop. Besides, a stator flux and electromagnetic torque (EMT) observation method is proposed based on the observed active BEMF. Compared to the traditional method, the robustness of the proposed one to the parameters of PMSG is improved. Then a speed sensorless EMT closed-loop control system is established. The simulation and experimental results verify the effectiveness of the proposed algorithm.

      • KCI등재

        High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration

        Sun Di,Mou Shan,Chen Lifeng,Yang Jie,Wang Rongrong,Zhong Aimei,Wang Wei,Tong Jing,Wang Zhenxing,Sun Jiaming 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

        Background: Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have been found to have a great potential for soft tissue repair due to various biological functions, including pro-angiogenesis and low immunogenicity. However, the low yield and heterogeneity of MSC-EVs limited their clinical transformation. This study was designed to develop a novel adipose-derived stem cell engineered nanovesicles (ADSC-NVs) with high production and explore its pro-angiogenetic effect and application in adipose tissue regeneration. Methods: Adipose-derived stem cell-derived extracellular vesicles (ADSC-EVs) were isolated from an EVs-free culture medium for human ADSCs (hADSCs). ADSC-NVs were prepared by sequentially extruding ADSCs followed by iodixanol density gradient ultracentrifugation and were compared with ADSC-EVs in morphology, size distribution, protein contents and yield. The pro-angiogenetic effect of ADSC-NVs in different doses (0, 5, 20 and 80 μg/mL) in vitro was determined using transwell assay, tube formation assay, western blot and qRT-PCR. In vivo, BALB/c nude mice were administered injection of a mixture of fat granules and different dose of ADSC-NVs and grafts were harvested at 12 weeks post-transplantation for further analysis. By analyzing the weight and volume of grafts and histological evaluation, we investigated the effect of ADSC-NVs in vessel formation and adipose tissue regeneration. Results: Our results showed yield of purified ADSC-NVs was approximately 20 times more than that of ADSC-EVs secreted by the same number of ADSCs. In vitro, both ADSC-NVs and ADSC-EVs exhibited a dose-dependent proangiogenetic effect, despite their distinct miRNA profiles. These effects of ADSC-NVs may be mediated by enriched miR-21-5p via PTEN inhibition and PI3K/p-Akt signaling activation. Furthermore, after a mixed injection of ADSC-NVs, vessel formation and adipose regeneration were observed in vivo in fat implants. Conclusions: Our study developed a potent alternative of ADSC-EVs. ADSC-NVs have a high pro-angiogenesis potential and can be used as cell-free therapeutic biomaterials in soft tissue regeneration.

      • KCI등재

        Removal of Uranium from Uranium Plant Wastewater Using Zero-Valent Iron in an Ultrasonic Field

        Jing Li,Libo Zhang,Jinhui Peng,Jinming Hu,Lifeng Yang,Aiyuan Ma,Hongying Xia,Wenqian Guo,Xia Yu 한국원자력학회 2016 Nuclear Engineering and Technology Vol.48 No.3

        Uranium removal from uranium plant wastewater using zero-valent iron in an ultrasonicfield was investigated. Batch experiments designed by the response surface methodology(RSM) were conducted to study the effects of pH, ultrasonic reaction time, and dosage ofzero-valent iron on uranium removal efficiency. From the experimental data obtained inthis work, it was found that the ultrasonic method employing zero-valent iron powdereffectively removes uranium from uranium plant wastewater with a uranium concentrationof 2,772.23 mg/L. The pH ranges widely from 3 to 7 in the ultrasonic field, and theprediction model obtained by the RSM has good agreement with the experimental results.

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