Growth of Ni/Co-Catalyzed Crystalline CNx Thin Films by Nitrogen-Plasma-Assisted Pulsed Laser Deposition

Li Li,Ning Xu,Aimin Wu,Hao Ling,Jian Sun,Xiaokang Shen 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.46 No.1

Nanocrystalline carbon nitride (CNx) thin ¯lms have been synthesized by nitrogen-plasma-assisted pulsed-laser deposition. Before depositing carbon nitride ¯lm, Ni/Co was deposited on the silicon (100) substrate acting as catalyst. The properties of the deposited ¯lms were studied by several diagnostic techniques. X-ray photoelectron spectroscopy was used for detecting the compo-sition ratio N/C in the ¯lm. From studying Raman spectra under dierent conditions we can ¯nd how glow discharge pressure aects the carbon nitride ¯lm properties. Two stages in this process and nanocrystallites of tens of nanometers embedded in the as-synthesized thin ¯lm can be seen by transmission-electron microscopy. The FTIR spectrum suggests an abundance of C-N bonds and paucity of C´N bonds, which is a favorable chance for the formation of carbon nitride crystallites. The crystalline nature of the ¯lm is detected by X-ray diraction.

Preparation and Characterization of Anti-GP73 Monoclonal Antibodies and Development of Double-antibody Sandwich ELISA

Li, Qi-Wen,Chen, Hong-Bing,Li, Zhi-Yang,Shen, Peng,Qu, Li-Li,Gong, Lai-Ling,Xu, Hong-Pan,Pang, Lu,Si, Jin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5

Background: Serum Golgi protein 73 (GP73) as a novel and potential marker for diagnosing hepatocellular carcinoma (HCC) have been found to be elevated in HCC patients and associated with clinical variables representing tumor growth and invasiveness. The aim of this study was to prepare a pair of monoclonal antibodys (mAbs) against GP73 and develop a newly designed double-antibody sandwich enzyme-linked immunosorbent assay (s-ELISA), which would be used in the detection of serum GP73 (sGP73) as well as in the diagnosis of HCC. Materials and Methods: Produced by prokaryotic expression, the purified recombinant GP73 (rGP73), produced by prokaryotic expression, was used to immunize the Balb/c mice. Two hybridoma cell lines against GP73 were obtained by fusing mouse Sp2/0 myeloma cells with spleen cells from the immunized mice. The titers of anti-GP73 mAb reached 1:243,000. Western blotting analysis and Immunohistochemistry staining revealed that anti-GP73 mAb could recognize GP73 protein. The double-antibody s-ELISA was successfully established and validated by 119 HCC and 103 normal serum samples. Results: showed that the detection limit of this method could reach 1.56 ng/ml, and sGP73 levels in HCC group (mean=190.6 ng/ml) were much higher than those of in healthy controls (mean=70.92 ng/ml). Conclusions: Results of our study not only showed that sGP73 levels of HCC patients were significantly higher than those of healthy controls, but also indicated that the laboratory homemade anti-GP73 mAbs could be the optimal tool used in evaluating sGP73 levels, which would provide a solid foundation for subsequent clinical applications.

Analysis of Cancer Incidence in Zhejiang Cancer Registry in China during 2000 to 2009

Du, Ling-Bin,Li, Hui-Zhang,Wang, Xiang-Hui,Zhu, Chen,Liu, Qing-Min,Li, Qi-Long,Li, Xue-Qin,Shen, Yong-Zhou,Zhang, Xin-Pei,Ying, Jiang-Wei,Yu, Chuan-Ding,Mao, Wei-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Objective: The Zhejiang Provincial Cancer Prevention and Control Office collected cancer registration data during 2000 to 2009 from 6 cancer registries in Zhejiang province of China in order to analyze the cancer incidence. Methods: Descriptive analysis included cancer incidence stratified by sex, age and cancer site group. The proportions and cumulative rates of 10 common cancers in different groups were also calculated. Chinese population census in 1982 and Segi's population were used for calculating age-standardized incidence rates. The log-linear model was used for fitting to calculate the incidence trends. Results: The 6 cancer registries in Zhejiang province in China covered a total of 60,087,888 person-years during 2000 to 2009 (males 30,445,904, females 29,641,984). The total number of new cancer cases were 163,104 (males 92,982, females 70,122). The morphology verified cases accounted for 69.7%, and the new cases verified only by information from death certification accounted for 1.23%. The crude incidence rate in Zhejiang cancer registration areas was $271.5/10^5$ during 2000 to 2009 (male $305.41/10^5$, female $236.58/10^5$), age-standardized incidence rates by Chinese standard population (ASIRC) and by world standard population (ASIRW) were $147.1/10^5$ and $188.2/10^5$, the cumulative incidence rate (aged from 0 to 74) being 21.7%. The crude incidence rate was $209.6/10^5$ in 2000, and it increased to $320.20/10^5$ in 2009 (52.8%), with an annual percent change (APC) of 4.51% (95% confidence interval, 3.25%-5.79%). Age-specific incidence rate of 80-84 age group was achieved at the highest point of the incidence curve. Overall with different age groups, the cancer incidences differed, the incidence of liver cancer being highest in 15-44 age group in males; the incidence of breast cancer was the highest in 15-64 age group in females; the incidences of lung cancer were the highest in both males and females over the age of 65 years. Conclusions: Lung cancer, digestive system malignancies and breast cancer are the most common cancers in Zhejiang province in China requiring an especial focus. The incidences of thyroid cancer, prostate cancer, cervical cancer and lymphoma have increased rapidly. Prevention and control measures should be implemented for these cancers.

Pigment Distribution in Fresh Shoot Tissues of Four Colored-leaf Prunus Taxa

Ya Meng Li,Xiao Ling Qu,Xiao Lei Li,Ling Guo,Xiang Shen 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Berberine Inhibits HEp-2 Cell Invasion Induced by Chlamydophila pneumoniae Infection

Li Jun Zhang,Wei Quan,Bei Bei Wang,Bing Ling Shen,Teng Teng Zhang,Yi Kang 한국미생물학회 2011 The journal of microbiology Vol.49 No.5

This study investigated the inhibitory effects of berberine on Chlamydophila (Chlamydia) pneumoniae infection-induced HEp-2 cell invasion and explored the possible mechanisms involved in this process. C. pneumoniae infection resulted in a significant increase in HEp-2 cell invasion when compared with the control cells (P<0.01) in a Matrigel invasion assay. This enhanced cell invasion was strongly suppressed by berberine (50 μM) (P<0.01). In a cell adhesion assay, the infection-induced HEp-2 cell adhesion to Matrigel was also significantly inhibited by berberine (P<0.01). C. pneumoniae infection was found to promote HEp-2 cell migration remarkably (P<0.01), which was markedly suppressed by berberine (P<0.01) in the cell migration assays. There were no statistically significant differences in the expression of matrix metalloproteinase-1 (MMP-1) and MMP-9 in the infected cells and berberine did not change the expression of MMP-1 and MMP-9. These data suggest that berberine inhibits C. pneumoniae infection-induced HEp-2 cell invasion through suppressing HEp-2 cell adhesion and migration, but not through changing the expression of MMP-1 and MMP-9.

Identification of a Novel Streptomyces chattanoogensis L10 and Enhancing Its Natamycin Production by Overexpressing Positive Regulator ScnRII

Yi-Ling Du,Shi-Fei Chen,Liang-Ying Cheng,Xue-Ling Shen,Yuan Tian,Yong-Quan Li 한국미생물학회 2009 The journal of microbiology Vol.47 No.4

A novel Streptomyces strain, L10, which is capable of producing natamycin, was isolated from a soil sample collected from Zhejiang province, China. On the basis of phylogenetic analysis of rpoB gene and 16S rDNA sequences, as well as phenotypic comparison, strain L10 (CGMCC 2644) is proposed to be a previously uncharacterized strain of S. chattanoogensis. By screening a cosmid library of strain L10 and primer walking, a partial sequence of scnRI and the entire sequence of scnRII were obtained, which are orthologues to the pathway-specific positive regulator genes of natamycin biosynthesis in S. natalensis. The engineered S. chattanoogensis D1, generated by inserting an additional copy of scnRII into the chromosome of strain L10, increased its natamycin production by 3.3 fold in YSG medium and 4.6 fold in YEME medium without sucrose.

Combined Assessment of Serum Alpha-Synuclein and Rab35 is a Better Biomarker for Parkinson’s Disease

Hung-Li Wang,Chin-Song Lu,Tu-Hsueh Yeh,Yu-Ming Shen,Yi-Hsin Weng,Ying-Zu Huang,Rou-Shayn Chen,Yu-Chuan Liu,Yi-Chuan Cheng,Hsiu-Chen Chang,Ying-Ling Chen,Yu-Jie Chen,Yan-Wei Lin,Chia Chen Hsu,Huang-Li 대한신경과학회 2019 Journal of Clinical Neurology Vol.15 No.4

Background and Purpose It is essential to develop a reliable predictive serum biomarker for Parkinson’s disease (PD). Te accumulation of alpha-synuclein (αSyn) and up-regulated expression of Rab35 participate in the etiology of PD. Te purpose of this investigation was to determine whether the combined assessment of serum αSyn and Rab35 is a useful predictive biomarker for PD. Methods Serum levels of αSyn or Rab35 were determined in serum samples from 59 sporadic PD patients, 19 progressive supranuclear palsy (PSP) patients, 20 multiple system atrophy (MSA) patients, and 60 normal controls (NC). Receiver operating characteristics (ROC) curves were calculated to determine the diagnostic accuracy of αSyn or/and Rab35 in discriminating PD patients from NC or atypical parkinsonian patients. Results The levels of αSyn and Rab35 were increased in PD patients. The serum level of Rab35 was positively correlated with that of αSyn in PD patients. Compared to analyzing αSyn or Rab35 alone, the combined analysis of αSyn and Rab35 produced a larger area under the ROC curve and performed better in discriminating PD patients from NC, MSA patients, or PSP patients. When age was dichotomized at 55, 60, 65, or 70 years, the combined assessment of αSyn and Rab35 for classifying PD was better in the group below the cutof age than in the group above the cutof age. Conclusions Combined assessment of serum αSyn and Rab35 is a better biomarker for discriminating PD patients from NC or atypical parkinsonian patients, and is a useful predictive biomarker for younger sporadic PD patients.

Characterizations of Ru/ZnO catalysts with different Ru contents for selective hydrogenation of crotonaldehyde

Bo Li,Meng-Fei Luo,Geng-Shen Hu,Ling-Yun Jin,Xiao Hong,Ji-Qing Lu 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.1

Ru catalysts supported on ZnO with different Ru contents were prepared by an impregnation method and were applied to the vapor-phase selective hydrogenation of crotonaldehyde. The catalysts were characterized by X-ray powder diffraction (XRD), NH3 temperature-programmed desorption (NH3-TPD),transmission electron microscopy (TEM) and temperature-programmed oxidation (TPO). It was found that with increasing Ru contents in the Ru/ZnO catalysts, the activity (TOF), surface acidity amount and deactivation rate increased and the selectivity to crotyl alcohol increased first and then decreased. The 3Ru/ZnO catalyst showed the highest selectivity to crotyl alcohol (up to 88.0%) for the hydrogenation of crotonaldehyde. The initial TOF values of the catalysts depended on the strength of surface acidity and the Ru particle sizes. The more Lewis acid sites made catalysts deactivate more easily. It was assumed that the deactivation was due to the formation of organic compounds deposition and poison effect of CO strongly adsorbed on the Ru atoms.

Polymorphisms of TERT and CLPTM1L and the Risk of Hepatocellular Carcinoma in Chinese Males

Su, Ling-Yan,Li, Xue-Lian,Shen, Li,Zhang, Yue,Zhao, Meng-Meng,Yin, Zhi-Hua,Su, Hong-Ying,Zhou, Bao-Sen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Background: Telomerase reverse transcriptase (TERT) and cleft lip and palate trans-membrane 1 like (CLPTM1L) genes located on chromosome 5p15.33 are known to influence the susceptibility to various cancers. Here, we examined the association of TERT and CLPTM1L single nucleotide polymorphisms (SNPs) with hepatocellular carcinoma (HCC). Materials and Methods: Genotyping of TERT SNP rs2736098 and CLPTM1L SNP rs401681 was performed using TaqMan allelic discrimination assays in a case-control study of 201 HCC cases and 210 controls in a Chinese male population. Odds ratios (ORs) and 95% confidence intervals (CIs) were estimated using logistic regression analyses. Results: Both the rs2736098 T allele of TERT and the rs401681 T allele of CLPTM1L were associated with a significantly increased risk of HCC (adjusted odds ratio [OR]=1.605, 95% confidence interval [CI]=1.164-2.213; adjusted OR=1.399, 95%CI=1.002-1.955, respectively). Individuals carrying both TERT and CLPTM1L risk genotypes had an even higher risk of HCC (adjusted OR=4.420, 95%CI= 2.319-8.425). The TERT rs2736098 T allele was also significantly associated with the level of the HCC clinical indicator alpha-fetoprotein (P=0.026). Conclusions: Our results show that genetic variants of TERT and CLPTM1L may contribute to HCC susceptibility in Chinese males.

Path Coefficient Analysis on Major Silk Quality Characteristics of the Silkworm, Bombyx mori L.

( Qiao Ling Zhao ),( Long Li ),( Zhao Jun Wei ),( Zhi Fang Zhang ),( Xing Jia Shen ) 한국잠사학회 2002 International Journal of Industrial Entomology Vol.5 No.1