Localization of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal Antibody

Kang-seuk Choi,Jin-ju Nah,Young-joon Ko,Shien-young Kang,Yi-seol Joo 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal AntibodyKang-seuk Choi*, Jin-ju Nah, Young-joon Ko, Shien-young Kang1 and Yi-seok JooNational Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea1Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University, 48 Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk 361-763, KoreaReceived April 2, 2003 / Accept July 10, 2003J. Vet. Sci. (2003), 4(2), 167-173JOURNAL OFVeterinaryScience*Corresponding author: Kang-seuk Choi National Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea Tel: +82-31-467-1860, Fax: +82-31-449-5882 E-mail: choiks@nvrqs.go.kr

여대생의 결혼관에 관한 연구

강신미,김욱련,김이화,김현주,백은주 효성여자대학교 가정대학 학도호국단 1985 家政大論集 Vol.4 No.-

In these days, Korea has undergone various changes in every social aspect throughthe process of modernization. Especially in instituional and actual aspects, the family.and marriage have been changed greatly by the rapid urbanization and iudustrializatton,.and these changes have been investigated scientifically. This paper presents marital consciousness and actual behavior of 300 woman collegestudents in Daegu, and was surveyed comparing various parts-the humanities, natural·science and the arts. As for the basic attitude of marriage, they were positive in the need of marriage,,defined marriage as the second starting point of life. Also they put the aim of marriage,on mental value. As for the dating and purity before marriage, 84 percent of respondents assented tothe dating before marriage. And they thought it proper that the duration of datingwas one year to two years. The age of the first dating and the standard of sex moralstore significant. The part of the arts tended to be open, and the part of natural sciencewere conservative. As for the standard of spouse's choice, 84 percent of respondents wanted to have theright of priority they could choose their spouses for themselves. And they thought it·proper that the age of marriage was 25-year-old to 26-year-old, and the gap of maritalage was one year to three years. Ideal spouse's characteristic, home surroundings, and marital harmony were significant. Among various marital conditions, personality tended to to emphasized, and theatmosphere of family and masculine appearance were taken serious. As for a. spouses's job, professor or research occupation was popular. Compared withthe part of the arts, the part of the humanities and natural science were positive inpredicting marital harmony. Among various marital conditions, an only son, the eldest son, and an offspring ofa concubine tended to be evaded. The type of an ideal spouse was companionship.

식민지하 근대적 공장과 노동규율

姜怡守 尙志大學校 1997 論文集 Vol.18 No.-

This study on formations of 'modern subject' in the modern factory system under the Japanese colonial rule. Colonial industrialization has been changed since 1930' and industrial workers has increased rapidly. But adjusting to modern factory system was difficult to Korean workers who has accustomed to pre-industrial and irregular work habits. Japanese labor control method using an incentive system and strict rules had disciplined Korean workers. Under careful control by strict rules and encouraged by incentives, the Korean workers adjusted to the discipline of modern mechanized industry. And those disciplines produced colonial modern labourers as'modern subject'.

대농갱이 암,수의 성장 차이

강언종,이배익,조미영,손상규,김광석,김이청,방인철 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.4

대농갱이는 동자개과의 담수어로 고부가가치를 창출 할 수 있는 새로운 담수 양식 대상종으로 주목받고 있으나 현재까지 성장도 등 양식에 대한 기초정보가 밝혀지지 않았으며, 본 연구에서는 인공으로 생산한 자 ? 치어를 사육하면서 암 ? 수의 성장 차이를 비교 ? 조사하였다. 암수의 정확한 성장 차이를 구명하기 위해 본 실험은 자어 초기 조직검사에 의한 조사와 치어기부터 표지법에 의한 조사로 구분하여 실시하였다. 조직학적 판별에 의한 암수간 성장비교 시험 결과 부화 후 50일까지는 수컷과 암컷의 성장차이는 없는 것으로 나타났으나, 부화 80일 후에는 수컷(체장 72.0±6.7㎜, 체중 3.71±6.7g이 암컷(체장 64.3±6.7㎜, 체중 3.71±0.99g)보다 성장이 빠른 것으로 나타나 대농갱이는 부화 후 50일에서 80일 사이에 암 ? 수의 성장차이가 시작되는 것으로 사료되었다. 부화 80일 이후 조사는 표지법이 개체 성장에 미치는 영향을 조사한 후 본 실험에 착수하였으며 표지구는 대조구와 사이에 유으적인 성장의 차이를 보이지 않았다. 개체간 성장차이는 조직학적 판별 결과와 동일한 경향으로 수컷이 암컷보다 성장이 유의적으로 빨랐으며(P<0.05), 시험 종료 후 암컷과 수컷의 크기는 수컷이 평균 체장 17.57㎝, 체중 52.19g으로 암컷의 평균 체장 11.02㎝, 체중 16.26g보다 체장은 약 1.5배, 체중은 약 3배이상 큰 것으로 나타났다(p=0.05). 체중으로 본 성장곡선은 지수성장을 보였으며, 수컷은 Y=0.0024X^(1.738)(R^(2)=0.985), 암컷은 Y=0.0405X^(1.0316)(R^(2)=0.983)의 지수식에 밀접하게 수렵되었고 성장의 차이는 사육기간이 길어질수록 크게 나타났다. The Ussurien bullhead, Leiocassis ussuriensis, of the family Bagridae has recently been targeted for freshwater culture in Korea. In this paper we investigated sexual dimorphism in body size in rearing condition using specimens produced artificially. Tissue sections of gonads were made between 20 and 80 days after hatching to identify sex of the specimens investigated. A difference in body size between sexes was not observed in fry until they were 50 days old. Male fry 80 days old had attained 81.62f7.21 ㎜ total length, and females 72.84±8.62 ㎜ total length. After 80 days the difference in body size was analysed by tagging. Applying the tag by cutting fin tips had no affect on the growth of the fishes (P< 0.05). The growth in body size of males was significantly faster (P< 0.05) than in females, and males attained about 1.5 times more in standard length and about 3 times more in body weight than females at the end of 320 days. The growth curve of body weight was exponential and the body weight was expressed by Y = 0.0024X^(1.738) (R² = 0.985) and Y = 0.0405X^(1.0316)(R² = 0.983) in male and female, respectively, observed at monthly intervals.

鷄胎의 成長에 미치는 Nicotine의 影響에 關한 硏究

金利敏,金武剛 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

In order to study the effects of the nicotine in each organs of the developing animal embryos, auther selected. the mated chick eggs which is very easy to treat many samples at same time and hatched in the incubator at the condition of the 38±0.5℃ and 75±5% relative humidities. In the 6th day of the hatching the hatched eggs were punctured by the needle, and physiological saline as a control and nicotine solutions of 0.412mg, 0.825mg, or 1.650mg diluted in avian physiological solution were injected into the chorioallantoic membrane of the hatched eggs which were punctured by the needle as a treated group. The control and each treated groups were broken in the hatched day of the 12th, 14th, 16th, and 18th. The weights of each brain, heart, lung, liver and kidney of each group were measured by the torsion balance reading to the second point below the zero gram. After then the arithmetical mean and its standard deviation were calculated in each organs of each steps with the measured organs. In order to get the theoretical growth quantities, author applied the growth formula and calculated the error between the arithmetical mean and theoretical growth quantities, the percentage of the error to the growth quantity, mean error percentage, initial grpwth quantities, initial growth velocity, growth power, ratio of growth quantity, growth rate, and specific growth rate. The obtained results were summarized as followings. 1. The mean errors were below 5% in all organs only except the kidney of 1. 650mg injected group which was about 10%. 2. The growth quantities of the each organs were decreased according to increasing the injected volume of nicotine. 3. The comparative indices between the ratio of the growth quantities in nicotine treated group to the control group were larger in the treated lung organ. 4. Growth rates of each organs in coincidences of time lapse were increased in the brain and heart of the control, and brain, heart and lung of the 1. 650mg injected group but decreased in all organs of each treated group except the former. 5. Specific growth rate were increased in the lung of the 1. 650mg injected group, and the lung of the control group, kidney of the 0. 412mg injected group, and lung and liver of the 0.825mg injected group 'were increased at first and decreased after that, but all other organs in each group except above cases were decreased.

한국산야생등줄쥐(Apodemus agrarius coreae)의 해마복합체에서 neuropeptide Y 면역반응세포에 관한 연구

김무강,송치원,이경열,박일권,정영길,이강이,김종섭 충남대학교 수의과대학 동물의과학연구소 1997 動物醫科學硏究誌 Vol.5 No.-

Neuropeptide Y(NPY) was first isolated from porcine brain. This discovery has lead some workers to study the distribution of this peptide in the central nervous system of various mammals. In this study the distribution pattern of neuropeptide Y-immunoreactive neurons was examined in the hippocampal complex of Striped field mouse in Korea(Apodemus agrarius coreae) Experimental animals were anesthesized with thiopental sodium and perfused with 4% paraformaldehyde through the left ventricle and the ascending aorta. The brains were removed and transferred 10%, 20% and 30% sucrose solution. Sections were cut on a cryostat into 40㎛-thick. The tissue was immunostained with free-floating and avidin-biotm complex(ABC) method. The results obtained in this study were summarized as followings. 1. The most of NPY-IR neurons in hippocampus proper were found in the polymorphic layer and a few was found in the pyramidal layer. 2. The process run vertically in the deep polymorphic layer and run parallel to corpus callosum in superficial polymorphic layer. 3. The shapes of neurons in hippocampus proper were varied of polymorphic, oval or round, and the nerve fibers were found in the molecular laver and the hippocampal fissure. 4. The highest density in number of NPY-IR neurons on dentate gyrus were found in hilar area of dentate gyrus. 5. The most of NPY-IR nerve fibers were found in molecular cell layer.

肝蛭의 中間宿主貝 물달팽이와 애기물달팽이의 形態學的인 比較硏究 : lymnaea auricularia and L. Pervia

李康伊 中央醫學社 1990 中央醫學 Vol.55 No.12

Lymnaea auricularia and L. pervia are well known as intermediate host snails of Fasciola species in Korea. The distribution, habitat and the morphology difference of those were comparatively studies and summarized as follows; 1. L. auricular was collected in 113 places (53.1 %) and L. per via (36.6 %) in 78 places. Therefore, L. auricular was more largely distributed than L. per via and two species of Lymnaea were distributed mostly around the Keen river basin. 2. Among 213 regions which two species of Lymnaea lived, the habitat of L. auricularia was 113 places. Among them, there were 41 regions out of 51 regions of stream, 28 regions out of 38 regions of irrigable routes, 24 regions out of 26 rations of drainages and they lived in flowing streams. L. pervia was collected 53 regions out of 83 regions of paddy field and distributed in mostly still water. 3. In the morphological point of view, the body length and body width of L. auricularia were 17.76 + 1.34 mm x 11.98 ± 1.12 mm, those of L. pervia were 8.50 + 0.63 mm x 5.08= 0.44 mm. Therefore, L. auricularia was larger than L. pervia. The aperture of L. auricularia was wide ovate or globose, while L. pervia was an elongated oval type and the aperture length/body length of L. auricularia were 81.5 %, while those of L. pervia were less than 70 %; there was a remarkable difference between L. auricularia and L. Periva in the shell type of Lymnaea of two species. 4. For the radulla structure of the two species, there were no difference in central teeth and lateral teeth, while there was a little difference in marginal teeth. 5. In the structure of male copulatory organ, the penis sheath of L. auricularia was shorter than praeputium and sperm duct was rather slender and two muscular pillars were well developed. On the other hand, the length ratio between praeputium and penis in L. pervia varied. The muscular pillars were of the ordinary size and velum varied very much as to size. From the above results, among the intermediate host snails of Fasciola species, L. auricularia and L. pervia widly distributed in Korea showed remarkerable differences on the morphology and ecology of snails.

스트레스 사건이 활력증후에 미치는 영향에 관한 연구

이강이 中央醫學社 1991 中央醫學 Vol.56 No.6

This study was undertaken to assess the effect of stress on the vital sign. The subjects in this study were 101 patients who were examined the stomach disease with gastroscopy in C university hospital from April 24 to May 6, 1991. The vital sign with the effect of stress was taken three times. These cases were JBG (ust before gastroscopy), JAG (immediately after gastroscopy) and 30AG (30 minutes after gastroscopy). The data was analyzed by S.P.S.S. program using percentages, t-test, variance analysis, paired t-test and hottelings t-test. The results of study were as follows; 1. In analysis of the relation of vital sign with general characteristics, 1) Systolic and diastolic blood pressure: (1) Sex: In JBG, systolic and diastolic blood pressure were more elevated in female than male, but in IAG and 30 AG, male than female. There was significant difference between male and female in systolic and diastolic blood pressure in IAG. (2) Age: In JBG, systolic and diastolic blood pressure were more elevated in the old than the young, but in JAG, they showed opposite situation and in 30 AG, they were more elevated in the old than the young again. (3) Marital status: In JBG, IAG and 30 AG, systolic and diastolic blood pressure .were more elevated in the group of divorced and separated person than the married group, and there was significant difference between each group of marital status in diastolic blood pressure in IAG. 2) Pulse rate: (1) Sex: In JBG, IAG and 30 AG, the pulse rate was more increased in female than male and there was not significant difference between male and female in pulse rate. (2) Age: Pulse rate was more increased in the young than the old in all cases.

가정의 소득수준과 지각된 부모의 양육행동 및 학령기 아동의 자아정체감간의 관계

이강이 대한가정학회 2002 Human Ecology Research(HER) Vol.40 No.10

The purpose of this study was to examine the relation between level of income, perceived parental behaviors, and ego-identity of school-aged children. The subjects were 356 children in the 6th grade selected from six elementary schools in Seoul and Keunggi-Do. The subjects were asked to complete the questionnaire on ego-identity and perceived parental behaviors. The data was analyzed by Mean, SD, Cronbach's α, Pearson's correlation and Multiple Regression. The major findings were as follows: 1. The level of income(antecedent variable) was significantly related with perceived parental behavior(mediator) and ego-identity of school-aged children(dependent variable). 2. The effects of level of income on ego-identity of school-aged children became lower after control of the effects of perceived parental behaviors. In other words, perceived parental behaviors partially mediated the effects of level of income on ego-identity of school-aged children.

마늘의 효능과 안전성에 관한 연구

이애정;이상화;조강진;김인혜;엄애선 한양대학교 2010 韓國 生活 科學 硏究 Vol.30 No.1

Galic is the fresh or dehydrated bulb obtained from Allium scorodorpasum var. viviparum Regel, a genus of the lily family. Garlic has been widely used as oriental medicine having various health benefits, such as cardiovascular, antineoplastic, antimicrobial and antiinflammatory. The average daily intake in western people is 4g/day of fresh garlic whereas it in Koreans 5.8g/day. However, consumption of excessive amount of raw garlic can cause gastrointestinal disease, dermatitis, asthma, allergic reaction, anticoagulant action, breath and body odor. Additionally, its antithrombotic properties prompt patients to stop taking garlic 14 days before having surgery or dental work. Therefore, we reviewed the safety of garlic on the basis of literatures published and then further studies on the safe amount of garlic consumption should be considered.