PKD2 interacts with Lck and regulates NFAT activity in T cells

( Qing Li ),( Xiao Qing Sun ),( Jun Wu ),( Zhi Xin Lin ),( Ying Luo ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.1

No Association Between the USP7 Gene Polymorphisms and Colorectal Cancer in the Chinese Han Population

Li, Xin,Wang, Yang,Li, Xing-Wang,Liu, Bao-Cheng,Zhao, Qing-Zhu,Li, Wei-Dong,Chen, Shi-Qing,Huang, Xiao-Ye,Yang, Feng-Ping,Wang, Quan,Wang, Jin-Fen,Xiao, Yan-Zeng,Xu, Yi-Feng,Feng, Guo-Yin,Peng, Zhi-Ha Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Colorectal cancer (CRC), now the third most common cancer across the world, is known to aggregate in families. USP7 is a very important protein with an important role in regulating the p53 pathway, which is critical for genomic stability and tumor suppression. We here genotyped eight SNPs within the USP7 gene and conducted a case-control study in 312 CRC patients and 270 healthy subjects in the Chinese Han population. No significant associations were found for any single SNP and CRC risk. Our data eliminate USP7 as a potential candidate gene towards for CRC in the Han Chinese population.

Characteristics of circRNAs expression profiles in the piglets intestine induced by oxidative stress

Li Zhi-xin,Chen Wei,Qin Ming,Wang Li-xue,Zeng Yong-qing 한국유전학회 2022 Genes & Genomics Vol.44 No.4

Backgroud: Oxidative stress (OS) can affect the expression of key genes and destroy the intestinal structure. However, it is unclear how OS regulates the expression of circular RNAs (circRNAs), microRNAs (miRNAs) and mRNAs. Objective: The aim of this study was to examine the expression of circRNAs, miRNAs and mRNAs exposed to OS. Methods: Piglets were exposed to diquat (DQ), a herbicide, and the activity of antioxidant enzymes and the morphology of the intestine were investigated. We utilized whole transcriptome sequencing to examine the global expression of circRNAs, miRNAs and mRNAs in the jejunum. Results: Compared to controls, 751 circRNAs, 731 miRNAs and 164 mRNAs were differentially expressed in diquat-treated piglets. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that oxidative phosphorylation, RNA degradation and ubiquitin-mediated proteolysis were closely associated with OS. Conclusions: Our results indicated that diquat-induced OS alters the intestinal structure, resulting in the differential expression of circRNAs, miRNAs and mRNAs in the jejunum of piglets. Meanwhile, OS weakened the enzyme antioxidant system in serum of piglets. Our results provide a foundation for further studies on the mechanisms involved in the response to OS in the jejunum.

Thalidomide Combined with Chemotherapy in Treating Patients with Advanced Colorectal Cancer

Huang, Xin-En,Yan, Xiao-Chun,Wang, Lin,Ji, Zhu-Qing,Li, Li,Liu, Meng-Yan,Qian, Ting,Shen, Hui-Ling,Gu, Han-Gang,Liu, Yong,Gu, Ming,Deng, Li-Chun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.17

Objective: To assess the safety and effectiveness of thalidomide (produced by CHANGZHOU PHARMACEUTICAL FACTORY CO.LTD) combined with chemotherapy in treating patients with advanced colorectal cancer. Method: A consecutive cohort of pretreated patients with advanced colorectal cancer were treated with thalidomide combined with chemotherapy. And chemotherapy for patients with advanced colorectal cancer were administered according to the condition of patients. Thalidomide was orally administered at a dosage of 50mg/day to 150mg/day before sleeping for at least 14 days. After at least 14 days of treatment, safety and side effects were evaluated. Results: There were 12 female and 3 male patients with advanced cancer recruited into this study, including 9 patients with colon, 6 patients with rectal cancer. The median age of patients was 57(41-82) years. Partial response was observed in 2 patients (2/15), and stable disease in 3 patients(3/15). Incidences of Grade 1 to 2 myelosuppression was observed in 1/15 patients, and Grade 1 to 2 elevation of hepatic enzyme was recorded in 1/15 patients. Adverse effects on the gastrointestinal tract were documented in 1/15 patients, and were Grade 1. No Grade 3-4 toxicities were diagnosed. No treatment related death was found. Conclusions: Thalidomide combined with chemotherapy was safe and mildly effective in treating patients with advanced colorectal cancer. However, further study should be conducted to clarify the effectiveness of this combination.

Construction and effect of intramolecular hydrogen bond on solvent resistance of polymeric membranes and their application in impermeable membranes

Xiaoyun Li,Zhen Wang,Xin Jing,Xudong Li,Junwei Wang,Mao-qing Kang,Yuhua Zhao,Qifeng Li 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.107 No.-

Hydrogen bonds, which can be divided into many types along with the deepening of research, exist inmany compounds and have certain impact on properties of substances. In this work, effect of intramolecularhydrogen bond on solvent resistance of polymeric membranes was studied from two aspects. On theone hand, the existence of intramolecular hydrogen bond on solvent resistance of polymeric membraneswas studied by constructing and shielding special hydroxyl groups in typical polymer polyurethane. Onthe other hand, content of intramolecular hydrogen bond on solvent resistance was investigated by buildingdifferent ratios of inter- and intra-molecular hydrogen bonds in polyvinyl alcohols. The structures,solvent resistance and hydrogen bonding interactions were systematically investigated by FT-IR, XRD,Ds and performances before and after immersed in solvents. Permeabilities of the membranes for practicalapplication were also characterized. The results showed that distinct type and ratio of hydrogenbonds played different role on solvent resistance. A good agreement between the swelling performancesand content of intramolecular hydrogen bond proved the positive effect of intramolecular hydrogen bondon solvent durability of polymeric membranes. In addition, polyurethane membranes exhibited goodimpermeability to water vapor, oxygen and chloride ions which showed potential application in manyfields.

Streptomyces xinghaiensis sp. nov., isolated from marine sediment.

Zhao, Xin-Qing,Li, Wen-Jun,Jiao, Wen-Ce,Li, Yan,Yuan, Wen-Jie,Zhang, Yu-Qin,Klenk, Hans-Peter,Suh, Joo-Won,Bai, Feng-Wu Society for General Microbiology 2009 International journal of systematic and evolutiona Vol.59 No.11

<P>A novel actinomycete, strain S187(T), was isolated from a marine sediment sample collected from Xinghai Bay, Dalian, China. Growth occurred on ISP medium 2 containing 0-9 % NaCl and at pH 6.0-9.0 and 10-45 degrees C. The cell wall of strain S187(T) contained the isomer ll-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H(6)) (40.8 %), MK-9(H(8)) (38.2 %) and MK-9(H(2)) (8.8 %). The major fatty acids were iso-C(16 : 0) (29.6 %), anteiso-C(15 : 0) (14.0 %) and anteiso-C(17 : 0) (11.6 %). Cells contained phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannosides and one unknown phospholipid. The G+C content of the genomic DNA was 72.01 mol%. The 16S rRNA gene sequence of the isolate had similarities of 98.1 and 97.5 % with those of Streptomyces flavofuscus NRRL B-8036(T) (=DSM 41426(T)) and Streptomyces albiaxialis DSM 41799(T), respectively, showing that the novel strain should be assigned to the genus Streptomyces. DNA-DNA hybridizations with the two above-mentioned Streptomyces species showed 31.4 and 46.9 % relatedness, respectively. Moreover, the three strains differed in some physiological and biochemical properties. Thus, on the basis of phenotypic and genotypic analyses, it is proposed that strain S187(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces xinghaiensis sp. nov. is proposed; the type strain is S187(T) (=NRRL B-24674(T)=CCTCC AA 208049(T)=KCTC 19546(T)).</P>

Screening for MiRNAs Related to Laryngeal Squamous Carcinoma Stem Cell Radiation

Huang, Chang-Xin,Zhu, Ying,Duan, Guang-Liang,Yao, Ji-Fen,Li, Zhao-Yang,Li, Da,Wang, Qing-Qing Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

Objective: To use microarray chip technology for screening of stem cell radiation related miRNAs in laryngeal squamous cell carcinoma; study and explore the relationship of miRNAs with radiosensitivity of laryngeal squamous cells. Method: After conventional culture and amplification of the laryngeal squamous carcinoma cell line Hep-2, CD 133+ cells were screened out with combination of isolated culture of stem cell microspheres and FACS for preparation of laryngeal cancer stem cells. After radiation treatment, miRNAs of laryngeal squamous carcinoma stem cells before and after radiation were enriched and purified. After microarray hybridization with mammalian miRNA and scanning of fluorescence signal, the miRNAs of laryngeal squamous carcinoma stem cells before and after radiation was subject to differential screening and clustering analysis. Real-time quantitative RT-PCR was used to verify part of the differentially expressed miRNAs. Results: 70 miRNAs related to laryngeal cancer stem cell radiation with 2-fold difference in expression were screened out, in which 62 were down-regulated and 8 were up-regulated. Fluorescent quantitative RT-PCR results were consistent with miRNAs chip results. Conclusion: Some miRNAs may be involved in self-regulation with laryngeal squamous carcinoma stem cell radiation.

Hydrogen peroxide is involved in abscisic acid-induced adventitious rooting in cucumber (Cucumis sativus L.) under drought stress

Xue-Ping Li,Qing-Qing Xu,Wei-Biao Liao,Zhan-Jun Ma,Xiao-Ting Xu,Meng Wang,Peng-Ju Ren,Li-Juan Niu,Xin Jin,Yong-Chao Zhu 한국식물학회 2016 Journal of Plant Biology Vol.59 No.5

Abscisic acid (ABA) and hydrogen peroxide (H2O2) are important regulatory factors involved in plant development under adversity stress. Here, the involvement of H2O2 in ABA-induced adventitious root formation in cucumber (Cucumis sativus L.) under drought stress was determined. The results indicated that exogenous ABA or H2O2 promoted adventitious rooting under drought stress, with a maximal biological response at 0.5 μM ABA or 800 μM H2O2. The promotive effects of ABA-induced adventitious rooting under drought stress were suppressed by CAT or DPI, suggesting that endogenous H2O2 might be involved in ABA-induced adventitious rooting. ABA increased relative water content (RWC), leaf chlorophyll content, chlorophyll fluorescence parameters (Fv/Fm, ΦPS II and qP), water soluble carbohydrate (WSC) and soluble protein content, and peroxidase (POD), polyphenol oxidase (PPO) and indoleacetate oxidase (IAAO) activities, while decreasing transpiration rate. However, the effects of ABA were inhibited by H2O2 scavenger CAT. Therefore, H2O2 may be involved in ABA-induced adventitious root development under drought stress by stimulating water and chlorophyll content, chlorophyll fluorescence, carbohydrate and nitrogen content, as well as some enzyme activities.

Knockdown of HMGN5 Expression by RNA Interference Induces Cell Cycle Arrest in Human Lung Cancer Cells

Chen, Peng,Wang, Xiu-Li,Ma, Zhong-Sen,Xu, Zhong,Jia, Bo,Ren, Jin,Hu, Yu-Xin,Zhang, Qing-Hua,Ma, Tian-Gang,Yan, Bing-Di,Yan, Qing-Zhu,Li, Yan-Lei,Li, Zhen,Yu, Jin-Yan,Gao, Rong,Fan, Na,Li, Bo,Yang, Jun Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

HMGN5 is a typical member of the HMGN (high mobility group nucleosome-binding protein) family which may function as a nucleosomal binding and transcriptional activating protein. Overexpression of HMGN5 has been observed in several human tumors but its role in tumorigenesis has not been fully clarified. To investigate its significance for human lung cancer progression, we successfully constructed a shRNA expression lentiviral vector in which sense and antisense sequences targeting the human HMGN5 were linked with a 9-nucleotide loop. Inhibitory effects of siRNA on endogenous HMGN5 gene expression and protein synthesis were demonstrated via real-time RT-PCR and western blotting. We found HMGN5 silencing to significantly inhibit A549 and H1299 cell proliferation assessed by MTT, BrdU incorporation and colony formation assays. Furthermore, flow cytometry analysis showed that specific knockdown of HMGN5 slowed down the cell cycle at the G0/G1 phase and decreased the populations of A549 and H1299 cells at the S and G2/M phases. Taken together, these results suggest that HMGN5 is directly involved in regulation cell proliferation in A549 and H1299 cells by influencing signaling pathways involved in cell cycle progression. Thus, our finding suggests that targeting HMGN5 may be an effective strategy for human lung cancer treatment.

Biomechanical Role of Nucleotomy in Vibration Characteristics of Human Spine

Qing-Dong Wang,Li-Xin Guo 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.7

Nucleotomy is a common surgical procedure for the treatment of lumbar diseases. It may accelerate degeneration in the operated disc and decreased segmental stability, and this has been widely concerned by scholars for many years. However, under whole-body vibration, nucleotomy how to affect the vibration characteristics of the lumbar spine and complications is urgent to know. A three-dimensional nonlinear osteoligamentous finite element model of the intact L1-sacrum lumbar spine with muscles was established, and the nucleus of the L4–L5 disc was removed in the nucleotomy model. The lower surface of the sacrum was fully constrained for all models. A 5 Hz, 40 N sinusoidal vertical load supplemented with a 400 N preload was applied at L1 to simulate the vertical vibration of the human body. The results showed that nucleotomy increased the dynamic responses of the discs such as stress in the annulus ground substance and intradiscal pressure both in the maximum value and vibration amplitude. The maximum endplate stresses and corresponding vibration amplitudes of the denucleated L4–L5 level increased because of nucleotomy. Nucleotomy decreased the maximum response values of disc height and segmental lordosis but increased the corresponding amplitudes. Therefore, these findings imply that nucleotomy may increase the risk of developing complications such as disc degeneration, adjacent segment disease, endplate degeneration, lumbar instability, nerve root compress, isthmic spondylolisthesis, and lumbar disc herniation under whole-body vibration. This study reveals insights into the effect of the nucleotomy on the vibration characteristics of the lumbar spine and provides new information toward the relationship between nucleotomy and complications.