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- 저자 : Lei Mao (검색결과 38 건)
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Lei Gao,Feng Li,Ye Wang,Xing Mao Xiao,Peng Da Huo 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.8
Solid-state bonding of heterogeneous materials is one of the effective ways to achieve light weight. An extrusion formingprocess for Mg/Al thickness-oriented bonding sheet was carried out. Due to triaxial compressive stress in the extrusion container,the AZ31 and AA6061 billets were deformed and the bonding and forming of Mg/Al thickness-oriented sheet can berealized by this method. The observation results of the bonding position by using XRD, SEM and EDS showed that: at theforming temperature with 360–420 °C, it had a good bonding interface, and the width of the transition layer increased withthe increase of the forming temperature. The presence of brittle intermetallic compounds in the transition layer indicated thatmetallurgical bonding had occurred at the interface. Considering all factors comprehensively, when the forming temperaturewas 390 °C, the bonding quality was appropriate. Its tensile-shear strength was 42 MPa, the width of the interface transitionlayer was about 10 μm, and the microhardness was about 164.3 HV. This method provided scientific guidance and technicalreserves for green forming of Mg/Al sheets.
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Lei Mao,Fei Wang,Yuanyuan Li,Yufeng Dai,Yanjun Liu,Jingfeng Wang,Changhu Xue 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.2
Glucocorticoids are the leading cause of secondaryosteoporosis. In the current study, the in vivoeffects of Antarctic krill (Euphausia superba) oil (AKO) ondexamethasone-treated mice were investigated. Resultsshowed that AKO significantly prevents bone loss, asevidenced by improved bone mineral density, biomechanicalstrength, and cancellous bone microstructure. Fluorescencedouble-labeling of femur showed that AKOinduces new bone formation. Toluidine blue staining ofmarrow cavity indicated that AKO increases the number oftrabecula, and decreases the generation of adipose cells. Runt-related transcription factor 2 (Runx2) and Peroxisomeproliferator-activated receptor c (PPARc) are the switchesfor osteogenic and adipogenic differentiation of bonemarrow mesenchymal stem cells, respectively. AKO significantlypromoted the expression of Runx2 protein, andreduced PPARc expression in bone tissue. Furthermore,AKO increased the mRNA expression of osteogenesis-relatedgenes and decreased the expression of adipogenesisrelatedgenes. In conclusion, AKO improved glucocorticoid-induced osteoporosis via promoting bone formation.
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Mao, Zhu,Liu, Zhuo,Chen, Lei,Yang, Jin,Zhao, Bing,Jung, Young Mee,Wang, Xu,Zhao, Chun American Chemical Society 2013 ANALYTICAL CHEMISTRY - Vol.85 No.15
<P>SERRS (surface-enhanced resonance Raman scattering) has been used to develop and optimize a novel and quantitative MTT assay for living cell viability. This highly sensitive method derives from two factors for formazan signal enhancing: the addition of Au nanoparticles and the resonance effect by 632.8 nm of excitation. The results show that the background elements, such as excessive MTT residues, serum, and the drug, did not interfere with the detection of formazan. Moreover, the detection limit of formazan is as low as 1 ng/mL. With the use of this method to quantify metabolically viable cells, dose–response curves of treated and untreated cells with the drug were constructed on the human lung cancer cell A549. The results also show that the Raman signal generated is dependent on the degree of activation of the cells. In comparison to the traditional method, the main advantages of this method are its rapidity (30 min), high-selectivity, high-precision, and cost-effectiveness (0.1 mg/mL MTT) without time-consuming steps and any modifying or labeling procedure. This work reports on an improved research tool that may help researchers apply this method for in situ cell assays.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2013/ancham.2013.85.issue-15/ac401254s/production/images/medium/ac-2013-01254s_0010.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac401254s'>ACS Electronic Supporting Info</A></P>
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Substructure based structural damage detection with limited input and output measurements
Lei, Y.,Liu, C.,Jiang, Y.Q.,Mao, Y.K. Techno-Press 2013 Smart Structures and Systems, An International Jou Vol.12 No.6
It is highly desirable to explore efficient algorithms for detecting structural damage of large size structural systems with limited input and output measurements. In this paper, a new structural damage detection algorithm based on substructure approach is proposed for large size structural systems with limited input and output measurements. Inter-connection effect between adjacent substructures is treated as 'additional unknown inputs' to substructures. Extended state vector of each substructure and its unknown excitations are estimated by sequential extended Kalman estimator and least-squares estimation, respectively. It is shown that the 'additional unknown inputs' can be estimated by the algorithm without the measurements on the substructure interface DOFs, which is superior to previous substructural identification approaches. Also, structural parameters and unknown excitation are estimated in a sequential manner, which simplifies the identification problem compared with other existing work. Structural damage can be detected from the degradation of the identified substructural element stiffness values. The performances of the proposed algorithm are demonstrated by several numerical examples and a lab experiment. Measurement noise effect is considered. Both the simulation results and experimental data validate that the proposed algorithm is viable for structural damage detection of large size structural systems with limited input and output measurements.
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Large Eddy Simulation of Fuel Sprays in Diesel Engines
( Mao Zhao Xie ),( Ming Jia ),( Lei Zhou ) 한국액체미립화학회 2010 한국액체미립화학회 학술강연회 논문집 Vol.2010 No.-
A brief introduction of the recent research at Dalian University of Technology (DUT) on the applications of the large eddy simulation (LES) approach to fuel sprays in a constant volume vessel and in a real engine configuration is presented. Firstly the fundamental of the LES approach and related mathematical models are briefly described. Then, some aspects concerning applications of LES to the fuel spray in the engine environment are discussed, including comparisons between RANS (Reynolds Averaged Navier-Stokes equations) and LES models; an examination of the influence of different subgrid scale (SGS) models on fuel sprays and their applicability to IC engines; a performance assessment of four breakup models in connection with the LES simulations; as well as an example of the LES application to a Ford 2.0 liter high-speed direct-injection (HSDI) diesel engine. Finally, suggestions to the further LES study in the engine area are proposed.
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Substructure based structural damage detection with limited input and output measurements
Y. Lei,C. Liu,Y.Q. Jiang,Y.K. Mao 국제구조공학회 2013 Smart Structures and Systems, An International Jou Vol.12 No.6
It is highly desirable to explore efficient algorithms for detecting structural damage of large size structural systems with limited input and output measurements. In this paper, a new structural damage detection algorithm based on substructure approach is proposed for large size structural systems with limited input and output measurements. Inter-connection effect between adjacent substructures is treated as „additional unknown inputs‟ to substructures. Extended state vector of each substructure and its unknown excitations are estimated by sequential extended Kalman estimator and least-squares estimation, respectively. It is shown that the „additional unknown inputs‟ can be estimated by the algorithm without the measurements on the substructure interface DOFs, which is superior to previous substructural identification approaches. Also, structural parameters and unknown excitation are estimated in a sequential manner, which simplifies the identification problem compared with other existing work. Structural damage can be detected from the degradation of the identified substructural element stiffness values. The performances of the proposed algorithm are demonstrated by several numerical examples and a lab experiment. Measurement noise effect is considered. Both the simulation results and experimental data validate that the proposed algorithm is viable for structural damage detection of large size structural systems with limited input and output measurements.
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Li Mao-Ye,Huang Yan,Lei Xiao,Xu Chuan-Tao,Li Bin,Chen De-Xin,Liu Su 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3
Heat shock proteins (HSPs) play essential roles in stress tolerance in insects, such as the cigarette beetle, Lasioderma serricorne, which is an important pest of stored products. However, the function of HSPs in envi ronmental stress response in L. serricorne is poorly understood. In this study, six cDNAs encoding putative HSP70s were identified in L. serricorne (LsHSP70-1 to LsHSP70-6). The LsHSP70 proteins identified have signature motifs of insect HSP70s and exhibit high amino-acid identity with their respective orthologs from other insect species. Phylogenetic analysis showed that these LsHSP70s fall into three major clans: HSP70 in the cytosol (four genes), endoplasmic reticulum (one gene), and mitochondria (one gene). LsHSP70 genes are differentially expressed in different developmental stages. LsHSP70-4 and LsHSP70-5 are mainly expressed at the larval stage, while LsHSP70-3 shows the highest level at the pupal stage. The other genes are ubiquitously expressed. Furthermore, the expression levels of LsHSP70-1 and LsHSP70-4 are significantly upregulated upon exposure to temperatures of both 0 and 15 ◦ C, while LsHSP70-3 is inducible at 15 ◦ C and LsHSP70-6 at 0 ◦ C. In addition, treatment at 0 ◦ C causes significant downregulation of LsHSP70-2 and LsHSP70-3. Exposure to a temperature of 30 ◦ C upregulates LsHSP70-1 expression, while 35 and 40 ◦ C treatments result in significantly enhanced transcription of all the LsHSP70 genes. To the best of the authors’ knowledge, this is the first report on the sequence characteristics, phylogenetic relationships, and expression profiles of HSP70 genes in L. serricorne. The cold- and heat-inducible regulation of LsHSP70s suggests that these genes are related to tolerance of abnormal temperatures.
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Chen, Lei,Seo, Hyeong Kuyn,Mao, Zhu,Jung, Young Mee,Zhao, Bing Royal Society of Chemistry 2011 Analytical methods Vol.3 No.7
<P>We introduce a new approach that combines the self-assembly and seeding methods for silver plating of the proposed Fe<SUB>2</SUB>O<SUB>3</SUB>@Au-seed substrate. By adjusting the silver plating time between 0 and 360 s, the optical resonance of the substrates clearly varied from visible to near-infrared. To investigate the potential of these substrates for use in surface-enhanced Raman scattering (SERS) applications, SERS spectra of thiophenol (TP), 4-aminothiophenol (PATP) and rhodamine 6G (R6G) were evaluated at three excitation wavelengths (532, 633 and 785 nm). Analysis of the SERS spectra clearly demonstrated that the SERS effect depends strongly on the nature of the substrate surface and the nature and electronic resonance of the probe molecules. The calculated enhancement factor (EF) of TP adsorbed onto an Fe<SUB>2</SUB>O<SUB>3</SUB>@Ag substrate was ∼10<SUP>7</SUP> following three laser excitations. More importantly, these SERS substrates can successfully be utilized for the detection of small molecules at very low concentrations (∼100 pg mL<SUP>−1</SUP>).</P> <P>Graphic Abstract</P><P>The optimal Fe<SUB>2</SUB>O<SUB>3</SUB>@Ag substrate was employed for protein and small molecule recognition assay. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0ay00729c'> </P>
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