Staphyloccus aureus에 대해 gentamicin의 MIC측정을 위한 firefly Iuciferase assay의 응용

이승배,최상훈,이동만 건국대학교 1987 論文集 Vol.24 No.1

Susceptibllity of Staphyloccus aureus (ATCC 14492) to gentamicine was determined by minium inhibitory concentration(MIC) test and firefly bioluminescence assay of bacterial adenosine triphosphate(ATP). ATP content extracted Staphyloccus aureus was proportional to the number of bacterial, ATP content in a bacteria was 3.1fmol. The patterns of growth curves for Staphyloccus aureus look very similar, when the bacterial growth was monitored by ATP content and viable cell count. The MIC of gentamicine for Staphyloccus aureus measured both firefly bioluminescence assay and MIC test was 0.5ug. Determinent of intrcellular ATP in culture of Staphyloccus aureus exposed to gentamicin seem to be suitable for reliable determination of MIC within 3 hours of incubation. It gave very good correlation(r=0.97), when firefly bioluminescence and MIC test were compared.

만경강 본류의 자연정화능 향상을 위한 식생학적 진단

이경보,김창환,김종구,이덕배,박찬원,나승용 한국환경농학회 2003 한국환경농학회지 Vol.22 No.2

만경강 본류를 대상으로 수질을 개선하기 위한 일환으로 하천식생의 종구성, 하천형태에 따른 군락의 구조, 단면분석을 통한 현존식생의 분포 등을 종합적으로 분석하여 하천 생태계에 대한 종합적 해석과 식생에 의한 자연정화능 활용방안을 평가하고자 2001년 6월부터 2002년 3월까지 수질 및 식생에 관한 자연정화능을 조사, 평가하였다. 만경강 본류 수질중 T-N의 농도는 갈수기인 겨울철이 17.23 mg/L로 가장 높았으며, 여름철이 8.59 mg/L로 낮았고, 상류보다는 하류에서 그 농도가 높았다. 또한 평균 T-P의 농도는 여름철과 가을철에 각각 0.45, 0.47 mg/L로 비슷하였으나 겨울철에 1.79 mg/L로 농도가 높아졌다. BOD의 농도는 상류유역에서 0.95~2.57 mg/L로 Ⅰ~Ⅱ급수 수질을 나타내었으나 하류유역에서는 6.87~9.72 mg/L로 Ⅴ급수에 가까운 수질이었다. 만경강 상류의 식물상은 73과 134속 218종 33변종으로 총 251종류가 조사되었으며, 중류 일대에서 조사된 식물은 33과 69속 82종 16변종으로 총 98종류가 조사되었다. 그리고 만경강 하류 일대에서 관찰된 식물은 27과 58속 71종 14변종으로 총 85종류가 조사되었다. 만경강 유역에서 자연정화능에 의한 수질개선을 도모하기 위해 식물 생활형별로 하천 정화능이 뛰어난 식물을 조사 분석한 결과 침수식물은 붕어마름, 검정말, 부엽식물은 어리연꽃, 왜개연꽃, 추수식물은 갈대, 줄, 부들, 습생식물은 큰황새 냉이, 고마리 등이 질소, 인 흡수능이 우수한 식물이었다. This study was conducted to get some information on plants abilities to enhance water purification and to find out away to conserve the ecosystem in Mankyeong river. Vegetation were surveyed at 4 sites pointing by 1:5,000 topographical map, from June 2001 through March 2002. T-N content in water were high in all sites of Mankyeong river, the average T-N levels were 8.59 and 17.23 ㎎/L summer and winter, respectively. The average T-P level during summer was 0.47 ㎎/L, but that was 1.79 ㎎/L during winter. The BOD level in Mankyeong upstream ranged from 0.95 to 2.57 ㎎/L which would be in Ⅰ or Ⅱ grade according to water quality criteria by Ministry of Environment, but BOD level in Mankyeong downstream ranged from 6.87 to 9.72 ㎎/L. The plant species of river flora were found 251, 98 and 85, upstream, midstream and down stream, respectively. Among the surveyed plants, Ceratophyllum demersum, submerged plant and Nuphar subinteperrimum took up higher contents of phosphate and nitrogen than other plants. The Phragmites communis and Zizania latifolia having much biomass were thought to be suitable plants for enhancement of the natural water purification.

만경강 상류 수질 및 식생분포와 토양환경에 따른 하천식생의 종 다양성

이경보,김창환,이덕배,김종구,박찬원,나승용 한국환경농학회 2003 한국환경농학회지 Vol.22 No.2

만경강 상류유역 수질 및 하천식생 군락 변화를 조사 분석하여 수질에 따른 하천식생의 종 다양성 및 토양요인에 따른 종 다양성의 변화를 평가하기 위하여 2001년 6월부터 2002년 9월까지 만경강 상류유역 5개 조사지점을 선정한 후 조사를 실시하였다. 수질중 T-N의 농도는 전주천과 삼천에서 높았으며, 겨울철에 전주천에서 7.45 mg/L로 가장 높았다. 무기태 질소 성분벽 함량은 NH₄-N 경우 고산천과 소양천 유역은 0.01~0.06 mg/L 범위로 계절별 커다란 차이가 없는 낮은 농도를 나타냈으나, 생활하수가 유입되는 전주천과 삼천에서는 0.77~3.01 mg/L로 계절별로 큰 차이를 보였다. T-P의 농도는 계절별로 비슷하였으나 전주천에서 겨울천에 0.89 mg/L로 가장 높았다. 계절별 BOD의 농도는 고산천과 소양천 유역에서 0.92~2.14 mg/L를 나타내었으나 생활하수가 유입되는 유역에서는 2.01~7.65 mg/L범위를 보였다. SO₄²^(-)의 농도는 농업용수 수질기준인 50 mg/L를 초과하는 지역은 없었으며 소양천에서 다소 높았다. 만경강 지류인 고산천에서 조사된 식물은 59과 과 129속 165종 20변종으로 총 185종류가 조사되었으며, 소양천에서 조사된 식물은 53과 111속 141종 19변종으로 총 160종이었고, 전주천 하류에서 조사된 식물은 37과 68속 86종 15변종으로 총 103종이었다. 그리고 삼천에서 조사된 식물은 32과 92속 110종 18변종으로 총 128종류 조사되었으며, 만경강 본류인 하리유역에서 조사된 식물은 73과 134속 218종 33변종으로 총 251종류가 조사되었는데 식물의 생활형에 따라 분류하면 침수식물이 13종, 부엽식물이 5종, 부유식물이 2종, 추수식물이 26종, 수생식물이 46종, 습생식물이 47종으로 조사지점중 이 지역의 식생이 가장 다양하였다. 식물사회학적 방법에 따라 분류된 하천식생의 식물군락은 10개의 수생식물 군락과 2개의 습생 및 수변식물 군락으로 대별되어졌다. 이들 군락들에 대한 4종류(종수, 종의 풍부도지수, 종의 이질성지수, 종의 균등도지수)의 종 다양성과 토양요인(pH, EC, 유기물 함량, 전질소, 인삭)과의 관계를 분석한 결과 pH가 높을수록 4종류의 종 다양성 지수는 높아 졌으며, EC는 값이 높을수록 종수(SN), 종의 풍부도지수(SR), 종의 이질성지수(H'), 종의 균등도지수(J')가 낮아 졌다. 또한 유기물 함량(OM)과 전질소(T-N)의 경우도 4종류의 다양성지수와는 부의 상관관계를 나타내었다. 인산(P₂O_(5))은 4종류의 다양성 지수의 변화에 영향을 미치지 않았다. This study was conducted to evaluate influence of chemical properties in the riparian on the species diversity and to get plant information for enhancement of natural purification in Mankyeong River. The concentration of total nitrogen was high in Jeonju and Sam stream, while that of total nitrogen showed the highest peak in Winter. Concentrations of NH₄-N was 0.01-0.06 ㎎/L in Gosan and Soyang stream. The water quality of upstream along with Mankyeong River was suitable for the imgation source. The riparian vegetation was investigated by Zurich-Montpellier school's method from June, 2001 to September, 2002. The number of riparian plants were 59 families, 129 genera, 165 species, 20 varieties in Gosancheon, on the while 53 families, 111 genera, 141 species, 19 varieties in Soyangcheon The number of riparian plants in Hari basin was higher than that of other sites namely, 73 families, 134 genera, 218 species, 33 varieties. Riparian vegetation was consisted of 12 plant communities. The contents of organic matter, total nitrogen and electrical conductivity had negative relationship with species diversity (Species richness index, Heterogeneity index, Species evenness index, Species number). On the while, species diversity had positive relationship with soil pH. Species diversity of the plant communities were affected by topography and disturbance.

Bacillus thuringiensis 아종 Kustaki의 내독소 결정체에 대한 항체생산과 항혈철중 면역글로부린 G의 분리

이승배,김창규 건국대학교 1986 論文集 Vol.23 No.1

The antisera were obtained by inoculating rabbits with purified solublized crystal toxins from Bacillus thuringiensis subspp. Kustaki HD1 and HD73. Enzyme-linked Immunosorbent Assay(ELISA) was used to detect anti crystal toxin antibody. Bacillus thuringiensis subspp. kutaki and HD73 crystal toxins were purified by Renografin-76 density gradient centrifugation. The density of crystal toxin was 1.22g/cm3. When crystal toxins were infected into rabbits for producing anti-crystal toxin antibody, the good results were obtained at 4 or 5 times injections with 50 to 200og crystal toxin. Immunoglobulin G in antisera was purified by Protein-A Affinity Chromatography. The heavy chain and the light chain of immunoglobulin G were seperated by SDS-polyacrylamide gel electrophoresis. Then molecular weights of heavy chain and light chain were approximately 50,000 daltons and 25,000 daltons.

Bioluminescence 방법에 의한 우유 및 유제품에 존재하는 세균수 측정에 관한 연구

이승배,도형기 건국대학교 1986 論文集 Vol.22 No.1

Determination of bacterial ATP based on luciferin-luciferase bioluminescence reaction was applied to the measurement of bacteria in raw milk and liquid yoghurt as in alternative rapid method. The results are as follows : 1. The light yield was proportional to the concentration of ATP giving a straight light within a range of 10-10 to 10-6M. 2. The light yield related with the ATP content was increased as the number or bacteria in E. coli and L. bulgaricus was increased. When ATP content of bacteria were determined by bioluminescence reaction and compared. 3. The patterns of growth curve for E. coli look very similar, when they were monitored by ATP content assayed by bioluminescence reaction and total colony count. 4. The bacterial ATP of each sample in raw milk and liquid yoghurt was proportional to the number of bacteria, Coefficient of correction was 0.74 and 0.58 respectively. Thus, this new rapid method based on determination of bacterial ATP monitored by luciferin-luciferase bioluminescence reaction can be used for the indirect counting of bacteria, respectively.

흰쥐에서 할로페리돌에 의해서 유발된 강경증에 미치는 후구 적출의 영향

전승호,김선장,배기환,이순철 충남대학교 약학대학 의약품개발연구소 1992 藥學論文集 Vol.8 No.-

We attempted to clarify the effect of bilateral olfactory bulbectomy on catalepsy induced by haloperidol in rats. The incidence of catalepsy induced by haloperidol remarkably increased after lesion of olfactory bulb, which was significantly inhibited by L-5-hydroxytryptophan, L-DOPA, and ginseng's total saponin but reserpine and α-methyl-p-tyrosine were ineffective. The dopamine content of brain was significantly decreased by olfactory bulbectomy, but this result was reversed by ginseng's total saponin.

hCG에 대한 단일클론 항체 생산과 그 이용에 관한 연구

崔尙勳,李丙哲,李承培 건국대학교 1987 論文集 Vol.25 No.1

This study was carried out to produce monoclonal antibody against hCG secreted by the tr-ophoblast of developing embryo. Produced monoclonal antibody to hCG was regarded as IgG class since it had a strong affinity for a protein A. The concentration of monoclonal antibody in cell culture supernatant and in mouse ascitic fluid was 250㎍ and 30mg perml, respectively. The titer of antibody in mouse ascitic fluid was about 1 : 106~107 on ELISA

The use of heparin chemistry to improve dental osteogenesis associated with implants

Lee, Sang Jin,Bae, Min Soo,Lee, Deok Won,Heo, Dong Nyoung,Lee, Donghyun,Heo, Min,Hong, Seoung-Jin,Kim, Joohyoung,Kim, Wan Doo,Park, Su A,Kwon, Il Keun Elsevier 2017 Carbohydrate polymers Vol.157 No.-

<P><B>Abstract</B></P> <P>In this study, we designed a hybrid Ti by heparin modifying the Ti surface followed by Growth/differentiation factor-5 (GDF-5) loading. After that, products were characterized by physicochemical analysis. Quantitative analysis of functionalized groups was also confirmed. The release behavior of GDF-5 grafted samples was confirmed for up to 21days. The surface modification process was found to be successful and to effectively immobilize GDF-5 and provide for its sustained release behavior. As an <I>in vitro</I> test, GDF-5 loaded Ti showed significantly enhanced osteogenic differentiation with increased calcium deposition under nontoxic conditions against periodontal ligament stem cells (PDLSc). Furthermore, an <I>in vivo</I> result showed that GDF-5 loaded Ti had a significant influence on new bone formation in a rabbit model. These results clearly confirmed that our strategy may suggest a useful paradigm by inducing osseo-integration as a means to remodeling and healing of bone defects for restorative procedures in dentistry.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Titanium surface was developed to selectively graft the polymer using bioactive chemistry. </LI> <LI> The developed hybrid titanium disc showed outstanding <I>in vitro</I> osteo-integration activities. </LI> <LI> The surface modified titanium implant exhibited significant bone tissue regeneration in a rabbit model. </LI> </UL> </P>

데이터베이스 산업 육성을 위한 법제화 연구

배성훈(Seoung Hun Bae),이명수(Myung-Soo Lee),서일교(Il-Gyo Seo),김선영(Sun-Young Kim),이재진(Jae-Jin Lee),김성철(Sung-Cheol Kim),임정선(Jung Sun Lim),신민수(Minsoo Shin) 강원대학교 비교법학연구소 2014 江原法學 Vol.43 No.-

최근 데이터베이스 산업의 중심이 데이터베이스에서 데이터로, 활용으로 확대되면서 데이터베이스 산업은 지식정보사회의 핵심 인프라이자 데이터 기반 경제의 고부가가치 산업으로 부상하고 있다. 그러나 중소기업 위주의 낮은 경쟁력, 품질관리 체계 미흡, 전문 인력 부족 등의 고질적인 문제를 안고 있으며, 이를 극복하기 위해서는 관련 법적 근거 마련이 필수적이다. 본 논문에서는 데이터베이스 산업 현황에 대해 고찰하고 관련 법률 제정의 필요성과 방향을 제시하여 국내 데이터베이스 산업의 새로운 도약의 기회를 소개하고 향후 데이터 기반 경제에 대비하고자 한다. Recently, the focus of database industry is moving from database to data itself with applications. Database industry is being emerged as an essential infrastructure of knowledge-based economy and becomes a new high value-added business. To become a new engine of national economic growth, database industry may have the potential problems such as relatively low competitiveness of relevant SMEs, lack of database quality management system, and shortage of professional manpower. One of ways to deal with these problems is to establish a legal basis. In this study, we have studied the current status of database industry. Based on the analysis, we have suggested the need for establishment of relevant law and the direction on how such law can be enacted. This study contributes to provide a new chance for the growth of database industry and build up momentum in order to make a step toward data based economy.

Environmental Assessment of Water, Sediment and Plants in the Mankyeong River, ROK

Deog-Bae Lee(이덕배),Kyeong-Bo Lee(이경보),Chang-Hwan Kim(김창환),Jong-Gu Kim(김종구),Chan-Won Park(박찬원),Seoung-Yong Na(나승용) 한국토양비료학회 2003 한국토양비료학회 학술발표회 초록집 Vol.2003 No.5