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Enzyme free cell detachment using pH-responsive poly(amino ester) for tissue regeneration
Tae-Jin Lee,Tepeng Wu,Jung Hwan Park,Jihun Song,Gun-Jae Jeong,Jiyu Hyun,Jooyoung Lee,Soo-Hong Lee,Doo Sung Lee,Suk Ho Bhang 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.88 No.-
Despite the advantages of stem cell injection to the skin wound site, this technique is limited in terms ofthe delivery of therapeutically functional stem cells to the wound site. Considering the abnormalmicroenvironment of a wound site, even a small damage or loss of cell function induced by severe trypsintreatment might lead to decrement of the therapeutic efficacy of stem cells. In this study, we havesynthesized pH-responsive polymer mixed poly(amino ester) (mPAE), which can provide the appropriatecell culture condition for cell growth without cytotoxicity along with the maintenance of extracellularmatrix after detachment using low pH (6.0) PBS. Human adipose derive stem cells (hADSCs) cultured onand detached from mPAE-coated tissue culture plates (TCPs) showed similar cell adhesion andangiogenic paracrine factor secretion, compared to hADSCs cultured on and detached from TCPs withtrypsin. As a result, hADSCs detached with pH 6.0 PBS from mPAE-coated TCPs showed similartherapeutic angiogenesis and wound regeneration, compared to the hADSCs detached with trypsin fromTCPs. In conclusion, our pH-responsive polymer, mPAE, might be utilized as an enzyme-free celldetachment system for future tissue engineering.
Regulation of DU145 prostate cancer cell growth by Scm-like with four mbt domains 2.
Lee, Kwanghyun,Na, Wonho,Maeng, Je-Heon,Wu, Hongjin,Ju, Bong-Gun Indian Academy of Sciences 2013 Journal of biosciences Vol.38 No.1
<P>Mammalian SFMBTs have been considered to be polycomb group repressors. However, molecular mechanisms underlying mammalian SFMBTs-mediated gene regulation and their biological function have not been characterized. In the present study, we identified YY1 and methylated histones as interacting proteins of human SFMBT2. We also found that human SFMBT2 binds preferentially to methylated histone H3 and H4 that are associated with transcriptional repression. Using DU145 prostate cancer cells as a model, we showed that SFMBT2 has a transcriptional repression activity on HOXB13 gene expression. In addition, occupancy of SFMBT2 coincided with enrichment of diand tri-methylated H3K9 and H4K20 as well as tri-methylated H3K27 at the HOXB13 gene promoter. When SFMBT2 was depleted by siRNA in DU145 prostate cancer cells, significant up-regulation of HOXB13 gene expression and decreased cell growth were observed. Collectively, our findings indicate that human SFMBT2 may regulate cell growth via epigenetic regulation of HOXB13 gene expression in DU145 prostate cancer cells.</P>
RCEPD With Enhanced Light Absorption by Crown-Shaped Quantum Well
Gun Wu Ju,Byung Hoon Na,Hee Ju Choi,Kwang Wook Park,Young Min Song,Yong Tak Lee Institute of Electrical and Electronics Engineers 2015 IEEE photonics technology letters Vol.27 No.19
<P>A high-performance resonant cavity enhanced photodetector (RCEPD) is developed by introducing an InGaAs/GaAs crown-shaped quantum well (CSQW) structure. In calculation, the absorption coefficient of the proposed CSQW structure is significantly enhanced by 47.8% compared with that of the conventional QW without increasing the electric field due to the large overlap of electron/hole-wave functions. To verify the feasibility of our proposed QW structure, we fabricate RCEPDs with a designed CSQW and the conventional QW structure. The fabricated CSQW-RCEPD exhibits a maximum quantum efficiency of 45.4%, an improvement of 36.2% in comparison with the conventional RCEPD. Moreover, the spectral bandwidth is 4.7 nm in the CSQW-RCEPD, which is in good agreement with the calculated result. The RCEPD with enhanced light absorption using a CSQW structure is highly promising for optical interconnect and sensing applications.</P>
Lee, Kwanghyun,Na, Wonho,Lee, Jee Youn,Na, Jungtae,Cho, Heejung,Wu, Hongjin,Yune, Tae Young,Kim, Won‐,Sun,Ju, Bong‐,Gun Blackwell Publishing Ltd 2012 Journal of Neurochemistry Vol.122 No.2
<P><I>J. Neurochem.</I> (2012) <B>122</B>, 272–282.</P><P><B>Abstract</B></P><P>The inflammatory response contributes substantially to secondary injury cascades after spinal cord injury, with both neurotoxic and protective effects. However, epigenetic regulations of inflammatory genes following spinal cord injury have yet to be characterized thoroughly. In this study, we found that histone H3K27me3 demethylase Jmjd3 expression is acutely up‐regulated in blood vessels of the injured spinal cord. We also observed up‐regulation of <I>Jmjd3</I> gene expression in bEnd.3 endothelial cells that were subjected to oxygen‐glucose deprivation/reperfusion injury. When <I>Jmjd3</I> was depleted by siRNA, oxygen‐glucose deprivation/reperfusion injury‐induced up‐regulation of IL‐6 was significantly inhibited. In addition, Jmjd3 associated with NF‐κB (p65/p50) and CCAAT‐enhancer‐binding protein β at the <I>IL‐6</I> gene promoter. The recruitment of Jmjd3 coincided with decreased levels of tri‐methylated H3K27 as well as increased levels of mono‐methylated H3K27 at the <I>IL‐6</I> gene promoter. Furthermore, <I>Jmjd3</I> depletion did not result in significant changes of methylation level of H3K27 at the <I>IL‐6</I> gene promoter. Collectively, our findings imply that Jmjd3‐mediated H3K27me3 demethylation is crucial for <I>IL‐6</I> gene activation in endothelial cells, and this molecular event may regulate acute inflammatory response and integrity of the blood‐spinal cord barrier following spinal cord injury.</P>
Coupled Inductor Incorporated Boost Half-Bridge Converter With Wide ZVS Operation Range
Sung-Sae Lee,Seung-Wu Rhee,Gun-Woo Moon IEEE 2009 IEEE transactions on industrial electronics Vol.56 No.7
<P>A new boost half-bridge (BHB) converter is presented. It is composed of an additional diode and a coupled winding to the boost inductor of the BHB converter. Using the transferring of a boost inductor current to the coupled winding, the cancellation of zero-voltage-switching (ZVS) current, which always occurs in a conventional one, is prevented. Therefore, the ZVS operation is easily achieved by the leakage inductor current of the transformer. Furthermore, since the negatively built-up leakage inductor current of the boost winding helps the ZVS operation throughout a wide load range, the ZVS operation is always guaranteed.</P>
Regorafenib prevents the development of emphysema in a murine elastase model
Kwangseok Oh,Gun-Wu Lee,Han-Byeol Kim,Jin Hee Park,Eun-YoungShin,Eung-GookKim 생화학분자생물학회 2023 BMB Reports Vol.56 No.8
Emphysema is a chronic obstructive lung disease characterized by inflammation and enlargement of the air spaces. Regorafenib, a potential senomorphic drug, exhibited a therapeutic effect in porcine pancreatic elastase (PPE)-induced emphysema in mice. In the current study we examined the preventive role of regorafenib in development of emphysema. Lung function tests and morphometry showed that oral administration of regorafenib (5 mg/kg/day) for seven days after instillation of PPE resulted in attenuation of emphysema. Mechanistically, regorafenib reduced the recruitment of inflammatory cells, particularly macrophages and neutrophils, in bronchoalveolar lavage fluid. In agreement with these findings, measurements using a cytokine array and ELISA showed that expression of inflammatory mediators including interleukin (IL)-1β, IL-6, and CXCL1/KC, and tissue inhibitor of matrix metalloprotease-1 (TIMP-1), was downregulated. The results of immunohistochemical analysis confirmed that expression of IL-6, CXCL1/KC, and TIMP-1 was reduced in the lung parenchyma. Collectively, the results support the preventive role of regorafenib in development of emphysema in mice and provide mechanistic insights into prevention strategies.