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A. LOISELLE-LAPOINTE,A. J. CONDE,H. RIBBERINK 한국자동차공학회 2017 International journal of automotive technology Vol.18 No.1
Environment Canada (EC) and Natural Resources Canada (NRCan) separately tested two 2012 Chevrolet Volts between 2013 and 2014 in Ottawa, Ontario on public roads in the summer and winter months using realistic cabin-climate control settings. More than 1300 trips were conducted over nine routes: three city, one congested, two arterial, one highway and two expressway routes. EC tests recorded cabin conditioning, traction battery and 12 V accessory power, select vehicle component temperatures, regulated emission rates and exhaust flow, and DC charge energy. Both NRCan and EC tests measured cumulative electrically driven distance (all-electric range), select CANbus signals and AC grid supply charge energy. Results from these studies were analysed to evaluate the overall performance of the Chevrolet Volt on public roads in climates representative of most of Canada (−27 °C to 37 °C) using realistic accessory settings. At 25 °C the Chevrolet Volt’s on-road all-electric EPA-method adjusted range is generally less than the U.S. EPA sticker rating (57.9 km). Cabin conditioning energy was found to be directly related to the difference between ambient and cabin temperature, except at low temperatures (< 0 °C) when the 1.4 L engine activates to assist the thermal management system. On average, heating the cabin in the winter months consumed significantly more electric energy than cooling the cabin in the summer months. Summer city and highway driving resulted in the lowest energy consumption (Wh/km), while congested and expressway driving cycles resulted in the highest. In the winter months, many differences between the drive cycles were not discernible due to the high cabin conditioning energy consumptions.
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강혜지,Gisèle LaPointe 한국미생물·생명공학회 2018 한국미생물·생명공학회지 Vol.46 No.2
The aim of this study was to transfer the 18.5 kb gene clusters coding for 17 genes from Lactobacillus rhamnosus to Lactococcus lactis subsp. cremoris MG1363 in order to determine the effect of host on exopolysaccharide (EPS) production and to provide a model for studying the phosphorylation of proteins which are proposed to be involved in EPS polymerization. Lactobacillus rhamnosus RW-9595M and ATCC 9595 have 99% identical operons coding for EPS biosynthesis, produced different amounts of EPS (543 vs 108 mg/l). L. lactis subsp. cremoris MG1363 transformed with the operons from RW-9595M and ATCC 9595 respectively, produced 326 and 302 mg/l EPS in M17 containing 0.5% glucose. The tyrosine protein kinase transmembrane modulator (Wzd) was proposed to participate in regulating chain elongation of EPS polymers by interacting with the tyrosine protein kinase Wze. While Wzd was found in phosphorylated form in the presence of the phosphorylated kinase (Wze), no phosphorylated proteins were detected when all nine tyrosines of Wzd were mutated to phenylalanine. Lactococcus lactis subsp. cremoris could produce higher amounts of EPS than other EPS-producing lactococci when expressing genes from L. rhamnosus. Phosphorylated Wzd was essential for the phosphorylation of Wze when expressed in vivo.
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Kang, Hye-Ji,LaPointe, Gisele The Korean Society for Microbiology and Biotechnol 2018 한국미생물·생명공학회지 Vol.46 No.2
The aim of this study was to transfer the 18.5 kb gene clusters coding for 17 genes from Lactobacillus rhamnosus to Lactococcus lactis subsp. cremoris MG1363 in order to determine the effect of host on exopolysaccharide (EPS) production and to provide a model for studying the phosphorylation of proteins which are proposed to be involved in EPS polymerization. Lactobacillus rhamnosus RW-9595M and ATCC 9595 have 99% identical operons coding for EPS biosynthesis, produced different amounts of EPS (543 vs 108 mg/l). L. lactis subsp. cremoris MG1363 transformed with the operons from RW-9595M and ATCC 9595 respectively, produced 326 and 302 mg/l EPS in M17 containing 0.5% glucose. The tyrosine protein kinase transmembrane modulator (Wzd) was proposed to participate in regulating chain elongation of EPS polymers by interacting with the tyrosine protein kinase Wze. While Wzd was found in phosphorylated form in the presence of the phosphorylated kinase (Wze), no phosphorylated proteins were detected when all nine tyrosines of Wzd were mutated to phenylalanine. Lactococcus lactis subsp. cremoris could produce higher amounts of EPS than other EPS-producing lactococci when expressing genes from L. rhamnosus. Phosphorylated Wzd was essential for the phosphorylation of Wze when expressed in vivo.
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( Hye-ji Kang ),( Gisele Lapointe ) 한국미생물생명공학회 2018 한국미생물·생명공학회지 Vol.46 No.2
The aim of this study was to transfer the 18.5 kb gene clusters coding for 17 genes from Lactobacillus rhamnosus to Lactococcus lactis subsp. cremoris MG1363 in order to determine the effect of host on exopolysac-charide (EPS) production and to provide a model for studying the phosphorylation of proteins which are proposed to be involved in EPS polymerization. Lactobacillus rhamnosus RW-9595M and ATCC 9595 have 99% identical operons coding for EPS biosynthesis, produced different amounts of EPS (543 vs 108 mg/l). L. lactis subsp. cremoris MG1363 transformed with the operons from RW-9595M and ATCC 9595 respectively, produced 326 and 302 mg/l EPS in M17 containing 0.5% glucose. The tyrosine protein kinase transmembrane modulator (Wzd) was proposed to participate in regulating chain elongation of EPS polymers by interacting with the tyrosine protein kinase Wze. While Wzd was found in phosphorylated form in the presence of the phosphorylated kinase (Wze), no phosphorylated proteins were detected when all nine tyrosines of Wzd were mutated to phenylalanine. Lactococcus lactis subsp. cremoris could produce higher amounts of EPS than other EPS-producing lactococci when expressing genes from L. rhamnosus. Phosphorylated Wzd was essential for the phosphorylation of Wze when expressed in vivo.
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Collin Yves,Hu Tina,Denault André,Fortier Annik,Beaubien-Souligny William,Lapointe Réal,Vandenbroucke-Menu Franck 대한마취통증의학회 2022 Korean Journal of Anesthesiology Vol.75 No.5
Background: Cerebral oximetry using near-infrared spectroscopy (NIRS) is used for monitoring cerebral oxygen saturation during cardiac surgery and is correlated with clinical outcomes. Our goal was to explore cerebral and somatic NIRS in liver resections as a predictor of post-operative complications. Methods: Prospective observational and non-interventional study from a tertiary care university hospital including adult patients undergoing liver resection monitored using NIRS at four sites before and during surgery. Those sites were: frontotemporal left and right zones, right thigh, and right arm. Anesthesiologists and surgeons were blinded to oximetry values. Correlations were assessed between baseline oximetry values and cerebro-somatic desaturation load (threshold of 80% from baseline) values with peri-operative events and complications. Results: Ninety patients were distributed equally among gender with a mean age of 59.7 ± 13.1 years. Lower baseline cerebral and/or somatic values were associated with increased risk of delirium, respiratory failure, surgical and renal complications, blood transfusions, and length of stay in the intensive care unit and in the hospital (P < 0.05). The severity of somatic desaturation below 80% was the only parameter associated with blood losses (P = 0.030) and length of hospital stay (P = 0.047). Conclusions: Cerebral and somatic desaturation does occur in liver resection and can be used simultaneously during liver surgery. Both baseline cerebral and somatic NIRS values are correlated with complications and outcomes. However, thigh desaturation appears more sensitive than cerebral NIRS values in predicting some of these complications.
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James F. Harwood,Jodi M. Fiorenzano,Elizabeth Gerardo,Theodore Black,Dennis A. LaPointe,Jeomhee Hasty 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.4
The Asian bush mosquito, Aedes japonicus japonicus (Theobald) was not known to occur in the Hawaii archipelago until it was identified on the island of Hawaii in 2003. This mosquito species remained undetected on the neighboring islands for 8 years before it was discovered at the Honolulu International Airport on Oahu in 2012. By 2015, four Ae. j. japonicus mosquitoes were collected in the western mountains of Oahu and one was collected in the central mountains of Kauai. The collection of this invasive mosquito species across the neighboring Hawaiian Islands of Oahu and Kauai indicated the need for increased seasonal surveillance on these islands. Following nearly four years of surveillance, Ae. j. japonicus was also confirmed to occur in the eastern mountains of Oahu and in the central mountainous region of Kauai. To expand the knowledge of the spread of invasive mosquitoes species further surveillance is necessary to identify all possible areas where populations of Ae. j. japonicus and other invasive mosquito species occur in Hawaiian archipelago.
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Reaction Time Dilemma in Parkinson Disease: Influence of Initiation Delay
In-sop Kim,Adrienne B. Hancock,Leonard L. LaPointe 한국언어치료학회 2010 言語治療硏究 Vol.19 No.4
Computerized testing provides precise performance measures, such as response time (RT) in milliseconds, and is widely used for non-invasive, cognitive assessment of neurologically impaired groups. Particularly when using a computerized measure to collect response time and accuracy data, assessments of speech, language, and cognitive function must consider the possible influence of motoric deficits. For example, tremor, rigidity, bradykinesia, and initiation delays may inflate the motoric response portion, resulting in a skewed total RT. This can be particularly confounding when attempting to determine if delayed responses on cognitive measures are the result of depressed cognition or altered motoric integrity. One approach to identifying the motoric contribution to RT has been to subtract the average RT on a simple response time (SRT) test from the RT on any other test (e.g., choice reaction time). However, this assumes that the motoric contribution to RT will remain constant across tests for each individual, and that all the processes involved in SRT are invariant across other tests. Though these corrections have attempted to deal with this “cognitive vs. motoric” dilemma, the nature and degree of motoric influence in RT remains unclear, particularly in movement disorders that are characterized by motoric initiation difficulty. It is conceivable that delay in RT may not by a sign of reduced cognitive processing, but merely the result of motoric initiation delay. This is the crux of the motoric-cognitive dilemma. In an attempt to address this issue and provide some insight into the performance of healthy elderly adults and Parkinson's disease patients, we investigated the contribution of initiation delay in computerized RT measures by comparing conditions with and without opportunity for initiation delay (interrupted -total 30sec, 5 sec per block, continuous-total 30sec per block). There were no significant differences between groups and between interrupted and continuous test conditions. These results suggest that the RT performance was not affected by significant delay in initiating a motoric hand response for these participants. These results may be indicative of expected motoric initiation performance in elderly adults and elderly adults with Parkinson's disease. The results of this study do not resolve the cognitive-motoric dilemma in PD reaction time assessment, but do contribute to the influence (or lack of influence) of motoric initiation issues in at least one sample of people with PD.
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Brownian dynamics of colloidal microspheres with tunable elastic properties from soft to hard
Yoon, Jiwon,Cardinaux, Fré,dé,ric,Lapointe, Clayton,Zhang, Chi,Mason, Thomas G.,Ahn, Kyung Hyun,Scheffold, Frank Elsevier 2018 Colloids and surfaces. A, Physicochemical and engi Vol.546 No.-
<P><B>Abstract</B></P> <P>We study the Brownian thermal motion of a colloidal model system made by emulsifying hot liquid <I>α</I>-eicosene wax into an aqueous surfactant solution of sodium dodecyl sulfate (SDS). When this waxy oil-in-water emulsion is cooled below <I>α</I>-eicosene's melting point of <I>T</I> <SUB> <I>c</I> </SUB> ≃ 25 °C, the microscale emulsion droplets solidify, effectively yielding a dispersed particulate system. So, the interiors of these wax droplets can be tuned from a viscous liquid to an elastic solid through very modest changes in absolute temperature. Using the multiple light scattering technique of diffusing wave spectroscopy (DWS), which is very sensitive to small-scale motion and shape fluctuations of dispersed colloidal objects, we show that the thermal fluctuations of the interfaces of these liquid droplets at higher temperature, seen in the DWS intensity–intensity correlation function at early times, effectively disappear when these droplets solidify at lower temperature. Thus, we show that the early-time behavior of this DWS correlation function can be used to probe mechanical properties of viscoelastic soft materials dispersed as droplets.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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