A reinforced suture method for stapled gastrointestinal anastomosis to reduce gastrointestinal hemorrhage during Whipple operation in laparoscopy

La Zhang,Liujun Jiang,Ning Jiang,Rui Liao,Lei Xiang,Baoyong Zhou,Dewei Li 대한외과학회 2022 Annals of Surgical Treatment and Research(ASRT) Vol.102 No.2

Purpose: Laparoscopy is being increasingly accepted for pancreaticoduodenectomy. Stapled anastomosis (SA) is used extensively to facilitate laparoscopic pancreaticoduodenectomy (LPD); however, the incidence of anastomotic bleeding after stapled gastrointestinal anastomosis is still high. Methods: One hundred and thirty-nine patients who underwent LPD using Whipple method were enrolled in our study. We performed the SA with our reinforced method (n = 68, R method) and without the method (n = 71, NR method). We compared the clinical characteristics and anastomosis methods of patients with or without gastrointestinal-anastomotic hemorrhage (GAH), and operative parameters were also compared between the anastomotic methods. Results: Of the 139 patients undergoing LPD, 15 of them developed GAH. The clinical characteristics of patients with or without GAH were not significantly different except in the anastomotic method (P < 0.001). In the univariate logistic regression analyses, only the anastomotic method was associated with GAH. Furthermore, patients with the NR method had significantly higher incidences of GAH (P < 0.001) and Clavien-Dindo grade ≥ III complications (P < 0.001). Conclusion: Our retrospective analysis showed that the SA performed with reinforced method might be a reform of SA without the reinforcement, as indicated by the lower incidence of GAH. However, further research is necessary to evaluate the utility of this reinforced method.

Intradiscal injection of simvastatin retards progression of intervertebral disc degeneration induced by stab injury

Zhang, Huina,Wang, Lin,Park, Jun Beom,Park, Paul,Yang, Victor C,Hollister, Scott J,La Marca, Frank,Lin, Chia-Ying BioMed Central 2009 Arthritis research & therapy Vol.11 No.6

<P><B>Introduction</B></P><P>Earlier work indicates that the cholesterol-lowering drug, simvastatin, is anabolic to chondrogenic expression of rat intervertebral disc (IVD) cells, which suggests a potential role for simvastatin in IVD regeneration. In this study, we expand on our earlier work to test the effectiveness of simvastatin on disc degeneration utilizing a rat tail disc degeneration model.</P><P><B>Methods</B></P><P>30 rats that underwent 21 G needle-puncture at rat tail discs were injected with simvastatin-loaded poly(ethylene glycol)-poly(lactic acid-co-glycolic acid)-poly(ethylene glycol) (PEG-PLGA-PEG) gel (5 mg/ml) or vehicle control at 4 weeks after needle injury. All animals were sacrificed 2 weeks after simvastatin injection. Bone morphogenetic protein-2 (BMP-2), aggrecan, collagen type II, and collagen type I messenger ribonucleic acid (mRNA) expression in the rat nucleus pulposus (NP) were measured by real-time polymerase chain reaction (PCR). <I>In vivo </I>magnetic resonance imaging (MRI) was performed to monitor changes in disc degeneration. Rat discs were also assessed by histology using hematoxylin and eosin (H&E) and safranin O staining. In addition, the NP weight, glycosaminoglycan (sGAG) and DNA content were also measured.</P><P><B>Results</B></P><P>A single dose of simvastatin loaded in thermo-sensitive PEG-PLGA-PEG gel injected into the NP had the trend to increase aggrecan expression and sGAG content, and significantly increased mRNA levels of BMP-2, collagen type II, and the differentiation index (the ratio of collagen type II to collagen type I). The decreased NP weight, T2 intensity, as well as MRI index in the rat tail discs induced by needle puncture were significantly reversed after 2 weeks of simvastatin treatment. In addition, simvastatin treatment also improved histological changes induced by needle puncture.</P><P><B>Conclusions</B></P><P>A single injection of simvastatin loaded in PEG-PLGA-PEG gel into rat tail discs had the potential to retard or regenerate the degenerative disc.</P>

Salicylic acid improves drought-stress tolerance by regulating the redox status and proline metabolism in Brassica rapa

Van Hien La,Bok-Rye Lee,Qian Zhang,Sang Hyun Park,Md. Tabibul Islam,Tae-HwanKim 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.1

Salicylic acid (SA) has been regarded as the important phytohormone in improving abiotic stress tolerance. However, thephysiological mechanisms on drought stress tolerance remain largely unknown. To investigate the role of SA in drought-stresstolerance in Chinese cabbage ( Brassica rapa subsp. pekinensis ) with regard to cellular redox control and proline metabolism,Chinese cabbages were pretreated or untreated with SA for 7 days, then grown under well-watered or drought-stressed conditionsfor 14 days. The osmotic potential and chlorophyll and carotenoid contents were decreased in the drought-stressedplants; however, the negative eff ects of drought on these parameters was substantially ameliorated in the SA-pretreatedplants. Drought stress resulted in increased O 2− , H 2 O 2 , and malondialdehyde (MDA) contents in the non-SA pretreatedplants relative to the SA-pretreated plants. Superoxide dismutase, catalase, guaiacol peroxidase, and ascorbate peroxidasewere highly activated in drought-stressed plants, whereas these substances were further activated in the SA-pretreated plantsunder drought stress. Drought stress resulted in a signifi cant decrease of the reduced/oxidized glutathione (GSH/GSSG) andreduced/oxidized nicotinamide adenine dinucleotide phosphate (NADPH/NADP) ratios, whereas plants pretreated with SAhad levels similar to those in the well-watered plants. Under drought conditions, SA pretreatment signifi cantly increasedproline content by up-regulating the expression of genes encoding pyrroline-5-carboxylate synthase ( P5CSA and P5CSB ) anddown-regulating the expression of the gene encoding proline dehydrogenase ( PDH ) compared to non-SA pretreated plants. These results indicate that a pretreatment with SA improves drought-stress tolerance by maintaining redox homeostasis andactivating proline biosynthesis.

Salicylic Acid Involves in Proline Synthesis and Redox Homeostasis Under Drought Stress in Brassica napus L.

H. V. La,B. R. Lee,Q. Zhang,T. M. Islam,S. H. Park,T. H. Kim 한국초지조사료학회 2016 한국초지조사료학회 학술대회논문집 Vol.2016 No.08

금형 높이가 다양한 프레스 공정의 금형 교체시간 단축 사례

정병호(Byung-Ho Jeong),라순영(Soon-Young La),박보은(Bo-Eun Park),장연상(Yan-Shuang Zhang) 한국산업경영시스템학회 2011 한국산업경영시스템학회지 Vol.34 No.2

The reduction of setup time is very important in a lot production system. A punch press is a typical system of lot production. This paper describes a case study to reduce setup time of a punch press manufacturing system. Especially, this case study reduced the time of die exchange in the case that the standardization of die height is impossible realistically because of diversity on die height and heavy expenses. This study adopts a supplementary die and supplementary blocks to make the use of auto-clamping device possible. The paper also gives a sequencing algorithm to reduce the number of exchange of the supplementary die.

An Aqueous Extract of Yunnan Baiyao Inhibits the Quorum-Sensing-Related Virulence of Pseudomonas aeruginosa

Zu-Guo Zhao,Shuang-Shuang Yan,Yun-Mei Yu,Na Mi,La-Xi Zhang,Jun Liu,Xiao-Ling Li,Fang Liu,Jun-Fa Xu,Wei-Qing Yang,Guo-Ming Li 한국미생물학회 2013 The journal of microbiology Vol.51 No.2

Yunnan Baiyao is a famous Chinese medicine that has long been directly applied to wounds to reduce bleeding, pain, and swelling without causing infection. However, little is known about its ability to prevent infection. The present study aimed to assess in vitro the anti-virulence activity of an aqueous extract of Yunnan Baiyao (YBX) using Pseudomonas aeruginosa as a pathogenic model. We found that a sub-MIC (2.5 mg/ml) of YBX can efficiently interfere with the quorum-sensing (QS) signaling circuit. Real-time polymerase chain reaction analysis showed that a sub-MIC of YBX downregulated the transcriptions of lasR, lasI, rhlR, and rhlI,which resulted in global attenuation of QS-regulated virulence activities, such as biofilm formation, and secretion of LasA protease, LasB elastase and pyocyanin. Further, YBX reduced the motility of P. aeruginosa related to QS, and impaired the formation of biofilms. These results suggest that YBX may possess global inhibitory activity against the virulence of P. aeruginosa and that YBX may also exhibit antimicrobial activity in vivo. The present study suggests that Yunnan Baiyao represents a potential source for isolating novel, safe, and efficacious antimicrobial agents.

Stabilizing breathing pattern using local mechanical vibrations: comparison of deterministic and stochastic stimulations in rodent models of apnea of prematurity

Dean Zeldich,Samer Bou Jawde,Jacob Herrmann,Leen Arnaout,Meghan Griffin,Noam Grunfeld,Yu Zhang,Ramaswamy Krishnan,Erzsébet Bartolák-Suki,Béla Suki 대한의용생체공학회 2021 Biomedical Engineering Letters (BMEL) Vol.11 No.4

Mechanical stimulation has been shown to reduce apnea of prematurity (AOP), a major concern in preterm infants. Previous work suggested that the underlying mechanism is stochastic resonance, amplification of a subthreshold signal by stochastic stimulation. We hypothesized that the mechanism behind the reduction of apnea length may not be a solely stochastic phenomenon, and suggest that a purely deterministic, non-random mechanical stimulation could be equally as effective. Mice and rats were anesthetized, tracheostomized, and mechanically ventilated to halt spontaneous breathing. Two miniature motors controlled by a microcontroller were attached around the abdomen. Ventilation was paused, stimulations were applied, and the time to the rodent’s first spontaneous breath (T) was measured. Six spectrally different signals were compared to one another and the no-stimulation control in mice. The most successful deterministic stimulation (D) at reducing apnea was then compared to a pseudo-random noise (PRN) signal of comparable amplitude and frequency. CO2%, CO2 stabilization time (Ts), O2 saturation (SpO2%), and T were also measured. D significantly reduced T compared to no stimulation for medium and high amplitudes. PRN also reduced T, without a difference between D and PRN. Furthermore, both stimulations significantly reduced Ts with no significant differences between the respective stimulations. However, there was no effect of D or PRN on SpO2%. The lack of differences between D and PRN led to an additional series of experiment comparing the same D to a band-limited white noise (WN) signal in young rats. Both D and WN were shown to significantly reduce T, with D showing statistical superiority in reduction of apnea. We further speculate that both deterministic and stochastic mechanical stimulations induce some form of mechanotransduction which is responsible for their efficacy, and our findings suggest that mechanical stimulation may be effective in treating AOP.