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새로운 LacZ Gene Fusion 및 Gene Cartridge Vector의 개발에 관한 연구
박교선,이영훈,박충웅,최창진 圓光大學校 基礎自然科學硏究所 1987 基礎科學硏究誌 Vol.6 No.2
In this study, a new lacZ gene vector, plasmid PGEM3-lacZ was constucted, which could be used for the study of the gene regulation and the function of proteins. Plasmid pMC1871 was digested with restriction endonuclease Pst I and Sma I to obtain a DNA fragment with the lacZ-gene(3091 bp). Recombinant plasmid pGEM3-lacZ was constructed by inserting the lacZ gene DNA(Pst I-Sma I cut DNA; 3091 bp) of pMC1871 into the Pst I and Hinc Ⅱ sites of plasmid pGEM3. After then, Recombinant plasmid pGEM3-lacZ DNA was transformated into E. coli HB 101. pGEM3-lacZ DNA/HB 101 was screened on the NZY plate with ampicillin and X-gal(5-Bromo-4-Chloro-3-Indolyl-β-D-Galactoside) by selecting blue colonies. The restriction map of pGEM.3-1acZ DNA was constructed by the following restriction enzymes :Bgl I, Xbal I, Bam HI, EcoR I, Hind Ⅲ, Kpn I, Pst I, Sma Ⅰ, Sal Ⅰ. From the restriction map, the lacZ DNA was confirmed to be present at the expected position in the plasmid pGEM3-lacZ. pGEM3-lacZ/HB101 gives about 10 times more lacZ DNA than pMC 1871/HB101.
박선영,조필교,이양심,윤인경,임양순,최창숙,장명희 한국 가정과 교육 학회 1998 한국가정과교육학회지 Vol.10 No.2
The purpose of the study is to develop a Performance Assessment Method & Tools for Home Economics at middle school. Through the content analyses of middle school Home Economics curriculum and 8 textbooks of lst, 2nd, and 3rd grade respectively, teching elements for each content area were drawn and each specific learning objectives were idntified accordingly. In addition, assessment element for student's performance in each teaching element were specified for analysis. Analytic framework for the development of Performance Assessment Method and Tools were proposed by each teaching element. Beased on the analytic framework proposed, a model method and tools were developed by 10 different test measures of description written test, statement written test, oral test, pro and con discussion, performance test, experiment, interview, observation, self-test report, and portfolio. The model proposed in the study has been modified and improved through the feasibility studies of experts' examination and field research application. The model assessment method and tools for the study has been compleeted finally by proposing 45 performance assessment tools for the content areas of food, clothing, housing, resource management and consumerism, human development and family relationship.
金暎來,朴敎善,朴順五,李永馥 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-
Leaf tissue segments of cabbage (Brassica oleracea. L.) were cultured on the MS medium supplemented with BA and 2, 4-D or NAA. 2, 4-D promoted callus induction but not shoot or root organogenesis. The highest callus induction frequency obtained at treatment of BA 0.2 and 2, 4-D 0.2 ㎎/l. High concentration of 2, 4-D 5.0㎎/l inhibited callus formation. BA promoted shoot formation at the 1.0㎎/ℓ and complementary treatment of BA and NAA was more effective on the formation of shoot and root. Multiple shoots were formed on the medium supplemented with BA 1.0 and NAA 0.5-1.0㎎/l, and BA 0.5 and NAA 2.0㎎/l. Higher concentration of NAA 5.0㎎/ℓ inhibited shoot formation. The highest root formation was induced at the NAA 1.0-2.0㎎/l. Embryogenesis was not induced on leaf disk culture.
전주시 도심부 주차장의 효율적인 관리·운영 방안에 관한 연구
유응교,박창선 全北大學校 附設 都市및環境硏究所 1994 都市 및 環境硏究 Vol.9 No.1
The purpose of this study is to examine the parking problem and policy in the CBD of Chonju City, and to identify its impremention plan for improving the parking situation there from a planning and institutional perspective The rapidly increase in car ownership and insufficient parking space have compounded the parking preblem of this are 1. A production of parking space present condition and parking guidebook 2. The engaging system for parking space situation 3. A proposition of the parking space zoning
Kyo Sun Park,Hae Keun Yun,Jeong Ho Rho,Youn Jung Choi,Hee Jae Lee 한국원예학회 2005 Horticulture, Environment, and Biotechnology Vol.46 No.2
Stenospermocarpic seedless grape cultivars have commonly been used for introducing seedlessness in breeding programs. For improving breeding efficiency for seedless grape cultivars, ovule culture was conducted to find the proper methods for germinating immature ovules and regenerating plantlets of stenospermocarpic seedless cultivars. The optimal harvesting and excising time of ovules from the berries of ‘Cheongsoo’, ‘Benizawa’, and ‘Himrod Seedless’ using the Nitch and Nitch (NN) culture media of NN-3, NN-2, and NN-3 were found to be 50, 50, and 40 days after full bloom, respectively. Under these conditions, the ovule germinations were 2.5, 10.0, and 25.0% for ‘Cheongsoo’, ‘Benizawa’, and ‘Himrod Seedless’, respectively. ‘Himrod Seedless’ was considered to be the best among the stenospermocarpic grape cultivars tested for in vitro ovule culture in the present study. Plantlets from the ovule culture could successfully be regenerated.
Organic Solvent and pH Induced Alteration of Product Specificity of CGTase
Park, Kyo-Sun,Oh, Hyun-Mi,Choe, Hui-Woog,Park, Chung-Ung,Lee, Kang-Min The Korean Society for Biotechnology and Bioengine 1998 Biotechnology and Bioprocess Engineering Vol.3 No.2
Cyclodextrin glucanotransferase [CGTase, E.C.2.4.1.19] is an extracellular enzyme, which catalyzes he formation of ${\alpha}$-, ${\beta}$-, ${\gamma}$- CDs from starch. Their proportions of formations depend on enzyme sources and reaction conditions. To understand what determines the product specificity of CGTases, we examined the alteration of product specificity of CGTase from Bacillus macerans by organic solvent sand pH. At acidic pH range less than pH 6 where the enzyme was unstable, the ratio of ${\alpha}$-/ ${\beta}$-CD production was increased 4 times more than that at neutral pH range. As we increased the concentration of 2-butanol, ${\alpha}$-/ ${\beta}$-CD ratio was proportionally increased but / ratio remained constant. The ${\alpha}$-/ ${\beta}$-CD ratio of products was increased in the reaction media which yielded low products.