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- 저자 : Kun Gao (검색결과 63 건)
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Gao Shan-shan,Li Dong-yu,Huo Zhuang-kun,Zhang Yong-lei,Cao Yi-zhuo,Tan Yue-yao,Guo Xin-long,Zhang Jia-hao,Zhang Kun-peng,Li Rui-min 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.4
Insect glutathione S-transferases (GSTs) play a crucial role in the detoxification of exogenous compounds, especially insecticides and plant allelochemicals. A sigma class GST gene, TcGSTS7, mediates the response to eugenol in Tribolium castaneum. However, the mechanism underlying this effect remains largely unknown. In this study, TcGSTS7, which exhibits a structural motif and domain organization characteristic of GSTs, was cloned from the T. castaneum genome. Spatiotemporal expression analysis revealed that TcGSTS7 was most highly expressed at the late larva stage and was mainly expressed in the fat body and epidermis of larvae and adults, suggesting that TcGSTS7 may play a potential role in the protection against toxic xenobiotics in T. castaneum. Furthermore, the expression of TcGSTS7 was significantly induced after exposure to eugenol, while RNA inter ference (RNAi) targeting TcGSTS7 enhanced the sensitivity of the beetle to eugenol, indicating that TcGSTS7 is involved in the tolerance of T. castaneum to this insecticide. Interestingly, the depletion of TcCncC, which encodes a transcription factor of the CncC pathway that has been associated with the regulation of detoxification-related genes in insects, led to a reduction in the TcGSTS7 transcript level following exposure to eugenol, which suggests that TcGSTS7 acts downstream of the CncC pathway. Combined, these results indicated that TcGSTS7 partici pates in the tolerance of T. castaneum to phytochemicals in a CncC pathway-dependent manner. These findings have implications for the development of novel drugs for use in pest control.
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Kun Gao,Xiang Yuan Deng,Meng Ke Shang,Heying Qian,Xijie Guo 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3
The cDNA of a biphenyl hydrolase-like (BPHL) protein from Bombyx mori was cloned via rapid amplification of cDNA ends and submitted to GenBank under accession number JN020647. The full-length BmBPHL cDNA was 1161 bp, with four exons and three introns. It consisted of a 208 bp 5′-terminal untranslated region (UTR) and a 191 bp 3′-UTR with three polyadenylation signal sequences AATAAA and a poly(A) tail. The BmBPHL cDNA encodes a 253–amino acid polypeptide with a theoretical isoelectric point of 8.67 and a predicted molecular weight of 28.9 kDa. The deduced amino acid sequence of BmBPHL contained an abhydrolase_6 domain and the Gly-X-Ser-X-Gly motif that is characteristic of serine hydrolases. Sequence comparison showed that BmBPHL is 51% identical to Tribolium castaneum BPHL and 50% identical to Nasonia vitripennis BPHL. Phylogenetic analysis revealed that BmBPHL is grouped with insect BPHL proteins, separating from vertebrate BPHLs. The BmBPHL mRNA transcripts were mainly detected in hemolymph and fat body using fluorescent quantitative real-time PCR. In addition, infectionwith B. mori cytoplasmic polyhedrosis virus (BmCPV) upregulated the relative BmBPHL expression in the hemolymph and midgut. Therefore, BmBPHL may have an important function in the response of silkworms to BmCPV infection.
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Gao, Jian-Kun,Wang, Li-Xia,Long, Bo,Ye, Xian-Tao,Su, Jing-Na,Yin, Xu-Yuan,Zhou, Xiu-Xia,Wang, Zhi-Wei Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9
Arsenic trioxide (ATO) has been found to exert anti-cancer activity in various human malignancies. However, the molecular mechanisms by which ATO inhibits tumorigenesis are not fully elucidated. In the current study, we explored the molecular basis of ATO-mediated tumor growth inhibition in pancreatic cancer cells. We used multiple approaches such as MTT assay, wound healing assay, Transwell invasion assay, annexin V-FITC, cell cycle analysis, RT-PCR and Western blotting to achieve our goal. We found that ATO treatment effectively caused cell growth inhibition, suppressed clonogenic potential and induced G2-M cell cycle arrest and apoptosis in pancreatic cancer cells. Moreover, we observed a significant down-regulation of Skp2 after treatment with ATO. Furthermore, we revealed that ATO regulated Skp2 downstream genes such as FOXO1 and p53. These findings demonstrate that inhibition of Skp2 could be a novel strategy for the treatment of pancreatic cancer by ATO.
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Jun-wei Gao,Zhi-hai Peng,Xiao-yu Li,Bo Sun,Yan-kun Guo,Gao-lin Liu 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.1
In this study, we determined the pharmacokinetics of mycophenolic acid (MPA) and its metabolites mycophenolic acid glucuronide (MPAG) and acyl glucuronide (AcMPAG) in rat plasma and bile, using a newly developed HPLC method. Protein precipitation and liquid-liquid extraction were employed in sample preparation of plasma and bile, respectively. The HPLC methods included a gradient elution consisting of acetonitrile and phosphate buffer at a flow rate of 1.2 mL/min, with UV detection at 254 nm. The HPLC method was found to be sensitive and linear (r^2 ≥ 0.9991, 1.0-128.0 and 0.25-32.0 mg/L for MPA; 1.0-128.0 and 0.5-64.0 mg/L for MPAG; 0.25-32.0 and 1.0-128.0 mg/L for AcMPAG in rat plasma and bile, respectively), precise (both the intra- and inter-day variability were ≤ 6.8%), and accurate (both the intra- and inter-day accuracy were between 92.2% and 105.4%). The average extraction efficiencies for MPA, MPAG and AcMPAG were 85.3%, 100.1%, and 94.7% in plasma, and 88.0%, 67.3%, and 68.3% in bile, respectively. The method was successfully employed for pharmacokinetic studies in plasma and bile after oral administration of mycophenolate mofetil (prodrug of MPA) in rats.
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( Kun Xu ),( Yuanyuan Gao ),( Xiaoxin Yi ),( Yajun Zhang ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.11
In this paper, diversity-multiplexing tradeoff (DMT) of three-user wireless multiple-antenna cooperative systems is investigated in general fading channels when half-duplex and decode-and-forward relay is employed. Three protocols, i.e., adaptive protocol, receive diversity protocol, and dual-hop relaying protocol, are considered. The general fading channels may include transmit and/or receive correlation and nonzero channel means, and are extensions of independent and identically distributed Rayleigh or Rician fading channels. Firstly, simple DMT expressions are derived for general fading channels with zero channel means and no correlation when users employ arbitrary number of antennas. Explicit DMT expressions are also obtained when all users employ the same number of antennas and the channels between any two users are of the same fading statistics. Finally, the impact of nonzero channel means and/or correlation on DMT is evaluated. It is revealed theoretically that the DMTs depend on the number of antennas at each user, channel means (except for Rayleigh and Rician fading statistics), transmit and/or receive correlation, and the polynomial behavior near zero of the channel gain probability density function. Examples are also provided to illustrate the analysis and results.
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Gao Shanshan,Zhang Xinyi,Zhou Can,Zhang Guangyan,Zhang Yong-lei,Li Rui-min,Zhang Kun-peng 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4
Insect odorant binding proteins (OBPs) paly an important role in the recognition and transmission signals. One odorant binding protein gene, TcasOBP7G, was significantly up-regulated after exposure to eucalyptol. Never theless, the causal role of TcasOBP7G in Tribolium castaneum is still unclear. Here, TcasOBP7G was cloned and characterized from T. castaneum, aligning with the typical characteristic of Minus-C OBPs. The analysis of gene expression profile showed that TcasOBP7G is highly expressed at late larval and adult stage, with particularly high expression levels in ovary and testis of adults, suggesting that TcasOBP7G might be associated with reproduction of T. castaneum. Furthermore, RNAi of TcasOBP7G led to a decrease in spawning, while the development of gonads was normal, suggesting that TcasOBP7G may medicate the reproduction of T. castaneum. Additionally, the mRNA level of TcasOBP7G was significantly increased after exposure to eucalyptol, and knockdown of TcasOBP7G resulted in an increased susceptibility of T. castaneum to eucalyptol, indicating that TcasOBP7G is associated with the tolerance of T. castaneum to eucalyptol. Altogether, these results indicate that TcasOBP7G is required for both reproduction and defense against phytochemical in T. castaneum. This study will provide a theoretical basis for the development of novel control strategies based on the olfaction of insects.
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IFree Filter-Based User Positioning for Satellite-Based Augmentation System
Kun Li,Xuanwen Wang,Shuaiyong Zheng,Shuailong Chen,Mengzhi Gao,Peng Yang,Xiaoqin Jin,Jianlei Yang 한국항공우주학회 2024 International Journal of Aeronautical and Space Sc Vol.25 No.2
Ionospheric storms and scintillation cause great impact on the service performance of single-frequency Satellite-Based Augmentation System (SBAS). On the one hand, conservative estimation method of the ionospheric integrity information can lead to the problem of over-wrapping in the pseudorange domain and cannot make it optimal to the service performance of SBAS. On the other hand, the performance of the ionospheric Kriging method is unstable in some regions with active ionospheric. Meanwhile, there will be a growing number of dual-frequency users with the development of GNSS. This paper proposes IFree Filter-based user positioning algorithm for SBAS and analyzes the positioning performance of 36 Monitor stations in North America. First, high-order differential method is used to detect cycle slip of dual-frequency carrier-phase observation, and chi-square test is used to detect and eliminate outliers. Then, the dual-frequency pseudorange is smoothed with the dual-frequency carrier phase after cycle slip repair, and the ionospheric delay is treated with IFree filter. Compared with the Wide Area Augmentation System (WAAS) algorithm, the signal-in-space correction accuracy by the proposed algorithm is significantly improved, which can provide LPV200 services for most regions in North America and even CAT-I services for some regions, and can be applied as key technologies to monitor stations and information processing center of the civil services platform for BeiDou Satellite-Based Augmentation System (BDSBAS).
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Gao Liai,Zhou Yongjie,Su Kun,Hou Chenwei,Huo Limin 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.10
It is one of the effective methods to improve the reliability of the distribution system by using looped network power supply. With the development of new energy technology, distributed generation has been more widely used. But a small power system named micro-grid which is assembled by the device such as distributed generation, energy storage device and controllable load in a certain operating rules catches the attention of people. It can operate in islanded mode with the power grid failure, which can greatly improve the reliability of the system. Traditional analytical method and Monte Carlo method are difficult to evaluate its reliability accurately. In this paper, a new artificial intelligence method based on Bayesian networks is used for the system reliability analysis and evaluation. The corresponding Bayesian networks are established for the reliability test bus 2 which containing looped network and the improve reliability test bus 6 which containing micro-grids separately. With the help of causal reasoning and diagnostic reasoning, the supply influence of different types of DG output intermittent, operation mode on loads are detected, the key factors which have an impact on important load power supply reliability are found out, which is the theoretical foundation for practical engineering decision, so as to improve system reliability, identify failures and make maintenance scheduling.
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