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Cho, Hyun-Kug,Kim, Hee-Hang,Seo, Dong-Ho,Jung, Jong-Hyun,Park, Ji-Hae,Baek, Nam-In,Kim, Myo-Jeong,Yoo, Sang-Ho,Cha, Jaeho,Kim, Young-Rok,Park, Cheon-Seok Elsevier 2011 Enzyme and microbial technology Vol.49 No.2
<P><B>Abstract</B></P><P>Amylosucrase (ASase, EC 2.4.1.4) is a glucosyltransferase that hydrolyzes sucrose into glucose and fructose and produces amylose-like glucan polymers from the released glucose. (+)-Catechin is a plant polyphenolic metabolite having skin-whitening and antioxidant activities. In this study, the ASase gene from <I>Deinococcus geothermalis</I> (<I>dgas</I>) was expressed in <I>Escherichia coli</I>, while the recombinant DGAS enzyme was purified using a glutathione S-transferase fusion system. The (+)-catechin glycoside derivatives were synthesized from (+)-catechin using DGAS transglycosylation activity. We confirmed the presence of two major transglycosylation products using TLC. The (+)-catechin transglycosylation products were isolated using silica gel open column chromatography and recycling-HPLC. Two (+)-catechin major transfer products were determined through <SUP>1</SUP>H and <SUP>13</SUP>C NMR to be (+)-catechin-3′-O-α-<SMALL>D</SMALL>-glucopyranoside with a glucose molecule linked to (+)-catechin and (+)-catechin-3′-O-α-D-maltoside with a maltose linked to (+)-catechin. The presence of (+)-catechin maltooligosaccharides in the DGAS reaction was also confirmed via recycling-HPLC and enzymatic analysis. The effects of various reaction conditions (temperature, enzyme concentration, and molar ratio of acceptor and donor) on the yield and type of (+)-catechin glycosides were investigated.</P>
Young-Kug Choo, Jae-Sung Ryu, Malg-Um Lim, Mi-Ran Hwang, Hyun-Ki Min, Gislain Moussavou, Ji-Su Kim, Sun-Uk Kim, Kisung Ko, Young-Ho Cho, Sang-Yoon Nam, Kyu-Tae Chang 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.4
Flavonoids have a range of biological activities, including anti-allergic, anti-inflammatory, anti-microbial, and anti-cancer activities, as demonstrated by in vitro studies. In this study, we investigated whether luteolin can be applied to suppression of lipopolysaccharide (LPS)-stimulated inflammatory responses in murine macrophages. Luteolin was found to reduce nitric oxide (NO) production in LPS-stimulated Raw 264.7 cells. In addition, expression of inducible nitric oxide synthetase (iNOS), cyclooxygenase-2 (COX-2), and pro-inflammatory cytokine tumor necrotic factor-α (TNF-α) at the mRNA and protein levels were decreased. These inhibitory effects were found to be caused by the blockade of nuclear factor kappa-light- chain-enhancer of activated B cells (NF-κB) activation and phosphorylation of mitogen-activated protein (MAP) kinase family, extracellular signal-regulated kinases 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), and p38 MAP kinase. In addition, pre-treatment with luteolin resulted in reduced ganglioside expression levels and inhibited expression of GT1b in Raw 264.7 cells. On the basis of these observations, we suggest that luteolin has potential as an anti-inflammatory drug candidate, and ganglioside GT1b may play a role in the inflammatory process.
UV Nanoimprint 공정에서 레지스트의 두께가 기포 생성에 미치는 영향에 대한 수치해석
김동현(Dong-Hyun Kim),김성용(Seong-Yong Kim),김국원(Kug Weon Kim),이우영(Woo Young Lee),김남웅(Nam Woong Kim) 한국산학기술학회 2014 한국산학기술학회 학술대회 Vol.- No.-
NIL 기술은 공정의 고속화 및 대면적화를 통한 대량생산 기술로의 전환하고 있다. UV-NIL의 경우 비 진공환경에서 공정이 가능하다면 설비비용을 낮추고 공정시간을 단축하는데 큰 기여를 할 수 있다. 그러나 이 경우 시급한 문제는 기포(Bubble)결함을 해결하는 것이다. 즉 비 진공환경에서는 공정 중 공기의 유입에 의해 패턴에 기포결함이 발생하게 되는데, 기포를 완전히 제거 혹은 억제하는 연구가 시급한 실정이다. 본 논문에서는 UV-NIL 공정에서의 기포결함에 대한 해석적 연구를 수행하였다. 유동해석 전문 프로그램인 ANSYS Fluent와 유체체적분율 방법인 VOF(Volume of Fluid)를 이용하였고, 몰드의 레지스트 두께 변화와 접촉각에 따른 기포형성 변화를 분석하였다.
Number of Stent Retriever Passes Associated With Futile Recanalization in Acute Stroke
Baek, Jang-Hyun,Kim, Byung Moon,Heo, Ji Hoe,Nam, Hyo Suk,Kim, Young Dae,Park, Hyungjong,Bang, Oh Young,Yoo, Joonsang,Kim, Dong Joon,Jeon, Pyoung,Baik, Seung Kug,Suh, Sang Hyun,Lee, Kyung-Yul,Kwak, Hyo Ovid Technologies Wolters Kluwer -American Heart A 2018 Stroke Vol.49 No.9
Choi, Kwang-Hyun,Lee, Kug-Seung,Jeon, Tae-Yeol,Park, Hee-Young,Jung, Nam-Gee,Chung, Young-Hoon,Sung, Yung-Eun The Korean Electrochemical Society 2010 Journal of electrochemical science and technology Vol.1 No.1
Alloying degree is an important structural factor of PtRu catalysts for direct methanol fuel cells (DMFC). In this work, carbon supported PtRu catalysts were synthesized by reduction method using anhydrous ethanol as a solvent and $NaBH_4$ as a reducing agent. Using anhydrous ethanol as a solvent resulted in high alloying degree and good dispersion. The morphological structure and crystallanity of synthesized catalysts were characterized by X-ray diffraction (XRD), high resolution transmission electron microscope (HR-TEM). CO stripping and methanol oxidation reaction were measured. Due to high alloying degree catalyst prepared in anhydrous ethanol, exhibited low onset potential for methanol oxidation and negative peak shift of CO oxidation than commercial sample. Consequently, samples, applying ethanol as a solvent, exhibited not only enhanced CO oxidation, but also increased methanol oxidation reaction (MOR) activity compared with commercial PtRu/C (40 wt%, E-tek) and 40 wt% PtRu/C prepared in water solution.