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Jinmoon Kim,장성일,Aeryun Kim,Hanfu Su,Niluka Gunawardhana,Yeong-Eui Jeon,Eun Jung Bak,김지혜,차정헌 한국미생물학회 2016 The journal of microbiology Vol.54 No.5
Mammalian γ-glutamyltranspeptidase (GGT) has been identified as a bone-resorbing factor. Since GGT of Bacillus subtilis exhibits similarity in their primary structure and enzymatic characteristics with mammalian GGTs, the bone-resorbing activity of bacterial GGT was examined in this study. Osteoclastogenesis was performed in a co-culture system of mouse calvaria-derived osteoblasts and bone marrow cells. A conditioned medium from GGT-overproducing B. subtilis culture showed significantly higher activity of osteoclast formation than a conditioned medium from wild-type B. subtilis culture. Recombinant GGT (rGGT) of wild-type B. subtilis and an enzymatic activity-defected rGGT of B. subtilis 2288 mutant were expressed in Escherichia coli and purified using His tag. Both purified rGGTs induced similar levels of osteoclastogenesis, suggesting that B. subtilis GGT possesses virulent boneresorbing activity and its activity is probably independent of its enzymatic activity. Furthermore, a recombinant protein of B. subtilis GGT heavy subunit (Bs rGGT/H) showed strong activity of osteoclastogenesis while the light subunit failed to show strong activity, suggesting that the bone-resorbing activity is mainly located at the heavy subunit. More importantly, the GGT enzymatic activity may not be required for this virulence activity since the light subunit contains the catalytic pocket. In addition, B. subtilis rGGT stimulated mRNA expressions of receptor activator of nuclear factor kappa-B ligand (RANKL) and cyclooxygenase-2 (COX-2), while an osteoprotegerin inhibited the osteoclast formation induced by Bs rGGT/H. This is the first demonstration that bacterial GGT itself is sufficient to act as a bone-resorbing virulence factor via RANKL-dependent pathway. Therefore, it can be hypothesized that GGT of periodontopathic bacteria may play an important role as a virulence factor in bone destruction.
Aeryun Kim,Stephanie L. Servetas,Jieun Kang,Jinmoon Kim,장성일,Yun Hui Choi,Hanfu Su,Yeong-Eui Jeon,Youngmin A. Hong,유윤정,D. Scott Merrell,차정헌 한국미생물학회 2016 The journal of microbiology Vol.54 No.12
The array of outer membrane proteins (OMPs) found in Helicobacter pylori provides a crucial component for persistent colonization within the gastric niche. Not only does H. pylori harbor a wide number of OMPs, but these OMPs often vary across strains; this likely contributes to immune evasion, adaptation during long term colonization, and potentially differential disease progression. Previous work from our group described OMP differences among the Bab family (babA, babB, and babC) and Hom family (homA and homB) from 80 American H. pylori clinical isolates (AH) and 80 South Korean H. pylori clinical isolates (KH). In the current study, we expanded our investigation to include the less well characterized Hom family member, HomC. Overall, we identified and genotyped three homC variants: homCS, homCL, and homCM, in both populations. Similar to other polymorphic genes, the KH group showed less overall diversity, with 97.5% of strains harboring homCL. In contrast, a more heterogeneous profile was observed in strains derived from an American population; we found nearly equal distribution of homCS and homCL. Further analysis of the AH group identified associations between homC polymorphism and bab genotype; in AH strains, there was a significant association between homCL and carriage of babA at locus A. Since babA is an important virulence factor for the development of severe gastric disease, these data may suggest that homC polymorphism plays a role in H. pylori pathogenesis.
토크 컨버터 록업클러치 마찰면 온도에 대한 수치해석적 연구
김희영(Heeyoung Kim),권의섭(Eaisub Gwon),김진문(Jinmoon Kim),장재덕(Jaeduk Jang),주인식(Insik Joo) 한국자동차공학회 2012 한국자동차공학회 학술대회 및 전시회 Vol.2012 No.11
Automatic transmission vehicle has many advantages over the convenience of driving, acceleration, smooth shifting and the small impact than manual transmission. But, Torque converter used ATF to transfer the power of an engine to an input shaft. Driveline system moved by working fluids brings the loss of power. To solve this problem, a key element of torque converter functions is a Lock-up clutch. The torque converter connects an engine and auto-transmission directly for better fuel enconomy. To reduce more fuel consumption, the slip is generated between a friction facing and front cover these days. The slip however has the bad influence on the torque converter. the heat caused by the slip make the deformation of torque converter. After all, the powertrain system is out of order. In this study we proposed the new analysis method. To analysis the heat transfer between the friction facing and a front cover, the contact pressure analysis is carrien out first. The heat generation predicted is used as the boundary condition of CFD. The comparison between numerical analysis data and experiment data has also been performed.
CFD를 활용한 토크컨버터 유동성능 해석의 최적기법 연구
김희연(Heeyeon Kim),권의섭(Eausyb Gwon),김진문(Jinmoon Kim),장재덕(Jaeduk Jang),주인식(Insik Joo) 한국자동차공학회 2013 한국자동차공학회 부문종합 학술대회 Vol.2013 No.5
Torque converter located in automatic transmission is important role that absorb the vibration of the engine and torque conversion. To improve vehicle performance, development of the torque converter matching to advanced engine performance is essential. The torque converter consist of impeller, turbine ,reactor and complex three dimensional shape. In order to implement a fluid performance according to the characteristics of the vehicle. Final shape is determined through repeated analysis. Flow analysis of the torque converter is essential. Time required for the analysis and accuracy of the analysis is most important. The paper proves that how to minimize the space and analysis method for determining the size of the grid to maintain the accuracy of the interpretation.
차량 경량화를 위한 토러스 축소와 고토크 토크컨버터 연구
김희연(Heeyeon Kim),김진문(Jinmoon Kim),장재덕(Jaeduk Jang),주인식(Insik Joo) 한국자동차공학회 2012 한국자동차공학회 부문종합 학술대회 Vol.2012 No.5
Torque converter in automatic transmission transfer the engine power to transmission by ATF. Lock-up clutch of torque converter in automatic transmission has been recently designed with a significant purpose of achieving a more fuel economy. The newly developed round-high torus for short axial length achieves a high torque capacity and high efficiency. The new elliptical torus torque converter replaces the full torus about Φ205mm some of the Φ220 ~ Φ230mm converter that were previously used in similar applications. In a fuel-consumption conscious world, the minimum goal for the new elliptical torus converter must be to perform at least as well as its predecessors. To reduce development time and cost, many kinds of CAE technology is used. This paper describes for the new torus torque converter with lock-up operation.
최윤희,Lai Jing,Kim Myeong-A,Kim Aeryun,Kim Jinmoon,Su Hanfu,Ge Linhu,Cha Jeong-Heon 한국미생물학회 2021 The journal of microbiology Vol.59 No.8
Helicobacter pylori colonizes human gastric mucosa. Its infection is associated with gastric diseases including gastric cancer. CagA is one of the most important toxins produced by H. pylori. It is related to gastric cancer which can be injected into host cells via a type IV secretion system (T4SS). CagL is a structural component of T4SS apparatus, which triggers host cell signaling pathway. It has been reported that CagL polymorphisms may influence the severity of disease development. To explore the contribution of CagL polymorphisms between East Asian and Western H. pylori in pathogenesis, cagL gene in G27 H. pylori was swapped by K74 cagL which is identical to East Asian CagL consensus sequence and by Western 26695 H. pylori, resulting in G27ΔcagL/cagLK74 and G27ΔcagL/cagL26695, respectively. Intriguingly, G27ΔcagL/ cagLK74 showed significantly less ability of IL-8 induction than G27ΔcagL/cagL26695 while displayed similar abilities of CagA phosphorylation, and cell elongation. Taken together, this study suggests that the CagL polymorphism may influence IL-8 induction, and K74 CagL has less ability to induce IL-8 secretion than G27 or 26695 CagL. Further research should address how the different capabilities of IL-8 induction between intraspecies-CagL are associated with the large differences of the incidence of gastric cancer between East Asian and Western countries.
Effect of Areca Nut on Helicobacter pylori-Induced Gastric Diseases in Mice
( Jinwook Lee ),( Niluka D Gunawardhana ),( Sungil Jang ),( Yun Hui Choi ),( Rasika P Illeperuma ),( Aeryun Kim ),( Hanfu Su ),( Youngmin A Hong ),( Ji-hye Kim ),( Jinmoon Kim ),( Da-woon Jung ),( In- 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.10
Areca nut (AN) chewing is a habit in many countries in Central, Southern, and Southeast Asia. It is strongly associated with the occurrence of oral, pharyngeal, and esophageal cancer as well as systemic inflammation. However, the association between AN intake and the development of gastric lesions has not yet been identified. The aim of this study was to investigate the effect of AN on gastric diseases using a mouse model for Helicobacter pylori infection. We studied four groups of mice: those fed a normal diet (ND), those fed a diet containing 2.5% AN (AD), those fed ND and infected with H. pylori PMSS1 strain (ND/HP), and those fed AD and infected with H. pylori PMSS1 strain (AD/HP). Food intake and body weight were monitored weekly during the experiments. At 10 weeks, the mice were sacrificed, and the stomach weight, H. pylori colonization, and gastric inflammation were evaluated. The stomach weight had increased significantly in the ND/HP and AD/HP groups along with increases in H. pylori colonization; however, there was no significant difference between these two groups with respect to stomach weight and colonization. On histological grading, mononuclear cell infiltration was severer in the AD/HP group than in the ND/HP group. These data suggest that chronic gastric inflammation was aggravated by AN treatment in the mice with H. pyloriinduced gastric lesions. Furthermore, as previously suggested, this animal model is useful to determine the effect of potential carcinogens on gastric lesions induced by H. pylori infection.
이경열(Kyungryol Lee),김진문(Jinmoon Kim),장재덕(Jaeduk Jang),주인식(Insik Joo) 한국자동차공학회 2009 한국자동차공학회 학술대회 및 전시회 Vol.2009 No.11
Cover plate for torque converter supports axial force generated by automatic transmission fluid circulation. Cover plate is fixed on front cover by projection welding. Welding defect is generated by the mismatch between a manufactured material and a method of production in manufacturing process. One of them is the case that the size of welding nugget is small. This research studied the effect of welding defect through finite element analysis. The concentration of stress usually happened in case the welding nugget formed is small. As a result, this part of production is brittle and fragile.
권기보(Kibo Kwon),김진문(Jinmoon Kim),장재덕(Jaeduk Jang),주인식(Insik Joo) 한국자동차공학회 2011 한국자동차공학회 학술대회 및 전시회 Vol.2011 No.11
Torque Converter located inside auto-transmission transmits power from engine to drive shaft during driving. The automatic transmission vehicles have many advantages same as convenience, speed of the smoothness and low-speed impact. In spite of many advantages compared with manual transmission, power transferred by fluid has inevitable drawbacks. Lock-up Clutch improving this loss, when driving a vehicle at the high speed, has a similar structure and characteristic of clutch of manual transmission as a device for a direct connection. When combined the Lock-up clutch, maximum efficiency is obtained.. But NVH is raised due to vibration and impact caused by the Lock-up device. Therefore various damper system is under development to solve these problems. Also, In addition to the goal of reducing NVH problems, It is greatly important to reduce the effect of oscillation, friction, and impact in damper’s service life. In this study, we propose the design approach to improve durability through the case study of stress distribution according to shapes of cut using Ansys Workbench in one of the commercial structure analysis programs.