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Kim, Ji-Eun,Lee, Sukmook,Han, Kyou-Sup,Kim, Hyun Kyung Lippincott Williams Wilkins, Inc. 2011 Blood coagulation & fibrinolysis Vol.22 No.2
Activation of the vascular endothelium and increased adhesion of circulating leukocytes to the activated endothelium are important events in inflammation and coagulation. Aurintricarboxylic acid (ATA), a triphenylmethyl dye compound, is known to inhibit platelet adhesion by interfering with the binding of von Willebrand factor to platelet glycoprotein Ib. However, the effect of ATA on the inflammatory response of endothelial cells has not yet been investigated. Here, we investigated the functional role and molecular mechanism of ATA on the activation of human endothelial cells. ATA inhibited the expression of intercellular adhesion molecule-1 (ICAM-1), and endothelial cell selectin (E-selectin) was upregulated on human umbilical vein endothelial cells (HUVECs) in response to tumor necrosis factor-α or lipopolysaccharide (LPS). We also observed the inhibitory effect of ATA on LPS-induced mRNA expression of ICAM-1 and E-selectin. Furthermore, ATA inhibited the binding of leukocytes to activated HUVECs. ATA significantly inhibited the nuclear translocation of nuclear factor-&kgr;B (NF-&kgr;B) and degradation of I&kgr;B on activated HUVECs, suggesting that ATA inhibits NF-&kgr;B signaling. Finally, three NF-&kgr;B inhibitors effectively inhibited the expressions of ICAM-1 and E-selectin on activated endothelial cells. The present data suggest that ATA exerts beneficial effect in various inflammation conditions through inhibition of adhesion molecule expression in activated endothelial cells and the resulting inhibition of leukocytes tissue accumulation.
VPL-DBS on neuropathic pain rat model is effective in mechanical allodynia than cold allodynia.
Kim, Jaehyung,Kim, Jinhyung,Min, Kyou Sik,Lee, Sung Eun,Kim, Sung June,Chang, Jin Woo Springer-Verlag Italia 2012 Neurological sciences Vol.33 No.6
<P>Recently, deep brain stimulation (DBS) is widely used in various types of neurodegenerative disorders for minimal invasiveness and safety of the procedure. Deep brain stimulation is consistently applied for the treatment of patients with neuropathic pain even though the success rate is not as high as other neurodegenerative disorders. Furthermore, it is also unclear how DBS improves neuropathic pain. In this study, we investigated the role of DBS following the stimulation parameter for analgesic effect on mechanical allodynia and cold allodynia in neuropathic pain rats. We used a sciatic nerve injury model to induce neuropathic pain, and observed responses to mechanical and cold stimulation by the von Frey test and acetone test, respectively. We classified the rats into four groups: na?ve (na?ve, n = 10), na?ve + DBS (N + DBS, n = 10), neuropathic pain (NP, n = 10), and neuropathic pain + DBS (NP + DBS, n = 10). We inserted the DBS electrode into the ventral posterolateral nucleus (VPL) into the rats (VPL-DBS). The score for mechanical allodynia was significantly decreased in NP + DBS group (p < 0.01). However, the score for cold allodynia did not significantly drop in any groups including NP + DBS group (p > 0.05). In this study, we found that the electrical stimulation of the VPL works more effectively with mechanical allodynia than cold one, and pain signal induced by mechanical stimulus and cold stimulus may be processed through different pathways in the brain.</P>
Kim, Hyun Kyung,Kim, Ji-Eun,Chung, Junho,Kim, Young Tae,Kang, Seong-Ho,Han, Kyou-Sup,Cho, Han-Ik Rapid Communications of Oxford Ltd 2007 Blood coagulation & fibrinolysis Vol.18 No.2
Thrombomodulin has a central role in the regulation of coagulation through its abilities to promote generation of the potent anticoagulant activated protein C. Because little is known about monocyte thrombomodulin expression and its regulatory mechanism by lipopolysaccharide, we investigated the effect of lipopolysaccharide on monocyte’s thrombomodulin expression. Lipopolysaccharide reduced the surface thrombomodulin expression of human peripheral blood monocytes in a dose-dependent and time-dependent manner, regardless of the addition of serum. The surface thrombomodulin activity was comparably decreased in monocytes incubated with lipopolysaccharide. Blocking nuclear factor-&kgr;B by MG132 or aurine tricarboxylic acid effectively inhibited the lipopolysaccharide-induced surface thrombomodulin down-regulation of monocytes. Lipopolysaccharide inactivation by polymyxin B in the supernatants from the lipopolysaccharide-stimulated cultures still reduced the surface thrombomodulin expression of monocytes, suggesting a role for soluble mediators in the down-regulation of thrombomodulin. The lipopolysaccharide-induced thrombomodulin surface expression and the mRNA levels of the monocytic leukemic cell line (THP-1) were decreased in serum-depleted culture, while they were increased in medium containing 10% serum. We conclude that lipopolysaccharide down-regulates the thrombomodulin expression of monocytes and that nuclear factor-&kgr;B is a critical mediator of the repression of thrombomodulin by lipopolysaccharide. Regulation of the THP-1 thrombomodulin expression by lipopolysaccharide depends on the presence of serum.
아리랑위성 2호, 5호의 우주파편 충돌회피기동 주기 분석
김은혁(Eun-Hyouek Kim),김해동(Hae-Dong Kim),김은규(Eun-Kyou Kim),김학정(Hak-Jung Kim) 한국항공우주학회 2011 韓國航空宇宙學會誌 Vol.39 No.11
본 논문에서는 현재 운용 중인 아리랑위성 2호와 발사예정인 아리랑위성 5호의 우주파편 충돌 회피기동 주기를 분석하였다. 이때, 각 위성들의 임무궤도 특성, 충돌 회피 여유시간, 허용 충돌확률, 위치 불확실성 등의 인자들의 변화에 따라 분석을 수행하였다. 또한, 결과의 타당성을 검증하기 위해 실제 1년 동안 생성된 NORAD TLE 카탈로그(catalog) 상의 우주 물체들과 아리랑위성 2호와의 충돌 회피기동 주기를 계산하였다. 분석 결과, 두 위성 모두 연중 약 1회 충돌 회피기동이 요구됨을 확인할 수 있었으며, 계산 인자들의 변화에 따른 결과 분석을 통해 추후 발사 예정인 저궤도 위성들의 충돌 회피기동 주기 예측 정밀도를 향상시키기 위한 방안들을 제시하였다. In this paper, a collision avoidance maneuver frequency for the KOMPSAT-2 and the KOMPSAT-5 is analyzed. For the statistical prediction of the avoidance maneuver frequency, mission orbits, responsive time, accepted collision probabilities, and positional uncertainties of primary and secondary objects are considered. In addition, the collision avoidance maneuver frequency of the KOMPSAT-2 is compared to the case that NORAD catalog during one year is used to calculate that of the KOMPSAT-2. As a result, the collision avoidance maneuver frequency is one per year on average and effective factors on the statistical prediction of the avoidance maneuver frequency are investigated. Efforts to improve its prediction accuracy are also discussed.
( Min Ji Kim ),( Dong Hyuk Eun ),( Kyou Chae Lee ),( Yong Hyun Jang ),( Weon Ju Lee ),( Do Won Kim ),( Ji Yoon Kim ),( Seok Jong Lee ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.1
Kasabach-Merritt phenomenon(KMP) which is one of possible life-threatening complications of kaposiform hemangioendothelioma(KHE) has several therapeutic options but the standard therapy for KHE has not set up yet. However, several studies reported recently effectiveness of sirolimus, which is a mammalian target of Rapamycin (mTOR) inhibitor has been used extensively in children following solid organ transplantation. A 2-month-old female presented with a tender dusky purplish tumor (6x7cm) on the left upper arm since 1-month-old. Histopathological finding was consistent with KHE in addition to pathognomonic kaposiform vascular area. So we diagnosed KEA. By clinical-laboratory findings, patient was checked anemia(Hb 8.6g/dl), thrombocytopenia(platelet 13k/uL), elevated D-dimer, decreased fibrinogen, necessitating hospitalization. After admission to pediatrics, she was treated using antibiotics IV, propranolol PO, steroid IV, vincristine IV, IVIG during hospitalization for 2 months. But the lesion tended to wax and wane. So clinical trial by sirolimus 0.1mg/kg/day after informed consent showed significant clinical improvement. Within 14 days of commending sirolimus, the platelet count had risen to normal range (135k/ul). Presently she is receiving 0.5 mg/kg/day with complete response. We report a successful therapy using oral sirolimus for life-threatening KHE complicated with KMP, which failed to respond to multiple interventions.
Impact of cytokine gene polymorphisms on risk and treatment outcomes of aplastic anemia
Lee, Yun-Gyoo,Kim, Inho,Kim, Jin Hee,Bae, Ji-Yeon,Kwon, Ji-Hyun,Shin, Dong-Yeop,Lee, Jong-Eun,Song, Eun Young,Kim, Hyun Kyoung,Yoon, Sung-Soo,Park, Sung Sup,Lee, Dong Soon,Han, Kyou-Sup,Park, Myoung H Springer-Verlag 2011 Annals of hematology Vol.90 No.5
Intranuclear delivery of synthetic nuclear factor-kappa B p65 reduces inflammasomes after surgery
Cheon, So Yeong,Kim, Jeong Min,Kim, Eun Jung,Kim, So Yeon,Kam, Eun Hee,Ho, Chun-Chang,Lee, Sang-Kyou,Koo, Bon-Nyeo Elsevier 2018 Biochemical pharmacology Vol.158 No.-
<P><B>Abstract</B></P> <P>Patients undergoing surgery can suffer from various complications, including post-operative bleeding, local or systematic infection, and neurologic disorders. Major surgery can initiate innate immune responses and trigger overproduction of inflammatory mediators, which can contribute to organ dysfunction. Inflammasomes are innate immune complexes, which are connected to the pathogenesis of various diseases, including atherosclerosis, hemorrhagic brain injury, and Alzheimer’s disease. In the present study, we hypothesized that nucleotide-binding oligomerization domain-containing-like receptor protein (NLRP) inflammasomes may have a role in the pathological effects of surgery. Therefore, we designed a protein inhibitor of nuclear factor kappa B (NF-κB) p65 transcripts, called nt-p65-TMD (nuclear transducible (nt) transcription modulated domain (TMD) of RelA (p65)), that can penetrate the nucleus, and evaluated its therapeutic efficacy for dampening surgery-induced inflammasome activation. It was found that the nt-p65-TMD significantly reduced the NLRP1 inflammasome complex components (NLRP1, ASC, and Caspase-1) and interleukin (IL)-1β and IL-18 productions in the spleen after surgery. In the spleen, specific cell population and selective mediators were altered after surgery with/without nt-p65-TMD treatment. Also, we found that treatment of nt-p65-TMD decreased cell death in the spleen after surgery. Therefore, nt-p65-TMD is a potential novel strategy for reducing surgery-induced NLRP1 inflammasome and complications.</P> <P><B>Graphic abstract</B></P> <P>Treatment with synthetic NF-κB p65 protein (nt-p65-TMD) is a novel strategy of targeting surgical injury-induced NLRP1 inflammasome and complications for maintaining immune homeostasis.</P> <P>[DISPLAY OMISSION]</P>
정윤중,김혜현,김은한,김지연,차세정,김유진,강정은,정연화,정영선,김영환,경규혁,홍석경,Jung, Yooun-Joong,Kim, Hea-Hyun,Kim, Eun-Han,Kim, Ji-Yeoun,Cha, Se-Jung,Kim, You-Jin,Kang, Jung-Eun,Chung, Yeon-Hwa,Jung, Young-Sun,Kim, Young-Hwan,Kyou 한국의료질향상학회 2014 한국의료질향상학회지 Vol.20 No.1
Objective: The objective of this research was to develop a guideline for more effective use of physical restraint on patients in the intensive care unit and training the nurses on it and applying it on clinical practice to assess its effectiveness. Method: This research analyzed the before and after effect of the development of a guideline for physical restraint by dividing the category into nurse and patient. In the case of nurse, a comparison of knowledge and nursing service regarding the use of physical restraint from before the training on physical restraint guideline(Jan. 2011) and after the training on physical restraint guideline(Dec. 2011) was made. In the case of patient, a comparison of physical restraint usage rate and average usage time, the number of unplanned extubation cases were compared from before the use of physical restraint (Jan.~Apr. 2011) and after the use of physical restraint (Sep.~Dec. 2011) were made. Result: After the training on the physical restraint guideline, the knowledge of the nurse and the nursing practice showed notable improvement by (p<0.000) and (p<0.048) respectively and in patient, physical restraint usage rate and average time of usage decreased by (p<0.001) and (p<0.001) respectively. And despite the decrease in the number of cases in which the physical restraint was used, the number of unplanned extubation cases remained the same. Conclusion: Physical restraint guideline training and guideline usage can be stated to have brought out positive effect in both the nurse and patient. In order to maintain such positive effects, continuous training is necessary and continuous revaluation is necessary, regarding knowledge and nursing practices.