Effects of Cu,Zn-superoxide dismutase on cell proliferation and neuroblast differentiation in the mouse dentate gyrus.

Yoo, Dae Young,Shin, Bich Na,Kim, In Hye,Kim, Woosuk,Kim, Dae Won,Yoo, Ki-Yeon,Choi, Jung Hoon,Lee, Choong Hyun,Yoon, Yeo Sung,Choi, Soo Young,Won, Moo-Ho,Hwang, In Koo Kluwer Academic/Plenum Publishers 2012 Neurochem Res Vol.37 No.2

<P>Oxidative stress is one of the most important factors in reducing adult hippocampal neurogenesis in the adult brain. In this study, we observed the effects of Cu,Zn-superoxide dismutase (SOD1) on lipid peroxidation, cell proliferation, and neuroblast differentiation in the mouse dentate gyrus using malondialdehyde (MDA), Ki67, and doublecortin (DCX), respectively. We constructed an expression vector, PEP-1, fused PEP-1 with SOD1, and generated PEP-1-SOD1 fusion protein. We administered PEP-1 and 100 or 500 관g PEP-1-SOD1 intraperitoneally once a day for 3 weeks and sacrificed at 30 min after the last administrations. PEP-1 administration did not change the MDA levels compared to those in the vehicle-treated group, while PEP-1-SOD1 treatment significantly reduced MDA levels compared to the vehicle-treated group. In the PEP-1-treated group, the number of Ki67-positive nuclei was similar to that in the vehicle-treated group. In the 100 관g PEP-1-SOD1-treated group, the number of Ki67-positive nuclei was slightly decreased; however, in the 500 관g PEP-1-SOD1-treated group, Ki67-positive nuclei were decreased to 78.5% of the vehicle-treated group. The number of DCX-positive neuroblasts in the PEP-1-treated group was similar to that in the vehicle-treated group. However, the arborization of DCX-positive neuroblasts was significantly decreased in both the 100 and 500 관g PEP-1-SOD1-treated groups compared to that in the vehicle-treated group. The number of DCX-positive neuroblasts with tertiary dendrites was markedly decreased in the 500 관g PEP-1-SOD1-treated group. These results suggest that a SOD1 supplement to healthy mice may not be necessary to modulate cell proliferation and neuroblast differentiation in the dentate gyrus.</P>

Effects of Streptozotocin-Induced Type 1 Diabetes on Cell Proliferation and Neuronal Differentiation in the Dentate Gyrus : Correlation with Memory Impairment

Jung Hoon Choi,In Koo Hwang,Sun Shin Yi,Ki-Yeon Yoo,Choong Hyun Lee,Hyung-Cheul Shin,Yeo Sung Yoon,Moo-Ho Won 대한해부학회 2009 Anatomy & Cell Biology Vol.42 No.1

We examined the effects of steptozotocin (STZ)-induced type 1 diabetes on cell proliferation and neuroblasts in the subgranular zone of the hippocampal dentate gyrus (SZDG) of male Wistar rats. Change in memory function was also investigated using the passive avoidance test. In the SZDG, Ki67 (a marker for cell proliferation) positive nuclei were significantly decreased at 2 and 3 weeks and slightly decreased at 4 weeks after STZ treatment. Doublecortin (DCX, a marker for neuronal differentiation)-immunoreactive (+) neuroblasts with tertiary dendrites were significantly decreased in the STZ-treated group compared to those in the vehicle-treated group. However, DCX+ neuroblasts without tertiary dendrites were abundant at 4 weeks after STZ treatment. In addition, retention latency time in STZ-treated group was similar to that of vehicle-treated group at 2 and 3 weeks after STZ treatment. However, the retention latency time was significantly decreased at 4 weeks after STZ treatment. These results suggest that STZ significantly reduced cell proliferation and neuroblasts at 2~3 weeks after STZ treatment, but not at 4 weeks after STZ treatment although memory impairment was detected at 4 weeks after STZ treatment. The gradual reduction of DCX+ neuroblasts with tertiary dendrites may be associated with the impairment of hippocampus-related memory function.

Effects of <i>Ginkgo biloba</i> Extract on Promotion of Neurogenesis in the Hippocampal Dentate Gyrus in C57BL/6 Mice

YOO, Dae Young,NAM, YoonYi,KIM, Woosuk,YOO, Ki-Yeon,PARK, Jaeil,LEE, Choong Hyun,CHOI, Jung Hoon,YOON, Yeo Sung,KIM, Dong-Woo,WON, Moo-Ho,HWANG, In Koo Japanese Society of Veterinary Science 2011 The Journal of veterinary medical science Vol.73 No.1

<P><I>Ginkgo biloba </I>leaf extract (Gb) has been known to improve blood flow and preclude the tissue from free radical damage. Effects of Gb were examined by using Ki67, a specific proliferative marker for cellular proliferation, and doublecortin (DCX), a marker for immature neurons, indicating degree of neuroblast differentiation in the hippocampal dentate gyrus (DG) of adult C57BL/6 mice. The mice were fed with Gb at 40 and 100 mg/kg once daily for 28 days. The increase of Ki67- and DCX-immunoreactive cells in the DG was increased in a dose-dependent manner. Especially, the group having 100 mg/kg Gb showed a significant increase of DCX-immunoreactive neuroblasts with well-developed tertiary dendrites. Expression of DCX protein in the Gb groups was also significantly increased upon compared with the vehicle group. The results suggested that repeated intake of Gb would enhance cell proliferation and neuroblast differentiation in the mouse DG.</P>

Cell proliferation and neuroblast differentiation in the rat dentate gyrus after intrathecal treatment with adipose-derived mesenchymal stem cells.

Choi, Jung Hoon,Chung, Jin Young,Yoo, Dae Young,Hwang, In Koo,Yoo, Ki-Yeon,Lee, Choong Hyun,Yan, Bing Chun,Ahn, Jin Ok,Youn, Hwa Young,Won, Moo-Ho Kluwer Academic/Plenum Publishers 2011 Cellular and molecular neurobiology Vol.31 No.8

<P>Mesenchymal stem cells (MSC) have emerged as a new therapeutic tool for a number of clinical applications, because they have multipotency and paracrine effects via various factors. In the present study, we investigated the effects of adipose-derived MSC (Ad-MSC) transplantation via intrathecal injection through the cisterna magna on cell proliferation and differentiation of endogenous stem cells in the hippocampal dentate gyrus (DG) using Ki-67 (a marker for proliferating cells), and doublecortin (DCX, a marker for neuroblasts). The transplanted Ad-MSC were detected in the meninges, not in the hippocampal parenchyma. However, the number of Ki-67-immunoreactive cells was significantly increased by 83% in the DG 2 days after single Ad-MSC injection, and by 67% at 23 days after repeated Ad-MSC treatment compared with that in the vehicle-treated group after Ad-MSC transplantation. On the other hand, the number of DCX-immunoreactive cells in the DG was not changed at 2 days after single Ad-MSC injection; however, it was significantly increased by 62% 9 days after single Ad-MSC injection. At 23 days after repeated Ad-MSC application, the number of DCX-immunoreactive cells was much more increased (223% of the vehicle-treated group). At this time point, DCX protein levels were also significantly increased compared with those in the vehicle-treated group. These results suggest that the intrathecal injection of Ad-MSC could enhance endogenous cell proliferation, and the repeated Ad-MSC injection could be more efficient for an enhancement of endogenous cell proliferation and differentiation in the brain.</P>

The high dosage of earthworm (Eisenia andrei) extract decreases cell proliferation and neuroblast differentiation in the mouse hippocampal dentate gyrus

Bing Chun Yan,Ki-Yeon Yoo,Joon Ha Park,Choong Hyun Lee,Jung Hoon Choi,Moo-Ho Won 대한해부학회 2011 Anatomy & Cell Biology Vol.44 No.3

Earthworm extract has shown anticancer characteristics. In the present study, we examined the effect of chronic treatment with a high dose of earthworm (Eisenia andrei) extract (EE) on cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus (DG) of 3-week-old mice using 5-bromo-2’-deoxyuridine (BrdU) and Ki-67 immunohistochemistry for cell proliferation and doublecortin (DCX) immunohistochemistry for neuroblast differentiation, respectively. BrdU-, Ki-67-, and DCX-immunoreactive cells were easily detected in the subgranular zone of the DG in vehicle (saline)-treated mice. However, BrdU-, Ki-67-, and DCX-immunoreactive cells in the 500 ㎎/㎏ EE-treated mice decreased distinctively compared to those in the vehicle-treated mice. In addition, brain-derived neurotrophic factor (BDNF) immunoreactivity and its protein level decreased markedly in the DG of the EE-treated group compared to those in the vehicle-treated group. These results indicate that chronic treatment with high dose EE decreased cell proliferation and neuroblast differentiation, and that BDNF immunoreactivity decreased in the DG of EE-treated mice.

The high dosage of earthworm (Eisenia andrei) extract decreases cell proliferation and neuroblast differentiation in the mouse hippocampal dentate gyrus.

Yan, Bing Chun,Yoo, Ki-Yeon,Park, Joon Ha,Lee, Choong Hyun,Choi, Jung Hoon,Won, Moo-Ho Korean Association of Anatomists 2011 Anatomy & Cell Biology Vol.44 No.3

<P>Earthworm extract has shown anticancer characteristics. In the present study, we examined the effect of chronic treatment with a high dose of earthworm (Eisenia andrei) extract (EE) on cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus (DG) of 3-week-old mice using 5-bromo-2'-deoxyuridine (BrdU) and Ki-67 immunohistochemistry for cell proliferation and doublecortin (DCX) immunohistochemistry for neuroblast differentiation, respectively. BrdU-, Ki-67-, and DCX-immunoreactive cells were easily detected in the subgranular zone of the DG in vehicle (saline)-treated mice. However, BrdU-, Ki-67-, and DCX-immunoreactive cells in the 500 mg/kg EE-treated mice decreased distinctively compared to those in the vehicle-treated mice. In addition, brain-derived neurotrophic factor (BDNF) immunoreactivity and its protein level decreased markedly in the DG of the EE-treated group compared to those in the vehicle-treated group. These results indicate that chronic treatment with high dose EE decreased cell proliferation and neuroblast differentiation, and that BDNF immunoreactivity decreased in the DG of EE-treated mice.</P>

작업조건과 정신신체 자각증상의 관련성

정영호,유기하,이영수,이동배 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.1

To investigate the relationship between work characteristics and psychosomatic symptoms, the author conducted survey for 1,618 workers who employed at manufacturing factories, located in Taejon city and Chungnam region. The following results were obtained ; Among the work characteristics, job classification, duration of work, work hours per day, work intensity, working environment, and working climate showed significant relationship with the psychosomatic symptoms scores. After sociodemographic variables were controlled, the variables of above work characteristics, except work hours per day, showed statistically signicant difference. In the stepwise multiple regression of symptom scores, the significantly related variables which were work intensity, working environment, working climate selected in that order.

Cytokinin과 대두(Glycine max)잎단백질의 결합에 대하여

류기중,박창규,정창조 제주대학교 방사능이용연구소 1986 연구보고 Vol.2 No.-

cytokinin과 단백질의 결합을 간단하게 검정하는 방법으로서 전기영동법을 시도하고, 대두의 잎단백질과 cytokinin의 결합여부, cytokinin에 대한 affinity가 있는 단백질의 종류와 상대적 affinity를 조사하였다. 검토된 전기영동법은 cytokinin과 단백질의 결합 뿐만아니라, cytokinin에 대한 상대적 affinity를 동시에 검정할 수 있는 장점을 가지고 있었다. Ammonium sulfate 침전법, Sephadex G-25 chromatography, paper chromatography, 그리고 전기영동으로 대두의 잎단백질 중에 BA와 결합하는 단백질이 있음을 확인할 수 있었다. 전기영동법으로 검정한 결과 BA와 결합하는 것이 3 group이 있고, 이중에서 전기영동 이동도로 보아 분자량이 작은 단백질 분획과 전기영동 이동도 0.4부근의 분획은 BA에 대한 affinity가 비교적 낮은 반면, 이동도 0.0∼0.2의 분획은 affinity가 큰 것으로 생각되었다. A polyacrylamide gel electrophoresis technique was applied to cytokinin-protein binding assay. Binding of soybean leaf proteins to cytokinin and relative affinities of protein fractions to cytokinin were studied. The electrophoresis technique appeared to be very useful for determination of cytokinin-protein binding, for identification of protein species binding to cytokinin, and for comparison of relative affinities of the proteins to cytokinin. The presence of cytokinin-binding proteins in soybean leaves was confirmed from assays with ammonium sulfate precipitation, Sephadex G-25 chromatography, paper chromatography, and electrophoresis. Three groups of cytokinin-binding proteins were identified in the soybean leaf protein extract and two of the three showed low affinity to cytokinin, however, the third one with mobility between 0.0∼0.2, probably high molecular weight protein (s), showed high affinity in the electrophoretic analysis.

당근 Protoplast에 관한 기초연구

유기중,유장걸 제주대학교 1985 논문집 Vol.20 No.-

Protoplast of carrot were isolated and purified from leaves, roots, and callus of root cambium cultured on MS medium. in order to investigate some basic characteristics of the carrot protoplasts. 1. During enzyme treatment. non-shaking brought about higher yield of protoplasts than shaking by which many protoplasts were destroyed. 2. The sizes of the isolated protoplasts were variable, root protoplasts were largest and leaf protoplasts smallest 3. No color particle was observed in callus protoplasts. but green color in leaf and orange in root. 4. Protoplast densities of leaf and callus were slightly increased after 2-day incubation with the treatment of kinetin and BA. while decreased with the treatmnent of GA. NAA and IAA. Cell wall formation was obserbed from each treatment of growth regulator after 2-day incubation. 5. The protoplast fusions of leaf-root. and leaf-callus were easily recognized in virtue of their typical color and size. The degree of protoplast fusion obtained by PEG eluting method was 1-5%. higher than by the centrifugal method.

성신여자대학교 교내 및 학교 주변 음용수 검사

유은아,김정수,민혜기 誠信女子大學校 1998 硏究論文集 Vol.36 No.-

성신여자대학교 교내 및 주변 음식점의 물이 음용수로써 적합한지의 여부를 검사하였다. 검수 속의 금속 및 중금속이온은 원자흡수분광법을, 무기 음이온은 이온쌍 크로마토그래피법을, 그리고 휘발성 유기물질은 Purge & Trap과 기체크로마토그래피법을 각각 이용하여 분석하였으며 물속에 들어 있는 대장균과 일반세균의 검출은 계절별로 실시하였다. 교내 검수 결과 대부분의 경우 금속 및 중금속 이온, 무기물 음이온, 휘발성 유기물질들은 환경부에서 제시한 기준치 이하로 검출되었으며 세균의 경우 일반 세균의 양은 기준치를 약간 넘는 양이었으나 대장균은 검출되지 않았다. 정수기를 통과한 물인 경우 일반 세균의 수가 감소한 것으로 보아 정수기의 필터만 잘 관리한다면 일반 세균까지도 제거할 수 있다는 장점을 나타내었다. 학교 주변의 경우도 대부분 기준치 이하로 존재하였으나 세균검사의 경우 여름철로 들어서서는 일반 세균의 검출수도 많아졌으며 특히 대장균이 검출된 경우도 있었다. Analysis of drnking water in and around Sungshin Women's University was carried out using Atomic absorption spectrometer for metal and heavy metal ion, Ion pair chromatography system for inorganic anions, and Purge & Trap and Gas chromatography system for volatile organic compounds. In addition we also measured the drinking water for bacteria and coli each season. Most chemical species in the school's drinking water tested below the maximum levels suggested by Korea's Ministry of Environment. Moreover, the number of bacterial colony is just above the suggested levels and there is no detection of coli. Around school the results were almost identical; however in the summer, some of the sampling water showed coli and an increase of bacterial colonies. The most significant result is there is much less or no detection of bacteria and coli in drinking water from clean sources than in water from unclean sources.