Subcellular Localization of Diacylglycerol-responsive Protein Kinase C Isoforms in HeLa Cells

Kazi, Julhash U.,Kim, Cho-Rong,Soh, Jae-Won Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.9

Subcellular localization of protein kinase often plays an important role in determining its activity and specificity. Protein kinase C (PKC), a family of multi-gene protein kinases has long been known to be translocated to the particular cellular compartments in response to DAG or its analog phorbol esters. We used C-terminal green fluorescent protein (GFP) fusion proteins of PKC isoforms to visualize the subcellular distribution of individual PKC isoforms. Intracellular localization of PKC-GFP proteins was monitored by fluorescence microscopy after transient transfection of PKC-GFP expression vectors in the HeLa cells. In unstimulated HeLa cells, all PKC isoforms were found to be distributed throughout the cytoplasm with a few exceptions. PKC$\theta$ was mostly localized to the Golgi, and PKC$\gamma$, PKC$\delta$ and PKC$\eta$ showed cytoplasmic distribution with Golgi localization. DAG analog TPA induced translocation of PKC-GFP to the plasma membrane. PKC$\alpha$, PKC$\eta$ and PKC$\theta$ were also localized to the Golgi in response to TPA. Only PKC$\delta$ was found to be associated with the nuclear membrane after transient TPA treatment. These results suggest that specific PKC isoforms are translocated to different intracellular sites and exhibit distinct biological effects.

Population Panmixia and the Pleistocene Demographic Expansion of Spotty Belly Greenling Hexagrammos agrammus in the East Sea and Northwest Pacific

Kazi Ahsan Habib,정다금,명정구,이윤호 한국해양과학기술원 2015 Ocean science journal Vol.50 No.2

The population genetic structure and historical demography of spotty belly greenling, Hexagrammos agrammus, which has limited distribution in the Northwest Pacific, was assessed with partial sequences of the mitochondrial DNA (mtDNA) cytochrome b and the control region (D-loop). A total of 103 individuals were collected from four sites located at the Korea Strait (Southern coast of Korea) and the East coast of Korea and two places in the Pacific coast of Japan. For all the populations, nucleotide diversities were low (0.006 - 0.009) while the haplotype diversities were as high as 0.92 to 0.97, indicating that the fish has undergone a recent population expansion after experiencing bottleneck. Star-shaped patterns of haplotype networks as well as the significant negative values of Tajima’s D and Fu’s FS corroborate the recent population expansion. Mismatch distribution analysis reveals that the demographic expansion of the species started during the 2nd half of the Middle Pleistocene Series approximately 141,000 - 406,000 years ago. Hierarchical analysis of molecular variance (AMOVA), the pairwise population statistics (FST), and the exact test of haplotype differentiation demonstrate no significant genetic differentiation among populations investigated, suggesting that spotty belly greenling is panmictic in the East Sea and the Pacific coast of Japan.

Population genetic structure and demographic history of the fat greenling Hexagrammos otakii

Kazi Ahsan Habib,정다금,Jung-Goo Myoung,Min Seok Kim,장요순,Jae Seol Shim,Youn-Ho Lee 한국유전학회 2011 Genes & Genomics Vol.33 No.4

Fat greenling (Hexagrammos otakii) is an important commercial fish in the Northwestern Pacific, being distributed along the coastal waters of the East/Japan Sea and the Yellow Sea. To investigate population genetic structure and demographic history of this species, one hundred and fifty five individuals were collected from five localities in the distribution range of the species and sequence variations in the mitochondrial genes COI, COIII-ND3-ND4L, and cytochrome b were examined. For all the genes in every sampling location, the nucleotide diversities were very low (0.001 ~ 0.005) although the haplotype diversities were relatively high, 0.55 ~ 0.81 for COI, 0.79 ~ 0.84 for COIII-ND3-ND4L, and 0.95 ~ 0.97 for cytochrome b. Hierarchical analysis of molecular variance (AMOVA), the conventional population statistic FST, and exact test of population differentiation revealed no significant genetic structuring among the samples, indicating that fat greening is panmictic throughout the range of its distribution. Neutrality tests such as Tajima’s D and Fu’s FS statistics and mismatch distribution analyses suggested that fat greening has undergone the demographic history of population expansion during the late Pleistocene period approximately 91,000 ~327,000 years ago. The star-burst patterns of haplotype networks and low nucleotide diversities also indicated recent population expansion. These results help establish the fisheries management strategy for fat greenling in the Northwestern Pacific.

Assessment of Land Cover Changes from Protected Forest Areas of Satchari National Park in Bangladesh and Implications for Conservation

Kazi Mohammad Masum,Md. Mehedi Hasan 강원대학교 산림과학연구소 2020 Journal of Forest Science Vol.36 No.3

Satchari National Park is one of the most biodiverse forest in Bangladesh and home of many endangered flora and fauna. 206 tons of CO2 per hectare is sequestrated in this national park every year which helps to mitigate climate issues. As people living near the area are dependent on this forest, degradation has become a regular phenomenon destroying the forest biodiversity by altering its forest cover. So, it is important to map land cover quickly and accurately for the sustainable management of Satchari National Park. The main objective of this study was to obtain information on land cover change using remote sensing data. Combination of unsupervised NDVI classification and supervised classification using maximum likelihood is followed in this study to find out land cover map. The analysis showed that the land cover is gradually converting from one land use type to another. Dense forest becoming degraded forest or bare land. Although it was slowed down by the establishment of ‘National Park’ on the study site, forecasting shows that it is not enough to mitigate forest degradation. Legal steps and proper management strategies should be taken to mitigate causes of degradation such as illegal felling.

Ecotourism Carrying Capacity and the Potentiality of the Safari Park of Bangladesh

Kazi Mohammad Masum,Abdullah Al Mamun,Zahed Mohammad Malekur Rahman,Md. Motlubur Rahman,Md. Shah Newaz,Mohammad Redowan 강원대학교 산림과학연구소 2013 Journal of Forest Science Vol.29 No.4

The study was undertaken to assess the tourist’s carrying capacity and to explore ecotourism potential of Bangabandhu Sheikh Mujib Safari Park, the only safari park of Bangladesh. Carrying capacity was assessed both from physical and social aspect. On the other hand, 250 visitors were interviewed with semi structured questionnaire for ecotourism prospect evaluation along with some secondary data. The total daily-allowed visit was 17,300 with rotation coefficient of 3.46. Maximum daily average numbers of tourists were relatively below the physical carrying capacity of the Park (5,000). The social carrying capacity was 4.38 persons per day. As there are different categories of visitors, their choice of encounters and number of expected encounters also varied. Each year, the total tourist’s number varied significantly (p≤0.05). That proved the safari park had immense tourist prospect. 85 percent visitors were from lower and middle class family as entrance fees and other fees inside were very cheap in relation to other private amusement place. The ecological perspective of the park will remain unaltered if the carrying capacities of the area are followed with sustainability. Therefore, it is the proper time for taking appropriate decision.

Chelonodontops bengalensis (Tetraodontiformes: Tetraodontidae): A New Species of Puffer Fish from the Northern Bay of Bengal Based on Morphology and DNA Barcode

Kazi Ahsan Habib,Amit Kumer Neogi,오진아,이윤호,김충곤 한국해양과학기술원 2019 Ocean science journal Vol.54 No.1

The new puffer fish species Chelonodontops bengalensis (Pisces: Tetraodontidae) is described from two specimens collected on the southwest coast of the Bay of Bengal, Bangladesh. C. bengalensis is closely related to C. patoca (Hamilton, 1822), both species being large puffers with a peculiar, disc-shaped exposed nasal organ. The new species however differs from C. patoca with respect to dorsal rays (12 vs 10), anal rays (10 vs 8), pectoral rays (19 vs 15–16) and gill rakers (8 vs 7). C. bengalensis has spinules on its lateral sides but not on the caudal peduncle (vs. a patch of spinules on the back from behind the interorbital nearly to the dorsal fin and another on the throat and abdomen but absent on the lateral sides in C. patoca). Both the DNA barcode region of the mitochondrial COI gene and 16S rRNA gene region of C. bengalensis showed a clear monophyletic clade that distinguishes it as a separate species in the phylogenetic tree from other closely related puffer fish species. The COI barcode region shows a genetic divergence of 6 to 7.6% between C. bengalensis and C. patoca reported in GenBank from different parts of Asia.

Impact of teachers' professional development on school improvement-an analysis at Bangladesh standpoint

Kazi Enamul Hoque,Gazi Mahabubul Alam,Abdul Ghani Kanesean Abdullah 서울대학교 교육연구소 2011 Asia Pacific Education Review Vol.12 No.3

This study seeks to describe the teachers' professional development activities in Bangladesh and explores the hypotheses about the relationship between teachers' traditional professional development activities and school improvement. Data from a representative sample of City secondary schools from Bangladesh (n = 127) were gathered through questionnaires from 127 principals and 694 teachers. Hierarchical multiple regression analysis was used in this research. This study found significant impacts of some of teachers' professional development activities on school improvement. Also found that the maximum school improvement can be achieved if schools put more emphasis on teachers' collaboration, in-service training and classroom observation and less emphasis on individual action enquiry. The findings of this study provide important information for the policy makers, educational managers and especially for the headmasters and teachers concerned with the improvement of teachers' quality in secondary schools of Bangladesh. This study adopts a concurrent approach of data collection and analysis.

Analysis of LinkedIn Jobs for Finding High Demand Job Trends Using Text Processing Techniques

Kazi, Abdul Karim,Farooq, Muhammad Umer,Fatima, Zainab,Hina, Saman,Abid, Hasan International Journal of Computer ScienceNetwork S 2022 International journal of computer science and netw Vol.22 No.10

LinkedIn is one of the most job hunting and career-growing applications in the world. There are a lot of opportunities and jobs available on LinkedIn. According to statistics, LinkedIn has 738M+ members. 14M+ open jobs on LinkedIn and 55M+ Companies listed on this mega-connected application. A lot of vacancies are available daily. LinkedIn data has been used for the research work carried out in this paper. This in turn can significantly tackle the challenges faced by LinkedIn and other job posting applications to improve the levels of jobs available in the industry. This research introduces Text Processing in natural language processing on datasets of LinkedIn which aims to find out the jobs that appear most in a month or/and year. Therefore, the large data became renewed into the required or needful source. This study thus uses Multinomial Naïve Bayes and Linear Support Vector Machine learning algorithms for text classification and developed a trained multilingual dataset. The results indicate the most needed job vacancies in any field. This will help students, job seekers, and entrepreneurs with their career decisions

A study on the unloading modulus of Al-alloy foam

Kazi Tunvir,Gil-Do Jeong,Amkee Kim,Seong-Sick Cheon,Byung-Wook Ahn 한밭대학교 생산융합기술연구소 2005 생산융합기술연구소 논문집 Vol.5 No.1

Role of Regulatory Domain Mutants of PKC Isoforms in c-fos Induction

Kazi, Julhash U.,Soh, Jae-Won Korean Chemical Society 2008 Bulletin of the Korean Chemical Society Vol.29 No.1