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Kasi, D.,Catherine, C.,Lee, S. W.,Lee, K. H.,Kim, Y. J.,Ro Lee, M.,Ju, J. W.,Kim, D. M. American Institute of Chemical Engineers 2017 Biotechnology progress Vol.33 No.3
<P>The rapidly evolving cloning and sequencing technologies have enabled understanding of genomic structure of parasite genomes, opening up new ways of combatting parasite-related diseases. To make the most of the exponentially accumulating genomic data, however, it is crucial to analyze the proteins encoded by these genomic sequences. In this study, we adopted an engineered cell-free protein synthesis system for large-scale expression screening of an expression sequence tag (EST) library of Clonorchis sinensis to identify potential antigens that can be used for diagnosis and treatment of clonorchiasis. To allow high-throughput expression and identification of individual genes comprising the library, a cell-free synthesis reaction was designed such that both the template DNA and the expressed proteins were co-immobilized on the same microbeads, leading to microbead-based linkage of the genotype and phenotype. This reaction configuration allowed streamlined expression, recovery, and analysis of proteins. This approach enabled us to identify 21 antigenic proteins. (C) 2017 American Institute of Chemical Engineers</P>
Poset Properties Determined by the Ideal - Based Zero-divisor Graph
Porselvi, Kasi,Elavarasan, Balasubramanian Department of Mathematics 2014 Kyungpook mathematical journal Vol.54 No.2
In this paper, we study some properties of finite or infinite poset P determined by properties of the ideal based zero-divisor graph properties $G_J(P)$, for an ideal J of P.