http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kim, Raeyeong,Kanamaru, Shuji,Mikawa, Tsutomu,Pré,vost, Chantal,Ishii, Kentaro,Ito, Kentaro,Uchiyama, Susumu,Oda, Masayuki,Iwasaki, Hiroshi,Kim, Seog K,Takahashi, Masayuki Oxford University Press 2018 Nucleic acids research Vol.46 No.5
<P><B>Abstract</B></P><P>Mg<SUP>2+</SUP> ion stimulates the DNA strand exchange reaction catalyzed by RecA, a key step in homologous recombination. To elucidate the molecular mechanisms underlying the role of Mg<SUP>2+</SUP> and the strand exchange reaction itself, we investigated the interaction of RecA with Mg<SUP>2+</SUP> and sought to determine which step of the reaction is affected. Thermal stability, intrinsic fluorescence, and native mass spectrometric analyses of RecA revealed that RecA binds at least two Mg<SUP>2+</SUP> ions with K<SUB>D</SUB> ≈ 2 mM and 5 mM. Deletion of the C-terminal acidic tail of RecA made its thermal stability and fluorescence characteristics insensitive to Mg<SUP>2+</SUP> and similar to those of full-length RecA in the presence of saturating Mg<SUP>2+</SUP>. These observations, together with the results of a molecular dynamics simulation, support the idea that the acidic tail hampers the strand exchange reaction by interacting with other parts of RecA, and that binding of Mg<SUP>2+</SUP> to the tail prevents these interactions and releases RecA from inhibition. We observed that binding of the first Mg<SUP>2+</SUP> stimulated joint molecule formation, whereas binding of the second stimulated progression of the reaction. Thus, RecA is actively involved in the strand exchange step as well as bringing the two DNAs close to each other.</P>
Yanagisawa, Y.,Suetomi, Y.,Piao, R.,Yamagishi, K.,Takao, T.,Hamada, M.,Saito, K.,Ohki, K.,Yamaguchi, T.,Nagaishi, T.,Kitaguchi, H.,Ueda, H.,Shimoyama, J.,Ishii, Y.,Tomita, M.,Maeda, H. The Korea Institute of Applied Superconductivity a 2018 초전도와 저온공학 Vol.20 No.2
The present article briefly overviews the plan for a new project on joint technology for HTS wires/cables and describes the development plan for the world's highest field NMR magnet, which is a major development item in the project. For full-fledged social implementation of superconducting devices, high temperature superconducting (HTS) wire is a key technology since they can be cooled by liquid nitrogen and they can generate a super-high magnetic field of >>24 T at liquid helium temperatures. However, one of the major drawbacks of the HTS wires is their availability only in short lengths of a single piece of wire. This necessitates a number of joints being installed in superconducting devices, resulting in a difficult manufacturing process and a large joint resistance. In Japan, a large-scale project has commenced, including two technical demonstration items: (i) Development of superconducting joints between HTS wires, which are used in the world's highest field 1.3 GHz (30.5 T) NMR magnet in persistent current mode; the joints performance is evaluated based on NMR spectra for proteins. (ii) Development of ultra-low resistive joints between DC superconducting feeder cables for railway systems. The project starts a new initiative of next generation super-high field NMR development as well as that of realization of better superconducting power cables.
High-Power ECRH Experiments in the GAMMA 10 Tandem Mirror
yoshinori Tatematsu,A. Itakura,D. Nagai,H. Higaki,H. Hojo,I. Katanuma,J. Kohagura,K. Nozaki,K. Sakamotoa,K. Ishii,M. Ichimura,M. Yoshikawa,M. Hirata,M. K. Islam,N. Machida,O. Watanabe,T. Imai,T. Numak 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.III
Power-up of gyrotrons was carried out and corresponding launcher systems were designed for plug and central-cell ECRH systems in the GAMMA 10 tandem mirror. Then, a high-power ECRH experiment was started. For the plug ECRH, new 500-kW gyrotrons produced a new record value of the confining potential. For the central-cell ECRH, development of a new antenna system has increased the transmission rate of incident microwave power and focused it onto the machine axis in the resonance surface. As a result, a clear increase of the diamagnetism was observed during the pulse of ECRH.
Physiological Factors Depressing Feed Intake and Saliva Secretion in Goats Fed on Dry Forage
Sunagawa, K.,Ooshiro, T.,Nakamura, N.,Ishii, Y.,Nagamine, I.,Shinjo, A Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.1
Ruminants eating dry forage secrete large volumes of saliva which results in decreased plasma volume (hypovolemia) and the loss of $NaHCO_3$ from the blood. The present research investigated whether or not hypovolemia and the loss of $NaHCO_3$ from the blood in goats brought about by dry forage feeding actually depresses feed intake and saliva secretion, respectively. The present experiment consisted of three treatments (NI, ASI, MI). In the control treatment (NI), a solution was not infused. In the ASI treatment, i.v. infusion of artificial parotid saliva was initiated 1 h before feeding and continued for the entire 2 h feeding period. In the MI treatment, iso-osmotic mannitol solution was infused. The NI treatment showed that hematocrit and plasma total protein concentration were increased due to decreased circulating plasma volume brought about by feeding. In the ASI treatment, the fluid and $NaHCO_3$ that were lost from the blood because of a feeding-induced acceleration of saliva secretion was replenished with an intravenous infusion of artificial parotid saliva. This replenishment lessened the levels of suppression on both feeding and parotid saliva secretion. When only the lost fluid was replenished with an intravenous infusion of iso-osmotic mannitol solution in the MI treatment, the degree of feeding suppression was lessened but the level of saliva secretion suppression was not affected. These results indicate that the marked suppression of feed intake during the initial stages of dry forage feeding was caused by a feeding-induced hypovolemia while the suppression of saliva secretion was brought about by the loss of $NaHCO_3$ from the blood due to increased saliva secretion during the initial stages of feeding.