FSCB phosphorylation in mouse spermatozoa capacitation

( Shun Li Liu ),( Bing Ni ),( Xiang Wei Wang ),( Wen Qian Huo ),( Jun Zhang ),( Zhi Qiang Tian ),( Ze Min Huang ),( Yi Tian ),( Jun Tang ),( Yan Hua Zheng ),( Feng Shuo Jin ),( Yan Feng Li ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.8

It is generally accepted that spermatozoa capacitation is associated with protein kinase A-mediated tyrosine phosphorylation. In our previous study, we identified the fibrous sheath CABYR binding protein (FSCB), which was phosphorylated by PKA. However, the phosphorylation status of FSCB protein during spermatozoa capacitation should be further investigated. To this aim, in this study, we found that phosphorylation of this 270-kDa protein occurred as early as 1 min after mouse spermatozoa capacitation, which increased over time and remained stable after 60 min. Immunoprecipitation assays demonstrated that the tyrosine and Ser/Thr phosphorylation of FSCB occurred during spermatozoa capacitation. The extent of phosphorylation and was closely associated with the PKA activity and spermatozoa motility characteristics. FSCB phosphorylation could be induced by PKA agonist DB-cAMP, but was blocked by PKA antagonist H-89.Therefore, FSCB contributes to spermatozoa capacitation in a tyrosine-phosphorylated format, which may help in further elucidating the molecular mechanism of spermatozoa capacitation. [BMB reports 2011; 44(8): 541-546]

Microfluidic perfusion bioreactor for optimization of microalgal lipid productivity

Paik, Sang-Min,Sim, Sang-Jun,Jeon, Noo Li Elsevier 2017 Bioresource technology Vol.233 No.-

<P><B>Abstract</B></P> <P>Nutrient deprivation in a batch process induces microbes to produce secondary metabolites while drastically constraining cellular growth. A microfluidic continuous perfusion system was designed and tested to culture microalgae, <I>Chlamydomonas reinhardtii</I>, under constant nutrient concentration slightly lower than normal condition. When cultured in 7.5%/7.5% of NH<SUB>4</SUB> <SUP>+</SUP>/PO<SUB>4</SUB> <SUP>2</SUP> <SUP>−</SUP>, <I>C. reinhardtii</I> showed a 2.4-fold increase in TAG production with a 3.5-fold increase in biomass compared to level obtained under an only NH<SUB>4</SUB> <SUP>+</SUP> depleted condition. The microfluidic continuous perfusion bioreactor with steady continuous nutrient flow can be used to optimize conditions for enhancing secondary metabolite production and increasing microbial biomass.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Microfluidic perfusion bioreactor is designed for secondary metabolite production. </LI> <LI> Continuous supply of low concentration of nutrient permits microbes to grow at a minimum rate. </LI> <LI> Low nutrient allows same level of metabolite production compared to depleted condition. </LI> <LI> Stable feed of low level of nutrient is an effective way to enhance total TAG yield. </LI> </UL> </P>

Vibration-induced stress priming during seed culture increases microalgal biomass in high shear field-cultivation

Paik, Sang-Min,Jin, EonSeon,Sim, Sang Jun,Jeon, Noo Li Elsevier Applied Science 2018 Bioresource technology Vol.254 No.-

<P><B>Abstract</B></P> <P>Vibrational wave treatment has been used to increase proliferation of microalgae. When directly applied at large scale, however, turbulence can offset positive effects of vibration on microalgae proliferation. Moreover, severe hydrodynamic shear fields in the bioreactor decrease cell viability that detrimentally influence maximum yieldable biomass. In this study, vibration pretreatment (between 10–30 Hz and 0.15–0.45 G) was used to prime the cells for enhanced biomass. When exposed to 10 Hz at 0.15 G for 72 h and inoculated in baffled flasks of large shear fields (0.292 Pa for the average wall shear force (aveWSF) and 184 s<SUP>−1</SUP> for the average shear strain rate (aveSSR)), microalgae showed 27% increase in biomass as well as 39% increase in corresponding amount of heterologous protein (i.e. GFP-3HA). Our results show that stress primed microalgae with vibrations can lead to improved proliferation that results in increased biomass production at industrial scale bioprocesses.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Vibration put microalgal cells into a primed state where to endure severe stresses. </LI> <LI> Primed microalgal cells grew more in harsh shear field-cultivation. </LI> <LI> Stress-priming can contributes to biomass increase and efficient large-scale bioprocess. </LI> <LI> TRP11 was cross-activated linking stresses from mild vibration and harsh shaking. </LI> </UL> </P>

Biochar-supported nZVI (nZVI/BC) for contaminant removal from soil and water: A critical review

Wang, Shengsen,Zhao, Mingyue,Zhou, Min,Li, Yuncong C.,Wang, Jun,Gao, Bin,Sato, Shinjiro,Feng, Ke,Yin, Weiqin,Igalavithana, Avanthi Deshani,Oleszczuk, Patryk,Wang, Xiaozhi,Ok, Yong Sik Elsevier 2019 Journal of hazardous materials Vol.373 No.-

<P><B>Abstract</B></P> <P>The promising characteristics of nanoscale zero-valent iron (nZVI) have not been fully exploited owing to intrinsic limitations. Carbon-enriched biochar (BC) has been widely used to overcome the limitations of nZVI and improve its reaction with environmental pollutants. This work reviews the preparation of nZVI/BC nanocomposites; the effects of BC as a supporting matrix on the nZVI crystallite size, dispersion, and oxidation and electron transfer capacity; and its interaction mechanisms with contaminants. The literature review suggests that the properties and preparation conditions of BC (e.g., pore structure, functional groups, feedstock composition, and pyrogenic temperature) play important roles in the manipulation of nZVI properties. This review discusses the interactions of nZVI/BC composites with heavy metals, nitrates, and organic compounds in soil and water. Overall, BC contributes to the removal of contaminants because it can attenuate contaminants on the surface of nZVI/BC; it also enhances electron transfer from nZVI to target contaminants owing to its good electrical conductivity and improves the crystallite size and dispersion of nZVI. This review is intended to provide insights into methods of optimizing nZVI/BC synthesis and maximizing the efficiency of nZVI in environmental cleanup.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Aggregation and passivation of nZVI can be alleviated by surfactants and doping methods. </LI> <LI> BC hinders corrosion and improves the dispersion and electron transfer of nZVI. </LI> <LI> Properties of nZVI depend on those of the BC, feedstock and pyrogenic temperature. </LI> <LI> BC enhances electron transfer from nZVI to the contaminants due to the presence of quinone and graphene moieties. </LI> <LI> nZVI/BC shows strong ability to remove HMs, nitrates, and organic contaminants in soil and water. </LI> </UL> </P>

Silencing of long noncoding RNA HOXA11-AS inhibits the Wnt signaling pathway via the upregulation of HOXA11 and thereby inhibits the proliferation, invasion, and self-renewal of hepatocellular carcinoma stem cells

Jun-Cheng Guo,Yi-Jun Yang,Jin-Fang Zheng,Jian-Quan Zhang,Min Guo,Xiang Yang,Xiang-Ling Jiang,Li Xiang,You Li,Huang Ping,Liu Zhuo 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Hepatocellular carcinoma (HCC) is a major cause of cancer-related deaths, but its molecular mechanisms are not yet well characterized. Long noncoding RNAs (lncRNAs) play crucial roles in tumorigenesis, including that of HCC. However, the role of homeobox A11 antisense (HOXA11-AS) in determining HCC stem cell characteristics remains to be explained; hence, this study aimed to investigate the effects of HOXA11-AS on HCC stem cell characteristics. Initially, the expression patterns of HOXA11-AS and HOXA11 in HCC tissues, cells, and stem cells were determined. HCC stem cells, successfully sorted from Hep3B and Huh7 cells, were transfected with short hairpin or overexpression plasmids for HOXA11-AS or HOXA11 overexpression and depletion, with an aim to study the influences of these mediators on the self-renewal, proliferation, migration, and tumorigenicity of HCC stem cells in vivo. Additionally, the potential relationship and the regulatory mechanisms that link HOXA11-AS, HOXA11, and the Wnt signaling pathway were explored through treatment with Dickkopf-1 (a Wnt signaling pathway inhibitor). HCC stem cells showed high expression of HOXA11-AS and low expression of HOXA11. Both HOXA11-AS silencing and HOXA11 overexpression suppressed the self-renewal, proliferation, migration, and tumorigenicity of HCC stem cells in vivo, as evidenced by the decreased expression of cancer stem cell surface markers (CD133 and CD44) and stemness-related transcription factors (Nanog, Sox2, and Oct4). Moreover, silencing HOXA11-AS inactivated the Wnt signaling pathway by decreasing the methylation level of the HOXA11 promoter, thereby inhibiting HCC stem cell characteristics. Collectively, this study suggested that HOXA11-AS silencing exerts an antitumor effect, suppressing HCC development via Wnt signaling pathway inactivation by decreasing the methylation level of the HOXA11 promoter.

Suspended membrane bioreactor with extracellular polymeric substances as reserve carbon source for low carbon to nitrogen ratio wastewater: Performance and microbial community composition

Min Li,Jun Feng Su,Yifei Li,Amjad Ali,Shu Yang,Shuai Zhang 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.9

A suspended membrane bioreactor (SMBR) was employed to treat low carbon to nitrogen (C/N) ratio wastewater. The suspended membranes amplified by Comamonas sp. YSF15 were collected to develop the SMBR, which showed excellent performance for nitrate (NO3 ) removal. The maximum nitrate removal efficiency of 95.22% was obtained at an initial nitrate concentration of 20mg L1, HRT of 6 h, and C/N=2.5 : 1 (molar ratio). In addition, the polysaccharide (PS) and protein (PN) compositions of the soluble microbial products (SMP), extracellular polymeric substances (EPS) and the Fourier transform infrared (FTIR) spectra indicated that the suspended membrane utilized its SMP and EPS as reserve C source to achieve higher denitrification performance under the low C/N ratio. With the increase of pH, the generated SMP and EPS in SMBR continued to increase. The increase in PS content was significantly greater than that of PN, thus forming a suspended membrane with a certain mechanical strength. Highthroughput sequencing data indicated that Comamonas sp. YSF15 played a key role in effective nitrate removal by SMBR. It can adapt to a nutrient-deficient environment, especially low (C/N) ratio, and greatly increase in the late operation of the reactor.

Enhancement of light absorption using high-<i>k</i> dielectric in localized surface plasmon resonance for silicon-based thin film solar cells

Li, Hua-Min,Zhang, Gang,Yang, Cheng,Lee, Dae-Yeong,Lim, Yeong-Dae,Shen, Tian-Zi,Yoo, Won Jong,Park, Young Jun,Kim, Hyunjin,Nam Cha, Seung,Kim, Jong Min American Institute of Physics 2011 JOURNAL OF APPLIED PHYSICS - Vol.109 No.9

High-k ZrO2 Enhanced Localized Surface Plasmon Resonance for Application to Thin Film Silicon Solar Cells

Hua-Min Li,Gang Zang,Cheng Yang,Yeong-Dae Lim,Tian-Zi Shen,Won Jong Yoo,Young Jun Park,Jong Min Lim 한국진공학회 2010 한국진공학회 학술발표회초록집 Vol.38 No.-

Comprehensive Bioinformation Analysis of the MRNA Profile of Fascin Knockdown in Esophageal Squamous Cell Carcinoma

Wu, Bing-Li,Luo, Lie-Wei,Li, Chun-Quan,Xie, Jian-Jun,Du, Ze-Peng,Wu, Jian-Yi,Zhang, Pi-Xian,Xu, Li-Yan,Li, En-Min Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background: Fascin, an actin-bundling protein forming actin bundles including filopodia and stress fibers, is overexpressed in multiple human epithelial cancers including esophageal squamous cell carcinoma (ESCC). Previously we conducted a microarray experiment to analyze fascin knockdown by RNAi in ESCC. Method: In this study, the differentially expressed genes from mRNA expression profilomg of fascin knockdown were analyzed by multiple bioinformatics methods for a comprehensive understanding of the role of fascin. Results: Gene Ontology enrichment found terms associated with cytoskeleton organization, including cell adhesion, actin filament binding and actin cytoskeleton, which might be related to fascin function. Except GO categories, the differentially expressed genes were annotated by 45 functional categories from the Functional Annotation Chart of DAVID. Subpathway analysis showed thirty-nine pathways were disturbed by the differentially expressed genes, providing more detailed information than traditional pathway enrichment analysis. Two subpathways derivated from regulation of the actin cytoskeleton were shown. Promoter analysis results indicated distinguishing sequence patterns and transcription factors in response to the co-expression of downregulated or upregulated differentially expressed genes. MNB1A, c-ETS, GATA2 and Prrx2 potentially regulate the transcription of the downregulated gene set, while Arnt-Ahr, ZNF42, Ubx and TCF11-MafG might co-regulate the upregulated genes. Conclusions: This multiple bioinformatic analysis helps provide a comprehensive understanding of the roles of fascin after its knockdown in ESCC.

Chemokine Signaling Pathway Involved in CCL2 Expression in Patients with Rheumatoid Arthritis

Lin Zhang,Changyi Li,Min Yu,Jiayin Deng,Xing Lv,Jun Liu,Yu Xiao,Wenjie Yang,Yuru Zhang 연세대학교의과대학 2015 Yonsei medical journal Vol.56 No.4

Purpose: Rheumatoid arthritis (RA) is an inflammatory joint disorder, the progressionof which leads to the destruction of cartilage and bone. Chemokines are involvedin RA pathogenesis. In this study, we investigated the chemokine signaling pathway associated with CCL2 in peripheral blood (PB) and synovial tissues (ST) of RA patients based on our previous work about chemokine signaling pathway involvedin the activation of CCL2 production in collagen-induced arthritis rat ST. Materials and Methods: Total RNA was isolated from PB leukocytes and synoviumof the knee joint in both RA patients and control populations. Real-time polymerasechain reaction was used to determine CCL4, CCR5, c-Jun, c-Fos, and CCL2 expressions. Serum level of CCL2 was assessed by enzyme-linked immunosorbent assay, and the production of CCL2 in ST was analyzed immunohistochemically. Results: The expressions of CCL4, CCR5, c-Jun, c-Fos, and CCL2 messenger RNA in RA patients were significantly higher than those in healthy controls, both in ST and on PB leukocyte. Serum CCL2 levels were elevated in RA patients. Histological examination of rheumatoid joints revealed extensive CCL2 expression in RA ST. Conclusion: CCL2, CCL4, c-Jun, c-Fos, and CCR5 may play an important role in the recruitment of PB leukocytes into the RA joints. These data provide evidence that the chemokine signaling pathway is involved in CCL2 expression in RA patient tissues, which may contribute to chronic inflammation associated with RA. Targetingthis signaling pathway may provide a novel therapeutic avenue in RA.