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Jorge A. Gonzalez-y-Merchand,Ruben Zaragoza-Contreras,Rosalina Guadarrama-Medina,Addy C. Helguera-Repetto,Sandra Rivera-Gutierrez,Jorge F. Cerna-Cortes,Leopoldo Santos-Argumedo,Robert A. Cox 한국미생물학회 2012 The journal of microbiology Vol.50 No.3
The study of the in vitro cell growth of mycobacteria still remains a fastidious, difficult, and time-consuming procedure. In addition, assessing mycobacterial growth in the laboratory is often complicated by cell aggregation and slow growth-rate. We now report that the use of a stainless steel spring in the culture led to an absence of large cell clumps, to a decrease of dead cells in the exponential phase and to growth of a more homogeneous population of large cells. We also report that flow cytometry is a rapid, simple and reliable approach to monitor mycobacterial cell growth and viability. Here, we monitored Mycobacterium smegmatis cellular growth by optical density, dry cell mass, and colony forming units; in addition, viability, cell size and granularity profiles were analyzed by flow cytometry, and cell morphology by electron microscopy. Cultures monitored by flow cytometry may lead to a better understanding of the physiology of mycobacteria. Moreover, this methodology may aid in characterizing the cell growth of other fastidious species of microorganisms.
Gonzalez-Cabrera Jaime,Moreno-Carrillo Gabriel,Lopez-Juarez Alejandro A.,Anguiano-Amezcua Juan A.,Sanchez-Gonzalez Jorge A.,Mendoza-Ceballos Mario Y.,Arredondo-Bernal Hugo C. 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2
Two colonies of the parasitoid Trichopria drosophilae (Perkins) (Hymenoptera: Diapriidae) are reproduced using a mass rearing approach in Mexico. The first colony uses the worldwide commercial host Drosophila melanogaster Meigen (Diptera: Drosophilidae) reared on an artificial diet (=T. drosophilae-Dm), while the second colony uses D. suzukii Matsumura reared on fresh banana (=T. drosophilae-Ds). Since theoretically the type of colony host affects parasitoid field performance, we conducted concatenated laboratory and field studies of both parasitoid lines. In the laboratory trials, T. drosophilae-Ds exhibited greater longevity, larger adult size, and higher fecundity than T. drosophilae-Dm. Both lines of parasitoids oviposited indistinctly in three drosophilid species commonly found in the berry zones of Colima: D. melanogaster, D. suzukii, and Zaprionus indianus Gupta. In the field trials, T. drosophilae-Ds had a larger population size and provided 2.4-fold higher pest reduction compared to T. drosophilae-Dm. Under the particular conditions of these field trials (berry fields separate from each other ≤ 3 km, and intensive agronomic management), these results demonstrate that T. drosophilae-Ds can significantly contribute more to the integrated management of D. suzukii than when reared on the commercial host D. melanogaster.
홍남기,이용호,Kenichi Tsujita,Jorge A. Gonzalez,Christopher M. Kramer,Tomas Kovarnik,George N. Kouvelos,Hiromichi Suzuki,한경도,이찬주,박성하,이병완,차봉수,강은석 대한내분비학회 2018 Endocrinology and metabolism Vol.33 No.2
Background: Ezetimibe-statin combination therapy has been found to reduce low density lipoprotein cholesterol levels and the risk of major adverse cardiovascular events (MACEs) in large trials. We sought to examine the differential effect of ezetimibe on MACEs when added to statins according to the presence of diabetes. Methods: Randomized clinical trials with a sample size of at least 50 participants and at least 24 weeks of follow-up that compared ezetimibe-statin combination therapy with a statin- or placebo-controlled arm and reported at least one MACE, stratified by diabetes status, were included in the meta-analysis and meta-regression. Results: A total of seven trials with 28,191 enrolled patients (mean age, 63.6 years; 75.1% men; 7,298 with diabetes [25.9%]; mean follow-up, 5 years) were analysed. MACEs stratified by diabetes were obtained from the published data (two trials) or through direct contact (five trials). No significant heterogeneity was observed among studies (I2=14.7%, P=0.293). Ezetimibe was associated with a greater reduction of MACE risk in subjects with diabetes than in those without diabetes (pooled relative risk, 0.84 vs. 0.93; Pheterogeneity=0.012). In the meta-regression analysis, the presence of diabetes was associated with a greater reduction of MACE risk when ezetimibe was added to statins (β=0.87, P=0.038). Conclusion: Ezetimibe-statin combination therapy was associated with greater cardiovascular benefits in patients with diabetes than in those without diabetes. Our findings suggest that ezetimibe-statin combination therapy might be a useful strategy in patients with diabetes at a residual risk of MACEs.
Cosmic CARNage I: on the calibration of galaxy formation models
Knebe, Alexander,Pearce, Frazer R,Gonzalez-Perez, Violeta,Thomas, Peter A,Benson, Andrew,Asquith, Rachel,Blaizot, Jeremy,Bower, Richard,Carretero, Jorge,Castander, Francisco J,Cattaneo, Andrea,Cora, S Oxford University Press 2018 MONTHLY NOTICES- ROYAL ASTRONOMICAL SOCIETY Vol.475 No.3
<P>We present a comparison of nine galaxy formation models, eight semi-analytical, and one halo occupation distribution model, run on the same underlying cold dark matter simulation (cosmological box of comoving width 125h(-1) Mpc, with a dark-matter particle mass of 1.24 x 10(9) h(-1)M(circle dot)) and the same merger trees. While their free parameters have been calibrated to the same observational data sets using two approaches, they nevertheless retain some 'memory' of any previous calibration that served as the starting point (especially for the manually tuned models). For the first calibration, models reproduce the observed z = 0 galaxy stellar mass function (SMF) within 3 sigma The second calibration extended the observational data to include the z = 2 SMF alongside the z similar to 0 star formation rate function, cold gas mass, and the black hole bulge mass relation. Encapsulating the observed evolution of the SMF from z = 2 to 0 is found to be very hard within the context of the physics currently included in the models. We finally use our calibrated models to study the evolution of the stellar-to-halo mass (SHM) ratio. For all models, we find that the peak value of the SHM relation decreases with redshift. However, the trends seen for the evolution of the peak position as well as the mean scatter in the SHM relation are rather weak and strongly model dependent. Both the calibration data sets and model results are publicly available.</P>
Self-assembly study of type I collagen extracted from male Wistar Hannover rat tail tendons
Jeimmy González-Masís,Jorge M. Cubero-Sesin,Simón Guerrero,Sara González-Camacho,Yendry Regina Corrales-Ureña,Carlos Redondo-Gómez,José Roberto Vega-Baudrit,Rodolfo J. Gonzalez-Paz 한국생체재료학회 2020 생체재료학회지 Vol.24 No.4
Background: Collagen, the most abundant protein in the animal kingdom, represents a promising biomaterial for regenerative medicine applications due to its structural diversity and self-assembling complexity. Despite collagen’s widely known structural and functional features, the thermodynamics behind its fibrillogenic self-assembling process is still to be fully understood. In this work we report on a series of spectroscopic, mechanical, morphological and thermodynamic characterizations of high purity type I collagen (with a D-pattern of 65 nm) extracted from Wistar Hannover rat tail. Our herein reported results can be of help to elucidate differences in selfassembly states of proteins using ITC to improve the design of energy responsive and dynamic materials for applications in tissue engineering and regenerative medicine. Methods: Herein we report the systematic study on the self-assembling fibrillogenesis mechanism of type I collagen, we provide morphological and thermodynamic evidence associated to different self-assembly events using ITC titrations. We provide thorough characterization of the effect of pH, effect of salts and protein conformation on self-assembled collagen samples via several complementary biophysical techniques, including circular dichroism (CD), Fourier Transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), atomic force microscopy (AFM), scanning electron microscopy (SEM), dynamic mechanical thermal analysis (DMTA) and thermogravimetric analysis (TGA). Results: Emphasis was made on the use of isothermal titration calorimetry (ITC) for the thermodynamic monitoring of fibrillogenesis stages of the protein. An overall self-assembly enthalpy value of 3.27 ± 0.85 J/mol was found. Different stages of the self-assembly mechanism were identified, initial stages take place at pH values lower than the protein isoelectric point (pI), however, higher energy release events were recorded at collagen’s pI. Denatured collagen employed as a control exhibited higher energy absorption at its pI, suggesting different energy exchange mechanisms as a consequence of different aggregation routes.