발효조를 이용한 Monascus anka의 적색소와 황색소의 생산

강성국,임종환,정순택,김선재 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

Monascus anka를 이용하여 천연식용색소를 대량생산하기 위한 방법을 개발하기 위하여 발효조를 이용하여 적색소와 황색소의 색소생산의 최적 배양 조건과 생산된 색소의 색깔 특성을 조사하였다. Monascus anka 적색소와 황색소를 생산하였는데, 이들 색소는 균체내색소와 균체외 색소로 구분되었다. 균체의 적색소(ERP)는 494nm에서, 균체외 황색소(EYP)는 380nm에서, 균체내 적색소(IRP)는 506nm에서 그리고 균체내 황색소(IYP)는 388nm에서 최대 흡광도를 나타냈다. 적색소와 황색소 생산, 색조의 특성 및 균체증식력을 배양 온도, pH, 쌀가루 농도, peptone 농도, magnesium sulfate 농도, 통기량 및 교반속도에 대하여 조사한 결과 적색소 생산력은 30℃, 초기 pH 6.0, 쌀가루 농도 3~5%, peptone 농도 0.05%, magnesium sulfate 농도 0.25%, 통기량 0.1vvm 교반속도 300rpm의 조건에서 최대치를 보였으며, 이때 ERP, EYP, IRP 및 IYP는 각각 A_494nm 0.84, A_380 nm 0.71, A_506 nm 1.18, A_388 nm 1.10, L값은 각각 29.40, 6.44, 34,98, 8.94, a값은 각각 5.76, -1.08, 11.83, -1.55, b값은 각각 18-19, 3.41 27.26, 6.16 그리고 균체량은 7.4 g/l을 보였다. 또한 황색소 생산력은 온도의 경우 적색소 생산을 위한 최적온도 보다 높은 35℃ 부근에서 우수하였으며 초기 pH와 쌀가루 농도는 적색소 생산을 위한 최적조건과 같았고, 질소원과 무기염의 농도가 높을수록 그리고 통기량이 많을수록 황색소 생산력이 우수하였다. 적색소와 황색소 생산을 위한 발효조의 교반속도는 100~300 rpm이 적합하였다. Production of Red and Yellow Pigments from Monascus anda in a Jar Fermenter, Seong-Gook Kang, Jong-Whan Rhim, Soon-Teck Jung^* and Sun-Jae Kim. Department of Food Engineering. Mokpo National University Chonnam, 534-729, Korea - In order to develop the method for mass production of natural food colorant from Monascus anka, optimum cultivation conditions for producing red and yellow pigments by cultivating the mold in a jar fermenter and their color characteristics were investigated. The mold produced red and yellow pigments both intracellularly and extracellularly. These pigments showed unique light absorption characteristics with maximum absorption of 494, 380, 506, and 388 nm for extracellular red pigment (ERP), extracellular yellow pigment (EYP), intracellular red pigment (IRP), and intracellular yellow pigment (IYP), respectively. Optimum conditions for producing red pigments were found to be temperature 30℃, initial pH 6.0, rice powder 3~5%, peptone 0.05%, magnesium sulfate 0.25%, aeration rate 0.1vvm. Optimum temperature for producing yellow pigments was around 35℃ which is higher than that of producing red pigments. The initial pH rice powder concentration for producing yellow pigments were the same as those of producing red pigments. The higher concentration of nitrogen source and inorganic salt, aeration rate, the more the yellow pigments were produced. Them optimum agitation speed was 100~300 rpm for pigment production.

400 계열 Stainless Steel Alloy의 소결을 이용한 ABS Sensor Ring 개발

林鍾國 충주대 2001 한국교통대학교 논문집 Vol.36 No.1

ABS sensor ring has been manufactured by P/M method using 400 series ferrite stainless steel.The results are following. It is supposedly sufficient to use for control computer due to good experimental results of magnetic characteristics, Compared with sensor ring made by iron, 400 series ferrite stainless steel has shown a good corrosion resistance without an addition surface treatment.Thus the decreasing production process has been obtained. As a result of hardness and tensile test, 400 series ferrite stainless steel shown a good endurance when it is combined to C/V joint, and has a good hardness properties endurable to sand and pebble impact.

400계열 Stainless Steel 분말의 소결에 따른 자기적 특성

林鍾國 忠州大學校 1998 한국교통대학교 논문집 Vol.33 No.1

Recent trends that the sintered powder has applied to the machinery parts with magnetic characteristic have accelerated by the development of sintering process of the powder with the high density. The study is carried out to find the variation of the magnetic properties in 4 types of stainless steel powder. The excellent magnetic properties can be obtained from 400 series ferrite stainless steels, despide of relatively low sintering density of 6.8g/㎤, 434L satisfied the magnetic properties to be applied to ABS sensor ring in automotive, density and max permeability and high coercive force. The magnetic property of 410L was affected by a precipitation of carbide which was not shown in 434L series. However, several particles such as Cr₂O₃and Cr₂N were precipitated in alloy, 434LD2, of which has little effect on the magnetic properties. An increase of C content may result from a carburization by the remaining binder and the environment. In case of using cooling medium as nitrogen, the gas is required to inject at temperature lower than 500℃ to avoid nitration.

벌징현상을 고려한 항공기 동체의 균열 성장 해석

林鍾國 충주대 2000 한국교통대학교 논문집 Vol.35 No.1

The bulging is phenomena observed near a longitudinal crack in cylindrical structure.The bulging phenomena increase the stress intensity factor values and the constant term of the longitudinal stress near crack tip, which is the T stress.The T stress affects the crass path instability.When the T stress is positive, the crack can grow perpendicular to the original crack path.And this phenomena is called flapping.The paper suggests a criterion which can determine the flapping condition.

충주대학교 교육과정의 필수·선택교과목 폐지에 대한 小考

임종국 충주대학교 학생생활연구소 2002 學生生活硏究 Vol.9 No.-

소결금속을 이용한 LPG용기용 안전 밸브의 개발

임종국 忠州大學校 2005 한국교통대학교 논문집 Vol.40 No.2

This study is a research of safety valve development for LP gas cylinder which use sintering metal. Researcher wiches to apply technology of sintering metal for safety valve development and do gas flow control. The basis of this study is most suitable fluid examination that to reduce gas accident. This research concluded following results. 1. When press pin length is 42mm to 45mm powder quantity is 0.25g, in case of press pin length 36mm to 42mm powder quantity is 0.2g, displayed fluid optimization. 2. When press pin length is 39mm and powder quantity is 0.25g, press pressure displayed fluid optimization at all niterests from 1.2 tons/㎠ to 2 tons/㎠. 3. when apparent density is about 5.0g/㎤ to 4.5㎤, fluid optimization becomes.

LP가스 용기용 안전밸브개발에 관한 연구

임종국 忠州大學校 2004 한국교통대학교 논문집 Vol.39 No.1

In recent, the gas accident increase according to the use of gas increased. This study introduced to safety valve and regulator over-flow cut-off valve for LPG cylinder. Its prevent gas accident from careless use. Many studies had done in Japan and Korea, but not used yet. This report suggest a substitute for practical use.

FAS 활성 저해물질을 함유한 식품의 섭취에 따른 여성의 비만관련 체구성요인 및 배변만족도의 변화

임용택,김재등,이충영,김종길 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

The purpose of this study was to investigate the effect of food including component inhibiting activity of FAS supplementation and regulatory exercise on body composition concerning obesity and defecation satisfaction in women. Twenty healthy but on mild obesity women were participated as a subject. Body composition and defecation satisfaction were measured before and after 3 and 6 weeks' supplementation and exercise. The results of this study were as follows : First, body composition concerning obesity such as body weight(p= .0001), %body fat(p= .0001) and WHR(p= .0001) after 6weeks' food supplementation and exercise was decreased significantly. Second, defecation satisfaction(p= .0001) after 6weeks' food supplementation and exercise was increased significantly. This results suggested that food including component inhibiting activity of FAS supplementation and regulatory exercse should be recommended as a effective method for cure and prevention obesity and constipation.

Ferritic Stainless Steel의 소결조건에 따른 자기적 특성

林鍾國 충주대 산업과학기술연구소 1999 産業科學論文集 Vol.7 No.1

It is well known for ferritic stainless steel powder to applicate a sensor ring in anti-lock brake system of automobile, several studies, because of its excellent magnetic properties. This study was carried out to investigate the magnetic properties such as the maximum magnetic induction, coercivity and maximum permeability of the materials with functions of sintering density, time and temperature, and concluded as follows; 1.Both maximum magnetic induction value of 4700 Gauss and permeability of 200 were obtained at the maximum sintering density of 6.89g/cm3. Here, the properties showed a linear increasement with increasing the sintering density. 2.Coercivity sharply increased with incresing the sintering density and reached to 7.6 Oe at the maximum sintering density of 6.89g/cm3. 3.With increasing the sintering density, the temperature from 1140℃ to 1250℃ and the time from 30min to 60min, the maximum magnetic induction rate, coercivity and maximum permeability were increased simultaneously.

기계적 합금화법에 의한 Al-Fe-Cr합금의 크��거동

임종국 忠州大學校 1999 한국교통대학교 논문집 Vol.34 No.1

기계적 합금화법으로 제조된 Al-Fe-Cr 합금의 크맆거동을 40 ∼ 115Mpa 응력범위와, 300 ∼ 441℃(0.53 ∼ 0.66Tm) 온도 범위에서 조사하였다. 이 계열의 합금은 비행기 및 자동차의 구조용재료 혹은 엔진용 부품에 많이 사용되고 있으며, 재료의 사용이 주로 고온에서 이루어지므로 안전성을 확보하기 위해서는 크맆실험이 특히 중요하다. 이 합금의 크맆 실험 결과 응력지수와 크맆 활성화에너지가 높았으며 실험 응력과 온도에 크게 좌우되었다. 크맆 응력이 조대화에 강하게 영향 미치는 것으로 보이기 때문에 모든 크맆 시편의 분산입자의 조대화 율은 등온 소둔 시편 보다도 더 빠르게 나타났다. 분산상과 연결된 전위는 고 응력, 저온의 크맆 시편에서 더욱 자주 관찰되었다. Power law creep 에서늬 크맆 변형 속도는 문턱응력과 전위분리기구를 포함하는 Sherby 와 Rosler/Arzt 식으로 예견되는 것과 일치함을 발견하였다. 이 합금에서 분산 상은 void 생성 원으로 작용하였으며 소위 입계파괴인 입자내의 연성파괴의 원인이 되었다. 생성된 void 는 성장하여 Al 기지내의 분산상과 분리되고, 슬립에 의해 결정립계에 직접되어 결국 입계파괴가 일어났다. 그러므로 이들 분산상이 Al13Fe₄, Al13Cr₂ 및 Al₂O₃의 형성에 의해 파괴 기구의 중요한 역할을 함이 입증되었다. The creep behavior of a rapidly solidified and consolidated Al-9.45wt% Fe-4.45wt% Cr alloy were investigated in the stress range 40 to 115 MPa and temperature range 300(0.53Tm) to 441℃(0.66Tm). It is of use to available aerospace and automobile industries for the improved performance of materials used at high temperature. Because Al alloys with improved creep resistance offer the potential for lower weight and reduced costs in aerospace and automobile components (e. g.,structural members and engine parts) through the replacement of heavier and more costly materials, the safety in use at high temperature is good. The alloy is characterized by high stress exponents and activation energies for creep, which are greatly dependent on the stress and temperature. Because the creep stress is seen to cause a strongly significant enhancement of coarsening, the coarsening rate of the dispersed particles in all crept specimens is faster than that in isothermally annealed specimens. Dislocations connecting dispersoids are observed more frequently in crept specimens with higher stress and lower temperature. The creep strain rates in the power law creep regime were found to be predicted much better by the Sherby and Rosler /Arzt equation with the inclusing of a threshold stress and dislocation detachment mechanism. The dispersoids in this alloy were acting a source of void nucleation that finally leaded to ductile fracture within the grain so called intergranular. Each void was initiated, grown and failed at the dispersoids in the aluminium matrix. Grain boundary accommodation of the slip produced, which result in initiation of the void and then final transgranular fracture. Therefore, it was confirmed that these dispersoids played an important role in the fracture mechanism by the formation of Al13Fe₄,Al13Cr₂and Al₂O₃.