Joint toxicity of methoxyfenozide and lufenuron on larvae of Spodoptera exigua Hübner (Lepidoptera: Noctuidae)

Jixiang Chen,Weili Jiang,Hongyan Hu,Xiaoyan Ma,Qian Li,Xianpeng Song,Xiangliang Ren,Yan Ma 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3

The joint action and sublethal effects of methoxyfenozide and lufenuron were measured against Spodoptera exigua. Methoxyfenozide and lufenuron exhibited optimum synergistic toxicity on S. exigua at a mass ratio of 4:6, and the co-toxicity coefficient (CTC) was 165.705. Third instars larvae of S. exigua were treated with methoxyfenozide (LC 15 = 21.004 ng/cm 2 ), lufenuron (LC 15 = 27.134 ng/cm 2 ), or a mixture of methoxyfenozide and lufenuron (MML, LC 15 = 16.503 ng/cm 2 ) through feeding for 72 h. Ingestion of MML by larvae significantly inhibited larval and pupal weights and pupation rate, and prolonged the larval and pupal development of S. exigua compared to individual treatment ofmethoxyfenozide or lufenuron. Both methoxyfenozide and MML treatments significantly decreased the fertility of female S. exigua. No significant changes were observed in case of adult emergence and egg hatching for different treatments. The MML-treated S. exigua exhibited significantly lower activities of polyphenol oxidase (PPO) and cytochrome P450 (CYP450) than those in S. exigua treated separately with methoxyfenozide or lufenuron. Finally, methoxyfenozide, lufenuron, and MML treatments decreased chitinase, acetylcholinesterase (AChE), carboxylesterase (CarE), and glutathione-s-transferase (GST) activities in S. exigua.

Detection of duck hepatitis virus serotype1 by biosensor based on imaging ellipsometry

Chenghong Huang,Jixiang Li,Yu Tang,Yanyan Chen,Gang Jin 한국물리학회 2011 Current Applied Physics Vol.11 No.3

A novel method for the detection of duck hepatitis virus serotype1 (DHV1) using a biosensor based on imaging ellipsometry (IE) is developed. Polyclonal antibodies against DHV1 were immobilized on silicon wafers through protein A and used to capture virus particles in sample. Signals on silicon surface were acquired directly in grayscale format on an imaging ellipsometry image. This study demonstrated that the assay is rapid and accurate for detection of duck hepatitis virus. Compared with traditional methods,this technique not only has high sensitivity as low as 8 × 10^-9.5 LD_50/ml, but can also simultaneously perform multiple serotype identification of DHV1, DHV1-g and DHV1-s. These results suggest that this biosensor might be a practicable diagnostic tool for duck hepatitis virus detection.

miR-148b-3p, miR-190b, and miR-429 Regulate Cell Progression and Act as Potential Biomarkers for Breast Cancer

Wenzhu Dai,Jixiang He,Ling Zheng,Mingyu Bi,Fei Hu,Minju Chen,Heng Niu,Jingyu Yang,Ying Luo,Wenru Tang,Miaomiao Sheng 한국유방암학회 2019 Journal of breast cancer Vol.22 No.2

Purpose: Breast cancer is the most frequently diagnosed malignancy in women worldwide. MicroRNAs (miRNAs) are thought to serve as potential biomarkers in various cancers, including breast cancer. Methods: We evaluated the miRNA expression profiles in 1,083 breast cancer samples and 104 normal breast tissues from The Cancer Genome Atlas database. We used the edgeR package of R software to analyze the differentially expressed miRNAs in normal and cancer tissues, and screened survival-related miRNAs by Kaplan-Meier analysis. A receiver operating characteristic curve was generated to evaluate the accuracy of these miRNAs as molecular markers for breast cancer diagnosis. Furthermore, the functional role of these miRNAs was verified using cell experiments. Targets of candidate miRNAs were predicted using 9 online databases, and Gene Ontology (GO) functional annotation and pathway analyses were conducted using Database for Annotation, Visualization and Integrated Discovery online tool. Results: A total of 68 miRNAs showed significantly different expression patterns between the groups (p < 0.001), and 13 of these miRNAs were significantly associated with poor survival (p < 0.05). Three miRNAs with high specificity and sensitivity, namely, miR-148b-3p, miR-190b, and miR-429, were selected. In vitro experiments showed that the overexpression of these 3 miRNAs significantly promoted the proliferation and migration of MDA-MB-468 and T47D cells and reduced the apoptosis of T47D cells. GO and pathway enrichment analyses revealed that the targets of these dysregulated miRNAs were involved in many critical cancer-related biological processes and pathways. Conclusion: The miR-148b-3p, miR-190b, and miR-429 may serve as potential diagnostic and prognostic markers for breast cancer. This study demonstrated the roles of these 3 miRNAs in the initiation and progression of breast cancer.

Preparation of Silica Nanoparticles via Two-Step Process Utilizing Mixed Chlorosilane Residues

Su, Yonghong,Xu, Bugang,Cai, Jixiang,Chen, Liang,Huang, Bing The Korean Ceramic Society 2018 한국세라믹학회지 Vol.55 No.6

We propose an economic and facile method for the preparation of silica nanoparticles through a two-step process utilizing chlorosilane residues. Mixed chlorosilane residue was alcoholized with absolute ethanol as a first step to form tetraethoxysilane (TEOS). The TEOS was then utilized as a silicon source to synthesize silica nanoparticles in a sol-gel method. The alcoholysis process was designed and optimized utilizing the Taguchi experimental design method and the yield of TEOS was as high as 82.2% under optimal synthetic conditions. Similarly, the Taguchi method was also utilized to study the effects of synthesis factors on the particle size of silica nanoparticles. The results of statistical analysis indicate that the concentration of ammonia has a greater influence on particle size compared to the mass fractions of TEOS and polyethylene glycol (4.6% and 9.7%). The purity of the silica particles synthesized in our experiments is high, but the specific surface area and pore volume are small.

Transcriptome Sequencing Reveals the Potential Mechanisms of Modified Electroconvulsive Therapy in Schizophrenia

Wanhong Peng,Qingyu Tan,Minglan Yu,Ping Wang,Tingting Wang,Jixiang Yuan,Dongmei Liu,Dechao Chen,Chaohua Huang,Youguo Tan,Kezhi Liu,Bo Xiang,Xuemei Liang 대한신경정신의학회 2021 PSYCHIATRY INVESTIGATION Vol.18 No.5

Objective Schizophrenia (SCZ) is one of the most common and severe mental disorders. Modified electroconvulsive therapy (MECT) is the most effective therapy for all kinds of SCZ, and the underlying molecular mechanism remains unclear. This study is aim to detect the molecule mechanism by constructing the transcriptome dataset from SCZ patients treated with MECT and health controls (HCs).Methods Transcriptome sequencing was performed on blood samples of 8 SCZ (BECT: before MECT; AECT: after MECT) and 8 HCs, weighted gene co-expression network analysis (WGCNA) was used to cluster the different expression genes, enrichment and protein-protein interaction (PPI) enrichment analysis were used to detect the related pathways.Results Three gene modules (black, blue and turquoise) were significantly associated with MECT, enrichment analysis found that the long-term potentiation pathway was associated with MECT. PPI enrichment p-value of black, blue, turquoise module are 0.00127, <1×10^-16 and 1.09×10^-13, respectively. At the same time, EP300 is a key node in the PPI for genes in black module, which got from the transcriptome sequencing data.Conclusion It is suggested that the long-term potentiation pathways were associated with biological mechanism of MECT.

Reliable Localization for Wireless Sensor Networks in Complex Environments

Xiaolei Liu,Yongji Ren,Xuguang Xin,Liping Zhang,Jixiang Chen 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.10

Although localization has been widely studied for Wireless Sensor Networks (WSNs), the complex environments and the large network scale pose severe challenges and make it necessary to develop new reliable localization algorithms. In this paper, we propose a novel Multi-Hop Localization Algorithm for large-scale WSNs in complex environments. This work is based on the consideration that the localization process would encounter several kinds of adverse factors with different nature at the same time (e.g. anisotropic network characteristics, ranging uncertainty, link quality of multihop paths, etc.), which lead to obvious degradation of localization performance. Unlike most of the existing schemes, we transform the localization problem in complex environments into a hybrid constraint satisfaction problem (CSP) which is composed of three different kinds of constraints, i.e. spatial constraint, network situation constraint, and confidence constraint. Set-membership approach and interval analysis method have been utilized to deal with the CSP and determine the positions of sensor nodes. Simulation results show that our scheme is an effective and efficient approach to localization in large-scale WSNs.

Dynamic responses and robustness performance to moving boundary of double-stepped cable during deep-sea mining

Li Yilun,Guo Shuangxi,Guo Yucheng,Yu Xiaoqi,Chen Weimin,Song Jixiang 대한조선학회 2023 International Journal of Naval Architecture and Oc Vol.15 No.-

As the exploration and exploitation of deep-sea oil and gas, along with promising polymetallic nodule&sulfides mining, have been developing toward ultra-deep waters, some innovative concepts of marine cable configuration suitable for ultra-deepwater are proposed, such as stepped cable, hybrid cable and double-stepped cable. For deep-water cables with complex configurations, the structural responses become more complicated due to their non-uniform structural properties. Because the distributed buoyancy modules along cable length might introduce more significant local bending segments. Moreover, the impacts of moving boundary, caused by the motions of top vessel and bottom mining vehicle, should be considered. Through combing the finite element simulations with the hydrodynamic models, the dynamic response analysis approach of ultra-deepwater cables is established in this study. Then the double-stepped cable responses, including axial tension, displacement along with the change of overall configurations caused by moving top vessel and bottom mining vehicle, are calculated. Moreover, wave propagation behaviors during cable response are comprehensively examined, and the influences of non-uniform structural properties on cable response and wave propagation are analyzed using the wave propagation theory of structure with axially varying properties based on the Bessel function. The results show that the presented double-stepped cable can provide suitable configurations during the dynamic response, which has good compliance performance and can effectively buffer its response caused by moving boundary excitation. Finally, we found that the response spatial-temporal evolutions present some interesting phenomena, such as the axially non-uniform characteristics lead to non-monotonic changes in response amplitude and wavelength, with local peaks occurring in the low-tension region, owing to the distributed buoyancy modules, along with axiallyvarying and discontinuous structural properties. And, there exists significant mixed effect coming from both standing waves and traveling waves.