Anticancer effect of metformin on estrogen receptor-positive and tamoxifen-resistant breast cancer cell lines

KIM, JINKYOUNG,LEE, JIYUN,JANG, SOON YOUNG,KIM, CHUNGYEUL,CHOI, YOOJIN,KIM, AEREE NATIONAL HELLENIC RESEARCH FOUNDATION 2016 ONCOLOGY REPORTS Vol.35 No.5

<P>Acquisition of tamoxifen resistance (TR) during anti-estrogenic therapy using tamoxifen is a major obstacle in the treatment of estrogen receptor (ER)-positive breast cancer. As a biguanide derivative, metformin is commonly used to treat type II diabetes. It has recently emerged as a potential anticancer agent. The objective of the present study was to investigate the anticancer activity of metformin in relation to ERα expression and its signaling pathway in ERα-positive MCF-7 and MDA-MB-361 breast cancer cells as well as TR MCF-7 breast cancer cells. Metformin inhibited both protein and mRNA levels of ERα in the presence or absence of estrogen (E2) in the MCF-7, TR MCF-7 and MDA-MB-361 cells. Metformin repressed E2-inducible estrogen response element (ERE) luciferase activity, protein levels and mRNA levels of E2/ERα-regulated genes [including c-Myc, cyclin D1, progesterone receptor (PR) and pS2] to a greater degree than tamoxifen, resulting in inhibition of cell proliferation of MCF-7, TR MCF-7 and MDA-MB-361 cells. Collectively, our results suggest that one of the anticancer mechanisms of metformin could be attributable to the repression of expression and transcriptional activity of ERα. Metformin may be a good therapeutic agent for treating ERα-positive breast cancer by inhibiting the expression and function of ERα. In addition, metformin may be useful to treat tamoxifen-resistant breast cancer.</P>

GO-24 : Forkhead box P1 (Foxp1) in cervical cancer

( Hyeong Ju Kim ),( Ji Yun Lee ),( Jinkyoung Kong ),( Ji Hee Choi ),( Geum Seon Sohn ),( Eun Ji Nam ),( Sang Wun Kim ),( Doo Byung Chay ),( Sunghoon Kim ),( Jae Hoon Kim ),( Young Tae Kim ),( Han Byou 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: The forkhead box protein 1 (FOXP1) is considered as both a tumor suppressor candidate and a potential oncogene. Here, we investigated FOXP1 expression in cervical cancer, and the clinical significance of FOXP1 and it`s mechanism of action in cervical cancer. 방법: FOXP1`s functional role was investigated by employing lentiviral-mediated overexpression and knockdown in cervical cancer cell lines. Immunohistochemical staining for FOXP1 was performed on a cervical cancer tissue microarray consisting of 158 primary cervical cancers, 280 cervical intraepithelial neoplasias (CINs), and 378 matched normal tissues. 결과: FOXP1 overexpression promoted cell proliferation and tumorigenesis, whereas FOXP1 knockdown inhibited these properties in HeLa and CaSki cell lines. By immunohistochemical staining, FOXP1 expression increased during the normal to tumor transition of cervical carcinoma (p< 0.001), and this increased expression was significantly associated with tumor stage (p=0.009) and tumor grade (p< 0.001). In multivariate analysis, FOXP1+ (p=0.031) and tumor stage (p=0.032) were independent prognostic factors for overall survival. 결론: Taken together, our data indicate that FOXP1 has a crucial role in cervical cancer progression, and its overexpression is associated with poor prognosis, supporting that FOXP1 may be used as a promising novel target for therapeutic interventions.

GO-23 : Chemosensitivity testing based on gene expression profiling in patients with ovarian cancer

( Geum Seon Sohn ),( Hyeong Ju Kim ),( Ji Yun Lee ),( Jinkyoung Kong ),( Ji Hee Choi ),( Hanbyoul Cho ),( Eun Ji Nam ),( Sang Wun Kim ),( Sunghoon Kim ),( Jae Hoon Kim ),( Young Tae Kim ),( Doo Byung 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: To evaluate the association between clinical response of treatment agents and results of chemosensitivity testing in ovarian cancer. 방법: Tissue was obtained from 21 ovarian cancer patients and gene expression was evaluated by quantitative real-time polymerase chain reaction (PCR). Selected gene panel with expression of specific genes in the pathways that are related to drug responses in ovarian cancer were analyzed( AKT, Aurora A, BCRP, CD31, ERCC1, GSTpi, HER2, MDR1, Mitosin, PI3 Kinase, RRM1, Survivin, TOP1, TOP2A, TS, VEGF, VEGFR2, XIAP, P73). Gene expression were matched with therapeutic agent including Platinum, Taxanes, Bevacizumab, Gemcitabine, Topoisomerase I Inhibitors, Topoisomerase II Inhibitors, Cyclophosphamide, Herceptin, and 5-fluorouracil for chemosensitivity. 결과: Chemosensitivity testing revealed sensitivity rate of 66%, 81%, 96%, 56% and 61% for Platinum, Taxanes, Topoisomerase I Inhibitors, Topoisomerase II Inhibitors, and Bevacizumab, respectively. Treatment response rate was 70% (Complete Response: 40%, Partial Response: 30%). Treatment response was not significantly increased in the platinum sensitive patients (p=0.613), and overall response rate did not significantly differ according to the chemosensitivity test. 결론: This study may provide useful information in optimizing individual chemotherapy in the treatment of ovarian cancer.

Cloning of Dextranase Gene from Bacteroides xylanisolvens X3 isolated from Human Intestine and Characterization of its Enzyme

Jinkyoung Kim,Ling Li,Yu Mi Son,Nam Soo Han 한국당과학회 2014 한국당과학회 학술대회 Vol.2014 No.1

Analysis of Korean NEG-Morphemes in Terms of Transitivity

Kim, Jinkyoung 한국중앙영어영문학회 2009 영어영문학연구 Vol.51 No.1

This study analyzes the distribution and interpretations of negative forms in Korean from functionalists’ viewpoint of language, because they are heavily influenced by functions associated with constituents in a sentence/discourse. This study establishes a functional and discourse approach in the analysis of negation under the assumption that the interpretation of negation in Korean can be better explained by their functions in discourse. On the basis of this assumption, the distribution and interpretation of NEG-forms are examined in terms of transitivity. In addition, to examine why the two NEG-morphemes are so distributed, a transitivity theory is applied. In this theory, an can be regarded as an unmarked NEG-morpheme which negates predicates of either high or low transitivity, but mot must be regarded as a marked NEG-morpheme and is specialized to negate predicates of high transitivity only. The semantic interpretations of the NEG-morphemes an and mot then follow naturally as a consequence of derivation from the degree of transitivity each NEG-morpheme is associated with in the given predicate. In short, this study shows that the distribution and interpretation of NEG-forms cannot be explained in terms of purely syntactic form, but they can be explained better in terms of their functions in discourse.

Prerequisite Items of Spent Fuel Evaluation during Transportation and Interim Storage

Soyoung Kim,Yoonhee Lee,Sungki Lee,Yilsup Jung,Jinkyoung Kim,Sangjin Lee 한국방사성폐기물학회 2013 한국방사성폐기물학회 학술논문요약집 Vol.11 No.2

The utility of 2-antibody panel using MSH6 and PMS2 for screening of Lynch syndrome in endometrial cancer patients

( Jisup Kim ),( Jinkyoung Kong ),( Soohyn Kim ),( Min Ae Cho ),( Doo Byung Chay ),( Soon Won Hong ),( Jae-hoon Kim ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

목적: Lynch syndrome (LS) is an autosomal dominant genetic condition which increases the risk of developing colorectal, endometrial, and various other cancers. Inefficiency of selective screening method based on personal/family history has led to general use of universal screening method with 4-antibody panel (MLH1, MSH2, MSH6, PMS2) for detecting Mismatch Repair (MMR) gene deficiency. For cost-effectiveness of universal Lynch screening the use of 2-antibody panel composed of MSH6 and PMS2 have been suggested in patients with colorectal cancer. The purpose of this study is to evaluate the immunohistochemisrty results of missmatch repair gene expression in endometrial cancer. 방법: 120 endometrial carcinoma specimens derived from patients who performed hysterectomy from 2009 to 2016 were stained with 4-antibody panel (MLH1, MSH2, MSH6, PMS2) 결과: Overall, 49 out of 120 cases (40.8%) showed loss expression in at least one MMR genes. 16 case (21.3%) showed loss of MLH1 and PMS2. 11case (9.2%) showed loss of MSH2 and MSH6. 11 case (9.2%) showed loss of MSH6. 1 case showed loss of PMS2 expression. There was no case showing isolated loss of MLH1 or MSH2. 결론: Our finding proves the utility of 2-antibody panel (MSH6, PMS2) in screening of Lynch syndrome in endometrial cancer. Considering the cost-effectiveness, 2-antibody panel should be implemented for universal screening for Lynch syndrome in endometrial cancer patients.

서울지역 유통 다소비 식·약공용 농산물의 잔류농약 모니터링

김진경(Jinkyoung Kim),장미라(Mira Jang),윤용태(Yongtae Yoon),조성애(Sungae Jo),김은희(Eunhee Kim),신재민(Jaemin Shin),박영혜(Younghye Park),홍미선(Misun Hong),이성득(Sungdeuk Lee),신기영(Giyoung Shin),신용승(Yongseung Shin) 한국농약과학회 2021 농약과학회지 Vol.25 No.1

코로나19 시기 대학생의 학습경험 유형 및 특징 분석

김효원(Hyowon Kim),김누리(Nooree Kim),육진경(Jinkyoung Youk) 학습자중심교과교육학회 2023 학습자중심교과교육연구 Vol.23 No.4

목적 본 연구는 코로나19로 인해 비자발적 원격수업이 실시된 2020년과 2021년을 중심으로 대학생의 학습경험에 따라 유사한 특성을 갖는 잠재집단이 도출되는지와 변화양상을 탐색하는데 연구목적이 있다. 방법 이를 위하여 경기도와 전라남도 소재 2개 대학의 1~4학년을 대상으로 2020년과 2021년에 실시된 K-NSSE(학부교육실태)조사 결과를 활용하였다. 최종적으로 2020년에는 327명, 2021년에는 461명에 대한 자기주도학습전략, 유의미학습, 학업적 자기효능감, 학습동기 측정결과에 기초하여 잠재프로파일분석을 실시하여 유형화하였다. 그리고 도출된 집단 간 학습성과(핵심역량과 전공능력) 차이가 나타나는지 일원분산분석을 실시하여 확인하였다. 결과 그 결과는 다음과 같다. 첫째, 대학생의 학습상황에서 이루어지는 자기주도학습전략, 유의미학습, 학업적 자기효능감, 학습동기 그리고 학습성과인 핵심역량과 전공능력은 모두 유의한 정적 상관관계가 나타났다. 둘째, 대학생의 학습경험에 기초한 잠재집단을 탐색한 결과 2020년과 2021년 모두 ‘낮은 수준 학습경험형’, ‘중간 수준 학습경험형’, ‘높은 수준 학습경험형’의 3개 집단이 도출되었다. 셋째, 학습경험이 낮은 집단의 특징을 확인한 결과, 2020년은 새로운 원격교육 환경에서 인지적 어려움을 보인 반면, 2021년에는 원격교육 환경에 적응하면서 인지적 학습경험은 향상되었으나 학습동기와 학업적 자기효능감이 낮아진 경향성을 확인할 수 있었다. 마지막으로 2020년과 2021년 도출된 잠재집단에 따른 학습성과의 집단 간 차이를 살펴본 결과 핵심역량과 전공능력 모두 유의한 차이가 있는 것으로 확인되었다. 결론 연구결과를 토대로 본 연구에서는 코로나19로 지속된 원격수업 환경에서도 자기주도학습전략 및 학습기술, 학습동기가 학습성과를 높이는데 중요한 변인임을 확인할 수 있었다. 또한 이를 기반으로 대학생의 학습회복과 학습성과를 높여주기 위한 다양한 교육적 지원이 필요함을 확인하였다. Objectives This study explore whether latent classes with similar characteristics are derived according to the learning experiences of college students and their changes, focusing on 2020 and 2021, when involuntary distance classes were conducted due to COVID-19. Methods For this purpose, the results of the K-NSSE survey conducted in 2020 and 2021 targeting the first to fourth graders of two universities located in Gyeonggi-do and Jeolla-namdo were used. Finally, latent profile analysis was conducted based on the results of self-directed learning strategies, meaningful learning, academic self-efficacy, and learning motivation for 327 people in 2020 and 461 people in 2021. Also, one-way ANOVA analysis was performed to test the differences in educational outcomes between groups. Results The result are as follows. First, self-directed learning strategies, meaningful learning, academic self-efficacy, learning motivation, and core competencies and major skills, which are learning outcomes, all showed significant correlations in the learning situation of college students. Second, three latent groups were derived in 2020 and 2021 years : an ‘high-level learning experience’, a ‘medium-level learning experience’, and a ‘low-level learning experience’. Third, It was found that cognitive difficulties were shown in 2020 in the low-level learning experience type, while motivation and academic self-efficacy were lowered in 2021. Finally, as a result of examining the differences in learning outcomes between groups derived in 2020 and 2021, it was confirmed that there were significant differences in both core competencies and major skills. Conclusions Based on the results, it was confirmed that self-directed learning strategies, learning techniques, and learning motivation are important variables in improving students' learning outcomes even in the remote learning environment that has continued due to COVID-19. Also, it was suggested that various educational supports are needed to improve the learning recovery and learning outcomes of college students.

Optimization of RNA Extraction from Formalin-Fixed Paraffin-Embedded Blocks for Targeted Next-Generation Sequencing

Choi, Yoojin,Kim, Aeree,Kim, Jinkyoung,Lee, Jinhwan,Lee, Soo Yeon,Kim, Chungyeul 한국유방암학회 2017 Journal of breast cancer Vol.20 No.4

<P><B>Purpose</B></P><P>Breast cancer has a high prevalence in Korea. To achieve personalized therapy for breast cancer, long-term follow-up specimens are needed for next-generation sequencing (NGS) and multigene analysis. Formalin-fixed paraffin-embedded (FFPE) samples are easier to store than fresh frozen (FF) samples. The objective of this study was to optimize RNA extraction from FFPE blocks for NGS.</P><P><B>Methods</B></P><P>RNA quality from FF and FFPE tissues (n=5), expected RNA amount per unit area, the relationship between archiving time and quantity/quality of FFPE-extracted RNA (n=14), differences in quantitative real-time polymerase chain reaction (qRT-PCR) and NGS results, and comparisons of both techniques with tissue processing at different institutions (n=96) were determined in this study.</P><P><B>Results</B></P><P>The quality of RNA did not show any statistically significant difference between paired FF and FFPE specimens (<I>p</I>=0.49). Analysis of tumor cellularity gave an expected RNA amount of 33.25 ng/mm<SUP>2</SUP>. Archiving time affected RNA quality, showing a negative correlation with RNA integrity number and a positive correlation with threshold cycle. However, RNA from samples as old as 10 years showed a 100% success rate in qRT-PCR using short primers, showing that the effect of archiving time can be overcome by proper experiment design. NGS showed a higher success rate than qRT-PCR. Specimens from institution B (n=46), which were often stored in a refrigerator for more than 6 hours and fixed without slicing, showed lower success rates and worse results than specimens from the other institutes.</P><P><B>Conclusion</B></P><P>Archived FFPE tissues can be used to extract RNA for NGS if they are properly processed before fixation. The expected amount of RNA per unit size calculated in this study will be useful for other researchers.</P>